BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0435.0006.p (274 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value MA0409 [S] COG0599 Uncharacterized homolog of gamma-carboxymucon... 281 7e-76 SMa0316 [S] COG0599 Uncharacterized homolog of gamma-carboxymuco... 87 4e-17 SMa0559 [S] COG0599 Uncharacterized homolog of gamma-carboxymuco... 85 1e-16 L35675 [S] COG0599 Uncharacterized homolog of gamma-carboxymucon... 78 2e-14 MTH234 [S] COG0599 Uncharacterized homolog of gamma-carboxymucon... 67 4e-11 >MA0409 [S] COG0599 Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit Length = 250 Score = 281 bits (720), Expect = 7e-76, Method: Compositional matrix adjust. Identities = 135/250 (54%), Positives = 173/250 (69%) Query: 24 MNRIEKSKETYKRLFGDGVPTAYATDPDLQEILSRFIFGEVFDQGKLDDKQRELITLVVL 83 M E + +K LF D T TDP+L E+ F F EV G LD K R ++ L L Sbjct: 1 MRISETANRNHKELFPDHKSTLKVTDPELIEVFDNFAFDEVISYGNLDTKTRMMVILGSL 60 Query: 84 AANQTLPQLKAHANAALNVGLTPVEIKEAIYQCAPYLGFPKTLNAANKVNEVFKARNIEL 143 A+QTL + K ALNVG+TP+E+KE +YQ PY+G K + + NE+ ++R IEL Sbjct: 61 IASQTLSEYKVMLGGALNVGVTPIEVKEIVYQSVPYVGIAKIFDFIHATNEILESRGIEL 120 Query: 144 PLESQTQVTDDTRLEKGLAVQTEIFGDNVAKMHGSAPDNQKHIQDYLSAFCFGDFYTRNG 203 PLE Q+ + +TR EKGL VQ EIFGD + KM+ +AP+NQ HIQ YLSA CFGD+YTR G Sbjct: 121 PLEGQSTTSPETRFEKGLEVQKEIFGDIIDKMYEAAPENQIHIQKYLSANCFGDYYTRKG 180 Query: 204 LDLQTRELLTLCIISALGGAEGQVKAHVQGNKNVGNDKETLIAAITHCLPYMGFPRTLNA 263 LD++TRELLT +I ALGG E Q+K H+QGN NVGNDK+TL++ +T LPY+G+PRTLNA Sbjct: 181 LDVKTRELLTFSMILALGGCEPQLKGHIQGNLNVGNDKKTLLSTVTQLLPYIGYPRTLNA 240 Query: 264 IANINEVIPE 273 IA +NEVIPE Sbjct: 241 IACLNEVIPE 250 >SMa0316 [S] COG0599 Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit Length = 420 Score = 86.7 bits (213), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 63/217 (29%), Positives = 97/217 (44%), Gaps = 10/217 (4%) Query: 50 PDLQEILSRFIFGEVFDQGKLDDKQRELITLVVLAANQTL-PQLKAHANAALNVGLTPVE 108 PD + E+F L+ + L + +LAA + AH +AL G T + Sbjct: 170 PDFVNWREHTLGEEIFGAVHLEPRLAHLASAAMLAARGKVGASFDAHIVSALAAGATDSD 229 Query: 109 IKEAIYQCAPYLGFPKTLNAANKVNEVFKARNIELPLES-----QTQVTDDTRLEKGLAV 163 I E + Q + Y GFP LNAA + V A E P S T D+ R +G+A Sbjct: 230 IVEMVIQLSVYSGFPAALNAAGRARNVLAAP--ERPQASTQKGVDTTKDDEKRFMRGVAT 287 Query: 164 QTEIFGDNVAKMHGSAPDNQKHIQDYLSAFCFGDFYTRNGLDLQTRELLTLCIISALG-- 221 E G + A + S D + + A C+GD + R LD + REL + ++A G Sbjct: 288 LAETSGGSGADVVDSFKDVAPGLGRLIVAHCYGDIFCRPALDPKVRELGAISALAAQGTV 347 Query: 222 GAEGQVKAHVQGNKNVGNDKETLIAAITHCLPYMGFP 258 AE + H+ N+G E ++ + + +PY G+P Sbjct: 348 AAEKPLGVHIDAALNLGATPEEIVETLFNIIPYAGYP 384 Score = 63.9 bits (154), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 36/134 (26%), Positives = 68/134 (50%) Query: 140 NIELPLESQTQVTDDTRLEKGLAVQTEIFGDNVAKMHGSAPDNQKHIQDYLSAFCFGDFY 199 +IE LE+ D + +GLAV +I G+N + + + + +GD Sbjct: 5 HIEQQLENLASPGDRQKHLRGLAVLKQIGGENFGGPVSQLASFSEDLARFTIQYPYGDVL 64 Query: 200 TRNGLDLQTRELLTLCIISALGGAEGQVKAHVQGNKNVGNDKETLIAAITHCLPYMGFPR 259 +R+GLDL+TR++LT + A G A+ Q+ H+ G N G + ++ + +GFP Sbjct: 65 SRDGLDLRTRQILTAATLLAHGSAQSQLSFHLYGLLNAGGTRADVVDLLFISAGLLGFPT 124 Query: 260 TLNAIANINEVIPE 273 +NA+ + +++ + Sbjct: 125 AINAVPIVRDILAD 138 Score = 58.5 bits (140), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 55/238 (23%), Positives = 93/238 (39%), Gaps = 21/238 (8%) Query: 38 FGDGVPTAYATDPDLQEILSRFIFGEVFDQGKLDDKQRELITLVVLAANQTL-PQLKAHA 96 FG V + DL ++ +G+V + LD + R+++T L A+ + QL H Sbjct: 37 FGGPVSQLASFSEDLARFTIQYPYGDVLSRDGLDLRTRQILTAATLLAHGSAQSQLSFHL 96 Query: 97 NAALNVGLTPVEIKEAIYQCAPYLGFPKTLNAANKVNEVFKARNIELPLESQTQVTDDTR 156 LN G T ++ + ++ A LGFP +NA V ++ R D+T+ Sbjct: 97 YGLLNAGGTRADVVDLLFISAGLLGFPTAINAVPIVRDILADR-------------DETK 143 Query: 157 LEKGLAVQ---TEIFGDNVAKMHGSAPDNQKHIQDYLSAFCFGDFYTRNGLDLQTRELLT 213 G ++ D A + APD + L FG + L+ + L + Sbjct: 144 NVAGAQTSPAVPDMPSDRFAVLDRIAPDFVNWREHTLGEEIFGAVH----LEPRLAHLAS 199 Query: 214 LCIISALGGAEGQVKAHVQGNKNVGNDKETLIAAITHCLPYMGFPRTLNAIANINEVI 271 +++A G AH+ G ++ + Y GFP LNA V+ Sbjct: 200 AAMLAARGKVGASFDAHIVSALAAGATDSDIVEMVIQLSVYSGFPAALNAAGRARNVL 257 Score = 56.2 bits (134), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 34/99 (34%), Positives = 52/99 (52%), Gaps = 6/99 (6%) Query: 50 PDLQEILSRFIFGEVFDQGKLDDKQRELITLVVLAANQTLPQ---LKAHANAALNVGLTP 106 P L ++ +G++F + LD K REL + LAA T+ L H +AALN+G TP Sbjct: 308 PGLGRLIVAHCYGDIFCRPALDPKVRELGAISALAAQGTVAAEKPLGVHIDAALNLGATP 367 Query: 107 VEIKEAIYQCAPYLGFP---KTLNAANKVNEVFKARNIE 142 EI E ++ PY G+P K L A+ +F + ++ Sbjct: 368 EEIVETLFNIIPYAGYPLIEKALLIAHDRISLFDEKQVD 406 >SMa0559 [S] COG0599 Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit Length = 259 Score = 84.7 bits (208), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 61/218 (27%), Positives = 102/218 (46%), Gaps = 14/218 (6%) Query: 50 PDLQEILSRFIFGEVFDQGKLDDKQRELITLVVLAANQTLPQLKAHANAALNVGLTPVEI 109 P L++ + ++G+V+ + L+ + R L+T+ L A P L +A+ AL G+ P EI Sbjct: 41 PALEQYTQQRLYGDVWQRPGLNRRDRSLVTIAALIARGEAPALTYYADQALENGVKPSEI 100 Query: 110 KEAIYQCAPYLGFPKTLNAANKVNEVFKARNIELPLESQTQVTDDTRL---EKGLAVQTE 166 E I A Y G+ K + V+E F R I + Q + T L E+ A + Sbjct: 101 SETITHLAYYSGWGKAMATVGPVSEAFAKRGIG---QDQLAAVESTPLPLDEEAEAQRAT 157 Query: 167 IFGDNVAKMHGSAPDNQKHIQDYLSAFCFGDFYTRNGLDLQTRELLTLCIISALGGAEGQ 226 G+ + AP ++ DYL F D + R L + R L+T+ + ++G E Q Sbjct: 158 TVGNQFGSV---APGLVQYTTDYL----FRDLWLRPDLAPRDRSLVTIAALISVGQVE-Q 209 Query: 227 VKAHVQGNKNVGNDKETLIAAITHCLPYMGFPRTLNAI 264 + H+ + G +E ITH Y G+P ++A+ Sbjct: 210 ITFHLNKALDNGLSEEQAAEVITHLAFYAGWPNAMSAL 247 >L35675 [S] COG0599 Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit Length = 122 Score = 77.8 bits (190), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 40/91 (43%), Positives = 55/91 (60%), Gaps = 1/91 (1%) Query: 50 PDLQEILSRFIFGEVFDQGKLDDKQRELITLV-VLAANQTLPQLKAHANAALNVGLTPVE 108 PDL ++ +F FG ++++G L+ K RELIT+ +L QL+ H A+LNVGLT E Sbjct: 31 PDLNNLMLKFAFGTIYERGVLNFKDRELITITSLLTQGGCENQLRVHIQASLNVGLTQEE 90 Query: 109 IKEAIYQCAPYLGFPKTLNAANKVNEVFKAR 139 I E APY+GFP+ LNA E+F R Sbjct: 91 IIETFIHYAPYVGFPRVLNAIFVAKEIFTDR 121 Score = 75.9 bits (185), Expect = 6e-14, Method: Compositional matrix adjust. Identities = 41/118 (34%), Positives = 62/118 (52%), Gaps = 8/118 (6%) Query: 164 QTEIFGDNVAKMHGSAP--------DNQKHIQDYLSAFCFGDFYTRNGLDLQTRELLTLC 215 + EI N+AK+ G+A D + + + F FG Y R L+ + REL+T+ Sbjct: 3 KKEIGLKNLAKIDGAAAEVVFDSLKDLAPDLNNLMLKFAFGTIYERGVLNFKDRELITIT 62 Query: 216 IISALGGAEGQVKAHVQGNKNVGNDKETLIAAITHCLPYMGFPRTLNAIANINEVIPE 273 + GG E Q++ H+Q + NVG +E +I H PY+GFPR LNAI E+ + Sbjct: 63 SLLTQGGCENQLRVHIQASLNVGLTQEEIIETFIHYAPYVGFPRVLNAIFVAKEIFTD 120 >MTH234 [S] COG0599 Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit Length = 337 Score = 66.6 bits (161), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 7/87 (8%), Positives = 18/87 (20%) Query: 50 PDLQEILSRFIFGEVFDQGKLDDKQRELITLVVLAANQTLPQLKAHANAALNVGLTPVEI 109 P + E + G + + + T + + T + Sbjct: 31 PQVDEPGLLALAGYKAGMTHVMMVDNQKNSPTEGMEVSTPVTILEVPPLTVMAVRTYEKT 90 Query: 110 KEAIYQCAPYLGFPKTLNAANKVNEVF 136 + L + K+ Sbjct: 91 SRGLKTLGEVLATETKDDLRRKLTPPA 117 Score = 60.5 bits (145), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 4/119 (3%), Positives = 19/119 (15%), Gaps = 1/119 (0%) Query: 156 RLEKGLAVQTEIFGDNVAKMHGSAPDNQKHIQDYLSAFCFGDFYTRNGLDLQTRELLTLC 215 R + A + + + G + + + + Sbjct: 3 RHHQPRKGSVAFSPRKRAARETPRVKSWPQVDEPGLLALAGYKAGMTHVMMVDNQKNSPT 62 Query: 216 IISALGGAEGQVKAHVQGNKNVGNDKETLIAAITHCLPYMGFPRTLNAIANINEVIPES 274 + + E + + + + + Sbjct: 63 EGMEVS-TPVTILEVPPLTVMAVRTYEKTSRGLKTLGEVLATETKDDLRRKLTPPADDY 120 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,999,099 Number of extensions: 763537 Number of successful extensions: 1462 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 1447 Number of HSP's successfully gapped: 28 Length of query: 274 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 169 Effective length of database: 26,268,361 Effective search space: 4439353009 Effective search space used: 4439353009 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)