BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0430.0008.p (250 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSp0085 [M] COG3209 Rhs family protein 65 1e-10 ECs2061 [M] COG3209 Rhs family protein 61 2e-09 Z0651 [M] COG3209 Rhs family protein 57 2e-08 ECs0560 [M] COG3209 Rhs family protein 57 2e-08 rhsD [M] COG3209 Rhs family protein 57 2e-08 >RSp0085 [M] COG3209 Rhs family protein Length = 818 Score = 64.7 bits (156), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 69/229 (30%), Positives = 96/229 (41%), Gaps = 44/229 (19%) Query: 3 LQFGLSYLSRDPY-KGVNG----LGWHHSYEWKLNPLNGGKVEVMSPDGSLFEYVPLAGG 57 L L+Y S Y KGV G LGW S + L + +V V DGS+ + Sbjct: 117 LPITLAYRSSSRYGKGVGGGQWMLGWQRSLDTSLAAESTPQVAVQRDDGSVSTF------ 170 Query: 58 QYLTPRGTDWTLSAPSSGEYTLASPQGDLYRFNGEGLLQSVTDLNGNQIAFAYQGKKLEE 117 TP GT W+ S N + LQ +TD NG + Y Sbjct: 171 ---TPNGTAWSAS-------------------NTQDTLQPITDANGKTTGWQY-----TV 203 Query: 118 VSTAGASLSLSYDGAGKLIAVSDLTGRQMSFHYDPASGDMTSITDVDGATTKLTYDDNHH 177 V T A +YD +GKL +V D GR + Y+ A+ +T++T G + + YD + Sbjct: 204 VDTGAAE---TYDTSGKLQSVRDRNGRTTTLAYN-AANQLTTVTAPSGRSLAVAYDSQNR 259 Query: 178 VLSVTDAKGTAVMSVAYDDHQRVTSFTDFYGNTSSSAYE-GRVAPVVRG 225 V SVT G AV Y+ +++ T G T YE GR V+ G Sbjct: 260 VASVTAPSG-AVTGYGYNGAGILSTVTHPDGTTRQYVYEDGRFPAVLTG 307 >ECs2061 [M] COG3209 Rhs family protein Length = 1400 Score = 61.2 bits (147), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 48/141 (34%), Positives = 70/141 (49%), Gaps = 6/141 (4%) Query: 81 SPQGDLYRFNGEGLLQSVTD-LNGNQIAFAYQGKKLEEV---STAGASLSLS-YDGAGKL 135 +P G YR+ E +VTD LN ++ G L+ V A S++ S YD AG+L Sbjct: 379 NPAGLSYRYQYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRL 438 Query: 136 IAVSDLTGRQMSFHYDPASGDMTSITDVDGATTKLTYDDNHHVLSVTDAKGTAVMSVAYD 195 A +D GR+ + + SGD+T IT DG TK Y+D + + +V G AYD Sbjct: 439 TAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRR-AYD 497 Query: 196 DHQRVTSFTDFYGNTSSSAYE 216 + R+ S T G+ AY+ Sbjct: 498 EPGRLVSETSRCGDVIRYAYD 518 Score = 53.1 bits (126), Expect = 4e-07, Method: Compositional matrix adjust. Identities = 38/126 (30%), Positives = 61/126 (48%), Gaps = 5/126 (3%) Query: 93 GLLQSVTDLNGNQIAFAYQ--GKKLEEVSTAGASLSLSYDGAGKLIAVSDLTGRQMSFHY 150 G L + TD +G Q + Y G+ G S YD G+L +V D G + + Y Sbjct: 544 GQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISRYRRYDNRGRLTSVKDAQGHETRYEY 603 Query: 151 DPASGDMTSITDVDGATTKLTYDDNHHVLSVTDAKGTAVMSVAYDDHQRVTSFTDFYGNT 210 + A+GD+T++ DG ++ YD +S T +G S+ YD R+T+ T+ G+ Sbjct: 604 N-AAGDLTAVITPDGNRSETQYDAWGKAVSTT--QGGLTRSMEYDLAGRITTLTNENGSR 660 Query: 211 SSSAYE 216 S Y+ Sbjct: 661 SEFTYD 666 >Z0651 [M] COG3209 Rhs family protein Length = 1398 Score = 57.4 bits (137), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 47/141 (33%), Positives = 68/141 (48%), Gaps = 6/141 (4%) Query: 81 SPQGDLYRFNGEGLLQSVTD-LNGNQIAFAYQGKKLEEV---STAGASLSLS-YDGAGKL 135 +P G Y + E +VTD LN ++ G L+ V A S++ S YD AG+L Sbjct: 379 NPAGLSYHYQYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRL 438 Query: 136 IAVSDLTGRQMSFHYDPASGDMTSITDVDGATTKLTYDDNHHVLSVTDAKGTAVMSVAYD 195 A +D GR+ + + SGD+T IT DG TK Y+D + + +V G AYD Sbjct: 439 TAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRR-AYD 497 Query: 196 DHQRVTSFTDFYGNTSSSAYE 216 + R+ S T G T Y+ Sbjct: 498 EPGRLVSETSRSGETVRYRYD 518 Score = 57.4 bits (137), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 40/127 (31%), Positives = 63/127 (49%), Gaps = 5/127 (3%) Query: 93 GLLQSVTDLNGNQIAFAYQ--GKKLEEVSTAGASLSLSYDGAGKLIAVSDLTGRQMSFHY 150 G L + TD +G Q + Y G+ G SL YD G+LI+V D G + + Y Sbjct: 544 GQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLISVKDAQGHETRYEY 603 Query: 151 DPASGDMTSITDVDGATTKLTYDDNHHVLSVTDAKGTAVMSVAYDDHQRVTSFTDFYGNT 210 + A+GD+T++ DG ++ YD +S T +G S+ YD R+T+ T+ G+ Sbjct: 604 N-AAGDLTAVITPDGNRSETQYDAWGKAVSTT--QGGLTRSMEYDLAGRITTLTNENGSR 660 Query: 211 SSSAYEG 217 S Y+ Sbjct: 661 SEFTYDA 667 >ECs0560 [M] COG3209 Rhs family protein Length = 1398 Score = 57.4 bits (137), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 47/141 (33%), Positives = 68/141 (48%), Gaps = 6/141 (4%) Query: 81 SPQGDLYRFNGEGLLQSVTD-LNGNQIAFAYQGKKLEEV---STAGASLSLS-YDGAGKL 135 +P G Y + E +VTD LN ++ G L+ V A S++ S YD AG+L Sbjct: 379 NPAGLSYHYQYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRL 438 Query: 136 IAVSDLTGRQMSFHYDPASGDMTSITDVDGATTKLTYDDNHHVLSVTDAKGTAVMSVAYD 195 A +D GR+ + + SGD+T IT DG TK Y+D + + +V G AYD Sbjct: 439 TAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRR-AYD 497 Query: 196 DHQRVTSFTDFYGNTSSSAYE 216 + R+ S T G T Y+ Sbjct: 498 EPGRLVSETSRSGETVRYRYD 518 Score = 57.4 bits (137), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 40/127 (31%), Positives = 63/127 (49%), Gaps = 5/127 (3%) Query: 93 GLLQSVTDLNGNQIAFAYQ--GKKLEEVSTAGASLSLSYDGAGKLIAVSDLTGRQMSFHY 150 G L + TD +G Q + Y G+ G SL YD G+LI+V D G + + Y Sbjct: 544 GQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLISVKDAQGHETRYEY 603 Query: 151 DPASGDMTSITDVDGATTKLTYDDNHHVLSVTDAKGTAVMSVAYDDHQRVTSFTDFYGNT 210 + A+GD+T++ DG ++ YD +S T +G S+ YD R+T+ T+ G+ Sbjct: 604 N-AAGDLTAVITPDGNRSETQYDAWGKAVSTT--QGGLTRSMEYDLAGRITTLTNENGSR 660 Query: 211 SSSAYEG 217 S Y+ Sbjct: 661 SEFTYDA 667 >rhsD [M] COG3209 Rhs family protein Length = 1426 Score = 57.4 bits (137), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 41/127 (32%), Positives = 63/127 (49%), Gaps = 5/127 (3%) Query: 93 GLLQSVTDLNGNQIAFAYQ--GKKLEEVSTAGASLSLSYDGAGKLIAVSDLTGRQMSFHY 150 G L + TD +G Q + Y G+ G SL YD G+L +V D GR+ + Y Sbjct: 541 GQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGISLYRRYDNRGRLTSVKDAQGRETRYEY 600 Query: 151 DPASGDMTSITDVDGATTKLTYDDNHHVLSVTDAKGTAVMSVAYDDHQRVTSFTDFYGNT 210 + A+GD+T++ DG ++ YD +S T +G S+ YD RV S T+ G+ Sbjct: 601 N-AAGDLTAVITPDGNRSETQYDAWGKAVSTT--QGGLTRSMEYDAAGRVISLTNENGSH 657 Query: 211 SSSAYEG 217 S +Y+ Sbjct: 658 SVFSYDA 664 Score = 57.0 bits (136), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 47/141 (33%), Positives = 68/141 (48%), Gaps = 6/141 (4%) Query: 81 SPQGDLYRFNGEGLLQSVTD-LNGNQIAFAYQGKKLEEV---STAGASLSLS-YDGAGKL 135 +P G YR+ E +VTD LN ++ G L+ V A S++ S YD AG+L Sbjct: 376 NPAGLSYRYLYEQDRITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTRSGYDAAGRL 435 Query: 136 IAVSDLTGRQMSFHYDPASGDMTSITDVDGATTKLTYDDNHHVLSVTDAKGTAVMSVAYD 195 A +D GR+ + + SGD+T IT DG TK Y+D + + +V G YD Sbjct: 436 TAQTDAAGRRTEYGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRR-EYD 494 Query: 196 DHQRVTSFTDFYGNTSSSAYE 216 + R+ S T G T Y+ Sbjct: 495 EPGRLVSETSRSGETVRYRYD 515 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,783,362 Number of extensions: 900139 Number of successful extensions: 2307 Number of sequences better than 1.0e-05: 15 Number of HSP's gapped: 2240 Number of HSP's successfully gapped: 27 Length of query: 250 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 146 Effective length of database: 26,383,695 Effective search space: 3852019470 Effective search space used: 3852019470 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 114 (48.5 bits)