BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0430.0002.p (397 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH1535 [M] COG1686 D-alanyl-D-alanine carboxypeptidase 398 e-111 BS_dacF [M] COG1686 D-alanyl-D-alanine carboxypeptidase 393 e-109 CAC2063 [M] COG1686 D-alanyl-D-alanine carboxypeptidase 311 1e-84 PA3999 [M] COG1686 D-alanyl-D-alanine carboxypeptidase 222 1e-57 VC0947 [M] COG1686 D-alanyl-D-alanine carboxypeptidase 182 1e-45 >BH1535 [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 387 Score = 398 bits (1023), Expect = e-111, Method: Compositional matrix adjust. Identities = 189/352 (53%), Positives = 261/352 (74%) Query: 40 NLAPGARSAILMDANTGTVVYEKNSHDKLPPASITKIMTMLLTVEALDEGKLQLTDKVRT 99 NLA A SAI+++ +TG V++EKNS +KLPPAS+TKIMT+LL +EA+D GK+ D VRT Sbjct: 30 NLAKEASSAIVIERDTGQVLFEKNSDEKLPPASMTKIMTLLLIMEAIDSGKITYDDMVRT 89 Query: 100 SEYAASMGGSQIFLEPGEEMTVDEMLKGIAMASGNDASVAMAEKLAGSESAFVDLMNQRA 159 SE AASMGGSQ+FLEPGEEM+V +M+K IA+ASGNDASVAMAE LAG+E FV +MN++A Sbjct: 90 SENAASMGGSQVFLEPGEEMSVRDMIKAIAIASGNDASVAMAEHLAGTEEEFVGMMNEKA 149 Query: 160 GELGLKDTHFANCNGLPAENHYSSAHDIAVISKELLKHERIIKYTGSYQDYLRKDTTKPF 219 +LGLK+T+F N NGLP ++HY+SA+D+A+ISKELLK+E I ++T Y+DYLRK T K F Sbjct: 150 RQLGLKNTNFVNTNGLPEKDHYTSAYDLAMISKELLKYEDITEFTSVYEDYLRKGTDKEF 209 Query: 220 WLVNTNKLVRFYTGADGLKTGYTAEAKFCLSATAARDGLRAIAVVLGEPNTKTRNSEVSG 279 WLVNTN+LV+FY G DGLKTG+T EAK+CL+ATA ++G+R I VV+G P K RNS+++ Sbjct: 210 WLVNTNRLVKFYPGVDGLKTGFTKEAKYCLTATAVKNGMRVITVVMGAPTPKERNSQITE 269 Query: 280 MFDYLFSQYKVHTIHKAGEAIGNVKIEKGVKTKLPLTAKETYSVLLKKGITQEGIRHELV 339 M D+ FSQY+ H +++ + ++ + KG K +P+ E+ S++ KKG + + + + Sbjct: 270 MLDFAFSQYQTHQVYERDTYMMDLNVRKGNKKSVPIFTSESVSLVTKKGESIDNVTERVE 329 Query: 340 VPESIKAPVKAGQAVGKLVVYQGTDKIKEYELKADTDVPKAGWWKLFKRTAG 391 ES+ APV G VG L + + + + E L A DV A +W++FKR G Sbjct: 330 WKESLLAPVHKGDVVGTLYLERDGEILSETPLIAGEDVASASFWQMFKRIVG 381 >BS_dacF [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 389 Score = 393 bits (1010), Expect = e-109, Method: Compositional matrix adjust. Identities = 199/365 (54%), Positives = 273/365 (74%), Gaps = 2/365 (0%) Query: 27 AKAKDTGKNAAVVNLAPGARSAILMDANTGTVVYEKNSHDKLPPASITKIMTMLLTVEAL 86 A AK GK + LA A+SA+L++ +TG V+Y KNS+++L PAS+TKIMTMLL +EAL Sbjct: 21 AFAKQDGKRTS--ELAHEAKSAVLIERDTGKVLYNKNSNERLAPASMTKIMTMLLIMEAL 78 Query: 87 DEGKLQLTDKVRTSEYAASMGGSQIFLEPGEEMTVDEMLKGIAMASGNDASVAMAEKLAG 146 D+GK++++DKVRTSE+AASMGGSQIFLEPGEEMTV EMLKGIA+ASGNDASVAMAE ++G Sbjct: 79 DKGKIKMSDKVRTSEHAASMGGSQIFLEPGEEMTVKEMLKGIAIASGNDASVAMAEFISG 138 Query: 147 SESAFVDLMNQRAGELGLKDTHFANCNGLPAENHYSSAHDIAVISKELLKHERIIKYTGS 206 SE FV MN++A ELGLK+T F N GL E HYSSA+D+A+++KELLK+E I K+TG+ Sbjct: 139 SEEEFVKKMNKKAKELGLKNTSFKNPTGLTEEGHYSSAYDMAIMAKELLKYESITKFTGT 198 Query: 207 YQDYLRKDTTKPFWLVNTNKLVRFYTGADGLKTGYTAEAKFCLSATAARDGLRAIAVVLG 266 Y+DYLR++T K FWLVNTN+L++FY G DG+KTGYT EAK+CL+A+A + +RAIAVV G Sbjct: 199 YEDYLRENTDKKFWLVNTNRLIKFYPGVDGVKTGYTGEAKYCLTASAKKGNMRAIAVVFG 258 Query: 267 EPNTKTRNSEVSGMFDYLFSQYKVHTIHKAGEAIGNVKIEKGVKTKLPLTAKETYSVLLK 326 K RN++V+ M D+ FSQY+ H ++K + + VK++KG + + LT E S+L K Sbjct: 259 ASTPKERNAQVTKMLDFAFSQYETHPLYKRNQTVAKVKVKKGKQKFIELTTSEPISILTK 318 Query: 327 KGITQEGIRHELVVPESIKAPVKAGQAVGKLVVYQGTDKIKEYELKADTDVPKAGWWKLF 386 KG ++ E+ + ++I AP++ GQ +G LV+ + + + E + A D+ KAG+ Sbjct: 319 KGEDMNDVKKEIKMKDNISAPIQKGQELGTLVLKKDGEVLAESPVAAKEDMKKAGFITFL 378 Query: 387 KRTAG 391 KRT G Sbjct: 379 KRTMG 383 >CAC2063 [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 404 Score = 311 bits (798), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 156/350 (44%), Positives = 229/350 (65%) Query: 45 ARSAILMDANTGTVVYEKNSHDKLPPASITKIMTMLLTVEALDEGKLQLTDKVRTSEYAA 104 A+SAIL++ +TG ++YEKNSH+K PAS+TKIMTMLLT+EA+D GK++LTDKV SE A Sbjct: 51 AKSAILLEPSTGAIIYEKNSHEKFAPASVTKIMTMLLTMEAVDSGKIKLTDKVTVSENAK 110 Query: 105 SMGGSQIFLEPGEEMTVDEMLKGIAMASGNDASVAMAEKLAGSESAFVDLMNQRAGELGL 164 MGGS + L+ GE TV+E+LKGIA+ASGNDA+VAMAE L GS+ FV++MN++A E+G+ Sbjct: 111 KMGGSSMLLDTGEVRTVEELLKGIAIASGNDAAVAMAEYLGGSQENFVNMMNKKAQEIGM 170 Query: 165 KDTHFANCNGLPAENHYSSAHDIAVISKELLKHERIIKYTGSYQDYLRKDTTKPFWLVNT 224 KDT F NC GL A+ H ++A+DI+++S+ELLKH +I+K+TG+Y + + + P LVN Sbjct: 171 KDTSFKNCTGLNADGHMTTAYDISLMSRELLKHPKILKFTGTYMETISEGRKTPIALVNH 230 Query: 225 NKLVRFYTGADGLKTGYTAEAKFCLSATAARDGLRAIAVVLGEPNTKTRNSEVSGMFDYL 284 NKLVRF+ G DGLKTG+T EAK+C+SATA +D +R +A+++G P K RN + S + +Y Sbjct: 231 NKLVRFFKGCDGLKTGFTDEAKYCISATACKDNVRMLAIIMGAPTYKVRNRDASMLMNYG 290 Query: 285 FSQYKVHTIHKAGEAIGNVKIEKGVKTKLPLTAKETYSVLLKKGITQEGIRHELVVPESI 344 FS++ + + + V + K + A + SV++ KG L + Sbjct: 291 FSKFTAKKVVEKDSVVKKVALNKKGDKYFLIKAVDDLSVVIPKGENCNITNRCLYKIDKK 350 Query: 345 KAPVKAGQAVGKLVVYQGTDKIKEYELKADTDVPKAGWWKLFKRTAGSMF 394 +K G +G Y + ++ D DV G+++ FK +F Sbjct: 351 DKEIKKGAYIGYAEYYVNGKYAGKIKVYCDRDVKLGGFFQNFKSDLQRIF 400 >PA3999 [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 386 Score = 222 bits (565), Expect = 1e-57, Method: Compositional matrix adjust. Identities = 122/340 (35%), Positives = 201/340 (59%), Gaps = 5/340 (1%) Query: 45 ARSAILMDANTGTVVYEKNSHDKLPPASITKIMTMLLTVEALDEGKLQLTDKVRTSEYAA 104 A+S +LMD +G V+ E N +LPPAS+TK+MT + + ++ G++ D V SE+A Sbjct: 37 AKSYVLMDGESGQVLVENNGDQRLPPASLTKLMTAYIATKEIEAGRIGENDLVTVSEHAW 96 Query: 105 SMGGSQIFLEPGEEMTVDEMLKGIAMASGNDASVAMAEKLAGSESAFVDLMNQRAGELGL 164 GGS++F++ G +++V ++L GI + SGNDASVA+AE +AGSE AF D+MN A +LGL Sbjct: 97 RTGGSRMFIKVGSQVSVSDLLHGIIIQSGNDASVALAEHIAGSEDAFADMMNTTAQKLGL 156 Query: 165 KDTHFANCNGLPAENHYSSAHDIAVISKELLKHERIIKYTGSYQDYLRKDTTKPFWLVNT 224 ++HF + GLP +HYSSA D+AV+++ ++ E + +++L + +P N Sbjct: 157 TNSHFMDATGLPNPDHYSSARDMAVLARAIIYGEPSHYAIYAQKEFLWNNIKQP----NR 212 Query: 225 NKLVRFYTGADGLKTGYTAEAKFCLSATAARDGLRAIAVVLGEPNTKTRNSEVSGMFDYL 284 N L+ DGLKTG+T EA +CL A+A RDG R IAVV G + + R +E + Y Sbjct: 213 NLLLWRDKTVDGLKTGHTDEAGYCLVASAVRDGQRMIAVVFGTNSEQARAAETQKLLTYG 272 Query: 285 FSQYKVHTIHKAGEAIGNVKIEKGVKTKLPLTAKETYSVLLKKGITQEGIRHELVVPESI 344 F ++ +K G + + KG + ++ E ++ L +G Q+ ++ +V+ + Sbjct: 273 FRFFESRNFYKKGTELTKGLVWKGSEHEVKAGLAEDLTMTLPRGQMQK-LQASMVLEPQL 331 Query: 345 KAPVKAGQAVGKLVVYQGTDKIKEYELKADTDVPKAGWWK 384 AP++ GQ +GK+ V I+ +L A V + G+++ Sbjct: 332 MAPIQQGQVIGKVEVKLDDKVIRSADLVALNAVEEGGFFR 371 >VC0947 [M] COG1686 D-alanyl-D-alanine carboxypeptidase Length = 391 Score = 182 bits (461), Expect = 1e-45, Method: Compositional matrix adjust. Identities = 126/357 (35%), Positives = 189/357 (52%), Gaps = 30/357 (8%) Query: 45 ARSAILMDANTGTVVYEKNSHDKLPPASITKIMTMLLTVEALDEGKLQLTDKVRTSEYAA 104 A+ +LMD ++G V+ EK KL PAS+TK+MT + + + G + L D V S+ A Sbjct: 39 AKGYVLMDYHSGKVLAEKEMDTKLSPASLTKMMTSYVIGQEVKRGNISLNDDVVISKNAW 98 Query: 105 SMG---GSQIFLEPGEEMTVDEMLKGIAMASGNDASVAMAEKLAGSESAFVDLMNQRAGE 161 + S++F+E G + V ++ +GI + SGNDA VAMAE +AG+E AFVDLMN A Sbjct: 99 AKNFPDSSKMFVEVGTTVKVSDLNRGIIIQSGNDACVAMAEHVAGTEDAFVDLMNAWASS 158 Query: 162 LGLKDTHFANCNGLPAENHYSSAHDIAVISKELLK---------HERIIKYTGSYQDYLR 212 LG+K++HF N +GL N YS+ +D+A++ + L++ E+ Y G Q Y R Sbjct: 159 LGMKNSHFTNSHGLDDPNLYSTPYDLALLGQALIRXVPEEYAIYSEQKFTYNGITQ-YNR 217 Query: 213 KDTTKPFWLVNTNKLVRFYTGADGLKTGYTAEAKFCLSATAARDGLRAIAVVLGEPNTKT 272 W + N DG+KTG+T+ A + L ++A +R +AVV+G N Sbjct: 218 NGL---LWDKSMN--------VDGIKTGHTSGAGYNLVSSATEGNMRLVAVVMGTDNENA 266 Query: 273 RNSEVSGMFDYLFSQYKVHTIHKAGEAIGNVKIEKGVKTKLPL-TAKETYSVLLKKGITQ 331 R +E + Y F ++ HKAGE N I G K + L K+TY V L +G + Sbjct: 267 RKAESKKLLSYGFRFFETVAPHKAGETFVNETIWMGDKDTIALGVDKDTY-VTLPRGQAK 325 Query: 332 EGIRHELVVPESIKAPVKAGQAVGKLVVYQGTDKIKEYELKADTDVPKAGWWKLFKR 388 + + V+ + +KAP+K G VG L + I +Y L A DV + LF R Sbjct: 326 D-LTASFVLEKQLKAPLKKGDIVGTLYYQLAGNDIAQYPLLALEDVQEG---SLFSR 378 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.314 0.130 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,729,847 Number of extensions: 1057160 Number of successful extensions: 3080 Number of sequences better than 1.0e-05: 72 Number of HSP's gapped: 2940 Number of HSP's successfully gapped: 72 Length of query: 397 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 288 Effective length of database: 25,807,025 Effective search space: 7432423200 Effective search space used: 7432423200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 117 (49.7 bits)