BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0422.0016.p (432 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC2638 [O] COG1067 Predicted ATP-dependent protease 466 e-131 CAC3716 [O] COG1067 Predicted ATP-dependent protease 281 2e-75 PH0452_3 [O] COG1067 Predicted ATP-dependent protease 60 5e-09 MA0669 [L] COG0470 ATPase involved in DNA replication 58 3e-08 SMc01045 [T] COG2204 Response regulator containing CheY-like rec... 57 5e-08 >CAC2638 [O] COG1067 Predicted ATP-dependent protease Length = 566 Score = 466 bits (1199), Expect = e-131, Method: Compositional matrix adjust. Identities = 206/384 (53%), Positives = 289/384 (75%) Query: 6 LLMIVQLFFALVIGVYFWNLLRGQKTNKTAVDRESRKELDKLRKMRMISLTKPLSEKTRP 65 +++IV++ F ++IG+YF+ L+ Q+ + ++D+E++KEL+KL MR I L+ PL E++RP Sbjct: 4 VIIIVEMIFNIIIGIYFFYALKNQQFGRISIDKENKKELEKLNDMRKIRLSIPLCEESRP 63 Query: 66 ASIGDIVGQKDGLRALKAALCSANPQHVIIYGPPGVGKTAAARVVMEEAKKNGLSPFKSD 125 S +I+GQK+G++ LKA++C NPQHVIIYGPPGVGKTAAAR+ +E AK SPF Sbjct: 64 KSFDEIIGQKNGIKTLKASICGPNPQHVIIYGPPGVGKTAAARLALEYAKTCAFSPFNDK 123 Query: 126 AKFTEIDATTARFDERGIADPLIGSVHDPIYQGAGAMGVAGVPQPKPGAVTKAHGGILFL 185 AKF EIDATT RFDERGIADPLIGSVHDPIYQGAG G AG+PQPK GAV++AHGG+LF+ Sbjct: 124 AKFVEIDATTLRFDERGIADPLIGSVHDPIYQGAGKFGNAGIPQPKMGAVSRAHGGVLFI 183 Query: 186 DEIGELHPIQMNKLLKVLEDRKVLLESAYYNSEDSNTPAYIHDIFQNGLPADFRLVGATT 245 DEIGEL+PI+MNKLLKV+EDRKV +SAYYNS N P YI ++F+ G PADFRL+GATT Sbjct: 184 DEIGELNPIEMNKLLKVMEDRKVFFDSAYYNSSSENMPEYIREVFEEGFPADFRLIGATT 243 Query: 246 RSPEEISPALRSRCMEIYFRPLLPEEIAVIGRDAVQKIGLKPSPEAIEVVQQYATNGREA 305 ++PEEI+PA+RSRC+EI+FR L EEI +I ++AV+KI + +A+E++ Y++NGR+ Sbjct: 244 KNPEEINPAIRSRCIEIFFRALDAEEIKIIAKNAVEKINVNIDSKALELIAMYSSNGRDV 303 Query: 306 VNMIQLAAGLALTEGRDTLXXXXXXXXXXXXQLPMRTERKIPSSPQIGLVNGLAVYGPGM 365 +N+IQLA G+ + E R + + +R+ +K+ S +IG VNGL V+G Sbjct: 304 INLIQLACGIVINENRGRIEAEDILWIAENGRYSLRSYKKLSSVSRIGCVNGLGVFGIDN 363 Query: 366 GTLLEIEVSAAPAKSGQGRLNVTG 389 G ++++E +A ++ G++ +TG Sbjct: 364 GIVMDVEATAVRVENSVGQIKITG 387 >CAC3716 [O] COG1067 Predicted ATP-dependent protease Length = 634 Score = 281 bits (718), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 152/336 (45%), Positives = 204/336 (60%), Gaps = 11/336 (3%) Query: 64 RPASIGDIVGQKDGLRALKAALCSANPQHVIIYGPPGVGKTAAARVVMEEAKKNGLSPFK 123 RP + +IVGQ+ ++AL + L S PQH+I+YGPPGVGKT AAR+ +EE KK +PFK Sbjct: 168 RPQTFSEIVGQERAIKALVSKLASPYPQHIILYGPPGVGKTTAARIALEEVKKLKYTPFK 227 Query: 124 SDAKFTEIDATTARFDERGIADPLIGSVHDPIYQGAG-AMGVAGVPQPKPGAVTKAHGGI 182 DA F E+D T R+D R I +PL+GSVHDPIYQG+ + GVP+PK G VT AHGG+ Sbjct: 228 KDANFVEVDGATLRWDPREITNPLLGSVHDPIYQGSKRDLAETGVPEPKSGLVTDAHGGV 287 Query: 183 LFLDEIGELHPIQMNKLLKVLEDRKVLLESAYYNSEDSNTPAYIHDIFQNGLPADFRLVG 242 LF+DEIGEL + NKLLKVLED+KV S+YY+ +D N P YI F+ G PADF L+G Sbjct: 288 LFIDEIGELDVMLQNKLLKVLEDKKVEFSSSYYDPDDENVPKYIKYFFEKGAPADFVLIG 347 Query: 243 ATTRSPEEISPALRSRCMEIYFRPLLPEEIAVIGRDAVQKIGLKPSPEAIEVVQQYATNG 302 ATT+ P EI+ ALRSRC E+YF PL +I I +A K+ + E++ +Y G Sbjct: 348 ATTKDPSEINSALRSRCTEVYFEPLSSSDIMDIIDNAADKLNVLLEDGVKELISRYTIQG 407 Query: 303 REAVNMIQLAAGLAL-TEGRD-----TLXXXXXXXXXXXXQLPMRTERKIPSSPQIGLVN 356 R A+N++ G L ++G + + +L + + ++G V Sbjct: 408 RRAINILADVYGYVLYSQGLEPSDKVEIKVKDVETIVGISRLTPYEKNIFDAEYEVGHVF 467 Query: 357 GLAVYGPGMGTLLEIEVSAAPAK-SGQG--RLNVTG 389 GL V G MG+ +EIE A AK G+G R N T Sbjct: 468 GLGVSG-FMGSTIEIEAEAFEAKQKGKGTIRFNETA 502 >PH0452_3 [O] COG1067 Predicted ATP-dependent protease Length = 443 Score = 60.5 bits (145), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 73/272 (26%), Positives = 118/272 (43%), Gaps = 44/272 (16%) Query: 159 AGAMGVAGVPQPKPGAVTKAHGGILFLDEIGELHPIQMNKLLKVLEDRKVLLESAYYNSE 218 +G +G + +PG + +AH G+LF+DEI L LL ++++K + SE Sbjct: 1 SGGLGTPAHLRVEPGMIHRAHKGVLFIDEIATLSLKMQQSLLTAMQEKKFPITG---QSE 57 Query: 219 DSNTPAYIHDIFQNGLPADFRLVGA-TTRSPEEISPALRSRC----MEIYFRPLLPE--- 270 S+ + +P DF LV A + E++ PALRSR E+Y R +P+ Sbjct: 58 LSSGAM----VRTEPVPCDFILVAAGNLDTIEKMHPALRSRIRGYGYEVYMRTTMPDTPE 113 Query: 271 ---EIAVIGRDAVQKIGLKP--SPEAIEVVQQYATNG-----------REAVNMIQLAAG 314 ++ V+K G P + +A+E + + A R+ +++ A Sbjct: 114 NRRKLVQFVAQEVKKDGRIPHFTRDAVEEIVREAQRRAGRKGHLTLRLRDLGGVVRAAGD 173 Query: 315 LALTEGR------DTLXXXXXXXXXXXXQLPMRTERKIP------SSPQIGLVNGLAVYG 362 +A+ +G+ D L ERK +IG VNGLA+ G Sbjct: 174 IAVRKGKKYVTREDVLEALKLAKPLEKQLADWYIERKKEYQVIRVEGGEIGRVNGLAIIG 233 Query: 363 PGMGTLLEIEVSAAPAKSG-QGRLNVTGVVDE 393 G +L IE APA S +G++ VTG + E Sbjct: 234 EQSGIVLPIEAIVAPAASKEEGKIIVTGKLGE 265 >MA0669 [L] COG0470 ATPase involved in DNA replication Length = 673 Score = 58.2 bits (139), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 17/259 (6%), Positives = 40/259 (15%), Gaps = 64/259 (24%) Query: 61 EKTRPASIGDIVGQKDGLRALKAALCSANPQHVIIYGPPGVGKTAAARVVMEEAKKNGLS 120 A G + + I + + + + Sbjct: 17 PCLVLAGAGSGKTRVITNKIAHLIRGCGYQARHIAAVTFTNKAAREMKERVGQTLGRKEA 76 Query: 121 PFKSDAKFTEIDATTARFDERGIADPLIGSVHDPIYQGAGAMGVAGVPQPKPGAVTKAHG 180 T I + Sbjct: 77 R----GLMISTFHT----LGLD-----IIKREYAALGMKANFSLFDDTDQ---------- 113 Query: 181 GILFLDEIGELHPIQMNKLLKVLEDRKVLLESAYYNSEDSNTPAYIHDIFQNGLPADFRL 240 + L E+ E LL+ L ++++ Sbjct: 114 -LALLKELTEGLIEDDKVLLQQLI----------------------------STISNWKN 144 Query: 241 VGATTRSPEEISPALRSRCMEIYFRPL----LPEEIAVIGRDAVQKIGLKPSPEAIEVVQ 296 T + + + L L + D L P+ Sbjct: 145 DLKTPSQAAASAI-GERDRIFAHCYGLYDAHLKACNVLDFDDL----ILLPTLLLQANE- 198 Query: 297 QYATNG--REAVNMIQLAA 313 + + ++ Sbjct: 199 EVRKRWQNKIRYLLVDEYQ 217 >SMc01045 [T] COG2204 Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains Length = 454 Score = 57.4 bits (137), Expect = 5e-08, Method: Compositional matrix adjust. Identities = 73/277 (26%), Positives = 122/277 (44%), Gaps = 63/277 (22%) Query: 70 DIVGQKDGLRALKAALCSANPQH--VIIYGPPGVGKTAAARVVMEE-AKKNGLSPFKSDA 126 +++G + L+ + P + ++I GP G GK AR++ + A+ NG Sbjct: 141 ELIGTSVAVSQLRQMIEKVAPTNSRIMIQGPSGSGKELVARMIHRKSARANG-------- 192 Query: 127 KFTEIDATTARFDERGIADPLIGSVHDPIYQGAGAMGVAGVPQPKPGAVTKAHGGILFLD 186 F ++A D IA ++ G G G P+ + GA+ +AHGGIL+LD Sbjct: 193 PFVALNAAAITPDRMEIA----------LF---GTEGTTGQPR-RTGALEEAHGGILYLD 238 Query: 187 EIGELHPIQMNKLLKVLEDRKV--------------LLESAYYNSEDSNTPA-YIHDIFQ 231 E+GE+ NK+L+VL D++ ++ S YN E+ T + D++ Sbjct: 239 EVGEMPRETQNKILRVLVDQQFERVGGSKRVKVDVRIISSTAYNLENMITEGLFREDLYH 298 Query: 232 NGLPADFRLVGATTRSPEEISPALRSRCMEIYFRPLLPEEIAVIGRDAVQKIGLKPSP-- 289 RL R PAL R +I P L + + R ++ G++P Sbjct: 299 -------RLAVIPVR-----VPALAERREDI---PFL---VDMFMRQVSEQAGIRPRKIG 340 Query: 290 -EAIEVVQ--QYATNGREAVNMIQLAAGLALTEGRDT 323 +A+ V+Q + N R+ N I+ LA ++G DT Sbjct: 341 EDALAVLQAHDWPGNIRQLRNNIERLMILARSDGPDT 377 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 31,016,894 Number of extensions: 1279560 Number of successful extensions: 4609 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 4637 Number of HSP's successfully gapped: 26 Length of query: 432 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 323 Effective length of database: 25,807,025 Effective search space: 8335669075 Effective search space used: 8335669075 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 117 (49.7 bits)