BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0422.0012.p (530 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA1803 [O] COG0466 ATP-dependent Lon protease, bacterial type 647 0.0 DR1974 [O] COG0466 ATP-dependent Lon protease, bacterial type 633 0.0 SPBC28F2.05c [R] COG0656 Aldo/keto reductases, related to diketo... 617 e-176 YNL102w [L] COG0417 DNA polymerase elongation subunit (family B) 617 e-176 CC0005 [L] COG0847 DNA polymerase III, epsilon subunit and relat... 617 e-176 >PA1803 [O] COG0466 ATP-dependent Lon protease, bacterial type Length = 798 Score = 647 bits (1668), Expect = 0.0, Method: Compositional matrix adjust. Identities = 312/517 (60%), Positives = 406/517 (78%), Gaps = 1/517 (0%) Query: 13 KIEKEIDRLEKMPASSAEGGVIRNYVDMLLSLPWTESTEDDLDILKAEQVLDADHYGLEK 72 K E+++L++M SAE V+R+Y+D LL++PW ++ D+ KAE +LDADHYGLE+ Sbjct: 266 KATAELNKLKQMSPMSAEATVVRSYIDWLLNVPWKAESKVRHDLAKAEDILDADHYGLEE 325 Query: 73 PKERVLEYLAVQKLVKKLKGPILCLVGPPGVGKTSLARSIARSLNRKFVRISLGGVRDEA 132 KER+LEYLAVQK VKKLKGP+LCLVGPPGVGKTSLA SIAR+ NRKFVR++LGGVRDEA Sbjct: 326 VKERILEYLAVQKRVKKLKGPVLCLVGPPGVGKTSLAESIARATNRKFVRMALGGVRDEA 385 Query: 133 EIRGHRRTYVGAMPGRIIQGMKTAGSINPVFLLDEIDKMAADFRGDPSAALLEVLDPEQN 192 EIRGHRRTY+G+MPGR+IQ M G NP+FLLDEIDKM +D RGDP++ALLEVLDPEQN Sbjct: 386 EIRGHRRTYIGSMPGRLIQKMTKVGVRNPLFLLDEIDKMGSDMRGDPASALLEVLDPEQN 445 Query: 193 NTFSDHFVELPFDLSNVMFVTTANTVHNIPRPLLDRMEMLFIPGYTELEKLQIASRYLLP 252 + F+DH++E+ +DLS+VMF+ TAN++ NIP PLLDRME++ +PGYTE EK+ IA++YL+P Sbjct: 446 HNFNDHYLEVDYDLSDVMFLCTANSM-NIPAPLLDRMEVIRLPGYTEDEKVNIAAKYLIP 504 Query: 253 KQRKNHGLEEGQLSIEDDTLLKIVREYTRESGVRNLEQQIAALCRKAAKIIVSGEKEQVS 312 KQ + +GL++G+L+ E+ L I+R YTRE+GVR+LE+QIA +CRKA K ++ Q Sbjct: 505 KQVQANGLKKGELTFEEGALRDIIRYYTREAGVRSLERQIAKVCRKAVKEHAKLKRIQAV 564 Query: 313 ILPAEVKDYLGASKYRYGMAELEDQIGTVTGLAWTEVGGDTLLIEVTVVQGTGKLILTGQ 372 + +++YLG K+RYG+AE +DQIG VTGLAWT+VGG+ L IE VV G G+L TG Sbjct: 565 VSSETLENYLGVRKFRYGLAEQQDQIGQVTGLAWTQVGGELLTIEAAVVPGKGQLTKTGS 624 Query: 373 LGDVMKESAQAAFSYTRSKAEELGLAPDFYEKNDIHIHIPEGAIPKDGPSAGITIATALI 432 LGDVM ES AA + RS+A+ LG+A DF+EK DIHIH+PEGA PKDGPSAGI + TAL+ Sbjct: 625 LGDVMAESITAALTVVRSRAQSLGIAADFHEKRDIHIHVPEGATPKDGPSAGIGMCTALV 684 Query: 433 SALTKRYVSKDVAMTGEITLRGRVLPIGGLKEKSLAAHRAGYKKILLPKDNERDLKDIPE 492 SA+T+ V DVAMTGEITLRG+VL IGGLKEK LAAHR G K +++P++N RDLK+IP+ Sbjct: 685 SAITQIPVRADVAMTGEITLRGQVLAIGGLKEKLLAAHRGGIKTVIIPEENVRDLKEIPD 744 Query: 493 SVRSDVEFVPVSHMDQVLKHALVEHHNEISSEAPSSV 529 +++SD+ PV +D+VL+ AL + AP V Sbjct: 745 NIKSDLVIKPVKWIDEVLQIALQYAPEPLPDAAPEMV 781 >DR1974 [O] COG0466 ATP-dependent Lon protease, bacterial type Length = 813 Score = 633 bits (1633), Expect = 0.0, Method: Compositional matrix adjust. Identities = 309/531 (58%), Positives = 399/531 (75%), Gaps = 23/531 (4%) Query: 1 MEEKELPERVKEKIEKEIDRLEKMPASSAEGGVIRNYVDMLLSLPWTESTEDDLDILKAE 60 +EE +PE VK+K KE+ RLE+ P S E V+RNY+D L+ +PW++ E+ LDI + Sbjct: 256 IEEAGMPESVKDKALKELQRLERTPGGSPESTVVRNYIDWLVDVPWSKRDEEILDIGRTR 315 Query: 61 QVLDADHYGLEKPKERVLEYLAVQKLVKK---------------------LKGPILCLVG 99 +LD+DHY L K+R+LE+LAV++L K L+ PILCLVG Sbjct: 316 DILDSDHYALGDVKDRILEFLAVRQLTHKDGETEEQRRERSAEERTDDAELRAPILCLVG 375 Query: 100 PPGVGKTSLARSIARSLNRKFVRISLGGVRDEAEIRGHRRTYVGAMPGRIIQGMKTAGSI 159 PPGVGKTSL +SIARSLNRKFVR++LGGVRDE+EIRGHRRTY+GAMPGRIIQGMKTAG + Sbjct: 376 PPGVGKTSLGKSIARSLNRKFVRMALGGVRDESEIRGHRRTYIGAMPGRIIQGMKTAGVV 435 Query: 160 NPVFLLDEIDKMAADFRGDPSAALLEVLDPEQNNTFSDHFVELPFDLSNVMFVTTANTVH 219 NPV LLDEIDKM++D+RGDPS+A+LEVLDPEQN+TF DH++E+P+DLS VMF+TTAN++ Sbjct: 436 NPVVLLDEIDKMSSDWRGDPSSAMLEVLDPEQNHTFQDHYLEVPYDLSQVMFITTANSLQ 495 Query: 220 NIPRPLLDRMEMLFIPGYTELEKLQIASRYLLPKQRKNHGLEEGQLSIEDDTLLKIVREY 279 IPRPLLDRME++ IPGYT+ EK++IA RY +P+Q K+HGL G+L I D L +IV EY Sbjct: 496 TIPRPLLDRMEVIQIPGYTQQEKVEIAKRYRVPRQIKSHGL-TGKLEITDAALNRIVEEY 554 Query: 280 TRESGVRNLEQQIAALCRKAAKIIVSGEKEQVSILPA-EVKDYLGASKYRYGMAELEDQI 338 T ESGVRNL++QI+ L RKAA+ ++ E + ++ A ++ D+LG +R E E Q+ Sbjct: 555 TAESGVRNLDRQISKLARKAARELLETPWEGLKVIDAPQIPDFLGVPLHRPEKMEKEPQV 614 Query: 339 GTVTGLAWTEVGGDTLLIEVTVVQGTGKLILTGQLGDVMKESAQAAFSYTRSKAEELGLA 398 G GLAWT VGG LL+E GTGK+++TG LGDVMKES AA +Y R+ A E G Sbjct: 615 GVAQGLAWTSVGGTMLLVEALATPGTGKVVMTGSLGDVMKESVGAAIAYLRAHAAEYGAD 674 Query: 399 PDFYEKNDIHIHIPEGAIPKDGPSAGITIATALISALTKRYVSKDVAMTGEITLRGRVLP 458 PDF++ DIH+H P+GA PKDGPSAGITIATA+ISA+T R V DVAMTGEI+LRGRVLP Sbjct: 675 PDFHKTMDIHVHFPDGATPKDGPSAGITIATAVISAVTGRPVRLDVAMTGEISLRGRVLP 734 Query: 459 IGGLKEKSLAAHRAGYKKILLPKDNERDLKDIPESVRSDVEFVPVSHMDQV 509 IGGLKEK LAAH+ G ++++ PKDNE L+++PES+R D++ V H+ +V Sbjct: 735 IGGLKEKLLAAHQGGIREVIFPKDNEPSLQEVPESIRGDLKVHAVEHVSEV 785 >SPBC28F2.05c [R] COG0656 Aldo/keto reductases, related to diketogulonate reductase Length = 346 Score = 617 bits (1592), Expect = e-176, Method: Compositional matrix adjust. Identities = 0/498 (0%), Positives = 0/498 (0%), Gaps = 2/498 (0%) Query: 18 IDRLEKMPASSAEGGVIRNYVDMLLSLPWTESTEDDLDILKAEQVLDADHYGLEKPKERV 77 ++ + + + + E + Sbjct: 275 AAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI 334 Query: 78 LEYLAVQKLVKKLKGPILCLVGPPGVGKTSLARSIARSLNRKFVRISLGGVRDEAEIRGH 137 Sbjct: 335 394 Query: 138 RRTYVGAMPGRIIQGMKTAGSINPVFLLDEIDKMAADFRGDPSAALLEVLDPEQNNTFSD 197 Sbjct: 395 454 Query: 198 HFVELPFDLSNVMFVTTANTVHNIPRPLLDRMEMLFIPGYTELEKLQIASRYLLPKQRKN 257 Sbjct: 455 - 513 Query: 258 HGLEEGQLSIEDDTLLKIVREYTRESGVRNLEQQIAALCRKAAK-IIVSGEKEQVSILPA 316 Sbjct: 514 573 Query: 317 EVKDYLGASKYRYGMAELEDQIGTVTGLAWTEVGGDTLLIEVTVVQGTGKLILTGQLGDV 376 Sbjct: 574 633 Query: 377 MKESAQAAFSYTRSKAEELGLAPDFYEKNDIHIHIPEGAIPKDGPSAGITIATALISALT 436 Sbjct: 634 693 Query: 437 KRYVSKDVAMTGEITLRGRVLPIGGLKEKSLAAHRAGYKKILLPKDNERDLKDIPESVRS 496 Sbjct: 694 753 Query: 497 DVEFVPVSHMDQVLKHAL 514 Sbjct: 754 771 >YNL102w [L] COG0417 DNA polymerase elongation subunit (family B) Length = 1468 Score = 617 bits (1592), Expect = e-176, Method: Compositional matrix adjust. Identities = 30/498 (6%), Positives = 93/498 (18%), Gaps = 2/498 (0%) Query: 18 IDRLEKMPASSAEGGVIRNYVDMLLSLPWTESTEDDLDILKAEQVLDADHYGLEKPKERV 77 + + + R S P T + L Sbjct: 275 DEDIILKRRTMRSVTTTRRVNIDSRSNPSTSPFVTAPGTPIGIKGLTPSKSLQSNTDVAT 334 Query: 78 LEYLAVQKLVKKLKGPILCLVGPPGVGKTSLARSIARSLNRKFVRISLGGVRDEAEIRGH 137 L ++ V + + + + + +S + Sbjct: 335 LAVNVKKEDVVDPETDTFQMFWLDYCEVNNTLILFGKVKLKDDNCVSAMVQINGLCRELF 394 Query: 138 RRTYVGAMPGRIIQGMKTAGSINPVFLLDEIDKMAADFRGDPSAALLEVLDPEQNNTFSD 197 G P I + + + + E + + Sbjct: 395 FLPREGKTPTDIHEEIIPLLMDKYGLDNIRAKPQKMKYSFELPDIPSESDYLKVLLPYQT 454 Query: 198 HFVELPFDLSNVMFVTTANTVHNIPRPLLDRMEMLFIPGYTELEKLQIASRYLLPKQRKN 257 S++ T + + + + ++ A + Sbjct: 455 PKSSRDTIPSDLSSDTFYHVF-GGNSNIFESFVIQNRIMGPCWLDIKGADFNSIRNASHC 513 Query: 258 HGLEEGQLSIEDDTLLKIVREYTRESGVRNLEQQIAALCRKAAK-IIVSGEKEQVSILPA 316 R + ++ I +S + P Sbjct: 514 AVEVSVDKPQNITPTTTKTMPNLRCLSLSIQTLMNPKENKQEIVSITLSAYRNISLDSPI 573 Query: 317 EVKDYLGASKYRYGMAELEDQIGTVTGLAWTEVGGDTLLIEVTVVQGTGKLILTGQLGDV 376 + + LA ++ G L + + Sbjct: 574 PENIKPDDLCTLVRPPQSTSFPLGLAALAKQKLPGRVRLFNNEKAMLSCFCAMLKVEDPD 633 Query: 377 MKESAQAAFSYTRSKAEELGLAPDFYEKNDIHIHIPEGAIPKDGPSAGITIATALISALT 436 + + Y A + + ++ + Sbjct: 634 VIIGHRLQNVYLDVLAHRMHDLNIPTFSSIGRRLRRTWPEKFGRGNSNMNHFFISDICSG 693 Query: 437 KRYVSKDVAMTGEITLRGRVLPIGGLKEKSLAAHRAGYKKILLPKDNERDLKDIPESVRS 496 + M +T + + + + + + + D+ + +++ Sbjct: 694 RLICDIANEMGQSLTPKCQSWDLSEMYQVTCEKEHKPLDIDYQNPQYQNDVNSMTMALQE 753 Query: 497 DVEFVPVSHMDQVLKHAL 514 ++ +S L Sbjct: 754 NITNCMISAEVSYRIQLL 771 >CC0005 [L] COG0847 DNA polymerase III, epsilon subunit and related 3'-5' exonucleases Length = 237 Score = 617 bits (1592), Expect = e-176, Method: Compositional matrix adjust. Identities = 0/498 (0%), Positives = 0/498 (0%), Gaps = 2/498 (0%) Query: 18 IDRLEKMPASSAEGGVIRNYVDMLLSLPWTESTEDDLDILKAEQVLDADHYGLEKPKERV 77 Sbjct: 275 334 Query: 78 LEYLAVQKLVKKLKGPILCLVGPPGVGKTSLARSIARSLNRKFVRISLGGVRDEAEIRGH 137 Sbjct: 335 394 Query: 138 RRTYVGAMPGRIIQGMKTAGSINPVFLLDEIDKMAADFRGDPSAALLEVLDPEQNNTFSD 197 Sbjct: 395 454 Query: 198 HFVELPFDLSNVMFVTTANTVHNIPRPLLDRMEMLFIPGYTELEKLQIASRYLLPKQRKN 257 Sbjct: 455 - 513 Query: 258 HGLEEGQLSIEDDTLLKIVREYTRESGVRNLEQQIAALCRKAAK-IIVSGEKEQVSILPA 316 Sbjct: 514 573 Query: 317 EVKDYLGASKYRYGMAELEDQIGTVTGLAWTEVGGDTLLIEVTVVQGTGKLILTGQLGDV 376 Sbjct: 574 633 Query: 377 MKESAQAAFSYTRSKAEELGLAPDFYEKNDIHIHIPEGAIPKDGPSAGITIATALISALT 436 Sbjct: 634 693 Query: 437 KRYVSKDVAMTGEITLRGRVLPIGGLKEKSLAAHRAGYKKILLPKDNERDLKDIPESVRS 496 Sbjct: 694 753 Query: 497 DVEFVPVSHMDQVLKHAL 514 Sbjct: 754 771 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.135 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 39,696,369 Number of extensions: 1713213 Number of successful extensions: 18653 Number of sequences better than 1.0e-05: 680 Number of HSP's gapped: 17349 Number of HSP's successfully gapped: 681 Length of query: 530 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 419 Effective length of database: 25,576,357 Effective search space: 10716493583 Effective search space used: 10716493583 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 118 (50.1 bits)