BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0010.p (337 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_uvrC [L] COG0322 Nuclease subunit of the excinuclease complex 343 3e-94 lin1197 [L] COG0322 Nuclease subunit of the excinuclease complex 342 5e-94 ML0258 [FP] COG0248 Exopolyphosphatase 271 2e-72 CAC0508 [L] COG0322 Nuclease subunit of the excinuclease complex 206 3e-53 Rv1420 [L] COG0322 Nuclease subunit of the excinuclease complex 164 2e-40 >BS_uvrC [L] COG0322 Nuclease subunit of the excinuclease complex Length = 588 Score = 343 bits (879), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 14/256 (5%), Positives = 44/256 (17%) Query: 59 LQEWLGVKVLVPQRGLKKQMVGMACQNGRVALDEKFRLIERDEERTSGAAIXXXXXXXXX 118 + + K + K A L ++ + +R S + Sbjct: 317 RKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEVKRMECF 376 Query: 119 XXXRIEAFDNSNIQGTNPVSAMVVFIDGKPARKEYRKYKVRSVQGPDDYETMREVIRRRY 178 + + + + + I Sbjct: 377 DISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRYGKAIDDSK 436 Query: 179 ERVLKENLPQPDLIVVDGGKGQISSAIDILQNELGLYIPVCGLVKDDKHRTAQLLVGDSA 238 + + + L L + K G+ Sbjct: 437 IPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLETLFFEPEGEGF 496 Query: 239 EPVQLARDSQEFYLLQRIQDEVHRFAITFHREQRGKSMVTSKLDSIPGIGDKRRKLLLKH 298 + ++ + R + + ++ + + + Sbjct: 497 SLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGPKRRQMLLKYMGG 556 Query: 299 FGSLKKIKEASIEDFR 314 L+ I Sbjct: 557 LQGLRNASVEEIAKVP 572 >lin1197 [L] COG0322 Nuclease subunit of the excinuclease complex Length = 603 Score = 342 bits (877), Expect = 5e-94, Method: Compositional matrix adjust. Identities = 166/266 (62%), Positives = 207/266 (77%), Gaps = 4/266 (1%) Query: 65 VKVLVPQRGLKKQMVGMACQNGRVALDEKFRLIERDEERTSGAAIXXXXXXXXXXXXRIE 124 K++VPQRG KK++V +A +N ++AL+EKF L+ER+EERT GA R+E Sbjct: 325 TKIIVPQRGNKKELVKLAYKNAKIALNEKFMLLERNEERTVGAVERLGEAMGIPTPSRVE 384 Query: 125 AFDNSNIQGTNPVSAMVVFIDGKPARKEYRKYKVRSVQGPDDYETMREVIRRRYERVLKE 184 AFDNSNI GT+PVSAMV F+DGKP++ +YRKYK+++V+GPDDY TMREVIRRRY RVLKE Sbjct: 385 AFDNSNIHGTDPVSAMVTFLDGKPSKNDYRKYKIKTVEGPDDYATMREVIRRRYWRVLKE 444 Query: 185 NLPQPDLIVVDGGKGQISSAIDILQNELGLYIPVCGLVKDDKHRTAQLLVGDSAEPVQLA 244 LP PDLI++DGGKGQI SA D+L NELGL IPV GL KDDKHRT+QLL GD E V L Sbjct: 445 GLPMPDLILIDGGKGQIDSAKDVLTNELGLDIPVAGLAKDDKHRTSQLLFGDPLEIVPLE 504 Query: 245 RDSQEFYLLQRIQDEVHRFAITFHREQRGKSMVTSKLDSIPGIGDKRRKLLLKHFGSLKK 304 R+SQEFYLLQR+QDEVHRFAITFHR+ R K+ S LD IPG+G R+K LLKHFGS+KK Sbjct: 505 RNSQEFYLLQRMQDEVHRFAITFHRQLRSKTGFQSILDGIPGVGPGRKKKLLKHFGSMKK 564 Query: 305 IKEASIEDFR----PLSIGDKLAGQI 326 +KEAS+E+ + PL++ +++ I Sbjct: 565 LKEASVEEIKEAGVPLNVAEEVHKHI 590 >ML0258 [FP] COG0248 Exopolyphosphatase Length = 346 Score = 271 bits (692), Expect = 2e-72, Method: Compositional matrix adjust. Identities = 0/286 (0%), Positives = 0/286 (0%), Gaps = 14/286 (4%) Query: 65 VKVLVPQRGLKKQMVGMACQNGRVALDEKFRLIERDEERTSGAAIXXXXXXXXXXXXRIE 124 Sbjct: 380 439 Query: 125 AFDNSNIQGTNPVSAMVVFIDGKPARKEYRKYKVRSVQGPDDYETMREVIRRRYERVLKE 184 Sbjct: 440 499 Query: 185 NLPQPDLIVVDGGKGQISSAIDILQNELGLYIPVCGLVKDDKHRTAQLLVGDSAEPVQLA 244 Sbjct: 500 - 558 Query: 245 RDSQEFYLLQRIQDEVHRFAITFHREQRGKSMVTSKLDSIPGIGDKRRKLLLKHFGSLKK 304 Sbjct: 559 618 Query: 305 IKEASIEDFR----PLSIGDK----LAGQI-----LDALKDEGPSL 337 Sbjct: 619 664 >CAC0508 [L] COG0322 Nuclease subunit of the excinuclease complex Length = 623 Score = 206 bits (525), Expect = 3e-53, Method: Compositional matrix adjust. Identities = 117/297 (39%), Positives = 184/297 (61%), Gaps = 28/297 (9%) Query: 59 LQEWLGVK------VLVPQRGLKKQMVGMACQNGRVALDEKFRLIERDEERTSGAAIXXX 112 L+ WL +K + +PQ+G KK ++ M +N ++ L EKF++ ++R + + Sbjct: 326 LENWLTMKRGSKVEIKIPQKGEKKDIIEMVKRNSKITL-EKFKIKLLSDKRLNENILIEM 384 Query: 113 XXXX--XXXXXRIEAFDNSNIQGTNPVSAMVVFIDGKPARKEYRKYKVRSVQGPDDYETM 170 RIEA+D SNIQG + V +M+VF +GKP +YR++K+++V+G +DY++M Sbjct: 385 TEVIGLEEVPHRIEAYDISNIQGVDSVGSMIVFEEGKPKNSDYRRFKIKTVKGANDYDSM 444 Query: 171 REVIRRRYERVLKE-------NLPQ--------PDLIVVDGGKGQISSAIDILQNELGLY 215 RE++ RR++ L+E NL PDLI++DGGKGQ++ A+++L+ E + Sbjct: 445 REILTRRFKHGLEEVNSIVNKNLSLSAGKFCVFPDLILMDGGKGQVNIALEVLK-EFNID 503 Query: 216 IPVCGLVKDDKHRTAQLLVGDSAEPVQLARDSQEFYLLQRIQDEVHRFAITFHREQRGKS 275 IPVCG+VKDD+H T ++ ++ ++ R F + R+QDEVHRFAIT+HR R K Sbjct: 504 IPVCGMVKDDRHNTRGIIYNNNEIDIKSNRKIINF--VTRVQDEVHRFAITYHRSLRDKR 561 Query: 276 MVTSKLDSIPGIGDKRRKLLLKHFGSLKKIKEASIEDF-RPLSIGDKLAGQILDALK 331 ++ S LD IP IG+KRRK LLKHFGS++ IK+A+ E+ + SI K A I+ + Sbjct: 562 VLHSVLDDIPYIGEKRRKALLKHFGSIENIKKATYEELMKTPSIDKKAAESIVSYFR 618 >Rv1420 [L] COG0322 Nuclease subunit of the excinuclease complex Length = 646 Score = 164 bits (415), Expect = 2e-40, Method: Compositional matrix adjust. Identities = 106/300 (35%), Positives = 164/300 (54%), Gaps = 32/300 (10%) Query: 59 LQEWL----GVKVL--VPQRGLKKQMVGMACQNGRVALDE-KFRLIERDEERTSGAAIXX 111 L WL G +V+ VP+RG K+ + +N AL + K + R++ Sbjct: 344 LASWLSGLRGSRVVLRVPRRGDKRALAETVHRNAEDALQQHKLKRASDFNARSAALQSIQ 403 Query: 112 XXXXXXXXXXRIEAFDNSNIQGTNPVSAMVVFIDGKPARKEYRKYKVRSVQG---PDDYE 168 RIE D S++QGT+ V ++VVF DG P + +YR + +R G DD Sbjct: 404 DSLGLADAPLRIECVDVSHVQGTDVVGSLVVFEDGLPRKSDYRHFGIREAAGQGRSDDVA 463 Query: 169 TMREVIRRRYERVLKENL------PQ---------PDLIVVDGGKGQISSAIDILQNELG 213 + EV RRR+ R L++ P+ P+L VVDGG Q+++A ++ +ELG Sbjct: 464 CIAEVTRRRFLRHLRDQSDPDLLSPERKSRRFAYPPNLYVVDGGAPQVNAASAVI-DELG 522 Query: 214 LY-IPVCGLVKDDKHRTAQLLVGDSAEPVQLARDSQEFYLLQRIQDEVHRFAITFHREQR 272 + + V GL K R ++ V +P+ + R+S+ YLLQR++DE HRFAIT+HR +R Sbjct: 523 VTDVAVIGLAK----RLEEVWVPSEPDPIIMPRNSEGLYLLQRVRDEAHRFAITYHRSKR 578 Query: 273 GKSMVTSKLDSIPGIGDKRRKLLLKHFGSLKKIKEASIEDFRPL-SIGDKLAGQILDALK 331 M S LDS+PG+G+ RRK L+ HFGS+ ++KEA++++ + IG A + DAL+ Sbjct: 579 STRMTASALDSVPGLGEHRRKALVTHFGSIARLKEATVDEITAVPGIGVATATAVHDALR 638 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.138 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,831,607 Number of extensions: 784373 Number of successful extensions: 2956 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 2931 Number of HSP's successfully gapped: 13 Length of query: 337 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 230 Effective length of database: 26,037,693 Effective search space: 5988669390 Effective search space used: 5988669390 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)