BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0435.0008.p (161 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SMc00680 [R] COG1064 Zn-dependent alcohol dehydrogenases 222 2e-58 AGc3663A [R] COG1064 Zn-dependent alcohol dehydrogenases 214 6e-56 Rv3045 [R] COG1064 Zn-dependent alcohol dehydrogenases 213 8e-56 MT3130 [R] COG1064 Zn-dependent alcohol dehydrogenases 213 8e-56 ML1730 [R] COG1064 Zn-dependent alcohol dehydrogenases 210 9e-55 >SMc00680 [R] COG1064 Zn-dependent alcohol dehydrogenases Length = 347 Score = 222 bits (565), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 107/159 (67%), Positives = 127/159 (79%) Query: 1 MAVKLAHAMGAEVTVLSQTLKKKEDGLKLGADHYYATSDPETFKKLTGKFDLIVNTVSAK 60 M VKLAHAMGAEVTVLSQTL KKEDGLKLGADHYYAT DPETF KL G FDLI+ TV+A+ Sbjct: 188 MGVKLAHAMGAEVTVLSQTLSKKEDGLKLGADHYYATKDPETFTKLAGSFDLIICTVAAE 247 Query: 61 IDLDAYLSLLAVNGTLVNVGAPPEALPIQVFSLIGHRRSFAGSAIGGIRETQEMLDFCAK 120 ID +AYL+LL V+G LV VG P +P+ FSL+ RRS +GS IG I+ETQEMLDFC + Sbjct: 248 IDWNAYLNLLKVDGELVLVGIPENPVPVHAFSLVPARRSISGSMIGSIKETQEMLDFCGE 307 Query: 121 HHITPEIEVISANQIDQAWERVLASDVRYRFVIDISTME 159 H I EIE I QI++A+ERV+ SDVRYRFVID+ +++ Sbjct: 308 HGIVSEIETIRMEQINEAYERVVRSDVRYRFVIDMESIK 346 >AGc3663A [R] COG1064 Zn-dependent alcohol dehydrogenases Length = 289 Score = 214 bits (544), Expect = 6e-56, Method: Compositional matrix adjust. Identities = 6/159 (3%), Positives = 23/159 (14%) Query: 1 MAVKLAHAMGAEVTVLSQTLKKKEDGLKLGADHYYATSDPETFKKLTGKFDLIVNTVSAK 60 H + + E+G + ++ G V Sbjct: 82 AYRLADHPDALAEELGAANTIWMEEGKLHATNTDGYGFVSNLDERHPGWDKTQRAVVFGA 141 Query: 61 IDLDAYLSLLAVNGTLVNVGAPPEALPIQVFSLIGHRRSFAGSAIGGIRETQEMLDFCAK 120 + + + + + ++E Sbjct: 142 GGASRAVIQSLRDRDVAEIHVVNRTVERARELADRFGPRVFSHPQAALQEVMHGAGLFVN 201 Query: 121 HHITPEIEVISANQIDQAWERVLASDVRYRFVIDISTME 159 + + + + Sbjct: 202 TTSLGMNGTEAPHLDFSHLAANAVVTDIVYVPLKTPILN 240 >Rv3045 [R] COG1064 Zn-dependent alcohol dehydrogenases Length = 346 Score = 213 bits (543), Expect = 8e-56, Method: Compositional matrix adjust. Identities = 104/158 (65%), Positives = 126/158 (79%) Query: 1 MAVKLAHAMGAEVTVLSQTLKKKEDGLKLGADHYYATSDPETFKKLTGKFDLIVNTVSAK 60 M VKL AMGA+VTVLSQ+LKK EDGL+LGA YYAT+DP+TF+KL G FDLI+NTVSA Sbjct: 189 MGVKLGAAMGADVTVLSQSLKKMEDGLRLGAKSYYATADPDTFRKLRGGFDLILNTVSAN 248 Query: 61 IDLDAYLSLLAVNGTLVNVGAPPEALPIQVFSLIGHRRSFAGSAIGGIRETQEMLDFCAK 120 +DL YL+LL V+GTLV +G P + + F+L RRS AGS IGGI ETQEML+FCA+ Sbjct: 249 LDLGQYLNLLDVDGTLVELGIPEHPMAVPAFALALMRRSLAGSNIGGIAETQEMLNFCAE 308 Query: 121 HHITPEIEVISANQIDQAWERVLASDVRYRFVIDISTM 158 H +TPEIE+I + I+ A+ERVLASDVRYRFVIDIS + Sbjct: 309 HGVTPEIELIEPDYINDAYERVLASDVRYRFVIDISAL 346 >MT3130 [R] COG1064 Zn-dependent alcohol dehydrogenases Length = 346 Score = 213 bits (543), Expect = 8e-56, Method: Compositional matrix adjust. Identities = 104/158 (65%), Positives = 126/158 (79%) Query: 1 MAVKLAHAMGAEVTVLSQTLKKKEDGLKLGADHYYATSDPETFKKLTGKFDLIVNTVSAK 60 M VKL AMGA+VTVLSQ+LKK EDGL+LGA YYAT+DP+TF+KL G FDLI+NTVSA Sbjct: 189 MGVKLGAAMGADVTVLSQSLKKMEDGLRLGAKSYYATADPDTFRKLRGGFDLILNTVSAN 248 Query: 61 IDLDAYLSLLAVNGTLVNVGAPPEALPIQVFSLIGHRRSFAGSAIGGIRETQEMLDFCAK 120 +DL YL+LL V+GTLV +G P + + F+L RRS AGS IGGI ETQEML+FCA+ Sbjct: 249 LDLGQYLNLLDVDGTLVELGIPEHPMAVPAFALALMRRSLAGSNIGGIAETQEMLNFCAE 308 Query: 121 HHITPEIEVISANQIDQAWERVLASDVRYRFVIDISTM 158 H +TPEIE+I + I+ A+ERVLASDVRYRFVIDIS + Sbjct: 309 HGVTPEIELIEPDYINDAYERVLASDVRYRFVIDISAL 346 >ML1730 [R] COG1064 Zn-dependent alcohol dehydrogenases Length = 362 Score = 210 bits (534), Expect = 9e-55, Method: Compositional matrix adjust. Identities = 101/158 (63%), Positives = 126/158 (79%) Query: 1 MAVKLAHAMGAEVTVLSQTLKKKEDGLKLGADHYYATSDPETFKKLTGKFDLIVNTVSAK 60 M VKL MG EVTVLSQ+LKK EDGL+LGA +YYAT+DP+TF+KL G FDLI+NTVSA Sbjct: 205 MGVKLGRKMGCEVTVLSQSLKKMEDGLQLGASNYYATADPDTFRKLRGSFDLILNTVSAN 264 Query: 61 IDLDAYLSLLAVNGTLVNVGAPPEALPIQVFSLIGHRRSFAGSAIGGIRETQEMLDFCAK 120 +DL YL+LL VNGTLV +G P + + F+L RRS AGS IGGI ETQEM++F A+ Sbjct: 265 LDLGKYLNLLDVNGTLVELGIPEHPMLVPAFALALMRRSLAGSNIGGITETQEMMNFSAE 324 Query: 121 HHITPEIEVISANQIDQAWERVLASDVRYRFVIDISTM 158 H +TPEIE+I + I++A+ERVLASDVRYRFVI+IS++ Sbjct: 325 HSVTPEIELIEPDYINEAYERVLASDVRYRFVINISSL 362 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.134 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 11,220,888 Number of extensions: 416184 Number of successful extensions: 2654 Number of sequences better than 1.0e-05: 44 Number of HSP's gapped: 2629 Number of HSP's successfully gapped: 44 Length of query: 161 Length of database: 38,378,431 Length adjustment: 98 Effective length of query: 63 Effective length of database: 27,075,699 Effective search space: 1705769037 Effective search space used: 1705769037 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 111 (47.4 bits)