BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0030.p (365 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value all1694 [H] COG0320 Lipoate synthase 290 2e-78 BS_lipA [R] COG1075 Predicted acetyltransferases and hydrolases ... 288 7e-78 sll0868 [H] COG0320 Lipoate synthase 286 3e-77 slr1598 [H] COG0320 Lipoate synthase 279 5e-75 alr1282 [H] COG0320 Lipoate synthase 278 1e-74 >all1694 [H] COG0320 Lipoate synthase Length = 291 Score = 290 bits (743), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 143/279 (51%), Positives = 188/279 (67%), Gaps = 2/279 (0%) Query: 75 KPDWIKIKLTTGDNYQEIKGMMRSKTLHTVCEEARCPNIYECWANRTATFMILGDICTRA 134 KPDW+++K + +K ++R L+TVCEEA CPNI EC+ TATF+I+G CTRA Sbjct: 4 KPDWLRVKAPQWERVGNVKEILRDLALNTVCEEASCPNIGECFQAGTATFLIMGPACTRA 63 Query: 135 CRFCAVN-TGLPTELDLQEPERVAEAAENMKLKHCVVTSVARDDLKDGGARIFAETVAAI 193 C +C ++ P LD EP R+AEA M+L H V+TSV RDDL DGGA F + +A + Sbjct: 64 CPYCDIDFEKKPKALDPTEPTRLAEAVRRMRLNHVVITSVNRDDLPDGGASQFVKCIAEV 123 Query: 194 RKRLPLCSVEVLIPDFLGERDSLEIVMNSRPDILNHNIETVERMSDRVRARAKYRRSLEL 253 R P ++EVLIPD G ++L I++ + P++LNHN ET+ R+ RVR + Y R+LEL Sbjct: 124 RAVSPQTTIEVLIPDLCGNWEALRIILQASPEVLNHNTETIPRLYRRVRPQGNYDRTLEL 183 Query: 254 LSRAKEIKPDIPTKSSIMLGVGEEWDEILQAMDDLRAVDCDILTLGQYLQPSPKHLNVEK 313 L R+++I P + TKS IM+G+GE E+ Q M DLRAVDCDILT+GQYLQPS KHL V Sbjct: 184 LQRSRQIAPWLYTKSGIMVGLGETNAEVRQVMQDLRAVDCDILTIGQYLQPSQKHLQVND 243 Query: 314 FYNPEEFALLKEEGLKRGFSHVESGPLVRSSYHAHEQVK 352 F PE+FA + G + GF V S PL RSSYHA EQV+ Sbjct: 244 FVTPEQFAAWQVYGEELGFLQVVSSPLTRSSYHA-EQVR 281 >BS_lipA [R] COG1075 Predicted acetyltransferases and hydrolases with the alpha/beta hydrolase fold Length = 212 Score = 288 bits (738), Expect = 7e-78, Method: Compositional matrix adjust. Identities = 0/292 (0%), Positives = 0/292 (0%), Gaps = 2/292 (0%) Query: 64 TLTNNRTAKQPKPDWIKIKLTTGDN-YQEIKGMMRSKTLHTVCEEARCPNIYECWANRTA 122 + P I + + + + + + + + N Sbjct: 26 QPSAKAAEHNPVVMVHGIGGASFNFAGIKSYLVSQGWSRDKLYAVDFWDKTGTNYNNGPV 85 Query: 123 TFMILGDICTRACRFCAVNTGLPTELDLQEPERVAEAAENMKLKHCVVTSVARDDLKDGG 182 + + N + R Sbjct: 86 LSRFVQKVLDETGAKKVDIVAHSMGGANTLYYIKNLDGGNKVANVVTLGGANRLTTGKAL 145 Query: 183 ARIFAETVAAIRKRLPLCSVEVLIPDFLGERD-SLEIVMNSRPDILNHNIETVERMSDRV 241 + V+ + +++I +L + Sbjct: 146 PGTDPNQKILYTSIYSSADMIVMNYLSRLDGARNVQIHGVGHIGLLYSSQVNSLIKEGLN 205 Query: 242 RARAKYRRSLELLSRAKEIKPDIPTKSSIMLGVGEEWDEILQAMDDLRAVDCDILTLGQY 301 Sbjct: 206 265 Query: 302 LQPSPKHLNVEKFYNPEEFALLKEEGLKRGFSHVESGPLVRSSYHAHEQVKS 353 Sbjct: 266 317 >sll0868 [H] COG0320 Lipoate synthase Length = 303 Score = 286 bits (732), Expect = 3e-77, Method: Compositional matrix adjust. Identities = 142/282 (50%), Positives = 186/282 (65%), Gaps = 8/282 (2%) Query: 76 PDWIKIKLTTGDNYQEIKGMMRSKTLHTVCEEARCPNIYECWANRTATFMILGDICTRAC 135 P W+K + ++ +++ + +HT+CEE RCPN EC+ANRTATF+++G ICTRAC Sbjct: 21 PSWLKRPIGRASELSTVQQIIKQRQIHTICEEGRCPNRGECYANRTATFLLMGQICTRAC 80 Query: 136 RFCAVNTG-LPTELDLQEPERVAEAAENMKLKHCVVTSVARDDLKDGGARIFAETVAAIR 194 FC V G P LD EP +VAEA + + LK+ V+TSVARDDL DGGA F + IR Sbjct: 81 GFCQVEKGQAPMMLDQDEPRKVAEAVQLLGLKYVVLTSVARDDLTDGGAGWFVAVMERIR 140 Query: 195 KRLPLCSVEVLIPDFLG-------ERDSLEIVMNSRPDILNHNIETVERMSDRVRARAKY 247 + P +EVL PDF G +++ + V ++P NHN+ETV R+ +VR AKY Sbjct: 141 QDNPATQIEVLTPDFWGGMGRERSQKERVLTVTKAKPVCYNHNLETVARLQGKVRRGAKY 200 Query: 248 RRSLELLSRAKEIKPDIPTKSSIMLGVGEEWDEILQAMDDLRAVDCDILTLGQYLQPSPK 307 +RSL++L KE PDI TKS +MLG GE DE+++ + DLR+V CD LTLGQY+QPS Sbjct: 201 QRSLDVLRWIKEFDPDIFTKSGLMLGHGETVDEVVETLKDLRSVGCDRLTLGQYMQPSLD 260 Query: 308 HLNVEKFYNPEEFALLKEEGLKRGFSHVESGPLVRSSYHAHE 349 HL V+K++ PEEF L E GFSHV SGPLVRSSYHA E Sbjct: 261 HLPVQKYWTPEEFDQLGEIAKDLGFSHVRSGPLVRSSYHAGE 302 >slr1598 [H] COG0320 Lipoate synthase Length = 289 Score = 279 bits (713), Expect = 5e-75, Method: Compositional matrix adjust. Identities = 143/280 (51%), Positives = 189/280 (67%), Gaps = 2/280 (0%) Query: 75 KPDWIKIKLTTGDNYQEIKGMMRSKTLHTVCEEARCPNIYECWANRTATFMILGDICTRA 134 KPDW+++K +K ++R L+TVCEEA CPNI EC+ TATF+I+G CTRA Sbjct: 4 KPDWLRVKAPQWQRVGSVKDILRDLQLNTVCEEASCPNIGECFQAGTATFLIMGPACTRA 63 Query: 135 CRFCAVN-TGLPTELDLQEPERVAEAAENMKLKHCVVTSVARDDLKDGGARIFAETVAAI 193 C +C ++ P LD EPER+A A + + LKH V+T+V RDDL DGGA F + AI Sbjct: 64 CPYCDIDFEKKPQPLDSTEPERLAIAVKRLNLKHVVITAVNRDDLPDGGASQFVSCIEAI 123 Query: 194 RKRLPLCSVEVLIPDFLGERDSLEIVMNSRPDILNHNIETVERMSDRVRARAKYRRSLEL 253 RK P ++EVLIPD G +L ++ + P++LNHN+ETV R+ RVR +A Y +SLEL Sbjct: 124 RKISPGTTIEVLIPDLCGNWQALAALLEAAPEVLNHNMETVPRLYKRVRPQADYDQSLEL 183 Query: 254 LSRAKEIKPDIPTKSSIMLGVGEEWDEILQAMDDLRAVDCDILTLGQYLQPSPKHLNVEK 313 ++AK I P + TKS IM+G+GE E+ Q M+DLR VDCDILT+GQYLQPS KHL V++ Sbjct: 184 FNQAKRILPKVYTKSGIMVGLGETDAEVRQVMEDLRRVDCDILTIGQYLQPSQKHLGVKE 243 Query: 314 FYNPEEFALLKEEGLKRGFSHVESGPLVRSSYHAHEQVKS 353 F PE+F + + G GF V S PL RSSYHA EQV++ Sbjct: 244 FVTPEQFDVWRIYGESIGFLQVVSSPLTRSSYHA-EQVQA 282 >alr1282 [H] COG0320 Lipoate synthase Length = 359 Score = 278 bits (710), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 16/284 (5%), Positives = 43/284 (15%), Gaps = 9/284 (3%) Query: 76 PDWIKIKLTTGDNYQEIKGMMRSKTLHTVCEEARCPNIYECWANRTATFMILGDICTRAC 135 ++ +K L + + A + G I Sbjct: 17 LQRLRELAPASKMVAVVKANAYGHGLLETARTLPDADAFGVARLEEALRLRAGGITKPVL 76 Query: 136 RFCAVNTG-LPTELDLQEPERVAEAAENMKLKHCVVTSVARDDLKDGGARIFAETVAAIR 194 + Q E + + V + Sbjct: 77 LLEGFFDARDLPTISAQHFHTAVHNEEQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQ 136 Query: 195 KRLPLCSVEVLIPDFLG--------ERDSLEIVMNSRPDILNHNIETVERMSDRVRARAK 246 + D + + + + + + A Sbjct: 137 AEAFYHRLTQCKNVRQPVNIVSHFARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGG 196 Query: 247 YRRSLELLSRAKEIKPDIPTKSSIMLGVGEEWDEILQAMDDLRAVDCDILTLGQYLQPSP 306 + + S + M ++ Sbjct: 197 ILLWPQSHFDWVRPGIILYGVSPLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYG 256 Query: 307 KHLNVEKFYNPEEFALLKEEGLKRGFSHVESGPLVRSSYHAHEQ 350 E+ A+ +G R + + Sbjct: 257 GTWVSERDTRLGVVAMGYGDGYPRAAPSGTPVLVNGREVPIVGR 300 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,757,882 Number of extensions: 969238 Number of successful extensions: 3175 Number of sequences better than 1.0e-05: 7 Number of HSP's gapped: 3162 Number of HSP's successfully gapped: 7 Length of query: 365 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 257 Effective length of database: 25,922,359 Effective search space: 6662046263 Effective search space used: 6662046263 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)