BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0028.p (270 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DR0470 [ER] COG1387 Histidinol phosphatase and related hydrolase... 138 1e-32 BS_ytvP [ER] COG1387 Histidinol phosphatase and related hydrolas... 86 6e-17 BH3206 [ER] COG1387 Histidinol phosphatase and related hydrolase... 62 9e-10 CAC2727 [ER] COG1387 Histidinol phosphatase and related hydrolas... 61 2e-09 lin0579 [ER] COG1387 Histidinol phosphatase and related hydrolas... 55 1e-07 >DR0470 [ER] COG1387 Histidinol phosphatase and related hydrolases of the PHP family Length = 346 Score = 138 bits (347), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 11/269 (4%), Positives = 32/269 (11%), Gaps = 26/269 (9%) Query: 4 DLHTHHFRCGHADGTIRDYIEAGIAAGLTVIGISDHTPYFGSESEQAFPRI-----AMAK 58 H+ GT+ + A + + I Sbjct: 6 IPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQG---------INLIDVAEMYPVP 56 Query: 59 SELARYVEEVLALQKEYAGTIDVLLGIESDYFPEYAELYRRTLSAYPFDYIIGSVHNVGG 118 + A I + + + + + Sbjct: 57 PRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHD 116 Query: 119 VSIFNK---SRWKNLDLQGKIASKAEYYRLITASARSGMFQILGHIDAMKGNYPQFSDI- 174 + + + + + S + Sbjct: 117 SLKRLQTDYLDLYQVHWPQRP---TNCFGKLGYSWTDSAPAVSLLDTLDA----LAEYQR 169 Query: 175 PATAEIDECLRVIGECGIAIEINTSGGTKLCGGWYPSDDILERALHYGVEVTFGSDAHVP 234 + + L V Sbjct: 170 AGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVE 229 Query: 235 SRVADQREAVAARLKEIGFTRWVYFKQTQ 263 K + + Sbjct: 230 LLAYSCLGFGTLTGKYLN-GAKPAGARNT 257 >BS_ytvP [ER] COG1387 Histidinol phosphatase and related hydrolases of the PHP family Length = 346 Score = 85.9 bits (211), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 13/266 (4%), Positives = 34/266 (12%), Gaps = 21/266 (7%) Query: 2 KFDLHTHHFRCGH-ADGTIRDYIEAGIAAGLTVIGISDHTPYFGSESEQA-FPRIAMAKS 59 + GT+ + A + + + + Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 60 ELARYVEEVLALQKEYAGTIDVLLGIESDYFPEYAE---LYRRTLSAYPFDYIIGSVHNV 116 L + + + + S+ + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHD-SLKRL 121 Query: 117 GGVSI-----FNKSRWKNL-DLQGKI-ASKAEYYRLITAS--ARSGMFQI--LGHIDAMK 165 + R N G A L+ + + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 166 GN---YPQFSDIPATAEIDECLRVIGECGIAIEINTSGGTKL-CGGWYPSDDILERALHY 221 + G Sbjct: 182 TAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTL 241 Query: 222 GVEVTFGSDAHVPSRVADQREAVAAR 247 + G+ R + Sbjct: 242 TGKYLNGAKPAGARNTLFSRFTRYSG 267 >BH3206 [ER] COG1387 Histidinol phosphatase and related hydrolases of the PHP family Length = 346 Score = 62.0 bits (149), Expect = 9e-10, Method: Compositional matrix adjust. Identities = 10/272 (3%), Positives = 34/272 (12%), Gaps = 29/272 (10%) Query: 2 KFDLHTHHFRCGHADGTIRD-YIEAGIAAGLTVIGISDHTPY-FGSESEQAFPRIAMAKS 59 + + A + + +E Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 60 ELARYVEEVLALQKEYAGTIDVLLGIESDYFPEYAELYRRTLSAYP--FDYIIGSVHNVG 117 L + + + + + + + S+ + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 118 GVSIFNKSRWKNLDLQGKIASKA------------EYYRLITASARSGM----FQILGH- 160 + + L+ +I Sbjct: 123 -----TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIG 177 Query: 161 --IDAMKGNYPQFSDIPATAEIDECLRVIGECGIAIEINTSGGTK-LCGGWYPSDDILER 217 + G + G Sbjct: 178 VSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLG 237 Query: 218 ALHYGVEVTFGSDAHVPSRVADQREAVAARLK 249 + G+ R + + Sbjct: 238 FGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQ 269 >CAC2727 [ER] COG1387 Histidinol phosphatase and related hydrolases of the PHP family Length = 346 Score = 60.8 bits (146), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 13/250 (5%), Positives = 36/250 (14%), Gaps = 37/250 (14%) Query: 1 MKFDLHTHHFRCGHADGTIRDYIEAGIAAGLTVIGISDHTPYFGSESEQAFPRIAMAKSE 60 M++ H G + D+ + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHA-QLDYA-------VAQGI----NLID 48 Query: 61 LARYVEEVLALQKEYAGTIDVLLGIESDYFPEYAELYRRTLSAYPFDYIIGSVHNVGGVS 120 +A + + + + + + +S + G + Sbjct: 49 VAEMYPVPPRPETQGLTE-TYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDR 107 Query: 121 IFNKSRWKNLDLQGKIASKAEYYRLITASARSGMF-QILGHIDAMKG---------NYPQ 170 + + K + Sbjct: 108 KNIREALHD---SLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDAL 164 Query: 171 FSDIPATAEIDECLRVIGECGIAIEINTSGGTK--LCGGWYPSDDILERALHYGVE-VTF 227 +R IG + + + + Sbjct: 165 AEYQ-----RAGKIRYIGVSNETAFGVMRYLHLADKHDLPRI---VTIQNPYSLLNRSFE 216 Query: 228 GSDAHVPSRV 237 A V Sbjct: 217 VGLAEVSQYE 226 >lin0579 [ER] COG1387 Histidinol phosphatase and related hydrolases of the PHP family Length = 346 Score = 55.5 bits (132), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 13/262 (4%), Positives = 37/262 (14%), Gaps = 32/262 (12%) Query: 1 MKFDLHTHHFRCGHAD-GTIRDYIEAGIAAGLTVIGISDHTPYFGSE-------SEQAFP 52 M++ H + E A + P Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQ-GINLIDVAEMYPVPPRP 59 Query: 53 RIAMAKSELARYVEEVLALQKEYAGTIDVLLGIESDYFPEYAELYRRTLSAY--PFDYII 110 +++ V ++ + + D + Sbjct: 60 ETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLK 119 Query: 111 GSVHNVGGVSIFNKSRWKNLDL------------QGKIASKAEYYRLITASARSGMFQIL 158 + + + + + + + + + Sbjct: 120 RLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVS 179 Query: 159 GHIDAMKGNYPQF-----SDIPA--TAEIDECLRVIGECGIAIEINTSGGTK-LCGGWYP 210 A L E G+A G Sbjct: 180 NET-AFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGF 238 Query: 211 SDDILERALHYGVEVTFGSDAH 232 + + Sbjct: 239 GTLTGKYLNGAKPAGARNTLFS 260 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.138 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,181,802 Number of extensions: 898263 Number of successful extensions: 1549 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 1540 Number of HSP's successfully gapped: 5 Length of query: 270 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 165 Effective length of database: 26,268,361 Effective search space: 4334279565 Effective search space used: 4334279565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)