BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0422.0019.p (418 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM0146 [V] COG3023 Negative regulator of beta-lactamase expression 498 e-140 aq_1928 [J] COG0050 GTPases - translation elongation factors 106 7e-23 CAC1824 [O] COG0542 ATPases with chaperone activity, ATP-binding... 57 8e-08 lin0264 [O] COG0542 ATPases with chaperone activity, ATP-binding... 56 9e-08 L0222 [O] COG0542 ATPases with chaperone activity, ATP-binding s... 55 2e-07 >STM0146 [V] COG3023 Negative regulator of beta-lactamase expression Length = 252 Score = 498 bits (1281), Expect = e-140, Method: Compositional matrix adjust. Identities = 0/386 (0%), Positives = 0/386 (0%), Gaps = 1/386 (0%) Query: 13 CSFCGKSQEQVRKLVAGPG-VYICDECIELCTXXXXXXXXXXXXXXXKDIPKPKEIRDIL 71 S + + + + + I Sbjct: 6 PSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGNSTALLQQRWPAARITGIDSSP 65 Query: 72 DQYVIGQEQAKKSLSVAVYNHYKRVNSQSKIEDVELTKSNILLLGPTGSGKTLLAQTMAK 131 + V + + + + L Sbjct: 66 AMIAEARSALPDCQFVEADIRNWQPVQALDLIFANASLQWLPDHYELFPHLVSLLNPQGV 125 Query: 132 IINVPFAIADATSLTEAGYVGEDVENILLKLIQAADYDVEKAERGIIYIDEIDKVARKSE 191 + + V + A + Sbjct: 126 LAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLAGVHAYYDILSEAGCEVDIWRTTYYH 185 Query: 192 NPSITRDVSGEGVQQALLKILEGTVASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGL 251 + + L L+ S +++ Q I+ Sbjct: 186 QMPSHQAIIDWVTATGLRPWLQDLTESEQQLFLKRYHQMLEEQYPLQENGQILLAFPRLF 245 Query: 252 EQMIKRRIGKKVIGFNAAVEGQKDLKTGEYLSMVLPEDLLKFGLIPEFVGRLPVISTLEP 311 Sbjct: 246 305 Query: 312 LDENTLVRILSEPKNALTKQYIKLLEMDNVALKFEPLALEAIAKEAIKRNTGARGLRAII 371 Sbjct: 306 365 Query: 372 ESIMLDVMYEVPSRDDIKDCVITEQV 397 Sbjct: 366 391 >aq_1928 [J] COG0050 GTPases - translation elongation factors Length = 450 Score = 106 bits (264), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 83/231 (35%), Positives = 114/231 (49%), Gaps = 58/231 (25%) Query: 159 LLKLIQAADYDVEKAER-GIIYIDEIDKVARKSENPSITRDVSGEGVQQALLKILEGTVA 217 L+ + + A + +AE GII+IDEIDK+A K+ P VS EGVQ+ LL ILEGT Sbjct: 240 LIDMEEVAREAIYRAENFGIIFIDEIDKIAVKT--PGAGPGVSREGVQRDLLPILEGTTV 297 Query: 218 SVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGLEQMIKRRIGKKVIGFNAAVEGQKDLK 277 + ++ + T +ILFI GAF Sbjct: 298 NT-----------KYGPVKTDHILFIGAGAF----------------------------- 317 Query: 278 TGEYLSMVLPEDLLKFGLIPEFVGRLPVISTLEPLDENTLVRILSEPKNALTKQYIKLLE 337 M P DL IPE GR P+ L+PL + RIL EP+NALTKQYI+LL+ Sbjct: 318 -----HMAKPSDL-----IPELQGRFPIRVELKPLTKEDFKRILVEPENALTKQYIELLK 367 Query: 338 MDNVALKFEPLALEAIAKEAIKRNT-----GARGLRAIIESIMLDVMYEVP 383 +NV L+F A+E IA+ A + NT GAR L ++E ++ D+ + P Sbjct: 368 TENVYLEFTDDAIEEIARIAEEINTKTENIGARRLHTVMEKLLEDISFNAP 418 Score = 92.4 bits (228), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 42/103 (40%), Positives = 72/103 (69%), Gaps = 1/103 (0%) Query: 64 PKEIRDILDQYVIGQEQAKKSLSVAVYNHYKRVNSQSKIEDVELTKSNILLLGPTGSGKT 123 PK + + L++YV+GQE+AKK++++A+ N ++R + + E+ NIL++GPTG GKT Sbjct: 15 PKRVVEELNKYVVGQEEAKKAVAIALRNRWRRQKLPENLRN-EVIPKNILMIGPTGVGKT 73 Query: 124 LLAQTMAKIINVPFAIADATSLTEAGYVGEDVENILLKLIQAA 166 +A+ +A +I PF +AT TE GYVG DVE+++ +L++ + Sbjct: 74 EIARRLANLIKAPFVKVEATKYTEIGYVGRDVESMVRELVEVS 116 >CAC1824 [O] COG0542 ATPases with chaperone activity, ATP-binding subunit Length = 750 Score = 56.6 bits (135), Expect = 8e-08, Method: Compositional matrix adjust. Identities = 76/316 (24%), Positives = 132/316 (41%), Gaps = 78/316 (24%) Query: 75 VIGQEQAKKSLSVAVYNHYKRVNSQSKIEDVELTKSNILLLGPTGSGKTLLAQTMAKIIN 134 + GQ++A +S+ ++ KR S + D ++ L +GPTG GKT +++ +A+ +N Sbjct: 462 IFGQDKALESIVQSI----KR--SSAGFNDENKPVASFLFVGPTGVGKTEISKQLAEALN 515 Query: 135 VPFAIADATSLTEA-----------GYVGEDVENILLKLIQAADYDVEKAERGIIYIDEI 183 + D + E GYVG + +L ++ Y I+ +DEI Sbjct: 516 IKLIRFDMSEYQEKHTVARLIGSPPGYVGYEEGGLLTDAVRKNPY-------CILLLDEI 568 Query: 184 DKVARKSENPSITRDVSGEGVQQALLKILEGTVASVPPQGGRKHPHQEFIQIDTTNILFI 243 +K + + LL++++ A++ G+K + I I T+N Sbjct: 569 EK--------------AHSDILNVLLQVMD--YATLTDNSGKKADFRNIILIMTSN---- 608 Query: 244 VGGAFDGLEQMIKRRIGKKVIGFNAAVEGQKDLKTGEYLSMVLPEDLLKFGLIPEFVGRL 303 GA +GK +GF V G+ ++ + +S + PEF RL Sbjct: 609 -AGA---------SEVGKNKVGFGNRVVGEDNID--KEVSKIFS---------PEFRNRL 647 Query: 304 PVISTLEPLDENTLVRI----LSEPKNALTKQYIKLLEMDNVALKFEPLALEAIAKEAIK 359 I +DE+ +R+ L E + L K++IK+ D + IA + + Sbjct: 648 DDIIIFNKMDEDMAIRVAKRSLDEFQQKLNKKHIKIKVTDE--------CYKWIASKGLN 699 Query: 360 RNTGARG-LRAIIESI 374 N GAR LR I E I Sbjct: 700 SNYGAREILRVIQEKI 715 >lin0264 [O] COG0542 ATPases with chaperone activity, ATP-binding subunit Length = 820 Score = 56.2 bits (134), Expect = 9e-08, Method: Compositional matrix adjust. Identities = 88/335 (26%), Positives = 147/335 (43%), Gaps = 67/335 (20%) Query: 70 ILDQYVIGQEQAKKSLSVAVYNHYKRVNSQSKIEDVELTKSNILLLGPTGSGKTLLAQTM 129 +L + VIGQ+ A K++S+AV +++ ++D + + + LGPTG GKT LA+ + Sbjct: 504 LLHERVIGQDAAVKAVSLAVRR------ARAGLKDPKRPIGSFIFLGPTGVGKTELARAL 557 Query: 130 AK--------IINV-------PFAIADATSLTEAGYVGEDVENILLKLIQAADYDVEKAE 174 A+ +I + F+ A GYVG + L + ++ Y V Sbjct: 558 AESMFGDEDSMIRIDMSEYMEKFSTARLVG-APPGYVGYEEGGQLTEKVRQKPYSV---- 612 Query: 175 RGIIYIDEIDKVARKSENPSITRDVSGEGVQQALLKILEGTVASVPPQGGRKHPHQEFIQ 234 + +DEI+K +P + LL++L+ + GR + + Sbjct: 613 ---VLLDEIEKA-----HPDVF---------NMLLQVLDD--GRLTDSKGRVVDFRNTVI 653 Query: 235 IDTTNILFIVGGAFDGLEQMIKRRIGKKVIGFNAAVEGQKDLKTGEYLSMVLPEDLLKFG 294 I T+NI G ++M + K +GFN ++ KD K E+ VL + LK Sbjct: 654 IMTSNI---------GAQEMKQ----DKSMGFNV-IDPLKDHKAMEH--RVLQD--LKQA 695 Query: 295 LIPEFVGRLPVISTLEPLDENTLVRILSEPKNALTKQYIKLLEMDNVALKFEPLALEAIA 354 PEF+ R+ L E L +I++ LT Q K L ++ +K A IA Sbjct: 696 FRPEFINRIDETIVFHSLQEKELKQIVT----LLTSQLTKRLAERDIHVKLTEGAKAKIA 751 Query: 355 KEAIKRNTGARGLRAIIESIMLDVMYEVPSRDDIK 389 K+ GAR L+ I+ + D++ E R +IK Sbjct: 752 KDGYDPEYGARPLKRAIQKEVEDMLSEELLRGNIK 786 >L0222 [O] COG0542 ATPases with chaperone activity, ATP-binding subunit Length = 816 Score = 55.5 bits (132), Expect = 2e-07, Method: Compositional matrix adjust. Identities = 90/331 (27%), Positives = 144/331 (43%), Gaps = 77/331 (23%) Query: 71 LDQYVIGQEQAKKSLSVAVYNHYKRVNSQSKIEDVELTKSNILLLGPTGSGKTLLAQTMA 130 L + V+GQE+A ++S A+ ++S + D + + LGPTG GKT LA+ +A Sbjct: 515 LHKRVVGQEEAISAVSRAIRR------ARSGVADSRRPMGSFMFLGPTGVGKTELAKALA 568 Query: 131 --------KIINV---PFAIADATSL---TEAGYVGEDVENILLKLIQAADYDVEKAERG 176 +I V F +TS GYVG D L + ++ Y V Sbjct: 569 DSVFGSEDNMIRVDMSEFMEKHSTSRLIGAPPGYVGYDEGGQLTERVRNKPYSV------ 622 Query: 177 IIYIDEIDKVARKSENPSITRDVSGEGVQQALLKILEGTVASVPPQGGRKHPHQEFIQID 236 + +DE++K +P V +L+IL+ V GRK + I I Sbjct: 623 -VLLDEVEKA-----HPD---------VFNIMLQILDDGF--VTDTKGRKVDFRNTIIIM 665 Query: 237 TTNILFIVGGAFDGLEQMIKRRIGKKVIGFNAAVEGQKDLKTGEYLSM---VLPEDLLKF 293 T+N+ GA + K +GF G K++ T +Y +M +L E LK Sbjct: 666 TSNL-----GATALRDD--------KTVGF-----GAKNI-TADYSAMKSRILEE--LKR 704 Query: 294 GLIPEFVGRLP---VISTLEPLDENTLVRILSEPKNALTKQYIKLLEMDNVALKFEPLAL 350 PEF+ R+ V +LE + +V+I+S K IK L ++ +K P A+ Sbjct: 705 HYRPEFLNRIDENIVFHSLESQEIEQIVKIMS-------KSLIKRLAEQDIHVKLTPSAV 757 Query: 351 EAIAKEAIKRNTGARGLRAIIESIMLDVMYE 381 + IA+ GAR LR ++ + D++ E Sbjct: 758 KLIAEVGFDPEYGARPLRKALQKEVEDLLSE 788 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.137 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,105,888 Number of extensions: 1110040 Number of successful extensions: 6002 Number of sequences better than 1.0e-05: 23 Number of HSP's gapped: 6119 Number of HSP's successfully gapped: 25 Length of query: 418 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 309 Effective length of database: 25,807,025 Effective search space: 7974370725 Effective search space used: 7974370725 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)