BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0022.p (479 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1658 [GEPR] COG0477 Permeases of the major facilitator superf... 278 1e-74 lin0519 [GEPR] COG0477 Permeases of the major facilitator superf... 241 2e-63 BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superf... 218 1e-56 L132251 [GEPR] COG0477 Permeases of the major facilitator superf... 199 8e-51 BH0554 [GEPR] COG0477 Permeases of the major facilitator superfa... 154 4e-37 >lin1658 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 489 Score = 278 bits (711), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 161/488 (32%), Positives = 272/488 (55%), Gaps = 17/488 (3%) Query: 4 KVVEHAMKRKPVIIVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVYLLV 63 K ++KR ++IV +++G F+ L QT++N ALP +M D GI A++ QWL T ++LV Sbjct: 2 KAASTSVKRNGILIV--MLMGAFVTILNQTLMNVALPSIMKDFGITASQGQWLSTGFMLV 59 Query: 64 SGIVIPVTGFLIGKYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGLLIP 123 +G++IP+T FLI +++TR L+ ++ F GT I GF++++ ++ GR++Q +GAG+++P Sbjct: 60 NGVMIPMTAFLIERFTTRQLYLFAMITFAIGTAIGGFATDYTMLIAGRMVQAIGAGIVMP 119 Query: 124 VFLNTIIMVFPKEKIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIAIAN 183 + ++ +FP E+ G AMGL L + APA+GPT+SG++VQ + WR LFF + P AI + Sbjct: 120 LLTVVVLNLFPMERRGRAMGLIGLAMNFAPAIGPTLSGWIVQQYDWRNLFFIIIPFAILD 179 Query: 184 FFVAYYCLENVGETNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXXXXXXXGFI 243 VA + L+NVG+ KLD V+ ST+GFGSLL GFS G+ G + Sbjct: 180 IVVAIFLLKNVGKRTFPKLDILGVIMSTVGFGSLLLGFSNAGDHDWLTWKVAGFIILGLV 239 Query: 244 ITFLFVKRQFKLAVPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRGLMP 303 + +F++ Q PLL+F VF+YP F + I + + + G LLLPI+ Q VRG P Sbjct: 240 VLGIFIRYQSSNKAPLLNFKVFKYPTFALTTAISFFVVMGLFGGMLLLPIFLQTVRGFSP 299 Query: 304 RESXXXXXXXXXXXXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMTLTVHASFSL 363 ES + ++G ++DRFG ++L G I A S+ + L + + Sbjct: 300 LESGLVLLPGALVTAILSPVTGVMFDRFGAKYLSLVGLI-IMAGSTFMFTNLDESTTLTF 358 Query: 364 LMGIYALLMTGIGFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALLITLMTI 423 ++ + + G+ +M P+ A+ SLP + + S M +R ++ S+G A LIT+M+ Sbjct: 359 IIIVQTIRSAGMAMVMMPLQTAALNSLPLKLASHGSAMFNTMRQVAGSIGTAALITVMSK 418 Query: 424 T-ADYSSVSFPVN-------------MLEGIRAAFWTLTVIAAGGFFLSFFVPYAQGSAH 469 + A ++ P + ++ GI AF +++ L+ F+ + + Sbjct: 419 SAASFADKLGPADVIGKTKTEVANHVLIHGIETAFLVAGILSVVACILALFIQKNRSAMD 478 Query: 470 PQVKESRQ 477 P V ++ + Sbjct: 479 PIVTKTEE 486 >lin0519 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 471 Score = 241 bits (615), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 143/421 (33%), Positives = 231/421 (54%), Gaps = 3/421 (0%) Query: 3 QKVVEHAMKRKPVIIVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVYLL 62 Q+ K +P+ I+AS ++ F+ +T LN AL ++ I + QWL T YLL Sbjct: 2 QQEATGGQKIRPIPIIASFLMAGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYLL 61 Query: 63 VSGIVIPVTGFLIGKYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGLLI 122 GI++P++G L+ ++TR LFF +V GTLIA S FA ++IGR++Q VG LL+ Sbjct: 62 TLGILVPISGLLLQWFTTRGLFFTAVSFSIAGTLIAALSPTFAMLMIGRVVQAVGTALLL 121 Query: 123 PVFLNTIIMVFPKEKIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIAIA 182 P+ NTI+++FP+ K G+AMG+ L++ APA+GPTISG ++++ +W +F+ P I Sbjct: 122 PLMFNTILLIFPEHKRGSAMGMIGLVIMFAPAVGPTISGLILENLTWNWIFWISLPFLII 181 Query: 183 NFFVAYYCLENVGETNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXXXXXXXGF 242 ++NV K K+D S++ STLGFG +++ FS GE G G Sbjct: 182 ALLFGMKFMQNVSVVTKPKIDILSIILSTLGFGGVVFAFSSAGENGWGSATVLVSIIVGG 241 Query: 243 IITFLFVKRQFKLAVPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRGLM 302 I LFV RQ + PL+D VF+YP FT I+ I F+++ +LLP+Y QN L Sbjct: 242 IALGLFVWRQLTMEKPLMDLKVFKYPMFTLGLILVFISFMMILSTMILLPLYLQNSLALA 301 Query: 303 PRESXXXXXXXXXXXXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMT-LTVHASF 361 + + +G+++D +G R L+ GF I AV +L +T + V S Sbjct: 302 AFSAGLVLLPGGVLNGLMSPFTGRLFDAYGPRALVIPGF--IVAVIALFFLTRIEVGTSA 359 Query: 362 SLLMGIYALLMTGIGFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALLITLM 421 ++ ++++LM GI +M P + LP + + + ++ +S ++G A+ IT+M Sbjct: 360 LTIIVLHSVLMIGISMVMMPAQTNGLNQLPPKLYPDGTAIMNTLQQVSGAIGTAVAITIM 419 Query: 422 T 422 + Sbjct: 420 S 420 >BS_lmrB [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 479 Score = 218 bits (556), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 144/469 (30%), Positives = 241/469 (51%), Gaps = 12/469 (2%) Query: 3 QKVVEHAMKRKPVIIVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVYLL 62 + + + + K + I+ SL++ F+ +T LN AL +M +L I A QWL T YLL Sbjct: 4 ETTAKASQQYKVMPIMISLLLAGFIGMFSETALNIALTDLMKELNITAATVQWLTTGYLL 63 Query: 63 VSGIVIPVTGFLIGKYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGLLI 122 V GI++PV+G L+ ++TR LF S+ GT IA + F+F+L RI+Q +G GLL+ Sbjct: 64 VLGILVPVSGLLLQWFTTRQLFTVSLIFSILGTFIAALAPSFSFLLAARIVQALGTGLLL 123 Query: 123 PVFLNTIIMVFPKEKIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIAIA 182 P+ NTI+++FP K GAAMG L++ APA+GPT SG V++ +W +F+ P + Sbjct: 124 PLMFNTILVIFPPHKRGAAMGTIGLVIMFAPAIGPTFSGLVLEHLNWHWIFWISLPFLVL 183 Query: 183 NFFVAYYCLENVGETNKTKLDRRSVLYSTLGFGSLLYGFSILGE--QGRXXXXXXXXXXX 240 ++NV ET K K+D S++ ST+GFG +++GFS GE G Sbjct: 184 ALVFGIAYMQNVSETTKPKIDVLSIILSTIGFGGIVFGFSNAGEGSGGWSSPTVIVSLIV 243 Query: 241 GFIITFLFVKRQFKLAVPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRG 300 G + LF RQ + P+++ F+YP F I+ I +V+ LLLP+Y Q Sbjct: 244 GVVGLILFSIRQLTMKQPMMNLRAFKYPMFILGVIMVFICMMVILSSMLLLPMYLQGGLV 303 Query: 301 LMPRESXXXXXXXXXXXXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMTLTVHAS 360 L S ++G+++D++G + L+ GF + V + +T ++ Sbjct: 304 LTAFASGLVLLPGGILNGFMSPVTGRLFDKYGPKWLVIPGFVIVTVVLWFFS-NVTTTST 362 Query: 361 FSLLMGIYALLMTGIGFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALLITL 420 L++ ++ LM GI +M P + LP + + ++ ++ ++G A+ +++ Sbjct: 363 AVLIIILHTCLMIGISMIMMPAQTNGLNQLPREFYPDGTAIMNTLQQMAGAIGTAVAVSI 422 Query: 421 MTI-TADY-SSVSFPVN-------MLEGIRAAFWTLTVIAAGGFFLSFF 460 M DY S+V P + + G++ AF ++A G +FF Sbjct: 423 MAAGQHDYMSTVKNPADPAVIPQALTAGVQHAFVFAMIVAIIGLIGAFF 471 >L132251 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 514 Score = 199 bits (506), Expect = 8e-51, Method: Compositional matrix adjust. Identities = 132/411 (32%), Positives = 212/411 (51%), Gaps = 16/411 (3%) Query: 21 LIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVYLLVSGIVIPVTGFLIGKYST 80 ++VG F A LMQT L TA+P +M+D I + AQ T +LL +GI++P++ +L K+ T Sbjct: 27 ILVGTFGAMLMQTSLGTAIPTLMNDFNISMSTAQQATTWFLLANGIMVPISAYLATKFPT 86 Query: 81 RALFFASVGAFTTGTLIA--GFSSEFAFILIGRIIQGVGAGLLIPVFLNTIIMVFPKEKI 138 R L+ + G +A +S + L GRI+Q + G+ +P+ ++ +FP +++ Sbjct: 87 RWLYVTAYATLFIGMFVAYSAPTSSWWVFLAGRILQAIAVGITMPLMQVALVNMFPAKQM 146 Query: 139 GAAMGLASLIVGLAPAMGPTISGFVVQDH----------SWRILFFGVAPIAIANFFVAY 188 GA MG+A +++ LAPA+GPT +G+++ SWR +F + F ++ Sbjct: 147 GAIMGVAGVVLVLAPALGPTFAGWLITHKFNLIFWTFAASWRTIFLIPMIVVGVVFLISI 206 Query: 189 YCLENVGETNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXXXXXXXG-FIITFL 247 + + +V KLD SVL S +GFG+ L+GF+ + G G II FL Sbjct: 207 FAMRDVIPNRPMKLDFLSVLLSVVGFGAFLWGFTNVATDGWGKFNTVIAPIVGGLIIIFL 266 Query: 248 FVKRQFKLAVPLLDFNVFRYPRFTYASIIGVILFIVMAGVELLLPIYAQNVRGLMPRESX 307 F RQ K+ P LD VF+ +FT ++ + + M GVE++LP+Y Q+V GL S Sbjct: 267 FGLRQLKMDDPFLDIRVFKIRQFTLVTLALALSTMAMMGVEMMLPLYLQDVHGLSAFNSG 326 Query: 308 XXXXXXXXXXXVSGVISGKIYDRFGGRHLIRTGFFWIA-AVSSLLTMTLTVHASFSLLMG 366 V ++G +YDR G + L GF +A + T T F L+ Sbjct: 327 LVLLPGSLMMAVVAPLAGGVYDRIGSKRLAVIGFLVLALGTIPFMFFTATTPDHFDTLL- 385 Query: 367 IYALLMTGIGFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMGGALL 417 Y L M G+G +M P+T AM ++P + + A+ R ++ S+ ALL Sbjct: 386 -YGLRMVGVGMVMMPLTTSAMNAVPVNEVAQATASNNTARQVASSVVVALL 435 >BH0554 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 474 Score = 154 bits (388), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 104/403 (25%), Positives = 194/403 (48%), Gaps = 4/403 (0%) Query: 13 KPVIIVASLIVGNFLAQLMQTMLNTALPQMMSDLGIQANRAQWLITVYLLVSGIVIPVTG 72 K ++V +++ G+F L +MLN A+PQ+M+ A W+IT++++ G+ +P+TG Sbjct: 6 KKWMVVCAVLFGSFTMILNNSMLNPAIPQLMNVFEADAVATGWVITIFMVAMGMTMPLTG 65 Query: 73 FLIGKYSTRALFFASVGAFTTGTLIAGFSSEFAFILIGRIIQGVGAGLLIPVFLNTIIMV 132 +L K + + +G F G+L+ S +++ R +QG+G G+++P+ + I Sbjct: 66 YLGDKIGKKEAYILGLGIFVLGSLLGALSWNLPSLIVFRGLQGIGGGVMMPLSMTLIFDA 125 Query: 133 FPKEKIGAAMGLASLIVGLAPAMGPTISGFVVQDHSWRILFFGVAPIAIANFFVAYYCLE 192 FP+ + G A G+ + +AP +GPT+ GF+V+ +W+ LF P + A L Sbjct: 126 FPRNERGLATGVWGVASMMAPTIGPTLGGFIVETSNWKYLFLVNIPFGLLGIIAAVIYLP 185 Query: 193 NVGETNKTKLDRRSVLYSTLGFGSLLYGFSILGEQGRXXXXXX-XXXXXGFIITFLFVKR 251 + + + KLDR L+ T G GS+L F + + G + ++FV+ Sbjct: 186 KIARSGQIKLDRWGFLFVTAGVGSILLAFGRMSDLTHLTEWINGVLLLLGALCLYVFVQV 245 Query: 252 QFKLAVPLLDFNVFRYPRFTYA-SIIGVILFIVMAGVELLLPIYAQNVRGLMPRESXXXX 310 + + PLLD ++FR P ++ + + G+ + AG+ L+P+ Q V P + Sbjct: 246 EKRAEQPLLDLSLFRIPAYSLSIWVAGISSIGLFAGI-FLVPLLLQQVYDYGPIMTGLVF 304 Query: 311 XXXXXXXXVSGVISGKIYDRFGGRHLIRTGFFWIAAVSSLLTMTLTVHASFSLLMGIYAL 370 ++ I G++ D+ G ++ G IAAV + L + + A+ Sbjct: 305 LPSALFTGLTMSIGGRMLDKRGPSGIMTAGMM-IAAVGTFALGYLHLETGLWYIFAWMAI 363 Query: 371 LMTGIGFMMTPITALAMASLPNSIMKYASPMTTAIRTLSMSMG 413 G+G P T M ++P ++ S M +R +S + G Sbjct: 364 RGVGMGLTTMPATTTGMNAIPEGLISRGSAMNNVLRQMSSAFG 406 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.329 0.142 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 30,834,400 Number of extensions: 1232686 Number of successful extensions: 5206 Number of sequences better than 1.0e-05: 107 Number of HSP's gapped: 5136 Number of HSP's successfully gapped: 107 Length of query: 479 Length of database: 38,378,431 Length adjustment: 110 Effective length of query: 369 Effective length of database: 25,691,691 Effective search space: 9480233979 Effective search space used: 9480233979 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 118 (50.1 bits)