BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0435.0005.p (321 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1225 [C] COG0667 Predicted oxidoreductases (related to aryl-a... 154 2e-37 DR2261 [C] COG0667 Predicted oxidoreductases (related to aryl-al... 132 6e-31 AGc4474 [F] COG0788 Formyltetrahydrofolate hydrolase 132 1e-30 AGc447 [C] COG0667 Predicted oxidoreductases (related to aryl-al... 132 1e-30 PA2535 [C] COG0667 Predicted oxidoreductases (related to aryl-al... 132 1e-30 >all1225 [C] COG0667 Predicted oxidoreductases (related to aryl-alcohol dehydrogenases) Length = 346 Score = 154 bits (388), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 18/286 (6%), Positives = 48/286 (16%), Gaps = 16/286 (5%) Query: 33 FGNYLTASDLKPVFDKAMEAGFNLWDTAAVYGIGASETILGNFTK-GRKDVLISTKFTPQ 91 + + + A + K G ++ LI Sbjct: 31 DAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSG 90 Query: 92 IAGDSENAVEELLDGSLKRLGVDHADIYWIHNPADVQRWTPKLIPLM----KSGKVKYVG 147 + +++ + K + D + + + Sbjct: 91 PSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTD 150 Query: 148 VSNHNLEEIKWAASIFAEEGLKISAVQNHYSLLYRSSEEAGILDYCKENGMIFFSYMVLE 207 + A + KI + + + D ++ Sbjct: 151 SAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSL 210 Query: 208 QGALTDKYNAQHPFPSDTRRGEAFNIDVFVKLEGL------IQVMRDIGTKYNATTAQVA 261 + A+ G + L + + + Sbjct: 211 LNRSFEVGLAE----VSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYS 266 Query: 262 IAWSIAKGTV-PIIGVTKTTHIEDAVKASKLSLTAQEISDLEAVAK 306 + I A + L A Sbjct: 267 GEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTM 312 >DR2261 [C] COG0667 Predicted oxidoreductases (related to aryl-alcohol dehydrogenases) Length = 346 Score = 132 bits (333), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 13/292 (4%), Positives = 32/292 (10%), Gaps = 29/292 (9%) Query: 33 FGNYLTASDLKPVFDKAMEAGFNLWDTAAVYGIGASETILGNFTKGRKD-VLISTKF--- 88 A + + Sbjct: 28 SEADAHAQLDY-AVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIAS 86 Query: 89 ----TPQIAGDSENA--VEELLDGSLKRLGVDHADIYWIHNPADVQRWTP------KLIP 136 + + + + + + Sbjct: 87 KVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLD---LYQVHWPQRPTNCFGK 143 Query: 137 LMKSGKVKYVGVSNHNLEEIKWAASIFAEEGLKISAVQNHYSLLYRSSEEAGILDYCKEN 196 L S VS + + + L + Sbjct: 144 LGYSWTDSAPAVSLLDTLDALAEYQRAG-----KIRYIGVSNETAFGVMRYLHLADKHDL 198 Query: 197 GMIFFSYMVLEQGALTDKYNAQHPF-PSDTRR-GEAFNIDVFVKLEGLIQVMRDIGTKYN 254 I + + + + + L Sbjct: 199 PRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTL 258 Query: 255 ATTAQVAIAWSIAKGT--VPIIGVTKTTHIEDAVKASKLSLTAQEISDLEAV 304 + K I A + L A Sbjct: 259 FSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGAT 310 >AGc4474 [F] COG0788 Formyltetrahydrofolate hydrolase Length = 86 Score = 132 bits (331), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 0/278 (0%), Positives = 0/278 (0%), Gaps = 27/278 (9%) Query: 48 KAMEAGFNLWDTAAVYGIGASETILGNFTKGRKD-VLISTKFTPQI-----AG------D 95 Sbjct: 77 136 Query: 96 S--ENAVEELLDGSLKRLGVDHADIYWIH--NP-ADVQRWTPKLIPLMKSGKVKYVGVSN 150 Sbjct: 137 - 195 Query: 151 HNLEEIKWAASIFAEEGLKISAVQNHYSLLYRSSEEAGILDYCKENGMIFFSYMVLEQGA 210 Sbjct: 196 ----- 250 Query: 211 LTDKYNAQHPFPSDT-RRG-EAFNIDVFVKLEGLIQVMRDIGTKYNATTAQVAIAWSIAK 268 Sbjct: 251 310 Query: 269 G--TVPIIGVTKTTHIEDAVKASKLSLTAQEISDLEAV 304 Sbjct: 311 348 >AGc447 [C] COG0667 Predicted oxidoreductases (related to aryl-alcohol dehydrogenases) Length = 346 Score = 132 bits (331), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 0/278 (0%), Positives = 0/278 (0%), Gaps = 27/278 (9%) Query: 48 KAMEAGFNLWDTAAVYGIGASETILGNFTKGRKD-VLISTKFTPQI-----AG------D 95 + E + + + Sbjct: 77 GSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQR 136 Query: 96 S--ENAVEELLDGSLKRLGVDHADIYWIH--NP-ADVQRWTPKLIPLMKSGKVKYVGVSN 150 + + V D A R+ Sbjct: 137 PTNCF-GKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADK 195 Query: 151 HNLEEIKWAASIFAEEGLKISAVQNHYSLLYRSSEEAGILDYCKENGMIFFSYMVLEQGA 210 H+L I + + + L S E L G + L Sbjct: 196 HDLPRIVTIQNPY-----SLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAK 250 Query: 211 LTDKYNAQHPFPSDT-RRG-EAFNIDVFVKLEGLIQVMRDIGTKYNATTAQVAIAWSIAK 268 N + + + + VA A Sbjct: 251 PAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGAT 310 Query: 269 G--TVPIIGVTKTTHIEDAVKASKLSLTAQEISDLEAV 304 Sbjct: 311 TMD 348 >PA2535 [C] COG0667 Predicted oxidoreductases (related to aryl-alcohol dehydrogenases) Length = 346 Score = 132 bits (331), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 17/289 (5%), Positives = 35/289 (12%), Gaps = 25/289 (8%) Query: 36 YLTASDLK---PVFDKAMEAGFNLWDTAAVYGIGASETILGNFTKG-RKDVLISTKF--- 88 A + + K Sbjct: 27 NSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIAS 86 Query: 89 ----TPQIAG--DSENAVEELLDGSLKRLGVDHADIYWIH--NP-ADVQRWTPKLIPLMK 139 + + + + QR T L Sbjct: 87 KVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGY 146 Query: 140 SGKVKYVGVSNHNLEEIKWAASIFAEEGLKISAVQNHYSLLYRSSEEAGILDYCKENGMI 199 S VS + + + L + I Sbjct: 147 SWTDSAPAVSLLDTLDALAEYQRA-----GKIRYIGVSNETAFGVMRYLHLADKHDLPRI 201 Query: 200 FFSYMVLEQGALTDKYNAQHPFPSDTRR--GEAFNIDVFVKLEGLIQVMRDIGTKYNATT 257 + + + + + + L + Sbjct: 202 VTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSR 261 Query: 258 AQVAIAWSIAKGT--VPIIGVTKTTHIEDAVKASKLSLTAQEISDLEAV 304 K I A + L A Sbjct: 262 FTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGAT 310 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,534,372 Number of extensions: 951720 Number of successful extensions: 33416 Number of sequences better than 1.0e-05: 6316 Number of HSP's gapped: 8512 Number of HSP's successfully gapped: 6316 Length of query: 321 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 215 Effective length of database: 26,153,027 Effective search space: 5622900805 Effective search space used: 5622900805 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 116 (49.3 bits)