BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0037.p (565 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2927 [G] COG0366 Glycosidases 349 7e-96 lin2231 [G] COG0366 Glycosidases 342 9e-94 BS_yvdF [G] COG0366 Glycosidases 337 2e-92 SPy1304 [G] COG0366 Glycosidases 329 8e-90 L128694 [G] COG0366 Glycosidases 327 3e-89 >BH2927 [G] COG0366 Glycosidases Length = 578 Score = 349 bits (896), Expect = 7e-96, Method: Compositional matrix adjust. Identities = 207/573 (36%), Positives = 305/573 (53%), Gaps = 48/573 (8%) Query: 6 LYHTAEDPFAFPVGSGLLKLRLFVQNGQQLSCTVIHSDRYDTPGSEIPLPMERIGSAGAY 65 +YH + A+ + +RL +I+ D YD ++ + + G+ Sbjct: 5 IYHRPKSEDAYAYDKSTMHIRLRTGKNHVRKVNLIYGDPYDWTKNQWVTSKKEMTPTGSD 64 Query: 66 EIHEAVLQSATG---KCRYLFHVENPAGDYVWYGERGL--SENREHAGSFQYAYMHRPEA 120 H+ T + RY F + + ++YGE G + +++ + F + +M+ + Sbjct: 65 AQHDFWFVEVTPPYRRLRYGFEIITKT-ETIYYGESGFLDTPSKDTSDYFCFPFMNPADL 123 Query: 121 VKLPSWSSDAVVYQIFPNSYN-------------------------GGTLKGITEKIPYL 155 P W D V YQIFP + GG L+GI + + YL Sbjct: 124 FSPPEWVKDTVWYQIFPERFANGDPSRNPEGTLPWGSAAPTPTNHFGGDLQGIIDHLDYL 183 Query: 156 HQLGITALYMTPIFDSPSAHKYNTSDYYKVDPAFGSTEDLKALVDEAHRHGIRVLLDAVF 215 +LGI +Y+TPIF++ S HKY+T DY KVDP FG K LVDE H+ GIR++LDAVF Sbjct: 184 QELGINGIYLTPIFEASSNHKYDTIDYLKVDPHFGDKTTFKRLVDECHKRGIRIMLDAVF 243 Query: 216 NHSGDAFFAFKDVLEHGENSRYKDWFVIHSFPVVQTPSPNYETFAKAEAHMPKLNMDNPD 275 NH+G F F+DVL++ ++S+Y DWF + FPV PNY+TFA MPK N +N + Sbjct: 244 NHAGFYFPPFQDVLKNQQHSKYSDWFHLRDFPVRAEEPPNYDTFA-FTPFMPKFNTENEE 302 Query: 276 VADYMMEVSKHWVREAGIDGWRLDVANEVSPRFWTRFRRELKAEFPELLLIGEIMHDSGP 335 V +Y++ V+ +W++E IDGWRLDVANEV FW FR+ +KA P+L ++GE+ H+S P Sbjct: 303 VREYLLNVATYWIKEFDIDGWRLDVANEVDHAFWREFRKRVKALKPDLYILGEVWHNSLP 362 Query: 336 WLRGDQFDGGMNYVLRDAVLEFFAEQYAGPVRFMEQLLHQEALCNDQANSAMFQLLGSHD 395 WL GDQFD MNY L +A+++F +Q G RF + + F LLGSHD Sbjct: 363 WLAGDQFDAVMNYPLTNAIIDFAGKQKIGQQRFEHVITQLTHMYPIHVQQVAFNLLGSHD 422 Query: 396 TLRFLTACKQGGRGWDHENTAVQRMRLAVFFQMTYIGMPMIYYGDEVGMEGATDPHCRLP 455 T R LT + V R++L + G P IYYGDE+GM G DP CR Sbjct: 423 TPRILTQT----------DNDVARVKLQFLLLFSQRGTPCIYYGDEIGMTGGQDPGCRKC 472 Query: 456 MVWKEQEQNTALHAWYTQLISLRKQYEVLRKGAFRPWITDEARNVLGYIRCSGQDQMGLL 515 MVW+E+EQN L ++ +LI LRKQ + ++ +++ ++L Y + Q+ + Sbjct: 473 MVWEEEEQNQELLSFVKRLIQLRKQEKAFGTEGDLTFVENDS-SLLIYQKTYENQQLLFV 531 Query: 516 INNS--PNRYQLELSRFRSDANVLTDLLTGATF 546 IN++ P+ QL +S +AN DLLTG TF Sbjct: 532 INSTDQPDSVQLPVSWTNRNAN---DLLTGETF 561 >lin2231 [G] COG0366 Glycosidases Length = 591 Score = 342 bits (877), Expect = 9e-94, Method: Compositional matrix adjust. Identities = 185/529 (34%), Positives = 277/529 (52%), Gaps = 52/529 (9%) Query: 6 LYHTAEDPFAFPVGSGLLKLRLFVQNGQQLSCTVIHSDRY---DTPGSEIPLPMERIGSA 62 +YH +A+ + L +R+ + T+I +D Y + M +I Sbjct: 6 IYHQPASSYAYSYDAKTLHIRIRTKRLDISKVTLIAADPYLWKNEKWQSESYSMRKIAET 65 Query: 63 GAYEIHEAVLQSATGKCRYLFHVENPAGDYVWYGERGLSENREHAGS-----FQYAYMHR 117 ++ + + +Y F + + G+ +YG RG E E + F++ ++H Sbjct: 66 EEHDYWFFAITPEHRRLQYGFLLTDTEGETTFYGGRGFFEPTEANLATMDYYFKFPFIHA 125 Query: 118 PEAVKLPSWSSDAVVYQIFPNSYN-------------------------GGTLKGITEKI 152 + + P W + + YQIFP + GG L+GI + + Sbjct: 126 VDTFEAPEWVGNTIWYQIFPERFANGNPAISPENALPWGSKDPNTTDFFGGDLEGIIQHL 185 Query: 153 PYLHQLGITALYMTPIFDSPSAHKYNTSDYYKVDPAFGSTEDLKALVDEAHRHGIRVLLD 212 YL +LGI +Y+TP+F++P+ HKY+T DY K+DP FG E + LV EAH+ GIR++LD Sbjct: 186 DYLAELGINGVYLTPVFEAPTNHKYDTIDYKKIDPHFGDKETFRKLVQEAHKRGIRIMLD 245 Query: 213 AVFNHSGDAFFAFKDVLEHGENSRYKDWFVIHSFPVVQT--------PSPNYETFAKAEA 264 AVFNH GD ++DV+E E S Y+DWF IHSFPV Q P+ +Y+TFA Sbjct: 246 AVFNHIGDTSLEWQDVVEKEEKSAYRDWFHIHSFPVRQNENGNIEGEPTLSYDTFAFT-T 304 Query: 265 HMPKLNMDNPDVADYMMEVSKHWVREAGIDGWRLDVANEVSPRFWTRFRRELKAEFPELL 324 HMPKLN NP+V Y+++++ +W+RE IDGWRLDVANEV FW F++ ++AE ++ Sbjct: 305 HMPKLNTANPEVQKYLLDIATYWIREFDIDGWRLDVANEVDHAFWKEFKKAVQAEKEDIY 364 Query: 325 LIGEIMHDSGPWLRGDQFDGGMNYVLRDAVLEFFAEQYAGPVRFMEQLLHQEALCNDQAN 384 ++GEI HDS WL GD+F MNY ++E F E+ P + + + Q +Q N Sbjct: 365 ILGEIWHDSWIWLLGDEFHAVMNYPFTQTIIENFIEEKITPEQMVSGINEQYMRYPNQVN 424 Query: 385 SAMFQLLGSHDTLRFLTACKQGGRGWDHENTAVQRMRLAVFFQMTYIGMPMIYYGDEVGM 444 MF +L SHDT R LT N +++ A+ F + G P IYYG E+GM Sbjct: 425 EVMFNMLDSHDTARILTRA----------NNDEDKVKQALAFMFAHTGSPCIYYGTEIGM 474 Query: 445 EGATDPHCRLPMVWKEQEQNTALHAWYTQLISLRKQYEVLRKGAFRPWI 493 +G DP CR M W E +QN + A+ QLI+LRK + + W+ Sbjct: 475 DGGNDPGCRKCMEWDETKQNQDMLAFTKQLIALRKDNQAIITSGELNWL 523 >BS_yvdF [G] COG0366 Glycosidases Length = 589 Score = 337 bits (865), Expect = 2e-92, Method: Compositional matrix adjust. Identities = 194/564 (34%), Positives = 287/564 (50%), Gaps = 49/564 (8%) Query: 6 LYHTAEDPFAFPVGSGLLKLRLFVQNGQQLSCTVIHSDRY---DTPGSEIPLPMERIGSA 62 ++H A+ + +++ + G I D Y D S PM +I + Sbjct: 7 IHHQPFSTDAYSYDGRTVHIKIRTKKGDADHIRFIWGDPYEYNDGKWSANEQPMRKIAAT 66 Query: 63 GAYEIHEAVLQSATGKCRYLFHVENPAGDYVWYGERGLSENREHAGS-----FQYAYMHR 117 ++ A + + +Y F V + D +++G G+ E F++ ++H Sbjct: 67 EMHDYWFAEVVPPFRRLQYAFVVTDDHED-IFFGSSGVCPYNEKTLETIHYYFKFPFVHE 125 Query: 118 PEAVKLPSWSSDAVVYQIFP-------------------------NSYNGGTLKGITEKI 152 + + P W V YQIFP N + GG L+GI +K+ Sbjct: 126 ADTFQAPEWVKSTVWYQIFPERFANGREDLSPKNALPWGSKDPDVNDFFGGDLQGIVDKL 185 Query: 153 PYLHQLGITALYMTPIFDSPSAHKYNTSDYYKVDPAFGSTEDLKALVDEAHRHGIRVLLD 212 YL LG+ +Y+TPIF +PS HKY+T DY+ +DP FG E + LV + H+ G+R++LD Sbjct: 186 DYLEDLGVNGIYLTPIFSAPSNHKYDTLDYFSIDPHFGDPELFRTLVSQLHQRGMRIMLD 245 Query: 213 AVFNHSGDAFFAFKDVLEHGENSRYKDWFVIHSFPVVQTPSPNYETFAKAEAHMPKLNMD 272 AVFNH G A ++DV+++G+ SRYKDWF IHSFPV NY+ FA A MPKLN Sbjct: 246 AVFNHIGSASPQWQDVVKNGDQSRYKDWFHIHSFPVTDD---NYDRFAFT-ADMPKLNTA 301 Query: 273 NPDVADYMMEVSKHWVREAGIDGWRLDVANEVSPRFWTRFRRELKAEFPELLLIGEIMHD 332 NP+V Y+++++ +W+RE IDGWRLDVANEV FW FR+ + E P++ ++GEI H Sbjct: 302 NPEVQKYLLDIALYWIREFDIDGWRLDVANEVDHVFWKTFRQAVSTEKPDVYILGEIWHS 361 Query: 333 SGPWLRGDQFDGGMNYVLRDAVLEFFAEQYAGPVRFMEQLLHQEALCNDQANSAMFQLLG 392 + PWLRGD+F MNY + ++E+FA+Q R ++ QAN MF LL Sbjct: 362 AEPWLRGDEFHAAMNYPFTEPMIEYFADQTISASRMAHRVNAHLMNGMKQANEVMFNLLD 421 Query: 393 SHDTLRFLTACKQGGRGWDHENTAVQRMRLAVFFQMTYIGMPMIYYGDEVGMEGATDPHC 452 SHDT R LT C+ + R LA F T G P IYYG E+G+ G DP C Sbjct: 422 SHDTKRLLTRCRNDEKK--------ARALLAFMFAQT--GSPCIYYGTEIGLNGENDPLC 471 Query: 453 RLPMVWKEQEQNTALHAWYTQLISLRKQYEVLRKGAFRPW-ITDEARNVLGYIRCSGQDQ 511 R MVW++++QN + + +LI+LRKQ L W + D+ + + + R + Sbjct: 472 RKCMVWEKEKQNQDMLQFMKRLIALRKQENTLLTEGHLEWNLLDDKNDFISFSRTLDEKI 531 Query: 512 MGLLINNSPNRYQLELSRFRSDAN 535 + N + L D N Sbjct: 532 LIYFFNQGNVVQHVSLRELNIDRN 555 >SPy1304 [G] COG0366 Glycosidases Length = 567 Score = 329 bits (843), Expect = 8e-90, Method: Compositional matrix adjust. Identities = 194/554 (35%), Positives = 294/554 (53%), Gaps = 53/554 (9%) Query: 6 LYHTAEDPFAFPVGSGLLKLRLFV-QNGQQLSCTVIHSDRYDTPGSEIPLPMERIGSAGA 64 + H + + F + L +RL V + + ++ ++H +Y + L M + Sbjct: 6 MLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGPKYGYHEFQEELEMSVAYTDHT 65 Query: 65 YEIHEAVLQSATGKCRYLFHVENPAGDYVWYGERGLSENREHAGSF----QYAYMHRPEA 120 + +E L + Y+F +E Y ++ E GL+ + + + F Q Y++ + Sbjct: 66 HAYYETTLTLDDVRLAYIFKIEEEGQTY-YFSEDGLTTSYDFSNGFYNFFQMPYINAVDV 124 Query: 121 VKLPSWSSDAVVYQIF-------------------------PNSYNGGTLKGITEKIPYL 155 ++ W+ DAV YQIF P S+ GG LKGITEK+ YL Sbjct: 125 HQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGWLEKPTPKSFAGGDLKGITEKLDYL 184 Query: 156 HQLGITALYMTPIFDSPSAHKYNTSDYYKVDPAFGSTEDLKALVDEAHRHGIRVLLDAVF 215 LGIT +Y+TPIF S S HKY+ SDYY +DP FG+ DL+ L+D AH+ GI+++LDAVF Sbjct: 185 KDLGITVIYLTPIFQSISNHKYDISDYYAIDPQFGTKYDLQELIDLAHKMGIKIILDAVF 244 Query: 216 NHSGDAFFAFKDVLEHGENSRYKDWFVIHSFPVVQTPS---PNYETFAKAEAHMPKLNMD 272 NH+ F+DVL +G+ S++ DWF+ H + PS NYETFA +MPK N Sbjct: 245 NHASSDAVEFQDVLRYGKESKFFDWFMTHD----EHPSMDLVNYETFAGCN-YMPKWNTS 299 Query: 273 NPDVADYMMEVSKHWVREAGIDGWRLDVANEVSPRFWTRFRRELKAEFPELLLIGEIMHD 332 N DV DY++E+ ++W++E IDGWRLDV++EVS FW RFR+ +KAE + +LIGE HD Sbjct: 300 NRDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSHDFWRRFRQAVKAEKADAILIGENWHD 359 Query: 333 SGPWLRGDQFDGGMNYVLRDAVLEFFAEQYAGPVRFMEQLLHQEALCNDQANSAMFQLLG 392 + P+L GDQ+DG MNY A L++FA + + E+L + Q N LL Sbjct: 360 AYPYLAGDQYDGIMNYAFTKACLDYFAFETMTSKQLAEKLSNILMRNTWQVNRMNLNLLD 419 Query: 393 SHDTLRFLTACKQGGRGWDHENTAVQRMRLAVFFQMTYIGMPMIYYGDEVGMEGATDPHC 452 SHDT RF T K + ++ A+ ++G+P +YYG EV MEG DP Sbjct: 420 SHDTHRFFTQVK----------GSKDKLLAALALLFIFMGIPCLYYGTEVAMEGGYDPDS 469 Query: 453 RLPMVWKEQEQNTALHAWYTQLISLRKQYEVLRKGAFRPWITDEARNVLGYIRCSGQDQM 512 R W + E++ + +I+LR+Q L++GA D + L R G+ + Sbjct: 470 RRGFPWDDAERDKDFFSKVKAIIALRQQ-PALQEGAIS---IDYDEDCLILKRSLGEQSI 525 Query: 513 GLLINNSPNRYQLE 526 L++NN + Y ++ Sbjct: 526 SLVLNNGESAYSIK 539 >L128694 [G] COG0366 Glycosidases Length = 584 Score = 327 bits (838), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 184/550 (33%), Positives = 273/550 (49%), Gaps = 58/550 (10%) Query: 6 LYHTAEDPFAFPVGSGLLKLRLFVQNGQQLSCTVIHSDRYDTPGSEIP---------LPM 56 +YH E FA+ L+ +RL Q + + D Y G + P + M Sbjct: 6 IYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGLDNPETQYWASQKVEM 65 Query: 57 ERIGSAGAYEIHEAVLQSATGKCRYLFHVENPAGDYVWYGERGLSENREHA-----GSFQ 111 ++ S + EA + AT + YLF + G+ V Y ++G+ + E G+F+ Sbjct: 66 KKYLSTNISDYFEAEITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDESLVTKKYGAFR 125 Query: 112 YAYMHRPEAVKLPSWSSDAVVYQIFP--------------------------NSYNGGTL 145 Y H + K P W + V YQIFP + GG L Sbjct: 126 LPYFHESDRFKAPEWVKETVWYQIFPERFANGDTSNDPEGTKAWNPQDVPDRQDFYGGDL 185 Query: 146 KGITEKIPYLHQLGITALYMTPIFDSPSAHKYNTSDYYKVDPAFGSTEDLKALVDEAHRH 205 +G+ + + +L +LG+ +Y TPIF + S HKY+T DY ++D FG E K LV EAH Sbjct: 186 QGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLVKEAHAR 245 Query: 206 GIRVLLDAVFNHSGDAFFAFKDVLEHGENSRYKDWFVIHSFPVVQTPSPNYETFAKA--- 262 GI+V+LDAVFNH GD ++DVL++ E S++ DWF ++S+P TP+ ++E A Sbjct: 246 GIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENAENATYD 305 Query: 263 ----EAHMPKLNMDNPDVADYMMEVSKHWVREAGIDGWRLDVANEVSPRFWTRFRRELKA 318 HMPKLN NP+V Y+++V+ +W+ E ID WRLDVA+EV FW +FR A Sbjct: 306 TFAFTPHMPKLNTANPEVEAYLLKVADYWISEFDIDAWRLDVADEVDHTFWKKFRTTCDA 365 Query: 319 EFPELLLIGEIMHDSGPWLRGDQFDGGMNYVLRDAVLEFFAEQYAGPVRFMEQLLHQEAL 378 + ++GE+ H + PWL GD+F MNY DA+ + + + + + Q L Sbjct: 366 AKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDAIKDGLITKKISLEQMVSNINTQLTL 425 Query: 379 CNDQANSAMFQLLGSHDTLRFLTACKQGGRGWDHENTAVQRMRLAVFFQMTYIGMPMIYY 438 Q N MF +L SHDT R LT K M+L F G+P IYY Sbjct: 426 YRKQVNQMMFNVLDSHDTPRILTVAKNNKN----------LMKLVETFTFMQPGVPCIYY 475 Query: 439 GDEVGMEGATDPHCRLPMVWKEQEQNTALHAWYTQLISLRKQYEVLRKGAFRPW-ITDEA 497 GDE + G DP CR M W+E++Q+ + ++ LI+LRKQY+ + + W I D Sbjct: 476 GDEYAVTGGMDPECRKVMPWEEKDQDLEMFDFFKNLIALRKQYQGILSDSEVEWKIVDSE 535 Query: 498 RNVLGYIRCS 507 ++ + R S Sbjct: 536 NGIVKFERGS 545 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.138 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 45,157,696 Number of extensions: 1928934 Number of successful extensions: 6171 Number of sequences better than 1.0e-05: 118 Number of HSP's gapped: 5899 Number of HSP's successfully gapped: 129 Length of query: 565 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 453 Effective length of database: 25,461,023 Effective search space: 11533843419 Effective search space used: 11533843419 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)