BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0427.0008.p (411 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value yeaN [P] COG2807 Cyanate permease 270 3e-72 ZyeaN [P] COG2807 Cyanate permease 270 3e-72 ECs2500 [P] COG2807 Cyanate permease 270 3e-72 ZyeaN [P] COG2807 Cyanate permease 269 5e-72 PA1626 [P] COG2807 Cyanate permease 228 1e-59 >yeaN [P] COG2807 Cyanate permease Length = 393 Score = 270 bits (690), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 161/394 (40%), Positives = 230/394 (58%), Gaps = 6/394 (1%) Query: 15 STTGIRKQARIWFMIAGIIFIAANLRAPLTSVGPLVGLIRENVHISNTLAGLITTVPLIA 74 +T + + RI +IAGI+ IA LR T PL+ IR ++ GL+TT+PL+A Sbjct: 3 CSTSLSGKNRI-VLIAGILMIATTLRVTFTGAAPLLDTIRSAYSLTTAQTGLLTTLPLLA 61 Query: 75 FALLSPFVPKLGRRYGVERIMLISLILLVIGIAVRSLSGAVTLFVGTAVLGFAITICNVL 134 FAL+SP + RR+G+ER + +L+L+ GIA+RSL LF GTAV+G I + NVL Sbjct: 62 FALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPYLLFGGTAVIGGGIALGNVL 121 Query: 135 LPSLIKREFPLQMGTMTGVYSVSMNLSGAIASGISVPLAVGAGLNWQGALGVWGILSVIS 194 LP LIKR+FP + +TG YS++M + A+ S + VPLA+ G WQGAL + +++ Sbjct: 122 LPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLALN-GFGWQGALLMLMCFPLLA 180 Query: 195 ILFWAPQLKXXXXXXXXXXXXXGNDHHVNIWRSPLAWQVTLFMGLQSTIFYVLVAWLPEI 254 + W PQ + H IWRSPLAWQVTLF+G+ S ++YV++ WLP I Sbjct: 181 LFLWLPQWRSQQHANLSTSRAL---HTRGIWRSPLAWQVTLFLGINSLVYYVIIGWLPAI 237 Query: 255 LKDQGISSSQSGWFLSIMIIASLPLSFIVPVIAGRMRNQRXXXXXXXXXXXXXXXXXXYG 314 L G S +Q+G ++ +A+ ++P+ +++QR + Sbjct: 238 LISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQRGIAAFVALMCAVGAVGLCFM 297 Query: 315 SIHLVLLWTIILGIGSGFAFGLSMMFFGLRTRSAHQAAELSGMAQSIGYLLAAMGPALIG 374 H + WT++ G GSG L + F GLR SAHQAA LSGMAQS+GYLLAA GP L+G Sbjct: 298 PAH-AITWTLLFGFGSGATMILGLTFIGLRASSAHQAAALSGMAQSVGYLLAACGPPLMG 356 Query: 375 YLHDATHSWSVPFLILACASALLGIIGLGAARNR 408 +HDA +WSVP + +A S L+ I GL A R++ Sbjct: 357 KIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDK 390 >ZyeaN [P] COG2807 Cyanate permease Length = 393 Score = 270 bits (690), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 161/394 (40%), Positives = 230/394 (58%), Gaps = 6/394 (1%) Query: 15 STTGIRKQARIWFMIAGIIFIAANLRAPLTSVGPLVGLIRENVHISNTLAGLITTVPLIA 74 +T + + RI +IAGI+ IA LR T PL+ IR ++ GL+TT+PL+A Sbjct: 3 CSTSLSGKNRI-VLIAGILMIATTLRVTFTGAAPLLDTIRSAYSLTTAQTGLLTTLPLLA 61 Query: 75 FALLSPFVPKLGRRYGVERIMLISLILLVIGIAVRSLSGAVTLFVGTAVLGFAITICNVL 134 FAL+SP + RR+G+ER + +L+L+ GIA+RSL LF GTAV+G I + NVL Sbjct: 62 FALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPYLLFGGTAVIGGGIALGNVL 121 Query: 135 LPSLIKREFPLQMGTMTGVYSVSMNLSGAIASGISVPLAVGAGLNWQGALGVWGILSVIS 194 LP LIKR+FP + +TG YS++M + A+ S + VPLA+ G WQGAL + +++ Sbjct: 122 LPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLALN-GFGWQGALLMLMCFPLLA 180 Query: 195 ILFWAPQLKXXXXXXXXXXXXXGNDHHVNIWRSPLAWQVTLFMGLQSTIFYVLVAWLPEI 254 + W PQ + H IWRSPLAWQVTLF+G+ S ++YV++ WLP I Sbjct: 181 LFLWLPQWRSQQHANLSTSRAL---HTRGIWRSPLAWQVTLFLGINSLVYYVIIGWLPAI 237 Query: 255 LKDQGISSSQSGWFLSIMIIASLPLSFIVPVIAGRMRNQRXXXXXXXXXXXXXXXXXXYG 314 L G S +Q+G ++ +A+ ++P+ +++QR + Sbjct: 238 LISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQRGIAAFVALMCAVGAVGLCFM 297 Query: 315 SIHLVLLWTIILGIGSGFAFGLSMMFFGLRTRSAHQAAELSGMAQSIGYLLAAMGPALIG 374 H + WT++ G GSG L + F GLR SAHQAA LSGMAQS+GYLLAA GP L+G Sbjct: 298 PAH-AITWTLLFGFGSGATMILGLTFIGLRASSAHQAAALSGMAQSVGYLLAACGPPLMG 356 Query: 375 YLHDATHSWSVPFLILACASALLGIIGLGAARNR 408 +HDA +WSVP + +A S L+ I GL A R++ Sbjct: 357 KIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDK 390 >ECs2500 [P] COG2807 Cyanate permease Length = 393 Score = 270 bits (690), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 161/394 (40%), Positives = 229/394 (58%), Gaps = 6/394 (1%) Query: 15 STTGIRKQARIWFMIAGIIFIAANLRAPLTSVGPLVGLIRENVHISNTLAGLITTVPLIA 74 +T + + RI +IAGI+ IA LR T PL+ IR ++ GL+TT+PL+A Sbjct: 3 CSTSLSGKNRI-VLIAGILMIATTLRVTFTGAAPLLDTIRSAYSLTTAQTGLLTTLPLLA 61 Query: 75 FALLSPFVPKLGRRYGVERIMLISLILLVIGIAVRSLSGAVTLFVGTAVLGFAITICNVL 134 FAL+SP + RR+G+ER + +L+L+ GIA+RSL LF GTAV+G I + NVL Sbjct: 62 FALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPYLLFGGTAVIGGGIALGNVL 121 Query: 135 LPSLIKREFPLQMGTMTGVYSVSMNLSGAIASGISVPLAVGAGLNWQGALGVWGILSVIS 194 LP LIKR+FP + +TG YS++M + A+ S + VPLA+ G WQGAL + +++ Sbjct: 122 LPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLALN-GFGWQGALLMLMCFPLLA 180 Query: 195 ILFWAPQLKXXXXXXXXXXXXXGNDHHVNIWRSPLAWQVTLFMGLQSTIFYVLVAWLPEI 254 + W PQ + H IWRSPLAWQVTLF+G+ S ++YV++ WLP I Sbjct: 181 LFLWLPQWRSQQHANLSTSRAL---HTRGIWRSPLAWQVTLFLGINSLVYYVIIGWLPAI 237 Query: 255 LKDQGISSSQSGWFLSIMIIASLPLSFIVPVIAGRMRNQRXXXXXXXXXXXXXXXXXXYG 314 L G S +Q+G ++ +A+ ++P+ +++QR + Sbjct: 238 LISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQRGIAAFVALMCAVGAVGLCFM 297 Query: 315 SIHLVLLWTIILGIGSGFAFGLSMMFFGLRTRSAHQAAELSGMAQSIGYLLAAMGPALIG 374 H + WT++ G GSG L + F GLR SAHQAA LSGMAQS+GYLLAA GP L+G Sbjct: 298 PAH-AITWTLLFGFGSGATMILGLTFIGLRASSAHQAAALSGMAQSVGYLLAACGPPLMG 356 Query: 375 YLHDATHSWSVPFLILACASALLGIIGLGAARNR 408 +HDA WSVP + +A S L+ I GL A R++ Sbjct: 357 KIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDK 390 Score = 269 bits (688), Expect = 5e-72, Method: Compositional matrix adjust. Identities = 158/381 (41%), Positives = 224/381 (58%), Gaps = 5/381 (1%) Query: 28 MIAGIIFIAANLRAPLTSVGPLVGLIRENVHISNTLAGLITTVPLIAFALLSPFVPKLGR 87 +IAGI+ IA LR T PL+ IR ++ GL+TT+PL+AFAL+SP + R Sbjct: 15 LIAGILMIATTLRVTFTGAAPLLDTIRSAYSLTTAQTGLLTTLPLLAFALISPLAAPVAR 74 Query: 88 RYGVERIMLISLILLVIGIAVRSLSGAVTLFVGTAVLGFAITICNVLLPSLIKREFPLQM 147 R+G+ER + +L+L+ GIA+RSL LF GTAV+G I + NVLLP LIKR+FP + Sbjct: 75 RFGMERSLFAALLLICAGIAIRSLPSPYLLFGGTAVIGGGIALGNVLLPGLIKRDFPHSV 134 Query: 148 GTMTGVYSVSMNLSGAIASGISVPLAVGAGLNWQGALGVWGILSVISILFWAPQLKXXXX 207 +TG YS++M + A+ S + VPLA+ G WQGAL + ++++ W PQ + Sbjct: 135 ARLTGAYSLTMGAAAALGSAMVVPLALN-GFGWQGALLMLMCFPLLALFLWLPQWRSQQH 193 Query: 208 XXXXXXXXXGNDHHVNIWRSPLAWQVTLFMGLQSTIFYVLVAWLPEILKDQGISSSQSGW 267 H IWRSPLAWQVTLF+G+ S ++YV++ WLP IL G S +Q+G Sbjct: 194 ANLSTSRAL---HTRGIWRSPLAWQVTLFLGINSLVYYVIIGWLPAILISHGYSEAQAGS 250 Query: 268 FLSIMIIASLPLSFIVPVIAGRMRNQRXXXXXXXXXXXXXXXXXXYGSIHLVLLWTIILG 327 ++ +A+ ++P+ +++QR + H + WT++ G Sbjct: 251 LHGLLQLATAAPGLLIPLFLHHVKDQRGIAAFVALMCAVGAVGLCFMPAH-AITWTLLFG 309 Query: 328 IGSGFAFGLSMMFFGLRTRSAHQAAELSGMAQSIGYLLAAMGPALIGYLHDATHSWSVPF 387 GSG L + F GLR SAHQAA LSGMAQS+GYLLAA GP L+G +HDA +WSVP Sbjct: 310 FGSGATMILGLTFIGLRASSAHQAAALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPL 369 Query: 388 LILACASALLGIIGLGAARNR 408 + +A S L+ I GL A R++ Sbjct: 370 MGVAILSLLMAIFGLCAGRDK 390 >PA1626 [P] COG2807 Cyanate permease Length = 424 Score = 228 bits (581), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 141/377 (37%), Positives = 215/377 (57%), Gaps = 19/377 (5%) Query: 40 RAPLTSVGPLVGLIRENVHISNTLAGLITTVPLIAFALLSPFVPKLGRRYGVERIMLISL 99 R L+S+ PL+ +R++ +S AGL+TT P++ L +P P+L RR G ER +L+ L Sbjct: 54 RPALSSLAPLLNQVRDDTGLSAAAAGLLTTAPVLCLGLFAPLAPRLARRIGAERSVLLIL 113 Query: 100 ILLVIGIAVRSLSGAVTLFVGTAVLGFAITICNVLLPSLIKREFPLQMGTMTGVYSVSMN 159 L GI +RSL LF G+ + G +I I VLLP ++KR+FP Q G MTGVY++++ Sbjct: 114 FCLAGGIVLRSLFPVFGLFAGSVLAGASIGIIGVLLPGIVKRDFPRQAGVMTGVYTMALC 173 Query: 160 LSGAIASGISVPLAVGAGLNWQGALGVWGILSVISILFWAPQLKXXXXXXXXXXXXXGND 219 L A+A+G + PL G NW+ AL W + ++++ FW PQ + G Sbjct: 174 LGAALAAGATAPLEKLFG-NWELALAFWSLPAILAAAFWLPQTR------------QGQH 220 Query: 220 HH------VNIWRSPLAWQVTLFMGLQSTIFYVLVAWLPEILKDQGISSSQSGWFLSIMI 273 H + +WR PLAWQVTL+MGLQS++ Y++ WLP IL D+G+++ ++G LS + Sbjct: 221 AHRQAFRVIGLWRDPLAWQVTLYMGLQSSLAYIVFGWLPSILIDRGMTAVEAGLVLSGSV 280 Query: 274 IASLPLSFIVPVIAGRMRNQRXXXXXXXXXXXXXXXXXXYGSIHLVLLWTIILGIGSGFA 333 + L + + P +A R R+QR Y + + W ++LG+G G Sbjct: 281 MLQLITALLAPWLAARARDQRLAVVLVMATTLAGLLGFLYAPLQTIWGWAVLLGLGQGGT 340 Query: 334 FGLSMMFFGLRTRSAHQAAELSGMAQSIGYLLAAMGPALIGYLHDATHSWSVPFLILACA 393 F +++ LR+R AH A+ LSGMAQ +GY LAAMGP ++G +HD T W+ I Sbjct: 341 FSIALALIVLRSRDAHVASHLSGMAQGVGYTLAAMGPFMVGVVHDLTGGWNAVGYIFIGV 400 Query: 394 SALLGIIGLGAARNRFV 410 + + GLGA R+++V Sbjct: 401 AIAATLFGLGAGRSQYV 417 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.141 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,223,675 Number of extensions: 1070507 Number of successful extensions: 4572 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 4532 Number of HSP's successfully gapped: 18 Length of query: 411 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 302 Effective length of database: 25,807,025 Effective search space: 7793721550 Effective search space used: 7793721550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 117 (49.7 bits)