BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0434.0009.p (315 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1197 [L] COG0322 Nuclease subunit of the excinuclease complex 429 e-120 BS_uvrC [L] COG0322 Nuclease subunit of the excinuclease complex 429 e-120 ML0258 [FP] COG0248 Exopolyphosphatase 355 6e-98 CAC0508 [L] COG0322 Nuclease subunit of the excinuclease complex 269 5e-72 lin0839 [L] COG0322 Nuclease subunit of the excinuclease complex 261 1e-69 >lin1197 [L] COG0322 Nuclease subunit of the excinuclease complex Length = 603 Score = 429 bits (1104), Expect = e-120, Method: Compositional matrix adjust. Identities = 193/295 (65%), Positives = 241/295 (81%) Query: 5 DNIRNKLALLPDLPGCYLMKNEEGTIIYVGKAKVLKNRVRSYFTGSHNGKTQRLVANITD 64 ++I+NKLALLPD PGCYLMK+ +GTIIYVGKAKVLKNRVRSYF+G+H+ KTQRLV I D Sbjct: 4 EHIQNKLALLPDQPGCYLMKDRQGTIIYVGKAKVLKNRVRSYFSGTHDSKTQRLVQEIVD 63 Query: 65 FEYIVTSSNMEALILECNLIKKHMPRYNVLLKDDKTFPYLKITNEAHPRLEVTRRVIKDK 124 FEYIVTSSN+EAL+LE NLIKKH PR+N+ LKDDKT+P++KITNE HPRL +TR+V KDK Sbjct: 64 FEYIVTSSNVEALLLEINLIKKHDPRFNIRLKDDKTYPFIKITNERHPRLIITRQVKKDK 123 Query: 125 AKYFGPYPNAYAAHQTKKLLDRMYPLRKCGVMPKEVCLYYHMGQCLAPCEKEVPKSDYEE 184 KYFGPYPN YAA++ K++LDR+YPLRKC +P +VCLYYH+GQCLAPC +V S Y+E Sbjct: 124 GKYFGPYPNVYAANEVKRILDRLYPLRKCSTLPNKVCLYYHLGQCLAPCVFDVEASKYKE 183 Query: 185 ITQNIAGFLGGGHEAVKKDLQQKMQEAAEELYFERAKELRDQIIHIDALMEKQKINTADT 244 + I FL GG++ VK DL +KMQEAAE + FE+A E RDQI I+ MEKQK+ D Sbjct: 184 MQDEIVAFLNGGYKTVKNDLMKKMQEAAENMEFEKAGEFRDQINAIETTMEKQKMTMNDF 243 Query: 245 KDRDVFGYAVDKGWMCVQILYMRQGKMIQRHSSVFPFYGEAYSDFISYVAQYYSE 299 DRDVFGYA+DKGWMCVQ+ ++RQGK+I+R S FPFY +A DF++++ Q+Y + Sbjct: 244 VDRDVFGYAIDKGWMCVQVFFIRQGKLIERDVSQFPFYNDADEDFLTFIGQFYQK 298 >BS_uvrC [L] COG0322 Nuclease subunit of the excinuclease complex Length = 588 Score = 429 bits (1104), Expect = e-120, Method: Compositional matrix adjust. Identities = 16/294 (5%), Positives = 57/294 (19%) Query: 7 IRNKLALLPDLPGCYLMKNEEGTIIYVGKAKVLKNRVRSYFTGSHNGKTQRLVANITDFE 66 + + + + + + K + L +++ + +T+ L+ + Sbjct: 5 GGTVIYVGKAKDLKKRLSSYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIK 64 Query: 67 YIVTSSNMEALILECNLIKKHMPRYNVLLKDDKTFPYLKITNEAHPRLEVTRRVIKDKAK 126 N+ + + L + + K R + Sbjct: 65 LYQPRYNVLLRDDKSYPFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQ 124 Query: 127 YFGPYPNAYAAHQTKKLLDRMYPLRKCGVMPKEVCLYYHMGQCLAPCEKEVPKSDYEEIT 186 P + + + + P L + S ++ Sbjct: 125 KIFPIRQCENSVYRNRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQV 184 Query: 187 QNIAGFLGGGHEAVKKDLQQKMQEAAEELYFERAKELRDQIIHIDALMEKQKINTADTKD 246 + + + ++ D + + Sbjct: 185 LTQLISRMETASQNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACV 244 Query: 247 RDVFGYAVDKGWMCVQILYMRQGKMIQRHSSVFPFYGEAYSDFISYVAQYYSEN 300 +F + G + F + + + Sbjct: 245 HVLFIRQGKVLGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLD 298 >ML0258 [FP] COG0248 Exopolyphosphatase Length = 346 Score = 355 bits (911), Expect = 6e-98, Method: Compositional matrix adjust. Identities = 24/295 (8%), Positives = 59/295 (20%) Query: 6 NIRNKLALLPDLPGCYLMKNEEGTIIYVGKAKVLKNRVRSYFTGSHNGKTQRLVANITDF 65 + +L G M E A++ + T Sbjct: 4 HRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQG 63 Query: 66 EYIVTSSNMEALILECNLIKKHMPRYNVLLKDDKTFPYLKITNEAHPRLEVTRRVIKDKA 125 N A + +DK + + + R + + + + Sbjct: 64 LTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQT 123 Query: 126 KYFGPYPNAYAAHQTKKLLDRMYPLRKCGVMPKEVCLYYHMGQCLAPCEKEVPKSDYEEI 185 Y Y + T Y + + + + E Sbjct: 124 DYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETA 183 Query: 186 TQNIAGFLGGGHEAVKKDLQQKMQEAAEELYFERAKELRDQIIHIDALMEKQKINTADTK 245 + + + + + + FE Q ++ L T Sbjct: 184 FGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG 243 Query: 246 DRDVFGYAVDKGWMCVQILYMRQGKMIQRHSSVFPFYGEAYSDFISYVAQYYSEN 300 G+ Q+ + + + + +A + Sbjct: 244 KYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRR 298 >CAC0508 [L] COG0322 Nuclease subunit of the excinuclease complex Length = 623 Score = 269 bits (687), Expect = 5e-72, Method: Compositional matrix adjust. Identities = 138/314 (43%), Positives = 203/314 (64%), Gaps = 11/314 (3%) Query: 10 KLALLPDLPGCYLMKNEEGTIIYVGKAKVLKNRVRSYFTGS--HNGKTQRLVANITDFEY 67 +L LPD PG Y+MKN G +IYVGKAK+LKNRVR YF S H+ K + +V NI++FEY Sbjct: 7 QLKNLPDKPGVYIMKNSLGEVIYVGKAKILKNRVRQYFRNSKNHSEKVKAMVKNISEFEY 66 Query: 68 IVTSSNMEALILECNLIKKHMPRYNVLLKDDKTFPYLKIT-NEAHPRLEVTRRVIKDKAK 126 IVT S +EALILECNLIKK+ PRYN+LLKDDK +P++K+T NE PR+ VTR ++KD +K Sbjct: 67 IVTDSEIEALILECNLIKKYKPRYNILLKDDKHYPFIKVTMNEDFPRIIVTRNMVKDGSK 126 Query: 127 YFGPYPNAYAAHQTKKLLDRMYPLRKCGVMPKE------VCLYYHMGQCLAPCEKEVPKS 180 YFGPYP+ A H+T L+ R++P+R C KE CL YH+ +C APC + K Sbjct: 127 YFGPYPDVSAVHETVDLMRRIFPIRTCKKYIKENGENIRPCLNYHIKRCNAPCAGLISKE 186 Query: 181 DYEEITQNIAGFLGGGHEAVKKDLQQKMQEAAEELYFERAKELRDQIIHIDALMEKQKIN 240 +Y EI + G + G + + ++L+++M++A+ L FE+A +LRD+++ + EKQKI Sbjct: 187 EYGEIIKKAVGLISGKNNDIIRELKEEMEKASMNLDFEKAADLRDKMLAAQKVTEKQKII 246 Query: 241 TADTKDRDVFGYAVDKGWMCVQILYMRQGKMIQR-HSSVFPFYGEAYSDFIS-YVAQYYS 298 + ++ D D+ CVQ+ ++R+GK++ R H V GE D I ++ ++YS Sbjct: 247 IGNFENEDYISLYSDEKDSCVQVFFLREGKIVGREHFIVENTAGENEGDIIGEFIKEFYS 306 Query: 299 ENPALXXXXNSAAG 312 + ++AG Sbjct: 307 GTAYVPKSIYASAG 320 >lin0839 [L] COG0322 Nuclease subunit of the excinuclease complex Length = 366 Score = 261 bits (666), Expect = 1e-69, Method: Compositional matrix adjust. Identities = 129/297 (43%), Positives = 187/297 (62%), Gaps = 1/297 (0%) Query: 5 DNIRNKLALLPDLPGCYLMKNEEGTIIYVGKAKVLKNRVRSYFTGSHNGKTQRLVANITD 64 D ++ KL LLP+ PGCY+ ++E I+Y+GK+K LKNRV+SYF GKT RLV I D Sbjct: 3 DKLQTKLTLLPESPGCYIYRDENNEILYIGKSKCLKNRVKSYFHSKQIGKTARLVRQIRD 62 Query: 65 FEYIVTSSNMEALILECNLIKKHMPRYNVLLKDDKTFPYLKITNEAHPRLEVTRRVIKDK 124 E I+T+S EAL+LE LI+K+ P +N+ LK+ ++PY+KITNE +P +EV V +D Sbjct: 63 LELIITTSEKEALLLEMILIQKYQPPFNIQLKEGTSYPYIKITNEKNPHIEVVFEVKQDG 122 Query: 125 AKYFGPYPNAYAAHQTKKLLDRMYPLRKCGVMPKEVCLYYHMGQCLAPCEKEVPKSDYEE 184 A YFGPYP ++A QT +L++++YPL +C P CLYYH+G CL PC+ E+ + Y++ Sbjct: 123 AHYFGPYPGRFSARQTVELIEKLYPLCRCDGKPGRPCLYYHLGLCLGPCQAEIEPAVYKK 182 Query: 185 ITQNIAGFLGGGHEAVKKDLQQKMQEAAEELYFERAKELRDQIIHIDALMEKQKINTADT 244 Q I+ FL G + VK L + M+ A E+L FERA ELRD+I I+ +EKQ I Sbjct: 183 QIQKISRFLEGDVKGVKGKLTKDMEAATEKLEFERAAELRDKIHAINETIEKQHIIFPGL 242 Query: 245 KDRDVFGYAVDKGWMCVQILYMRQGKMIQRHSSVFPFYGEAYSDFISYVAQYYSENP 301 K+RD+ G + V + ++R G + VFP D +++ Q+Y E+P Sbjct: 243 KNRDIIGCYEQDNHLSVFVFFVRNGAINGSKWHVFPIENSLQEDLANFINQFY-EDP 298 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.137 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 22,340,046 Number of extensions: 869878 Number of successful extensions: 1412 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 1367 Number of HSP's successfully gapped: 21 Length of query: 315 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 209 Effective length of database: 26,153,027 Effective search space: 5465982643 Effective search space used: 5465982643 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)