BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0427.0005.p (521 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2115 [G] COG1653 ABC-type sugar transport system, periplasmic... 125 1e-28 BH0796 [G] COG1653 ABC-type sugar transport system, periplasmic ... 119 1e-26 SP0092 [G] COG1653 ABC-type sugar transport system, periplasmic ... 90 7e-18 SPy1592 [G] COG1653 ABC-type sugar transport system, periplasmic... 72 2e-12 >lin2115 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 485 Score = 125 bits (315), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 143/539 (26%), Positives = 235/539 (43%), Gaps = 76/539 (14%) Query: 1 MKKSYVILLAVVLLFSTLIGCSQGSNSTNTADKEDNSSAAKPENNGKPAETVKIKYLVPG 60 MKK + +++A + L L C GS+ A+ E +K+ + G Sbjct: 1 MKKKWGVIIASLCLLLVLGAC--GSDEKADAN-----------------EVPTLKWYMIG 41 Query: 61 TEPKDYKEVFQKVNEKLAADGVGVEVEKIYIPWDAWDQKLNLMLSTGEDFDLFHVMQDRT 120 T KD +EV ++VN K + +GV+VE I W + +++ ++++GE+FD+ + Sbjct: 42 TPQKDTEEVMKEVN-KYTEEKIGVKVEMTQIDWGDYGKRMQTVINSGENFDIAYSAAGE- 99 Query: 121 PFSSYYNRGALADITDAIEKYGQNLKKHIPDDIFDGAKIDGKYYAAPSYWVEMASEGQFD 180 F SY +GA + ++K G+ +K + + +++GA IDG Y PS E+ + + Sbjct: 100 -FVSYSQKGAFLPLNKYLDKEGKKMKAELNEVLWEGATIDGNIYGVPSN-KEVGEQQVYV 157 Query: 181 IRRDILRENNLKEPTTPAELISAWETVMKNWKGSNKPYL---GTRADFDPIFLHTSILHR 237 ++++ + K + + S E ++K K N+P + G DF P F + ++ Sbjct: 158 FNKNLV--DKYKMDISKVKNFSDLEPMLKTIK-ENEPSITPIGGNKDFKPAFSYDYLIDN 214 Query: 238 TYDTFPFTVKDKFFYVNQNGEVKSWIETEEFKKDASFMRELYTKGITNPDILVMKQEQVD 297 PF V G++ ++ E + + + Y KG DI Sbjct: 215 AV-PLPFAVNQ----YEDTGKIVNFYEQPDTLETLKSLHSFYQKGYVTKDIATSTDPW-- 267 Query: 298 AQLDSGDWFVRXXXXXXXXXXKKYNPEA-------TVNDIGVVWFNPEKEYLRPLTFKNG 350 D +WFVR +KY P A T VV P E P+ N Sbjct: 268 -SYDKENWFVRI---------EKYQPYAENIWDKNTGGKYKVV-TQPIAE--TPIIKNNS 314 Query: 351 -----NAVPVNSKHPEAAVKFMDWMLASQENYDLVQYGIEGKHYTK--DGEKGYKPIKDX 403 AV V S+HPE AVKF+D + + +LV GIEG HY + DG+ P + Sbjct: 315 VTGAIQAVSVTSQHPEEAVKFLDLLNTDEYLRNLVDKGIEGTHYKELDDGKIQDLPAR-- 372 Query: 404 XXXXXXGYRGSDSQNGNVNFMRFDLENSIPDNNKVLFEPNPNAVNSIAANFIFDPTNVRA 463 Y GN +F+ E+ D N +V S F FD + VR Sbjct: 373 ----VERYSMPTYALGN-HFILKLYEDEPADKWDQFKTFNKKSVASPGLGFYFDSSKVRT 427 Query: 464 E---YTNIMSEASASITPIYMGVLEYDKAFPAALEKMRKAGLDKVVAEYQKQFQEYQAS 519 E TN+ +E + ++ + +EY + F A K+ AG+ KV+ E QKQ+ EY+A+ Sbjct: 428 EIASITNVCNEFAPALLTGTVDPVEYTEKFKA---KLNDAGMKKVMKEMQKQYDEYRAT 483 >BH0796 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 500 Score = 119 bits (298), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 130/529 (24%), Positives = 227/529 (42%), Gaps = 48/529 (9%) Query: 3 KSYVILLAV-VLLFSTLIGCSQGSNSTNTADKEDNSSAAKPENNGKPAET--VKIKYLVP 59 KS+V+ LAV V L L+ CS + S+ + + + S + N + + V + + + Sbjct: 2 KSFVMKLAVMVFLLVGLVACSSANESSQSEPEGHDESGEQSANETEATDLDHVTLTWYMI 61 Query: 60 GTEPKDYKEVFQKVNEKLAADGVGVEVEKIYIPWDAWDQKLNLMLSTGEDFDLFHVMQDR 119 GT D + V ++VN + + V+ + W +D+++ ++ ++GE +D+ Sbjct: 62 GTPQPDLELVMEEVN-AYTEEKINATVDLRMLDWGEYDERMQVITTSGEAYDIAFTSSWA 120 Query: 120 TPFSSYYNRGALADITDAIEKYGQNLKKHIPDDIFDGAKIDGKYYAAPSYWVEMASEGQF 179 ++ RGA ++ D ++++GQ +K+ I +GA++DGK YA P+ E+ + Sbjct: 121 NNYALNARRGAFLELNDLLDEHGQEMKELIDPAFLEGAQVDGKLYAVPTN-KEVGQQAVL 179 Query: 180 DIRRDILRENNLKEPTTPAEL-ISAWETVMKNWKGSNKPYLGTRADFDPIFLHTSILHRT 238 +++ ++NL + + + V+K + P A FD SIL Sbjct: 180 SFNNELVEKHNLDLSSVHSLADLEPLLAVIKEEESDVTPI----ATFDAYLPFDSILQ-- 233 Query: 239 YDTFPFTVKDKFFYVNQNGEVKSWIETEEFKKDASFMRELYTKGITNPDILVMKQEQVDA 298 + PF F EV + E + + M + Y KG PD D+ Sbjct: 234 -EEMPFA----FRLEGNTNEVINKYEEDITMETLKTMHDYYKKGYIRPDAAT----STDS 284 Query: 299 -QLDSGDWFVRXXXXXXXXXXKKYNPEATVNDIGVVWFNPEKEYLR-PLTFKNG-----N 351 L++ +WFVR + Y P A + + L P F N Sbjct: 285 WPLETPNWFVR---------KELYQPYAELIWTRTAGYEIATRPLHEPYIFNNSVTGSMQ 335 Query: 352 AVPVNSKHPEAAVKFMDWMLASQENYDLVQYGIEGKHY--TKDGEKGYKPIKDXXXXXXX 409 A+ SK+PE A+ F++ + + +L+ GIEG HY +DG P + Sbjct: 336 AISATSKNPERAMMFLNLLNSDPYLRNLLDKGIEGVHYEELEDGTINDLPAR------VE 389 Query: 410 GYRGSDSQNGNVNFMRFDLENSIPDNNKVLFEP-NPNAVNSIAANFIFDPTNVRAEYTNI 468 Y GN ++ L P + FE N +A+ S A F FD VR E I Sbjct: 390 RYNMPSFAIGNQLILK--LYEDDPQDKWEAFEAFNESAIPSPALGFYFDSNPVRTEIAAI 447 Query: 469 MSEASASITPIYMGVLEYDKAFPAALEKMRKAGLDKVVAEYQKQFQEYQ 517 + S + G ++ ++ P +K+ +AGL KV+ E Q+QF E+Q Sbjct: 448 SNVTSEFSPALLKGAVDPEEYLPLFNDKLNEAGLQKVIDEMQRQFDEWQ 496 >SP0092 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 491 Score = 90.1 bits (222), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 117/484 (24%), Positives = 214/484 (44%), Gaps = 61/484 (12%) Query: 54 IKYLVPGTEPKDYKEVFQKVNEKLAADGVGVEVEKIYIPWDAWDQKLNLMLSTGEDFDLF 113 IK G +P + E+ N K+ + VG +++ Y+ W + +K++++ S+GE++D+ Sbjct: 43 IKMYQIGDKPDNLDELLANAN-KIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDI- 100 Query: 114 HVMQDRTPFSSYY----NRGALADITDAIEKYGQNLKKHIPDDIFDGAKIDGKYYAAPSY 169 F+ Y +GA AD+T+ +K G++L K + G ++GK YA P Sbjct: 101 -------AFADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVP-V 152 Query: 170 WVEMASEGQFDIRRDILRENNLKEPTTPAELISAWETVMKNWKGSNKPYLGTRADFDPIF 229 +AS F +L + + ++++ET+ +P L + P Sbjct: 153 AANVASSQNFAFNGTLLAKYGID-----ISGVTSYETL--------EPVLKQIKEKAPDV 199 Query: 230 LHTSI--LHRTYDTFPFTVKDKF-FYVNQNGEVKSWI---ETEEFKKDASFMRELYTKGI 283 + +I + D F + V + F ++ G+ + E FK+ + + Y G Sbjct: 200 VPFAIGKVFIPSDNFDYPVANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGY 259 Query: 284 TNPDILVMKQEQVDAQLDSGDWFVRXXXXXXXXXXKKYNPEATVNDIGVVWFNPEKEYLR 343 D+ D Q D+ WFVR DI + P +++ Sbjct: 260 IPKDV-ATSDTSFDLQQDT--WFVREETVGPADYGNSLLSRVANKDIQI---KPITNFIK 313 Query: 344 P-LTFKNGN-AVPVNSKHPEAAVKFMDWMLASQENYDLVQYGIEGKHYTK-DGEKGYKPI 400 T + N + NSK+ E +++ ++ + + E + + YG EGK++ K +G++ + Sbjct: 314 KNQTTQVANFVISNNSKNKEKSMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRV 373 Query: 401 KDXXXXXXXGYRGSDSQNG---NVNFMRFDLEN----SIPDNNKVLFEPNPNAVNSIAAN 453 D GY+G+ G N++ + EN I ++ K L E A S A Sbjct: 374 LD-------GYKGNTHMGGWNTGNNWILYINENVTDQQIENSKKELAE----AKESPALG 422 Query: 454 FIFDPTNVRAEYTNIMSEASASITPIYMGVLEYDKAFPAALEKMRKAGL-DKVVAEYQKQ 512 FIF+ NV++E + I + T I G ++ DKA P +EK++ G +KV+ E QKQ Sbjct: 423 FIFNTDNVKSEISAIANTMQQFDTAINTGTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQ 482 Query: 513 FQEY 516 + E+ Sbjct: 483 YDEF 486 >SPy1592 [G] COG1653 ABC-type sugar transport system, periplasmic component Length = 380 Score = 72.4 bits (176), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 99/413 (23%), Positives = 161/413 (38%), Gaps = 94/413 (22%) Query: 28 TNTADKEDNSSAAKPENNGKPAETVKIKYLVPGTEPKDYKEVFQKVNEKLAADGVGVEVE 87 T T N +A + ++ K ++ + Y V G +P ++ E+ N+++ + G V+ Sbjct: 11 TGTVLATSNLAACESKSASKDSDVKLLMYQV-GDKPDNFDELMTIANKRIK-EKTGATVD 68 Query: 88 KIYIPWDAWDQKLNLMLSTGEDFDLFHVMQDRTPFSSYY----NRGALADITDAIEKYGQ 143 YI W WD K++ ++++GE++D+ F++ Y +GA AD+T + KY + Sbjct: 69 LQYIGWGDWDDKMSTIIASGENYDI--------AFANNYVVNAQKGAFADLTTLMPKYAK 120 Query: 144 NLKKHIPDDIFDGAKIDGKYYAAP-----------SYWVEMASEGQFDIR--------RD 184 K++ G IDGK YA P S+ E+ + DI + Sbjct: 121 KTYKNLDPAYIKGNTIDGKLYAFPVDANVYAQQMLSFNKELVDKYGLDISNIKSYADAEN 180 Query: 185 ILRENNLKEPTTPAELISAWETVMKNWKGSNKPYLGTRADFDPIFLHTSILHRTYDTFPF 244 +L++ + KEP T A I D+D T PF Sbjct: 181 VLKQFHEKEPNTAAFAIGQ--------------VFSMSGDYDYPLTKTQ---------PF 217 Query: 245 TVK---DKFFYVNQNGEVKSWIETEEFKKDASFMRELYTKGITNPDILVMKQEQVDAQLD 301 VK K +NQ E E FK + M + Y +G+ D + L+ Sbjct: 218 AVKIDEGKPTIINQ-------YEDESFKNNLRLMHKWYKEGLIPTDAATNTE---GYPLE 267 Query: 302 SGDWFVRXXXXXXXXXXKKYNPEATVNDIGVVWFNPEKEYLRPLT--FKNGN-------A 352 WF+R A DI RPLT K + Sbjct: 268 GNTWFMREETQGPMDYGDTILTNAAGKDI----------VSRPLTKPLKTTSQAQMANFV 317 Query: 353 VPVNSKHPEAAVKFMDWMLASQENYDLVQYGIEGKHYTKDGEK------GYKP 399 V SK+ E AV+ + + + E + + YG+EGK + K G+K GY+P Sbjct: 318 VSSVSKNKEKAVEVLSLLNSDPELLNGLVYGVEGKAWEKIGDKKIKLLDGYQP 370 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.315 0.133 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,185,401 Number of extensions: 1636738 Number of successful extensions: 2522 Number of sequences better than 1.0e-05: 5 Number of HSP's gapped: 2517 Number of HSP's successfully gapped: 5 Length of query: 521 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 410 Effective length of database: 25,576,357 Effective search space: 10486306370 Effective search space used: 10486306370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 118 (50.1 bits)