BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0027.p (280 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2953 [C] COG0778 Nitroreductase 272 3e-73 BS_ycnD [C] COG0778 Nitroreductase 232 6e-61 CAC0718 [C] COG0778 Nitroreductase 226 3e-59 lin2216 [C] COG0778 Nitroreductase 226 4e-59 BH1048 [C] COG0778 Nitroreductase 170 3e-42 >BH2953 [C] COG0778 Nitroreductase Length = 346 Score = 272 bits (696), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 12/250 (4%), Positives = 35/250 (14%), Gaps = 3/250 (1%) Query: 33 LNEVIRTLMNHRSFRQYRQEPVEPEKLKTIIKAAQAAPSWVNGQQVSVIAVRSEERRQRL 92 + + E+ AQ + G + +A + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 93 SVLSGNQKHVAQAPVFLVFCMDFYRAKVAGELEGQPFEAAKDVDALLVGATDVGIALSNA 152 + +KV+G D + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 153 IAAAESLGLGIIPIGGVRRHTAEVIELLQLPEYVFPVVGLCVGYPAEELPKKPRLPLEAV 212 + + ++ ++ + K R + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 213 LHEEQYNPEVKSLIEAYNLAHRASLKEQ--GLTERDWSSTIAHFYTLNPQYGDAQHTLKR 270 + + + Y + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYE-GVELLAYSCLGFG 239 Query: 271 QGFTCDNLKD 280 Sbjct: 240 TLTGKYLNGA 249 >BS_ycnD [C] COG0778 Nitroreductase Length = 346 Score = 232 bits (591), Expect = 6e-61, Method: Compositional matrix adjust. Identities = 17/249 (6%), Positives = 38/249 (15%), Gaps = 6/249 (2%) Query: 33 LNEVIRTLMNHRSFRQYRQEPVEPEKLKTIIKAAQAAPSWVNGQQVSVIAVRSEERRQRL 92 + + E+ AQ + G + +A + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 93 SVLSGNQKHVAQAPVFLVFCMDFYRAKVAGE-LEGQPFEAAKDVDALLVGATDVGIALSN 151 + +KV+G + +L Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 152 AIAAAESLGLGIIPIGGVRRHTAEVIELLQLPEYVFPVVGLCVGYPAEELPKKPRLPLEA 211 L P V + L + K + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 212 VLHEEQY--NPEVKSLIEAYN--LAHRASLKEQGLTERDWSSTIAHFYTLNPQYGDAQHT 267 + + SL + + Y Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVE-LLAYSCLGFG 239 Query: 268 LKRQGFTCD 276 + Sbjct: 240 TLTGKYLNG 248 >CAC0718 [C] COG0778 Nitroreductase Length = 346 Score = 226 bits (576), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 12/246 (4%), Positives = 37/246 (15%), Gaps = 4/246 (1%) Query: 33 LNEVIRTLMNHRSFRQYRQEPVEPEKLKTIIKAAQAAPSWVNGQQVSVIAVRSEERRQRL 92 + + E+ AQ + G + +A + Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 93 SVLSGNQKHVAQAPVFLVFCMDFYRAKVAGELEGQPFEAAKDVDALLVGATDVGIALSNA 152 + +KV+G D + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 153 IAAAESLGLGIIPIGGVRRHTAEVIELLQLPEYVFPVVGLCVGYPAEELPKKPRLPLEAV 212 + + ++ ++ + K R + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 213 LHEEQYNPE-VKSLIEAYNLAHRASLKEQGLTER--DWSSTIAHFYTLNPQYGDAQHTLK 269 + L + ++ + + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEG-VELLAYSCLGFG 239 Query: 270 RQGFTC 275 Sbjct: 240 TLTGKY 245 >lin2216 [C] COG0778 Nitroreductase Length = 346 Score = 226 bits (575), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 13/242 (5%), Positives = 30/242 (12%), Gaps = 4/242 (1%) Query: 33 LNEVIRTLMNHRSFRQYRQEPVEPE-KLKTIIKAAQAAPSWVNGQQVSVIAVRSEERRQR 91 + + E + A + V + R Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 92 LSVLSGNQKHVAQAPVFLVFCMDFYRAKVAGELEGQPFEAAKDVDALLVGATDVGIALSN 151 L+ A + + +L Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 152 AIAAAESLGLGIIPIGGVRRHTAEVIELLQLPEYVFPVVGLCVGYPAEELPKKPRLPLEA 211 L P V + L + K + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 212 VLHEEQYNPEVKSLIEAYNLAHRASLKEQGLTERDWSSTIAHFYTLNPQYGDAQHTLKRQ 271 + L + + + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLN--RSFEVGLAEVS-QYEGVELLAYSCLG 237 Query: 272 GF 273 Sbjct: 238 FG 239 >BH1048 [C] COG0778 Nitroreductase Length = 346 Score = 170 bits (430), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 13/226 (5%), Positives = 32/226 (14%), Gaps = 5/226 (2%) Query: 34 NEVIRTLMNHRSFRQYRQEPVEPEKLKTIIKAAQAAPSWVNGQQVSVIAVRSEERRQRLS 93 I S + + A + V + R Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 94 VLSGNQKHVAQAPVFLVFCMDFYRAKVAGELEGQPFEAAKDVDALLVGATDVGIALSNAI 153 L+ A + D + + Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIR-PDQALDRKNIREALHDSLKRL 121 Query: 154 AAAESLGLGIIPIGGVRRHTAEVIELLQLPEYVFPVVGLCVGYPAEELPKKPRLPLEAVL 213 + ++ ++ + K R + Sbjct: 122 QTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNE 181 Query: 214 HEEQYNPEVKSLIEAYNLAHRA--SLKEQGLTERDWSSTIA-HFYT 256 + + ++L L Y Sbjct: 182 TAFGVMRYLHLA-DKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYE 226 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,906,823 Number of extensions: 906433 Number of successful extensions: 1962 Number of sequences better than 1.0e-05: 42 Number of HSP's gapped: 1926 Number of HSP's successfully gapped: 42 Length of query: 280 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 175 Effective length of database: 26,268,361 Effective search space: 4596963175 Effective search space used: 4596963175 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)