BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0011.p (272 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TM1009 [R] COG0656 Aldo/keto reductases, related to diketogulona... 328 7e-90 YPO2805 [R] COG0656 Aldo/keto reductases, related to diketogulon... 313 2e-85 lin2739 [R] COG0656 Aldo/keto reductases, related to diketogulon... 293 2e-79 SA2001 [R] COG0656 Aldo/keto reductases, related to diketogulona... 292 3e-79 STM1676 [R] COG0656 Aldo/keto reductases, related to diketogulon... 239 3e-63 >TM1009 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 328 bits (840), Expect = 7e-90, Method: Compositional matrix adjust. Identities = 15/273 (5%), Positives = 46/273 (16%), Gaps = 5/273 (1%) Query: 1 MPIIGFGVYQVPDAEECENAVYEALMAGYRLIDTAAGYLNEEAVGRAIKRSG----VPRE 56 + + +A + A + I+ R + V Sbjct: 14 STLGLGTMTFGE-QNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNW 72 Query: 57 ELFITTKLWVQDAGYESXXXXXXXXXXXXXXXXXXXXXIHQPFGDYYGAWRAMEDLYREG 116 ++ + A S DLY+ Sbjct: 73 LAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVH 132 Query: 117 KIKAIGVSNFLPDRLMDLIVHNEIVPAVNQIETHPFYQQTESAAFMKEQGVQHQSWAPFA 176 + + + + Sbjct: 133 WPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHL 192 Query: 177 EGRNNMFGNEVLASIAEKYNKSIAQVILRWLVQREVVVIPKSVRKERIVENFNIFDFELS 236 ++++ + + N+S + V ++ S + + + + Sbjct: 193 ADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPA 252 Query: 237 ADDIEQISALDTRESLFLSYHDPKFAKMLGTLR 269 S + + Sbjct: 253 GARNTLFSRFTRYSGEQTQKAVAAYVDIARRHG 285 >YPO2805 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 313 bits (803), Expect = 2e-85, Method: Compositional matrix adjust. Identities = 14/272 (5%), Positives = 38/272 (13%) Query: 1 MPIIGFGVYQVPDAEECENAVYEALMAGYRLIDTAAGYLNEEAVGRAIKRSGVPREELFI 60 Q+ A + + L E VG + + G + + Sbjct: 26 QNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIA 85 Query: 61 TTKLWVQDAGYESXXXXXXXXXXXXXXXXXXXXXIHQPFGDYYGAWRAMEDLYREGKIKA 120 + + Q + Sbjct: 86 SKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLG 145 Query: 121 IGVSNFLPDRLMDLIVHNEIVPAVNQIETHPFYQQTESAAFMKEQGVQHQSWAPFAEGRN 180 ++ P + + + + M+ + + P Sbjct: 146 YSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQ 205 Query: 181 NMFGNEVLASIAEKYNKSIAQVILRWLVQREVVVIPKSVRKERIVENFNIFDFELSADDI 240 N + + S + + Sbjct: 206 NPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRY 265 Query: 241 EQISALDTRESLFLSYHDPKFAKMLGTLRVDL 272 + L Sbjct: 266 SGEQTQKAVAAYVDIARRHGLDPAQMALAFVR 297 >lin2739 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 293 bits (749), Expect = 2e-79, Method: Compositional matrix adjust. Identities = 15/272 (5%), Positives = 47/272 (17%) Query: 1 MPIIGFGVYQVPDAEECENAVYEALMAGYRLIDTAAGYLNEEAVGRAIKRSGVPREELFI 60 + E + L V + G+ + Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGN 71 Query: 61 TTKLWVQDAGYESXXXXXXXXXXXXXXXXXXXXXIHQPFGDYYGAWRAMEDLYREGKIKA 120 + + + Sbjct: 72 WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQV 131 Query: 121 IGVSNFLPDRLMDLIVHNEIVPAVNQIETHPFYQQTESAAFMKEQGVQHQSWAPFAEGRN 180 + PAV+ ++T + + A ++ GV +++ + Sbjct: 132 HWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLH 191 Query: 181 NMFGNEVLASIAEKYNKSIAQVILRWLVQREVVVIPKSVRKERIVENFNIFDFELSADDI 240 +++ + + S+ + + + + L+ Sbjct: 192 LADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKP 251 Query: 241 EQISALDTRESLFLSYHDPKFAKMLGTLRVDL 272 S + A Sbjct: 252 AGARNTLFSRFTRYSGEQTQKAVAAYVDIARR 283 >SA2001 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 292 bits (748), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 14/272 (5%), Positives = 33/272 (12%), Gaps = 1/272 (0%) Query: 1 MPIIGFGVYQVPDAEECENAVYEALMAGYRLIDTAAGYLNEEAVGRAIKRSGVPREELFI 60 + E A A + + E + E Sbjct: 12 EVSTLGLGTMTFGEQNSE-ADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVG 70 Query: 61 TTKLWVQDAGYESXXXXXXXXXXXXXXXXXXXXXIHQPFGDYYGAWRAMEDLYREGKIKA 120 + + + Sbjct: 71 NWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQ 130 Query: 121 IGVSNFLPDRLMDLIVHNEIVPAVNQIETHPFYQQTESAAFMKEQGVQHQSWAPFAEGRN 180 + + L + A A Sbjct: 131 VHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYL 190 Query: 181 NMFGNEVLASIAEKYNKSIAQVILRWLVQREVVVIPKSVRKERIVENFNIFDFELSADDI 240 ++ L I N + EV F + Sbjct: 191 HLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAK 250 Query: 241 EQISALDTRESLFLSYHDPKFAKMLGTLRVDL 272 + + + + + Sbjct: 251 PAGARNTLFSRFTRYSGEQTQKAVAAYVDIAR 282 >STM1676 [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 239 bits (610), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 14/252 (5%), Positives = 37/252 (14%), Gaps = 5/252 (1%) Query: 1 MPIIGFGVYQVPDAEECENAVYEALMAGYRLIDTAAGYLNEEAVGRAIKR---SGV-PRE 56 + E + L V + + Sbjct: 12 EVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGN 71 Query: 57 ELFITTKLWVQD-AGYESXXXXXXXXXXXXXXXXXXXXXIHQPFGDYYGAWRAMEDLYRE 115 L A S DLY+ Sbjct: 72 WLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQV 131 Query: 116 GKIKAIGVSNFLPDRLMDLIVHNEIVPAVNQIETHPFYQQTESAAFMKEQGVQHQSWAPF 175 + + + + Sbjct: 132 HWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLH 191 Query: 176 AEGRNNMFGNEVLASIAEKYNKSIAQVILRWLVQREVVVIPKSVRKERIVENFNIFDFEL 235 ++++ + + N+S + V ++ S + + + Sbjct: 192 LADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKP 251 Query: 236 SADDIEQISALD 247 + S Sbjct: 252 AGARNTLFSRFT 263 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,252,846 Number of extensions: 730033 Number of successful extensions: 7655 Number of sequences better than 1.0e-05: 80 Number of HSP's gapped: 7528 Number of HSP's successfully gapped: 87 Length of query: 272 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 167 Effective length of database: 26,268,361 Effective search space: 4386816287 Effective search space used: 4386816287 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)