BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0422.0013.p (233 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC28F2.05c [R] COG0656 Aldo/keto reductases, related to diketo... 219 3e-57 YNL102w [L] COG0417 DNA polymerase elongation subunit (family B) 219 3e-57 CC0005 [L] COG0847 DNA polymerase III, epsilon subunit and relat... 219 3e-57 Cgl1492_1 [I] COG1884 Methylmalonyl-CoA mutase, N-terminal domai... 219 3e-57 PAE2968 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphoryl... 219 3e-57 >SPBC28F2.05c [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 219 bits (557), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 10/219 (4%), Positives = 36/219 (16%) Query: 1 MVLHLDVGREKSVRALEKAMVEDNLILLCSQSEVNIEEPGQEDIFRVGTVANVRQMLKLP 60 + A+ + + ++ + + E + + + Sbjct: 25 EQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLII 84 Query: 61 NGTIRVLVEGVERAEIINYTDNEEYYEVMARVLPEEEDVDQQSDALMRTVLNQFEHYISL 120 + ++ + + + + D + + L Sbjct: 85 ASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKL 144 Query: 121 SKKVTPETLAAVSDIEEPGRLADVITSHLALKIKDKQEILETIDVSKRLEKLLDIXXXXX 180 T A + + + +K Sbjct: 145 GYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTI 204 Query: 181 XXXXXXXKINQRVKKQMEKTQKEYYLREQMKAIQKELGD 219 + V + L L Sbjct: 205 QNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG 243 >YNL102w [L] COG0417 DNA polymerase elongation subunit (family B) Length = 1468 Score = 219 bits (557), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 13/219 (5%), Positives = 48/219 (21%) Query: 1 MVLHLDVGREKSVRALEKAMVEDNLILLCSQSEVNIEEPGQEDIFRVGTVANVRQMLKLP 60 + ++ +++ + +++ G + R Sbjct: 25 DYEGDESDGDRIYDEIDEKEYRARKRQELLHDDFVVDDDGVGYVDRGVEEDWREVDNSSS 84 Query: 61 NGTIRVLVEGVERAEIINYTDNEEYYEVMARVLPEEEDVDQQSDALMRTVLNQFEHYISL 120 + L + + + + M R + + + + + L Sbjct: 85 DEDTGNLASKDSKRKKNIKREKDHQITDMLRTQHSKSTLLAHAKKSQKKSIPIDNFDDIL 144 Query: 121 SKKVTPETLAAVSDIEEPGRLADVITSHLALKIKDKQEILETIDVSKRLEKLLDIXXXXX 180 + + E + R + K K+ Sbjct: 145 GEFESGEVEKPNILLPSKLRENLNSSPTSEFKSSIKRVNGNDESSHDAGISKKVKIDPDS 204 Query: 181 XXXXXXXKINQRVKKQMEKTQKEYYLREQMKAIQKELGD 219 + +K Q K + +++ + ++ Sbjct: 205 STDKYLEIESSPLKLQSRKLRYANDVQDLLDDVENSPVV 243 >CC0005 [L] COG0847 DNA polymerase III, epsilon subunit and related 3'-5' exonucleases Length = 237 Score = 219 bits (557), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 0/219 (0%), Positives = 0/219 (0%) Query: 1 MVLHLDVGREKSVRALEKAMVEDNLILLCSQSEVNIEEPGQEDIFRVGTVANVRQMLKLP 60 + V + R+ + + ++ + + +A+ Sbjct: 25 IGCIEVVDFMPTGRSFHEYCDPLRDMPAEAEKVHGLSSAFLTGKPKFHEIADRFLDFVGD 84 Query: 61 NGTIRVLVEGVERAEIINYTDNEEYYEVMARVLPEEEDVDQQSDALMRTVLNQFEHYISL 120 + + + + R + ++ + ++ + + Sbjct: 85 SVVVAHNAAFDRQFVNFELEKCGKAPIHEERWVDTLAMAKKRFPGMYNSLDALCKRFKIS 144 Query: 121 SKKVTPETLAAVSDIEEPGRLADVITSHLALKIKDKQEILETIDVSKRLEKLLDIXXXXX 180 + + + L AL++ + + + Sbjct: 145 LESRDKHGALIDAHLLAEVYLELQGGKERALELTHSTGAMAVSTAIQGVYAARSRPLAPR 204 Query: 181 XXXXXXXKINQRVKKQMEKTQKEYYLREQMKAIQKELGD 219 Sbjct: 205 243 >Cgl1492_1 [I] COG1884 Methylmalonyl-CoA mutase, N-terminal domain/subunit Length = 346 Score = 219 bits (557), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 10/219 (4%), Positives = 36/219 (16%) Query: 1 MVLHLDVGREKSVRALEKAMVEDNLILLCSQSEVNIEEPGQEDIFRVGTVANVRQMLKLP 60 + A+ + + ++ + + E + + + Sbjct: 25 EQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLII 84 Query: 61 NGTIRVLVEGVERAEIINYTDNEEYYEVMARVLPEEEDVDQQSDALMRTVLNQFEHYISL 120 + ++ + + + + D + + L Sbjct: 85 ASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKL 144 Query: 121 SKKVTPETLAAVSDIEEPGRLADVITSHLALKIKDKQEILETIDVSKRLEKLLDIXXXXX 180 T A + + + +K Sbjct: 145 GYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTI 204 Query: 181 XXXXXXXKINQRVKKQMEKTQKEYYLREQMKAIQKELGD 219 + V + L L Sbjct: 205 QNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTG 243 >PAE2968 [MJ] COG1208 Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon) Length = 211 Score = 219 bits (557), Expect = 3e-57, Method: Compositional matrix adjust. Identities = 0/219 (0%), Positives = 0/219 (0%) Query: 1 MVLHLDVGREKSVRALEKAMVEDNLILLCSQSEVNIEEPGQEDIFRVGTVANVRQMLKLP 60 + + + V N N E L Sbjct: 25 NFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNS 84 Query: 61 NGTIRVLVEGVERAEIINYTDNEEYYEVMARVLPEEEDVDQQSDALMRTVLNQFEHYISL 120 + + + + + + + A + H + Sbjct: 85 KAENIIKTCRILLEQHNGEVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRV 144 Query: 121 SKKVTPETLAAVSDIEEPGRLADVITSHLALKIKDKQEILETIDVSKRLEKLLDIXXXXX 180 + V +EE + T K I Sbjct: 145 CNRTQFAPGKNVEQVEEKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCE 204 Query: 181 XXXXXXXKINQRVKKQMEKTQKEYYLREQMKAIQKELGD 219 Sbjct: 205 243 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.313 0.132 0.346 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,189,605 Number of extensions: 557093 Number of successful extensions: 1637 Number of sequences better than 1.0e-05: 663 Number of HSP's gapped: 1629 Number of HSP's successfully gapped: 663 Length of query: 233 Length of database: 38,378,431 Length adjustment: 103 Effective length of query: 130 Effective length of database: 26,499,029 Effective search space: 3444873770 Effective search space used: 3444873770 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 114 (48.5 bits)