BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0031.p (355 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yunA [M] COG0739 Membrane proteins related to metalloendopept... 329 5e-90 BH3436 [M] COG0739 Membrane proteins related to metalloendopepti... 321 1e-87 DR0598 [M] COG0739 Membrane proteins related to metalloendopepti... 80 4e-15 RC0557 [M] COG0739 Membrane proteins related to metalloendopepti... 59 1e-08 BMEI0197 [M] COG0739 Membrane proteins related to metalloendopep... 58 2e-08 >BS_yunA [M] COG0739 Membrane proteins related to metalloendopeptidases Length = 349 Score = 329 bits (843), Expect = 5e-90, Method: Compositional matrix adjust. Identities = 154/311 (49%), Positives = 202/311 (64%), Gaps = 5/311 (1%) Query: 45 AASGK---ESLSVSRKQLYDQMSAATGIAWFRFAAIDQYERTIAKKKSGTAGQAQPRLTG 101 +ASGK + + R +LY ++ A T I W+ AA+DQYE + + +A + Sbjct: 41 SASGKKLSDPVLSKRMELYHKIEAVTQIPWYALAAVDQYEENVRSNRKDLPEKAG--IIS 98 Query: 102 ISFPPPVWSGPLNPNQEDQSPESIRFFXXXXXXXXXXXXADPENDADVLYSMARHLMKYG 161 I P +WSGP NPN +D +P SI+ F A+ ND D+LY+ +++L+ YG Sbjct: 99 IYIPDDIWSGPENPNPKDDAPLSIKVFDGIGMDGDGDGKAEVSNDEDILYTFSQYLLSYG 158 Query: 162 DSSSDFNIGVWEYYHNGRATQRIEQFARLYEHFGRLDLSGSAFPLPVGKTYSYRSTWGTG 221 + IG+W YY + I +F +L++ +G +DL AFPLP+ YSYRSTWG Sbjct: 159 TDEDNIRIGLWNYYRRDQTVGIISEFMKLFKAYGHIDLGEHAFPLPIRTDYSYRSTWGDA 218 Query: 222 RSWGGARIHEGTDLFTPYGMTVRSTCFGIVETKGWNRYGGWRIGIRDIENRYHYYAHLAG 281 R +GG RIHEGTD+F YG+ V+STC+G+VE KGWNR+GGWRIGIRDI N YHY+AHL G Sbjct: 219 RGFGGRRIHEGTDIFAHYGLPVKSTCYGVVEMKGWNRFGGWRIGIRDINNTYHYFAHLNG 278 Query: 282 FEKSLSRGDIVAPGQTIGWVGSSGYGNPGTQGKFPPHLHYGIYRDRGITEWAFDPYPLLK 341 F K + G IV PGQ IG VGSSGYG PGT GKFPPHLHYG+Y+D G TEW+FDPYP L+ Sbjct: 279 FAKGIKTGQIVEPGQVIGSVGSSGYGPPGTAGKFPPHLHYGMYKDNGRTEWSFDPYPHLR 338 Query: 342 QWETQEYRAMR 352 WE EY+ + Sbjct: 339 AWERYEYQKKK 349 >BH3436 [M] COG0739 Membrane proteins related to metalloendopeptidases Length = 337 Score = 321 bits (823), Expect = 1e-87, Method: Compositional matrix adjust. Identities = 170/345 (49%), Positives = 215/345 (62%), Gaps = 10/345 (2%) Query: 11 RKTVLCLSAAAMLWGDSDAVAGAPAGNKHAVSSEAASGKESLSVSRKQLYDQMSAATGIA 70 R ++ L+ ++L+ V P G A +S S +E R LY++M A T + Sbjct: 3 RIAIILLTLTSILF-----VQHVPEG--LAKASNELSLEEKYE-QRLTLYEKMEAITNVP 54 Query: 71 WFRFAAIDQYERTIAKKKSGTAGQAQPRLTGISFPPPVWSGPLNPNQEDQSPESIRFFXX 130 W+ AA+D YER + + + A+ L I F P W GPLNP+ ED +P SI F Sbjct: 55 WYYLAAVDTYERGLRRAQKDRP--AEEGLIAIYFSPQQWVGPLNPDLEDTNPLSISMFNG 112 Query: 131 XXXXXXXXXXADPENDADVLYSMARHLMKYGDSSSDFNIGVWEYYHNGRATQRIEQFARL 190 AD ND DVLY+ AR+L YG DF I +WEYY ++ I A + Sbjct: 113 VGLDGNGDGVADRNNDEDVLYTFARYLESYGFKEEDFRIALWEYYQREQSVNIIYGHANV 172 Query: 191 YEHFGRLDLSGSAFPLPVGKTYSYRSTWGTGRSWGGARIHEGTDLFTPYGMTVRSTCFGI 250 Y+ F LDL FPLP+ YSYRSTWG R WGG RIHEGTD+F Y + V +T +GI Sbjct: 173 YKTFKTLDLDDHTFPLPLHHNYSYRSTWGDRRGWGGRRIHEGTDIFANYNVPVMATAYGI 232 Query: 251 VETKGWNRYGGWRIGIRDIENRYHYYAHLAGFEKSLSRGDIVAPGQTIGWVGSSGYGNPG 310 VE KGWN+YGGWRIG+RD++N YHYYAHL+GFEK + +G +VAPG IG+VGSSGYG PG Sbjct: 233 VEIKGWNKYGGWRIGVRDLDNVYHYYAHLSGFEKGVEKGTVVAPGDVIGYVGSSGYGKPG 292 Query: 311 TQGKFPPHLHYGIYRDRGITEWAFDPYPLLKQWETQEYRAMRKHK 355 TQGKFPPHLHYG+YRD G TEW+FDPYP LK WE + Y+ +K K Sbjct: 293 TQGKFPPHLHYGMYRDNGYTEWSFDPYPSLKSWERKAYQERKKKK 337 >DR0598 [M] COG0739 Membrane proteins related to metalloendopeptidases Length = 197 Score = 80.5 bits (197), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 59/140 (42%), Positives = 74/140 (52%), Gaps = 15/140 (10%) Query: 202 SAFPLPV-GKTYSYRSTWGTGRSWGGARIHEGTDLFTPYGMTVRSTCFGIVETKGWNRYG 260 +A P P+ G+ + TWG RS G R HEG D+F P +R+T GIV G N G Sbjct: 67 TALPNPLPGRPLT--DTWGGARS--GGRKHEGIDIFAPRDTPIRATTEGIVLNVGPNTLG 122 Query: 261 GWRIGIRDIENRYHYYAHLAGFEKSLSRGDIVAPGQTIGWVGSSGYGNPGTQGKFPPHLH 320 G + I + HYYAHL + L+ G+ V G +G+VG SG GT P HLH Sbjct: 123 GRTVMILGPGGQRHYYAHLDRYP-DLTAGEWVNSGDVVGYVGDSGNAK-GT----PTHLH 176 Query: 321 YGIYRDRGITEWAFDPYPLL 340 YGIY G A +PYPLL Sbjct: 177 YGIYTAAG----AINPYPLL 192 >RC0557 [M] COG0739 Membrane proteins related to metalloendopeptidases Length = 476 Score = 58.5 bits (140), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 40/124 (32%), Positives = 55/124 (44%), Gaps = 12/124 (9%) Query: 205 PLPVGKTYSYRSTWGTGRS--WGGARIHEGTDLFTPYGMTVRSTCFGIVETKGWNRYGGW 262 PL V K S+ +G + G ++H G D P G + S G++ GW G Sbjct: 310 PLKVIKVSSH---YGNRKHPILGYTKMHRGVDFAAPTGTPIYSAGNGVITEIGWKSGYGK 366 Query: 263 RIGIRDIENRYHYYAHLAGFEKSLSRGDIVAPGQTIGWVGSSGYGNPGTQGKFPPHLHYG 322 I ++ YAH + F K+L G IV GQ I +VGS+G PHLHY Sbjct: 367 FIQVKHSGTLSTAYAHASNFAKNLKVGSIVKQGQIIAYVGSTGRAT-------APHLHYE 419 Query: 323 IYRD 326 + D Sbjct: 420 VKID 423 >BMEI0197 [M] COG0739 Membrane proteins related to metalloendopeptidases Length = 651 Score = 57.8 bits (138), Expect = 2e-08, Method: Compositional matrix adjust. Identities = 60/223 (26%), Positives = 93/223 (41%), Gaps = 42/223 (18%) Query: 143 PENDADVLYSMA------RHLMKYG--DSSSDFNIGVWEYYHNGRATQRIEQFARLYEHF 194 PEND+ +LY A R +Y D S D+ Y +G++ + QF L + Sbjct: 437 PENDSQLLYVAATFGGTTRKFYRYQAPDGSVDY------YNEDGKSAK---QF--LLRN- 484 Query: 195 GRLDLSGSAFPLPVGKTYSYRSTWGTGRS--WGGARIHEGTDLFTPYGMTVRSTCFGIVE 252 P+P G +RS +G R G R+H G D P G + + G+VE Sbjct: 485 ----------PVPNG---IFRSPFGMRRHPILGYTRMHTGVDWAAPRGTPIIAAGNGVVE 531 Query: 253 TKGWNRYGGWRIGIRDIENRYHYYAHLAGFEKSLSRGDIVAPGQTIGWVGSSGYGNPGTQ 312 GW+ G + IR Y+H + ++ G V GQ IG+VGS+G Sbjct: 532 KAGWSNGYGNQTLIRHANGYVTSYSHQNAIARGITPGARVRQGQVIGYVGSTGLST---- 587 Query: 313 GKFPPHLHYGIYRDRGITEWAFDPYPLLKQWETQEYRAMRKHK 355 PHLHY + + + P K + +E+ A ++ + Sbjct: 588 ---GPHLHYELIVNGTKVDALRIRLPDNKALKGKEFEAFKQER 627 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.432 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 28,219,867 Number of extensions: 1243677 Number of successful extensions: 3250 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 3259 Number of HSP's successfully gapped: 26 Length of query: 355 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 248 Effective length of database: 26,037,693 Effective search space: 6457347864 Effective search space used: 6457347864 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)