BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0422.0007.p (270 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ywqC [M] COG3944 Capsular polysaccharide biosynthesis protein 141 1e-33 BS_yveK [M] COG3944 Capsular polysaccharide biosynthesis protein 107 2e-23 SA2457 [M] COG3944 Capsular polysaccharide biosynthesis protein 106 4e-23 CAC3044 [M] COG3944 Capsular polysaccharide biosynthesis protein 102 7e-22 SA0144 [M] COG3944 Capsular polysaccharide biosynthesis protein 92 1e-18 >BS_ywqC [M] COG3944 Capsular polysaccharide biosynthesis protein Length = 248 Score = 141 bits (356), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 84/239 (35%), Positives = 136/239 (56%), Gaps = 5/239 (2%) Query: 15 AQELDLRDYFQIVRKRLWMIVSIVVAVCILAGVYSLYIKNPVYEASTKIIVNQTPTQSTV 74 + L++ + KR+ +I+ + A G+ S + P+YE ST+I+VNQ+ + Sbjct: 2 GESTSLKEILSTLTKRILLIMIVTAAATAAGGLISFFALTPIYENSTQILVNQSKNER-- 59 Query: 75 AXXXXXXXXXXXXXXXXYKEIIKTPAILDVVAKNYPQFNLTAEDLLKKVNVSSVNNTQVM 134 Y IIK+PAILD V K ++T+++L K+ VSS ++QV+ Sbjct: 60 KEVQFNDVQTNLQLINTYNVIIKSPAILDEVIKEMG-LSMTSQELNDKITVSSEQDSQVV 118 Query: 135 TLVVRDNSYQRAAEIVNAISLVFKQEIPSLFNVQNVSILNEAKVDPPVAPGPVEPNVVMN 194 + VRD + + AA I N I+ VF+ +I S+ NV NVSIL++A+V P PV P ++N Sbjct: 119 NISVRDENAETAAHIANTIASVFQDKITSIMNVDNVSILSKAEVSE--HPSPVSPKPLLN 176 Query: 195 LAIAFIVSLMIGLGIAFLLEYLDDTLKTEEDIEKYLGLPTIAMITRLGQEETKSTELQA 253 +AIAF L +G+AFLLE+LD+T+K+EE +E L +P + ++ + E+ + LQ Sbjct: 177 IAIAFAAGLAGSIGLAFLLEHLDNTIKSEEQLESLLDIPVLGTVSTIANEQKTAKTLQG 235 >BS_yveK [M] COG3944 Capsular polysaccharide biosynthesis protein Length = 234 Score = 107 bits (267), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 62/224 (27%), Positives = 121/224 (54%), Gaps = 9/224 (4%) Query: 16 QELDLRDYFQIVRKRLWMIVSIVVAVCILAGVYSLYIKNPVYEASTKIIVNQTPTQSTVA 75 + + ++ + IVR R +I+ I + V ++ G + +P Y+AST+++V+++ + Sbjct: 3 ENMSFKELYAIVRHRFVLILLITIGVTLIMGFVQFKVISPTYQASTQVLVHESDGEEN-- 60 Query: 76 XXXXXXXXXXXXXXXXYKEIIKTPAILDVVAKNYPQFNLTAEDLLKKVNVSSVNNTQVMT 135 ++ I+K+ A+++ V K + +A L KV SS N ++++ Sbjct: 61 -SNLSDIQRNLQYSSTFQSIMKSTALMEEV-KAELHLSESASSLKGKVVTSSENESEIIN 118 Query: 136 LVVRDNSYQRAAEIVNAISLVFKQEIPSLFNVQNVSILNEAKV-DPPVAPGPVEPNVVMN 194 + V+D+ +AAEI N + F++E+ NVQ V IL+EAK + P+ ++P + N Sbjct: 119 VAVQDHDPAKAAEIANTLVNKFEKEVDERMNVQGVHILSEAKASESPM----IKPARLRN 174 Query: 195 LAIAFIVSLMIGLGIAFLLEYLDDTLKTEEDIEKYLGLPTIAMI 238 + +AF ++M G+ +AF L +LDDT K+ + + GLP + + Sbjct: 175 MVMAFGAAVMGGITLAFFLHFLDDTCKSARQLSERTGLPCLGSV 218 >SA2457 [M] COG3944 Capsular polysaccharide biosynthesis protein Length = 220 Score = 106 bits (264), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 75/227 (33%), Positives = 126/227 (55%), Gaps = 10/227 (4%) Query: 16 QELDLRDYFQIVRKRLWMIVSIVVAVC-ILAGVYSLYIKNPVYEASTKIIVNQTPTQSTV 74 ++ DL I++K + +++ I+ A+C +++ + ++ Y AST+I+VN + S + Sbjct: 3 EKFDLVKLLNILKKNIKLLL-ILPAICLVVSTALTFFVMPDKYTASTQILVNMKKSSSDL 61 Query: 75 AXXXXXXXXXXXXXXXXYKEIIKTPAILDVVAKNYPQFNLTAEDLLKKVNVSSVNNTQVM 134 A Y EIIK+P ILD V++ + TAE L + V++ N+Q++ Sbjct: 62 AFQNVQSSLQSVNT---YTEIIKSPRILDKVSREFDGQYSTAE-LNSFLKVTNQTNSQII 117 Query: 135 TLVVRDNSYQRAAEIVNAISLVFKQEIPSLFNVQNVSILNEAKVDPPVAPGPVEPNVVMN 194 T+ V + + +IVN IS VF ++P + +V NV+IL+ A D V P+ V +N Sbjct: 118 TVSVTTGNKSESDKIVNRISKVFAHDMPKIMSVDNVTILSSAH-DNAVKVSPI---VSVN 173 Query: 195 LAIAFIVSLMIGLGIAFLLEYLDDTLKTEEDIEKYLGLPTIAMITRL 241 L I+ IV +++ + I FL E LD +KTEED+E LGLP + I + Sbjct: 174 LVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQKF 220 >CAC3044 [M] COG3944 Capsular polysaccharide biosynthesis protein Length = 211 Score = 102 bits (253), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 0/229 (0%), Positives = 0/229 (0%), Gaps = 8/229 (3%) Query: 17 ELDLRDYFQIVRKRLWMIVSIVVAVCILAGVYSLYIKNPVYEASTKIIVNQTPTQSTVAX 76 L++ + ++ +L V V + Sbjct: 6 RLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATDVSVNKATAKLYPVANTPAA-M 64 Query: 77 XXXXXXXXXXXXXXXYKEIIKTPAILDVVAKNYPQFNLTAEDLLKKVNVSSVNNTQVMTL 136 K I+ Q + + + + Sbjct: 65 LELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQ-HNGEVPEDRAALEALPGVGRKTAN 123 Query: 137 VVRDNSYQRAAEIVNAISLVFKQEIPSLFNVQNVSILNEAKVDPPVAP-GPVEPNVVMNL 195 VV + ++ V+ + + + + Sbjct: 124 VVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVEEKL-----LKVVPAEFKVDCHHW 178 Query: 196 AIAFIVSLMIGLGIAFLLEYLDDTLKTEEDIEKYLGLPTIAMITRLGQE 244 Sbjct: 179 227 >SA0144 [M] COG3944 Capsular polysaccharide biosynthesis protein Length = 222 Score = 91.7 bits (226), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 61/197 (30%), Positives = 102/197 (51%), Gaps = 7/197 (3%) Query: 45 AGVYSLYIKNPVYEASTKIIVNQTPTQSTVAXXXXXXXXXXXXXXXXYKEIIKTPAILDV 104 + + + ++ +P Y+A+T+I+VNQT + YKEI+K+P ILD Sbjct: 32 SAIVTFFVLSPKYQANTQILVNQTKGDN--PQFMAQEVQSNIQLVNTYKEIVKSPRILDE 89 Query: 105 VAKNYPQFNLTAEDLLKKVNVSSVNNTQVMTLVVRDNSYQRAAEIVNAISLVFKQEIPSL 164 V+K+ + L + +++ NTQ++ + V+ Q + +I N+ + V ++IP + Sbjct: 90 VSKDLND-KYSPSKLSSMLTITNQENTQLINIQVKSGHKQDSEKIANSFAKVTSKQIPKI 148 Query: 165 FNVQNVSILNEAKVDPPVAPGPVEPNVVMNLAIAFIVSLMIGLGIAFLLEYLDDTLKTEE 224 +V NVSIL +K D V P V+NL AF + L++ L F D +K EE Sbjct: 149 MSVDNVSIL--SKADGTAVK--VAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEE 204 Query: 225 DIEKYLGLPTIAMITRL 241 D+EK LGLP + I + Sbjct: 205 DVEKELGLPVLGSIQKF 221 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.133 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,730,130 Number of extensions: 611826 Number of successful extensions: 1419 Number of sequences better than 1.0e-05: 6 Number of HSP's gapped: 1408 Number of HSP's successfully gapped: 6 Length of query: 270 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 165 Effective length of database: 26,268,361 Effective search space: 4334279565 Effective search space used: 4334279565 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)