BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0435.0009.p (400 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value mll1470 [GEPR] COG0477 Permeases of the major facilitator superf... 196 5e-50 Cj0035c [GEPR] COG0477 Permeases of the major facilitator superf... 182 8e-46 RSc0216 [GEPR] COG0477 Permeases of the major facilitator superf... 159 7e-39 bcr [GEPR] COG0477 Permeases of the major facilitator superfamily 157 3e-38 PM1533 [GEPR] COG0477 Permeases of the major facilitator superfa... 143 6e-34 >mll1470 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 297 Score = 196 bits (498), Expect = 5e-50, Method: Compositional matrix adjust. Identities = 0/370 (0%), Positives = 0/370 (0%), Gaps = 10/370 (2%) Query: 23 GPFTVDMYLSSLPQLMSYFGTNTSMIXXXXXXXXXXXXXXXXXXXPISDVHGRRKPLLIS 82 + L ++ + S ++ + Sbjct: 19 IAPEKEQDLKTIVDDKKIIISVVSEPGFNIRVRKNESNNSHEIVLTVASLEYIWAFSNFF 78 Query: 83 MILYLLTSLACAFSPNIGLFIALRFIQGFVASAGLVISRAIVRDRYNGIEMTKFMSLLTM 142 + S + + ++ + + Sbjct: 79 WVFTQEYSKSQKNNDEHFDLTGKNRLKKSDELLKWARKNLQTTGCESWPKKCPKPEAYLQ 138 Query: 143 ISNIAPLISPTAGSTVTTFTSWXXXXXXXXXXXXXXXXXXXWGIKESLPAERRISSNFRE 202 S + + S + + A + I N E Sbjct: 139 GSEDSQVASEIFLCAIAWILHHEISHVVLQHPLVTTAFSTQEEREADSHATKWILGNLYE 198 Query: 203 VLRNYDSLLRDRSFMGYALVNGILFSGVFAYVAGTPFIYQNMYGISPQLFSILFALNGLG 262 + + + + + + IS + L Sbjct: 199 SAPELKKRALGIATAVLCIQSLEVENYFCLQNTHPAAYERIYSNISCYPVGNEELIEALC 258 Query: 263 IILGSQFVKRLAGRMDERRIFQIGLQLAFITTAAILMIVLAHGPFFALFISVFLFAAS-- 320 ++ ++ + Sbjct: 259 TVMLQYLFHGKNINVNLDGESFSSIL- 317 Query: 321 IGIIGPVSFTLAMESQGHIAGSAAAILGTLQFALGAVTSPLVGLGGDNSAIPF--GITIF 378 Sbjct: 318 -- 375 Query: 379 MTSILAVIAF 388 Sbjct: 376 --- 382 >Cj0035c [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 400 Score = 182 bits (462), Expect = 8e-46, Method: Compositional matrix adjust. Identities = 104/349 (29%), Positives = 174/349 (49%) Query: 23 GPFTVDMYLSSLPQLMSYFGTNTSMIXXXXXXXXXXXXXXXXXXXPISDVHGRRKPLLIS 82 P + DMYL +L + F TN+ + P+SD+ GR+ P L+ Sbjct: 26 APLSTDMYLPALSHVEQSFQTNSFLTQLSIASFFIAFALGQLIYGPLSDIFGRKIPALVG 85 Query: 83 MILYLLTSLACAFSPNIGLFIALRFIQGFVASAGLVISRAIVRDRYNGIEMTKFMSLLTM 142 + ++++SL C +I FIALRF + AG+VI+RAIV D + E +L+ + Sbjct: 86 IFFFIVSSLFCVIIDDIYAFIALRFFEALGGCAGVVIARAIVNDLFEIKEAAGIFALMMV 145 Query: 143 ISNIAPLISPTAGSTVTTFTSWXXXXXXXXXXXXXXXXXXXWGIKESLPAERRISSNFRE 202 S++AP++SPT G + + SW +G+KES P + + E Sbjct: 146 FSSLAPMLSPTFGGILLEYFSWHSIFATLFALGILLFLMILFGLKESAPHLKNKKFSHHE 205 Query: 203 VLRNYDSLLRDRSFMGYALVNGILFSGVFAYVAGTPFIYQNMYGISPQLFSILFALNGLG 262 +++Y +L D+ F+ Y L + +FAY+ G+ F++ + +S Q F++LF N LG Sbjct: 206 AMKSYKFVLSDKRFLVYILCASFALAAMFAYITGSSFVFTQFFSLSEQKFALLFGANALG 265 Query: 263 IILGSQFVKRLAGRMDERRIFQIGLQLAFITTAAILMIVLAHGPFFALFISVFLFAASIG 322 ++ + RL + + +I L + FI+T +L+ H F +S+F A +G Sbjct: 266 FVICANINARLVLKYESEKILAKALMIMFISTVILLVNAFFHPNFLLFELSIFTSIAMLG 325 Query: 323 IIGPVSFTLAMESQGHIAGSAAAILGTLQFALGAVTSPLVGLGGDNSAI 371 I P + TLAM +G+A+A+LGT+QF + S +VG N+ I Sbjct: 326 FIAPNTTTLAMARFKEHSGTASAVLGTVQFGFAGLISFVVGAINANTPI 374 >RSc0216 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 409 Score = 159 bits (402), Expect = 7e-39, Method: Compositional matrix adjust. Identities = 110/373 (29%), Positives = 175/373 (46%), Gaps = 4/373 (1%) Query: 26 TVDMYLSSLPQLMSYFGTNTSMIXXXXXXXXXXXXXXXXXXXPISDVHGRRKPLLISMIL 85 + D+YL ++P + + G + + PI D GRR P+ + ++L Sbjct: 29 STDLYLPAMPAMQATLGASAGALEWTISGYLVGFSLGQLLWGPIGDRTGRRLPIALGLLL 88 Query: 86 YLLTSLACAFSPNIGLFIALRFIQGFVASAGLVISRAIVRDRYNGIEMTKFMSLLTMISN 145 ++ S CA + + IA R +Q A A +V++RA+VRD Y G + MS L + Sbjct: 89 FIGGSAGCALAASAPALIAWRAVQAAGACASVVLARAMVRDLYEGERAARMMSTLMTVMA 148 Query: 146 IAPLISPTAGSTVTTFTSWXXXXXXXXXXXXXXXXXXXWGIKESLPAERRISSNFREVLR 205 IAPL+ P+AG + SW + E+LPA RR + L Sbjct: 149 IAPLVGPSAGGLILKLWSWRAIFWTLVIVGLATLAAVG-TLPETLPAHRRNDAPLHRTLA 207 Query: 206 NYDSLLRDRSFMGYALVNGILFSGVFAYVAGTPFIYQNMYGISPQLFSILFALNGLGIIL 265 Y LLR +GY V G + G++AYVAGTPF Y + Y + Q + +LF L +GI+ Sbjct: 208 RYGVLLRHPRLLGYLGVGGFFYGGMYAYVAGTPFAYIDYYHVPAQHYGLLFGLGIVGIMA 267 Query: 266 GSQFVKRLAGRMDERRIFQIGLQLAFITTAAILMIV-LAHGPFFALFISVFLFAASIGII 324 + L GR R+ + G A + AA+ + + G L I +F+F ++ G I Sbjct: 268 TNLLNANLVGRFGGDRLMRAGAVAAALAGAAVALAARMGWGGLMGLVIPLFVFVSTTGFI 327 Query: 325 GPVSFTLAMESQGHIAGSAAAILGTLQFALGAVTSPLVGLGGDNSAIPFGITIFMTSILA 384 + T A+ + AGS +A++G Q+ G + S LVG + + +P G+ I Sbjct: 328 VANAMTGALGTFPQFAGSVSALVGATQYGTGILGSALVGALANGTPVPMGLVIAACGSGG 387 Query: 385 VIAFVFLV--KKP 395 +A V LV ++P Sbjct: 388 ALAAVLLVPARRP 400 >bcr [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 396 Score = 157 bits (397), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 97/349 (27%), Positives = 176/349 (50%) Query: 24 PFTVDMYLSSLPQLMSYFGTNTSMIXXXXXXXXXXXXXXXXXXXPISDVHGRRKPLLISM 83 P ++DMYL +LP + + FG P++D GR+ +L Sbjct: 23 PLSIDMYLPALPVISAQFGVPAGSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGT 82 Query: 84 ILYLLTSLACAFSPNIGLFIALRFIQGFVASAGLVISRAIVRDRYNGIEMTKFMSLLTMI 143 +++ ++ACA + I I +RF G A+A V+ A++RD Y E ++ MS + ++ Sbjct: 83 LVFAAAAVACALANTIDQLIVMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLV 142 Query: 144 SNIAPLISPTAGSTVTTFTSWXXXXXXXXXXXXXXXXXXXWGIKESLPAERRISSNFREV 203 + IAPL++P G V + SW + IKE+LP ERR + R Sbjct: 143 TTIAPLMAPIVGGWVLVWLSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTT 202 Query: 204 LRNYDSLLRDRSFMGYALVNGILFSGVFAYVAGTPFIYQNMYGISPQLFSILFALNGLGI 263 + N+ +L R + + Y L +G F+G+F++++ PF+Y + ++P+ F FALN + + Sbjct: 203 IGNFAALFRHKRVLSYMLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFL 262 Query: 264 ILGSQFVKRLAGRMDERRIFQIGLQLAFITTAAILMIVLAHGPFFALFISVFLFAASIGI 323 + + F R R+ +F+ GL + FI A +++ L F++L + V F + + Sbjct: 263 FVMTIFNSRFVRRIGALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSM 322 Query: 324 IGPVSFTLAMESQGHIAGSAAAILGTLQFALGAVTSPLVGLGGDNSAIP 372 + + + ++ H+AG+A+++ GT +F +GA+ L+ L NSA P Sbjct: 323 VSSNAMAVILDEFPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWP 371 >PM1533 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 399 Score = 143 bits (360), Expect = 6e-34, Method: Compositional matrix adjust. Identities = 99/377 (26%), Positives = 176/377 (46%), Gaps = 2/377 (0%) Query: 23 GPFTVDMYLSSLPQLMSYFGTNTSMIXXXXXXXXXXXXXXXXXXXPISDVHGRRKPLLIS 82 GP ++DMYL SLP + T+ + P+SD +GRR LLI Sbjct: 25 GPLSIDMYLPSLPTIAKALHTSIEWVQLSVSTFLTGFCVGMLFYGPLSDKYGRRIILLIG 84 Query: 83 MILYLLTSLACAFSPNIGLFIALRFIQGFVASAGLVISRAIVRDRYNGIEMTKFMSLLTM 142 M +Y++ S+AC+ + +I + RF+Q F G V+ RAIVRD + +T +S++ + Sbjct: 85 MTIYIVASIACSIATSIEQLLVARFLQAFGGGVGPVLGRAIVRDSFPPQRITHVLSMMQL 144 Query: 143 ISNIAPLISPTAGSTVTTFTSWXXXXXXXXXXXXXXXXXXXWGIKESLPAERRISSNFRE 202 ++ +APL++P G V + W + + E+ + N Sbjct: 145 VTMLAPLLAPFIGGIVLLWFGWETQFLLLALIGVVCWLIAYFFLAETNQDRHQRPLNLGT 204 Query: 203 VLRNYDSLLRDRSFMGYALVNGILFSGVFAYVAGTPFIYQNMYGISPQLFSILFALNGLG 262 + Y +L+ G L +F G+FAYVAGTPF+Y + + I PQ + F +N +G Sbjct: 205 FFQAYCGILKQPQSFGNILCLSAMFGGMFAYVAGTPFVYIDYFHIPPQDYGFYFGINVVG 264 Query: 263 IILGSQFVKRLAGRMDERRIFQIGLQLAFITTAAILMIVLAHGPFFALFISVFLFAASIG 322 I+L + R +R+ ++ + A + + FA+ + +F+F G Sbjct: 265 IVLATLINNYCVKRYAVQRVLMA--EMGLVALAGMAFFLADPDSLFAIMLPLFIFVGLTG 322 Query: 323 IIGPVSFTLAMESQGHIAGSAAAILGTLQFALGAVTSPLVGLGGDNSAIPFGITIFMTSI 382 + P T ++ H AG+A A++ ++QFA G V S + +NS + + Sbjct: 323 ALTPNVMTSLLKQHSHAAGAAMALVASMQFAGGFVASGALSYFFNNSPQTMLWVMSTCAG 382 Query: 383 LAVIAFVFLVKKPTLAS 399 +A++ F+ +K +++S Sbjct: 383 VAIVGFMITLKPNSISS 399 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.328 0.142 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,528,661 Number of extensions: 833863 Number of successful extensions: 3122 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 3108 Number of HSP's successfully gapped: 26 Length of query: 400 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 291 Effective length of database: 25,807,025 Effective search space: 7509844275 Effective search space used: 7509844275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 117 (49.7 bits)