BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0015.p (453 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yubG [P] COG0168 Trk-type K+ transport systems, membrane comp... 390 e-108 BH0598 [P] COG0168 Trk-type K+ transport systems, membrane compo... 378 e-105 DR1668 [P] COG0168 Trk-type K+ transport systems, membrane compo... 340 3e-93 all1802 [P] COG0168 Trk-type K+ transport systems, membrane comp... 288 1e-77 FN1725 [P] COG0168 Trk-type K+ transport systems, membrane compo... 287 3e-77 >BS_yubG [P] COG0168 Trk-type K+ transport systems, membrane components Length = 445 Score = 390 bits (1001), Expect = e-108, Method: Compositional matrix adjust. Identities = 204/449 (45%), Positives = 304/449 (67%), Gaps = 6/449 (1%) Query: 5 LPKINNFRFLKLSPPQILVLGFAAVILIGTLLLMLPVSSTSGESLNFIDALFTATSATCV 64 L K ++++ +PPQ+L +GF I+IG +LLMLP+S+T + L++IDALFTA SAT V Sbjct: 3 LQKDKVIKWVRFTPPQVLAIGFFLTIIIGAVLLMLPISTT--KPLSWIDALFTAASATTV 60 Query: 65 TGLVVVDTGTYFTVFGQTVIMVLIQVGGLGFMTMATLFALVLKRRISLRDRLILQEAMNQ 124 TGL VVDTGT FTVFGQTVIM LIQ+GGLGFMT A L ++L ++I L++R+++QEA+NQ Sbjct: 61 TGLAVVDTGTQFTVFGQTVIMGLIQIGGLGFMTFAVLIVMILGKKIGLKERMLVQEALNQ 120 Query: 125 SSMEGIVRLIRKVLIYSLVIEAGAAVILSMRWAFDMPFGRAVYFGIFHAVSMFNNAGFDI 184 ++ G++ L++ + ++S+ IE AA+ILS+R + ++ +FHA+S FNNAGF + Sbjct: 121 PTIGGVIGLVKVLFLFSISIELIAALILSIRLVPQYGWSSGLFASLFHAISAFNNAGFSL 180 Query: 185 FGDYRSLTGYVSDPIVNIVVMFLIVSGGIGFIVMSDLVDYRRRTRRLSLHSKVVLSMTAA 244 + D +L YV DP VN+V+ FL ++GGIGF V+ D++ RR + SLH+K++L+ T Sbjct: 181 WPD--NLMSYVGDPTVNLVITFLFITGGIGFTVLFDVMK-NRRFKTFSLHTKLMLTGTLM 237 Query: 245 LIVIGTVVIFVFEFTNTRTLGPLNFGGKIWAAFFQSVTPRTAGANTVDIAGLRQASQFFI 304 L I + +F+ E++N TLG L+ K+WA++FQ+VTPRTAG N++D +R+ + F Sbjct: 238 LNAIAMLTVFILEYSNPGTLGHLHIVDKLWASYFQAVTPRTAGFNSLDFGSMREGTIVFT 297 Query: 305 VILMFIGASPGSTGGGIKTTTFTLMIGAVISMLRGREDIVLFRYRLAQERVFKXXXXXXX 364 ++LMFIGA ST GIK TTF +++ +VI+ LRG+++ V+FR + + K Sbjct: 298 LLLMFIGAGSASTASGIKLTTFIVILTSVIAYLRGKKETVIFRRSIKYPIIIKALAVSVT 357 Query: 365 XXXXXXXXSMALSTTEDRAFLMILFETTSAFATVGLSMGLTPELSQAGKILICLTMFAGR 424 AL+ TE FL I+FET SAF TVGL+MGLTPEL+ AGK +I + MF GR Sbjct: 358 SLFIVFLGIFALTITEQAPFLQIVFETFSAFGTVGLTMGLTPELTTAGKCIIIVIMFIGR 417 Query: 425 LGLLTLAYAIGPKQGKQLYKYPEGKMIIG 453 +G LT ++ + + +YP+G++ G Sbjct: 418 IGPLTFVFSFAKTEQSNI-RYPDGEVFTG 445 >BH0598 [P] COG0168 Trk-type K+ transport systems, membrane components Length = 448 Score = 378 bits (971), Expect = e-105, Method: Compositional matrix adjust. Identities = 210/443 (47%), Positives = 297/443 (67%), Gaps = 8/443 (1%) Query: 12 RFLKLSPPQILVLGFAAVILIGTLLLMLPVSSTSGESLNFIDALFTATSATCVTGLVVVD 71 + + LSPPQIL L F I+IGTLLL LP++ST+ ++++DA+FTATSA VTGLVVVD Sbjct: 13 KVIHLSPPQILALSFLVFIMIGTLLLKLPIASTT--YISWVDAMFTATSAITVTGLVVVD 70 Query: 72 TGTYFTVFGQTVIMVLIQVGGLGFMTMATLFALVLKRRISLRDRLILQEAMNQSSMEGIV 131 TGT +TVFGQ VIM+LIQ+GGLG MT A L ++L ++I L+ R+++QEAMNQ+S+ G++ Sbjct: 71 TGTDYTVFGQVVIMILIQLGGLGLMTFAVLAVMMLGKKIGLQQRILIQEAMNQTSLGGLI 130 Query: 132 RLIRKVLIYSLVIEAGAAVILSMRWAFDMPFGRAVYFGIFHAVSMFNNAGFDIFGDYRSL 191 RL+R +L+++LV+E A ++LS+RW + + +Y +FH +S FNNAGF ++ D SL Sbjct: 131 RLVRVLLLFTLVMETIAFILLSIRWVPEFGWREGMYQSLFHTISAFNNAGFSLWSD--SL 188 Query: 192 TGYVSDPIVNIVVMFLIVSGGIGFIVMSDLVDYRRRTRRLSLHSKVVLSMTAALIVIGTV 251 + YV DP +N+++ L ++GGIGF V++DL Y++ R LSLHSK++L T AL I + Sbjct: 189 SAYVGDPAINLIITILFITGGIGFTVLADLW-YKKTFRALSLHSKIMLLGTLALNCIAFI 247 Query: 252 VIFVFEFTNTRTLGPLNFGGKIWAAFFQSVTPRTAGANTVDIAGLRQASQFFIVILMFIG 311 IF+ E+ N TLG L + W A+FQ+VTPRTAG NT++IA + AS I++LMFIG Sbjct: 248 AIFILEYANPHTLGGLAQSDRFWGAYFQAVTPRTAGFNTLEIADMTTASIVLILLLMFIG 307 Query: 312 ASPGSTGGGIKTTTFTLMIGAVISMLRGREDIVLFRYRLAQERVFKXXXXXXXXXXXXXX 371 A ST GIK TTF +MI A S L+GR + V+F + + + Sbjct: 308 AGSASTASGIKVTTFFVMILATWSFLKGRAETVVFERTIKLHTILRALAIIVISMMIVFI 367 Query: 372 XSMALSTTEDRAFLMILFETTSAFATVGLSMGLTPELSQAGKILICLTMFAGRLGLLTLA 431 L+ TED F++ +FE SAF TVGLSMGLT EL+ GK+ I + MF GR+G LT+A Sbjct: 368 AVFILAITEDAPFIVKVFEVFSAFGTVGLSMGLTGELTTLGKMTIMVVMFIGRVGPLTMA 427 Query: 432 YAIG-PKQGKQLYKYPEGKMIIG 453 + + PKQ +YPE ++ G Sbjct: 428 FTLARPKQSN--IRYPEDEVFTG 448 >DR1668 [P] COG0168 Trk-type K+ transport systems, membrane components Length = 512 Score = 340 bits (872), Expect = 3e-93, Method: Compositional matrix adjust. Identities = 195/444 (43%), Positives = 282/444 (63%), Gaps = 8/444 (1%) Query: 15 KLSPPQILVLGFAAVILIGTLLLMLPVSSTSGESLNFIDALFTATSATCVTGLVVVDTGT 74 +L PPQ++ L FAA ILIG +LL LP + +G+S+ FI ALF ATS CVTGL VVD G+ Sbjct: 69 RLRPPQLIALTFAAAILIGAVLLWLPFAHEAGKSITFIQALFMATSCVCVTGLAVVDPGS 128 Query: 75 YFTVFGQTVIMVLIQVGGLGFMTMATLFALVLKRRISLRDRLILQEAMNQSSMEGIVRLI 134 F VFG++V+++LIQVGGLG +T+ TLFAL L+RR+ + R+ Q+ ++ +S+ L+ Sbjct: 129 TFNVFGESVMLLLIQVGGLGIITLGTLFALALRRRLGVIGRINAQQQVSATSLGSATSLV 188 Query: 135 RKVLIYSLVIEAGAAVILSMRWAFDMPFGRAVYFGIFHAVSMFNNAGFDIFGDYRSLTGY 194 R +++ SL+ E A++L + R +Y+ IFH++S FNNAGF ++ +LTG+ Sbjct: 189 RSIVLTSLLFEGLGALLLLPAFIPREGVLRGIYYAIFHSISSFNNAGFSLYST--NLTGF 246 Query: 195 VSDPIVNIVVMFLIVSGGIGFIVMSDLVDY--RRRTRRLSLHSKVVLSMTAALIVIGTVV 252 VSDP++N+VV LI+ GG+GF+V +L+ + RR RLS ++++ L+MT L+V+GTV Sbjct: 247 VSDPLINMVVTLLIIFGGMGFLVQLNLLGWLRDRRRNRLSTNTRLSLTMTGVLLVVGTVF 306 Query: 253 IFVFEFTNTRTLGPLNFGGKIWAAFFQSVTPRTAGANTVDIAGLRQASQFFIVILMFIGA 312 + E+ N TL PL GK+ A++FQSVTPRTAG NT+D + + F ++ MFIGA Sbjct: 307 FALTEWNNPGTLAPLPLHGKLLASWFQSVTPRTAGFNTLDYGLMGFPALFMTIVWMFIGA 366 Query: 313 SPGSTGGGIKTTTFTLMIGAVISMLRGREDIVLFRYRLAQERVFKXXXXXXXXXXXXXXX 372 +PGSTGGGIKT TF +++ + SM+RGR D LF R+ E + + Sbjct: 367 NPGSTGGGIKTNTFYVIVASAWSMVRGRRDTTLFDRRIDTETLLRAMTVGLLSMGLVTAG 426 Query: 373 SM---ALSTTEDRAFLMILFETTSAFATVGLSMGLTPELSQAGKILICLTMFAGRLGLLT 429 M L+T + FL + FET SAF T GLSM TP L+ A + ++ MF GR+G LT Sbjct: 427 FMLMLLLNTNKKVLFLQLFFETVSAFGTAGLSMNTTPLLNTAQEAVLIALMFLGRIGPLT 486 Query: 430 LAYAIG-PKQGKQLYKYPEGKMII 452 A A P K L +YP + I+ Sbjct: 487 FAVAFSRPASEKMLTRYPADRDIL 510 >all1802 [P] COG0168 Trk-type K+ transport systems, membrane components Length = 444 Score = 288 bits (738), Expect = 1e-77, Method: Compositional matrix adjust. Identities = 174/440 (39%), Positives = 266/440 (60%), Gaps = 10/440 (2%) Query: 22 LVLGFAAVILIGTLLLMLPVSSTSGESLNFIDALFTATSATCVTGLVVVDTGTYFTVFGQ 81 + LGF AVI++GT+LLM+P S+++G + I A+FT+TSA CVTGL VVDTGTYF+ +GQ Sbjct: 7 VCLGFIAVIVVGTILLMMPFSTSNGNWNDPIVAIFTSTSAVCVTGLAVVDTGTYFSFWGQ 66 Query: 82 TVIMVLIQVGGLGFMTMATLFALVLKRRISLRDRLILQEAMNQSSMEGIVRLIRKVLIYS 141 I +L Q+GGLG+MT T L++ R+ LR ++ +Q+A+++ M G ++IR ++ + Sbjct: 67 FFIALLAQIGGLGYMTTTTFLILLIGRKFDLRQKIAIQQALDRPGMSGSTQVIRSIIATT 126 Query: 142 LVIEAGAAVILSMRWAFDMPFGRAVYFGIFHAVSMFNNAGFDIFGDYRSLTGYVSDPIVN 201 L+ E +L + D + + ++ IFH+V+ +NNAGF +F D +L Y S +V Sbjct: 127 LIFEITGVFLLLPAFVPDYGWNQGLWLAIFHSVNAWNNAGFSLFKD--NLISYQSSSLVV 184 Query: 202 IVVMFLIVSGGIG-------FIVMSDLVDYRRRTRRLSLHSKVVLSMTAALIVIGTVVIF 254 + LI+ GGIG +I + D + ++ T SL KV S T L+ +GT+ F Sbjct: 185 FTITGLIIFGGIGYQVILETYIWLRDRILKKKETLVFSLDFKVATSTTLILLFLGTIAFF 244 Query: 255 VFEFTNTRTLGPLNFGGKIWAAFFQSVTPRTAGANTVDIAGLRQASQFFIVILMFIGASP 314 E N +T G LN + A+FQSVTPRTAG NT+DI + A F + LMFIGASP Sbjct: 245 CIEVRNPQTFGSLNLRDQFLVAWFQSVTPRTAGFNTIDIGKMTTAGLFITIALMFIGASP 304 Query: 315 GSTGGGIKTTTFTLMIGAVISMLRGREDIVLFRYRLAQERVFKXXXXXXXXXXXXXXXSM 374 G TGGG+KTTT ++ ++L+G+E+++L+ ++A + K ++ Sbjct: 305 GGTGGGMKTTTLRVLTSCTKTILQGKEEVLLYERKIAVALILKAVGVLVGSIATVILATI 364 Query: 375 ALSTTEDR-AFLMILFETTSAFATVGLSMGLTPELSQAGKILICLTMFAGRLGLLTLAYA 433 +S T+ + F+ ILFE SAFATVGLS G+T +S A K+++ +TM+ GR+G+L L A Sbjct: 365 LISLTDPKLDFIQILFEVVSAFATVGLSTGITASVSPAAKLVLIITMYVGRVGVLLLMSA 424 Query: 434 IGPKQGKQLYKYPEGKMIIG 453 I YPE +++G Sbjct: 425 ILGDPRPTRIHYPEENLLVG 444 >FN1725 [P] COG0168 Trk-type K+ transport systems, membrane components Length = 448 Score = 287 bits (734), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 175/443 (39%), Positives = 267/443 (60%), Gaps = 4/443 (0%) Query: 12 RFLKLSPPQILVLGFAAVILIGTLLLMLPVSSTSGESLNFIDALFTATSATCVTGLVVVD 71 ++ LSP + L+ GF I IG +LL +P S +++ +D+LFT SA CVTGL VVD Sbjct: 9 KWDSLSPYRKLIFGFLVAIFIGVILLKMPFSLRENQNITVLDSLFTIVSAICVTGLSVVD 68 Query: 72 TGTYFTVFGQTVIMVLIQVGGLGFMTMATLFALVLKRRISLRDRLILQEAMNQSSMEGIV 131 FT GQ +I+ IQ+GGLG MT++ + L++ +++S R +L+E N +S GI Sbjct: 69 ISQVFTSTGQLIILFFIQLGGLGVMTVSIIVFLLVGKKMSFETRELLKEERNSNSNGGIT 128 Query: 132 RLIRKVLIYSLVIEAGAAVILSMRWAFDMPFGRAVYFGIFHAVSMFNNAGFDIFGDYRSL 191 I+ +L+ +IE A IL+ ++ P +++++G+FH+VS F NAGF +F + +L Sbjct: 129 NFIKNLLLTVSLIEILGASILAYGFSRYYPLKKSIFYGLFHSVSAFCNAGFSLFTN--NL 186 Query: 192 TGYVSDPIVNIVVMFLIVSGGIGFIVMSDL-VDYRRRTRRLSLHSKVVLSMTAALIVIGT 250 + D ++++ + FLI+ GGIGF+ ++ L + +++ + LSL SK L +T L+ GT Sbjct: 187 EDFRYDKLISLTISFLIILGGIGFVTVNSLFIIKKKKLKNLSLTSKFALLITFFLLTFGT 246 Query: 251 VVIFVFEFTNTRTLGPLNFGGKIWAAFFQSVTPRTAGANTVDIAGLRQASQFFIVILMFI 310 ++ VFE+ N+ TL +NF KI +FFQSVT RTAG NTV + + A+ F I MFI Sbjct: 247 MLFLVFEYNNSSTLKGMNFVDKIINSFFQSVTLRTAGFNTVPLENIGPATVFISYIFMFI 306 Query: 311 GASPGSTGGGIKTTTFTLMIGAVISMLRGREDIVLFRYRLAQERVFKXXXXXXXXXXXXX 370 GASPGSTGGGIKTTTF ++I +L+ +E + +F+ R+ E + K Sbjct: 307 GASPGSTGGGIKTTTFGILIFYAFGVLKRKEYVEVFKRRIDWELINKALAIVIISLFYII 366 Query: 371 XXSMALSTTEDRAFLMILFETTSAFATVGLSMGLTPELSQAGKILICLTMFAGRLGLLTL 430 L + E + +++E SAF+T GLSMG+T L K LI +TMF GRLG +T+ Sbjct: 367 VVITILLSIESFSADKVIYEVISAFSTTGLSMGITASLGIISKFLIIITMFIGRLGPMTV 426 Query: 431 AYAIGPKQGKQLYKYPEGKMIIG 453 A A + K L KYP+ ++IG Sbjct: 427 ALAFTNNK-KSLVKYPKEDILIG 448 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.143 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,466,965 Number of extensions: 1059487 Number of successful extensions: 2967 Number of sequences better than 1.0e-05: 18 Number of HSP's gapped: 2901 Number of HSP's successfully gapped: 18 Length of query: 453 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 343 Effective length of database: 25,691,691 Effective search space: 8812250013 Effective search space used: 8812250013 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 117 (49.7 bits)