BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0032.p (258 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2083 [R] COG0300 Short-chain dehydrogenases of various substr... 198 8e-51 PA0658 [R] COG0300 Short-chain dehydrogenases of various substra... 140 2e-33 STM1724_1 [EH] COG0512 Anthranilate/para-aminobenzoate synthases... 134 1e-31 STM1724_2 [E] COG0547 Anthranilate phosphoribosyltransferase 134 1e-31 TM1724 [IQR] COG1028 Dehydrogenases with different specificities... 134 1e-31 >lin2083 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Length = 200 Score = 198 bits (503), Expect = 8e-51, Method: Compositional matrix adjust. Identities = 0/250 (0%), Positives = 0/250 (0%), Gaps = 5/250 (2%) Query: 3 LQDKVVVITGASSGIGALTAQMLGKRGAIPILLARSEDKLKDTAAGIPGVFGL----YAC 58 + I + ++ + + G Sbjct: 5 FGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRAATIELGPLVRGLPHLRTIHR 64 Query: 59 DVTDEEQVQNTFADILRVYGRIDVLLNNAGYGKFAAFTDMESREFDDMMDVNYMGIVRCT 118 + + + +Y + D + Y + M+ E + + Sbjct: 65 QLYQDIFDWAGQLREVDIY-QGDTPFCHFAYIEKEGNALMQDLEEEGYLVGLEKAKFVER 123 Query: 119 KAVVPHMLERGSGQIVNVASMAGKIGTARSVAYTATKHAVLGFTNALRQELRRQGIIVSA 178 A + V ++ A Q + + Sbjct: 124 LAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQLSWQGIEKEAWNQANQSGAMGDLT 183 Query: 179 VNPGPIATEFFRTADPSGSYEKSVARMMMTPEHVSKKIVKLIDKGKEEIDLPGFAAGGIR 238 Sbjct: 184 243 Query: 239 LYGLFPRLAD 248 Sbjct: 244 253 >PA0658 [R] COG0300 Short-chain dehydrogenases of various substrate specificities Length = 266 Score = 140 bits (354), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 89/249 (35%), Positives = 139/249 (55%), Gaps = 11/249 (4%) Query: 6 KVVVITGASSGIGALTAQMLGKRGAIPILLARSEDKLKDTAAGIPGVFGL----YACDVT 61 + +ITGASSGIG A+ L +RG IL+AR D L+ A + FG+ CD++ Sbjct: 3 RYALITGASSGIGLALAEALARRGRALILVARQRDALESIAYELAQRFGVEVLFRTCDLS 62 Query: 62 DEEQVQNTFADILRVYGRIDVLLNNAGYGKFAAFTDMESREFDDMMDVNYMGIVRCTKAV 121 + Q+ ++ + +ID+L+NNAG G F D E +++++N + + R ++ Sbjct: 63 EPLQLSGLLHELEQGERQIDLLVNNAGIGTSGPFIDQEWAREQELIELNILALARLCHSL 122 Query: 122 VPHMLERGSGQIVNVASMAGKIGTARSVAYTATKHAVLGFTNALRQELRRQGIIVSAVNP 181 M +G GQI+N+AS+A A Y A+K VL F+ LR+EL+ +G+ VS + P Sbjct: 123 GARMAAQGGGQILNIASVAAFQPGAWMSNYYASKAYVLHFSEGLREELKERGVKVSVLCP 182 Query: 182 GPIATEFFRTADPS-GSYEKSVARMMMTPEHVSKKIVKLIDKGKEEIDLPGFAAGGIRLY 240 GP T FFR A G ++S ++MMTPE V+ ++ +DK + I +PG+ RL Sbjct: 183 GPTRTAFFREAGMRLGRLDRS--KLMMTPEEVALLALRGLDKNRALI-VPGWRN---RLL 236 Query: 241 GLFPRLADK 249 L PRLA + Sbjct: 237 ALSPRLAPR 245 >STM1724_1 [EH] COG0512 Anthranilate/para-aminobenzoate synthases component II Length = 197 Score = 134 bits (337), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 0/226 (0%), Positives = 0/226 (0%), Gaps = 8/226 (3%) Query: 1 MALQDKVVVITGASSGIGALTAQMLGKRGAIPI---LLARSEDKLKDTAAGIPGVFGLYA 57 MA + I + + + + G Sbjct: 1 MADILLLDNIDSFTWNLADQLRTNGHNVVIYRNHIPAQTLIDRLATMKNPVLMLSPGPGV 60 Query: 58 CDVTDEEQVQNTFADILRVYGRIDVLLNNAGYGKFAAFTDMESREFDDMMDVNYMGIVRC 117 T I + + + G Sbjct: 61 PSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAGEILHGKASSIEHDGQAMF 120 Query: 118 TKAVVPHMLERGSGQIVNVASMAGKIGTARSVAYTATKHAVLGFTNALRQELRRQGIIVS 177 P + R + + I + A +H + Sbjct: 121 AGLANPLPVARYHSLVGSNVPAGLTINAHFNGMVMAVRHDADRVCGFQFHPESILTTQGA 180 Query: 178 AVNPGPIATEFFRTADPSGSYEKSVARMMM----TPEHVSKKIVKL 219 + +A + Sbjct: 181 RLLEQTLAWAQQKLE- 225 >STM1724_2 [E] COG0547 Anthranilate phosphoribosyltransferase Length = 334 Score = 134 bits (337), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 18/226 (7%), Positives = 45/226 (19%), Gaps = 8/226 (3%) Query: 1 MALQDKVVVITGASSGIGALTAQMLGKRGAIPI---LLARSEDKLKDTAAGIPGVFGLYA 57 LQ + + A + + Q+ + LA + +K + G Sbjct: 1 NTLQPILEKLYQAQTLTQQESHQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAAT 60 Query: 58 CDVTDEEQVQNTFADILRVYGRIDVLLNNAGYGKFAAFTDMESREFDDMMDVNYMGIVRC 117 + + + G N+ +AF + Sbjct: 61 ALLENAAPFPRPEYLFADIVGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSG 120 Query: 118 TKAVVPHMLERGSGQIVNVASMAGKIGTARSVAYTATKHAVLGFTNALRQELRRQGIIVS 177 + ++ ++G A + + R ++ Sbjct: 121 SSDLLAAFGINLDMNADKSRQALDELGVCFLFAPKYHTGLRHAMPVRQQLKTRTLFNVLG 180 Query: 178 AVNPGPIATEFFRTADPSGSYEKSVARMMM----TPEHVSKKIVKL 219 + S +A + V Sbjct: 181 PLINPAHPPLALIGV-YSPELVLPIAETLRVLGYQRAAVVHSGGMD 225 >TM1724 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 246 Score = 134 bits (337), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 81/226 (35%), Positives = 127/226 (56%), Gaps = 8/226 (3%) Query: 1 MALQDKVVVITGASSGIGALTAQMLGKRGAIPI---LLARSEDKLKDTAAGIPGVFGLYA 57 M L+ KV +ITGA+SGIG T + + GA I + + D L A G+PG Y Sbjct: 1 MRLEGKVCLITGAASGIGKATTLLFAQEGATVIAGDISKENLDSLVKEAEGLPGKVDPYV 60 Query: 58 CDVTDEEQVQNTFADILRVYGRIDVLLNNAGYGKFAAFTDMESREFDDMMDVNYMGIVRC 117 +VTD +Q++ +++ YGRIDVL+NNAG + A M+ ++D +++VN G+ Sbjct: 61 LNVTDRDQIKEVVEKVVQKYGRIDVLVNNAGITRDALLVRMKEEDWDAVINVNLKGVFNV 120 Query: 118 TKAVVPHMLERGSGQIVNVASMAGKIGTARSVAYTATKHAVLGFTNALRQELRRQGIIVS 177 T+ VVP+M+++ +G IVNV+S+ G G Y A+K V+G T +EL + I V+ Sbjct: 121 TQMVVPYMIKQRNGSIVNVSSVVGIYGNPGQTNYAASKAGVIGMTKTWAKELAGRNIRVN 180 Query: 178 AVNPGPIATEFFRTADPSGSYEKSVARMMM----TPEHVSKKIVKL 219 AV PG I T P + E +++R+ + PE V++ I+ L Sbjct: 181 AVAPGFIETPMTEKL-PEKARETALSRIPLGRFGKPEEVAQVILFL 225 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,250,889 Number of extensions: 745318 Number of successful extensions: 2404 Number of sequences better than 1.0e-05: 343 Number of HSP's gapped: 1992 Number of HSP's successfully gapped: 343 Length of query: 258 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 154 Effective length of database: 26,383,695 Effective search space: 4063089030 Effective search space used: 4063089030 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)