BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0427.0010.p (269 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value TM1368 [O] COG0396 ABC-type transport system involved in Fe-S cl... 176 5e-44 STM1368 [R] COG1823 Predicted Na+/dicarboxylate symporter 176 5e-44 PH1384 [O] COG0396 ABC-type transport system involved in Fe-S cl... 113 3e-25 TM1417 [O] COG0396 ABC-type transport system involved in Fe-S cl... 97 2e-20 MA4406 [O] COG0396 ABC-type transport system involved in Fe-S cl... 97 3e-20 >TM1368 [O] COG0396 ABC-type transport system involved in Fe-S cluster assembly, ATPase component Length = 246 Score = 176 bits (445), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 96/212 (45%), Positives = 132/212 (62%), Gaps = 6/212 (2%) Query: 41 IMGPNGTGKSTLASALMGHPKYEVTNGRVTXXXXXXXXXXXXXRARAGLFLAMQYPSEIA 100 +MGPNG+GKSTLA+ +MG+P+Y VT G + RA+ G+ + Q P E+ Sbjct: 34 LMGPNGSGKSTLANVIMGNPRYIVTEGDIVFEGNSIKDLPPNERAKLGIMMTFQNPYEVE 93 Query: 101 GVTNSDFLRSAVNARRGEGNEVSLIRFIRQMESKMKGLEMNPEFSHRXXXXXXEGFSGGE 160 GV S FL +A RR G + + + +++E + L ++ +F R GFSGGE Sbjct: 94 GVKLSQFLITA--HRRIHGEDKNYLELRKELEETAEKLGLDKDFLERYLNV---GFSGGE 148 Query: 161 KKRNEILQMMMLEPKFVILDEVDSGLDIDALRIVANGVNELRSPDRGFLIITHYQRLLNY 220 KKR+EILQ + L PK +ILDE+DSGLD+DALR++AN + L LIITHY+RLL++ Sbjct: 149 KKRSEILQSLFLRPKLLILDEIDSGLDVDALRLIANLIARLNEEGVTLLIITHYKRLLDH 208 Query: 221 ITP-DFVHVMMQGRIVKSGGPELAHRLEADGY 251 + D VHV + GRIV SGGPELA +E GY Sbjct: 209 LKRIDKVHVYVDGRIVTSGGPELADEIEEKGY 240 >STM1368 [R] COG1823 Predicted Na+/dicarboxylate symporter Length = 448 Score = 176 bits (445), Expect = 5e-44, Method: Compositional matrix adjust. Identities = 10/212 (4%), Positives = 32/212 (15%), Gaps = 6/212 (2%) Query: 41 IMGPNGTGKSTLASALMGHPKYEVTNGRVTXXXXXXXXXXXXXRARAGLFLAMQYPSEIA 100 I G + + + + + Sbjct: 34 IAIFLGAVIGLSLHFISANHTKTIIEWYSIVGNGYVHLLKLVAIPLIFISILSAINKLEN 93 Query: 101 GVTNSDFLRSAVNARRGEGNEVSLIRFIRQMESKMKGLEMNPEFSHRXXXXXXEGFSGGE 160 + + + Sbjct: 94 SAGIGKMSLTI--VGCMLCLVMVAGFIGLLTAHVLGLDASAFVHMPSMLTT---EEVNKT 148 Query: 161 KKRNEILQMMMLEPKFVILDEVDSGLDIDALRIVANGVNELRSPDRGFLIITHYQRLLNY 220 + + L P + LD + ++ + + Q+L Sbjct: 149 AAVSIPQLVTSLIPTNIFLDLTGARSVSVIGIVIFTLIAGIALLKVKKEAPEEGQKLSAG 208 Query: 221 ITP-DFVHVMMQGRIVKSGGPELAHRLEADGY 251 I + M ++ + + Sbjct: 209 INAIQIWVMKMVRIVIALTPYGVMALMTTVFS 240 >PH1384 [O] COG0396 ABC-type transport system involved in Fe-S cluster assembly, ATPase component Length = 247 Score = 113 bits (283), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 84/219 (38%), Positives = 117/219 (53%), Gaps = 15/219 (6%) Query: 41 IMGPNGTGKSTLASALMGHPKYEVTNGRVTXXXXXXXXXXXXXRARAGLFLAMQYPSEIA 100 IMGPNG+GKSTLA L GHP Y+VT G + RA+ G+ LA Q P EI Sbjct: 32 IMGPNGSGKSTLALTLAGHPNYKVTKGGIFFEDKEITNLTPDERAKMGILLAFQVPPEIQ 91 Query: 101 GVTNSDFLRSAVNARRGEGNEVSLIRFIRQMESKMKGLEMNPEFSHRXXXXXXE---GFS 157 GV DFL + S I+ + E+ +E E R GFS Sbjct: 92 GVRIIDFLTQTL----------SEIKNLDASEAYNIIIEKAKELWFREDDLRRYINVGFS 141 Query: 158 GGEKKRNEILQMMMLEPKFVILDEVDSGLDIDALRIVANGVNELRSPDRGFLIITHYQRL 217 GGE+KR E+LQ ++LEPK +ILDE DSG+D+D+L +++ + EL ++ITHY R+ Sbjct: 142 GGERKRLELLQALLLEPKLLILDEPDSGVDVDSLSVISRKIEELHKKGVSIILITHYGRI 201 Query: 218 LNYITPD--FVHVMMQGRIVKSGGPELAHRLEADGYDWV 254 +I + V++M G+IV GG EL +E G+ + Sbjct: 202 FGHIDKEKLKVNIMRDGKIVMRGGSELISMIEEKGFKAI 240 >TM1417 [O] COG0396 ABC-type transport system involved in Fe-S cluster assembly, ATPase component Length = 245 Score = 97.1 bits (240), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 60/200 (30%), Positives = 101/200 (50%), Gaps = 14/200 (7%) Query: 41 IMGPNGTGKSTLASALMGHPKYEVTNGRVTXXXXXXXXXXXXXRARAGLFLAMQYPSEIA 100 I+G NG GKSTLA +MG Y T G++ RA+ G+ L Q P+ Sbjct: 34 ILGTNGAGKSTLAYLIMGLEGYRPTRGKILLDGEDITDLSVTERAKRGITLMWQEPARFT 93 Query: 101 GVTNSDFLRSAVNARRGEGNEVSLIRFIRQMESKMKGLEMNPEFSHRXXXXXXEGFSGGE 160 G+ D+L + RR E ++E ++ + +NP E SGGE Sbjct: 94 GIKIRDYL--TLGGRRKVSKE--------ELERVLELVGLNPALY--LERNVDEKLSGGE 141 Query: 161 KKRNEILQMMMLEPKFVILDEVDSGLDIDALRIVANGVNELRSPDRGFLIITHYQRLLNY 220 +KR E+ +++++P++ ILDE DSG+DI +L ++ ++EL S +++TH + + Sbjct: 142 RKRVELASILLMKPRYTILDEPDSGIDIMSLEMIEKVLSELVSAGGSVILVTHREEI--A 199 Query: 221 ITPDFVHVMMQGRIVKSGGP 240 + D+ ++ G I+K G P Sbjct: 200 LESDYAFLICHGSILKEGNP 219 >MA4406 [O] COG0396 ABC-type transport system involved in Fe-S cluster assembly, ATPase component Length = 247 Score = 96.7 bits (239), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 67/223 (30%), Positives = 106/223 (47%), Gaps = 29/223 (13%) Query: 41 IMGPNGTGKSTLASALMGHPKYEVTNGRVTXXXXXXXXXXXXXRARAGLFLAMQYPSEIA 100 + GPNG GKS L +MG +Y V GR+ RAR GL + MQ P ++A Sbjct: 32 LFGPNGAGKSALMRTIMGFSEYRVVKGRILFKGEDITHLPIDERARLGLGIMMQRPPDMA 91 Query: 101 GVTNSDFLRSAVNARRGEGNEVSLIRFIRQMESKMKGLEMNPEFSHRXXXXXXEGFSGGE 160 G+ D ++ A ++ E+ + L+M F R GFSGGE Sbjct: 92 GIKLKDLVKVASKGKKDP-------------ETLAENLDMK-RFLDRDVNV---GFSGGE 134 Query: 161 KKRNEILQMMMLEPKFVILDEVDSGLDIDALRIVANGVNE-----LRSP------DRGFL 209 KR+E+LQ+ P +LDE +SG+D+ ++ V + E L P + L Sbjct: 135 IKRSELLQLSAQNPSLYLLDEPESGVDLVSIEQVGMTIKELLEEGLECPGERCKKGKSAL 194 Query: 210 IITHYQRLLNYITPDFVHVMMQGRIVKSGGP-ELAHRLEADGY 251 IITH ++L+Y+ D +++ G ++ SG P ++ ++ GY Sbjct: 195 IITHTGQVLDYVQADRGYILCNGTVMCSGNPMKMLEEIKNKGY 237 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,406,365 Number of extensions: 583670 Number of successful extensions: 15788 Number of sequences better than 1.0e-05: 97 Number of HSP's gapped: 16200 Number of HSP's successfully gapped: 97 Length of query: 269 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 164 Effective length of database: 26,268,361 Effective search space: 4308011204 Effective search space used: 4308011204 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)