BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0018.p (267 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_yjbN [G] COG0061 Predicted sugar kinase 285 7e-77 BH2848 [G] COG0061 Predicted sugar kinase 283 2e-76 lin0967 [G] COG0061 Predicted sugar kinase 260 2e-69 BS_ytdI [G] COG0061 Predicted sugar kinase 140 2e-33 BH3199 [G] COG0061 Predicted sugar kinase 130 2e-30 >BS_yjbN [G] COG0061 Predicted sugar kinase Length = 345 Score = 285 bits (728), Expect = 7e-77, Method: Compositional matrix adjust. Identities = 12/268 (4%), Positives = 46/268 (17%), Gaps = 8/268 (2%) Query: 1 LRYYVLDRGDELSIKLAEQFHKLAAERNLELDAKSPEIVISIGGDGTMLHAFHTFIDQIP 60 + + + + E+ + R + + + + Sbjct: 1 MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVT 60 Query: 61 NLAFVGVHTGHLGFYADWKADELPALADYMCGTGSAEPHKPRIVKYPLLELEI--HKKSG 118 A + +L + + L + Sbjct: 61 TGAIIHGKGDYLAYSEEEHPVALQLGGSDPAAL----AQ--CAKLAEARGYDEINLNVGC 114 Query: 119 SSSHIALNEFTLKGVDGTVVIQVDINDVTFEMFRGDGLCVSTPSGSTAYNKSLGGAMVHP 178 S + F + ++ + + + + + L + Sbjct: 115 PSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV 174 Query: 179 TIEALQIAEIASINNRVFRTMGSPLLLPKHHHCDIFSRKDQRLLLTIDHNNIPVDDLISV 238 + + I + + +R + + + Sbjct: 175 SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEE 234 Query: 239 RCQVSDKKISFARYRPFPFWNRVREAFL 266 R + A Sbjct: 235 AKAHLQHMDGVMVGREAYQNPGILAAVD 262 >BH2848 [G] COG0061 Predicted sugar kinase Length = 265 Score = 283 bits (724), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 134/268 (50%), Positives = 194/268 (72%), Gaps = 8/268 (2%) Query: 1 LRYYVLDRGDELSIKLAEQFHKLAAERNLELDAKSPEIVISIGGDGTMLHAFHTFIDQIP 60 +R+ V RGD++S L ++ + + L LD + P+IVI++GGDGT+LHAFH + ++ Sbjct: 1 MRFTVTSRGDDVSNTLQQKIKRYLLDFGLTLDEQEPDIVITVGGDGTLLHAFHRYTSRLE 60 Query: 61 NLAFVGVHTGHLGFYADWKADELPALADYMCGTGSAEPHKPRIVKYPLLELEIHKKSGSS 120 + AFVG+HTGHLGFYADW DE+ L ++ T P++ IV+YPLLE+ + S Sbjct: 61 DTAFVGIHTGHLGFYADWVPDEVEKLVIHIAKT----PYQ--IVEYPLLEVVVRHTDESE 114 Query: 121 SH--IALNEFTLKGVDGTVVIQVDINDVTFEMFRGDGLCVSTPSGSTAYNKSLGGAMVHP 178 S +ALNE T+K +G++V V+I FE+FRGDGLC+STPSGSTAYNK+LGGA++HP Sbjct: 115 SKRLLALNECTVKSQEGSLVSNVEIKGDVFEVFRGDGLCISTPSGSTAYNKALGGAILHP 174 Query: 179 TIEALQIAEIASINNRVFRTMGSPLLLPKHHHCDIFSRKDQRLLLTIDHNNIPVDDLISV 238 ++ ++QI+E+ASINNRV+RT+GSPL+LP+HH C I L +TIDH + + S+ Sbjct: 175 SLASIQISEMASINNRVYRTIGSPLVLPQHHTCLIKPLNQVELQVTIDHFTLAYKRVKSI 234 Query: 239 RCQVSDKKISFARYRPFPFWNRVREAFL 266 +C+V+++KI FAR+RPFPFW RV+E+F+ Sbjct: 235 QCRVAEEKIRFARFRPFPFWKRVKESFI 262 >lin0967 [G] COG0061 Predicted sugar kinase Length = 264 Score = 260 bits (664), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 130/268 (48%), Positives = 181/268 (67%), Gaps = 8/268 (2%) Query: 1 LRYYVLDRGDELSIKLAEQFHKLAAERNLELDAKSPEIVISIGGDGTMLHAFHTFIDQIP 60 ++Y + +GDE S L E ++E D PEIVISIGGDGT L AFH + +++ Sbjct: 1 MKYMITSKGDEKSDLLRLNMIAGFGEYDMEYDEIEPEIVISIGGDGTFLSAFHQYEERLD 60 Query: 61 NLAFVGVHTGHLGFYADWKADELPALADYMCGTGSAEPHKPRIVKYPLLELEIHKKSG-- 118 +AF+G+HTGHLGFYADW+ PA AD + + ++ V YPLL+ + G Sbjct: 61 EIAFIGIHTGHLGFYADWR----PAEADKLVKLLAKGEYQK--VSYPLLKTTVKYGIGKK 114 Query: 119 SSSHIALNEFTLKGVDGTVVIQVDINDVTFEMFRGDGLCVSTPSGSTAYNKSLGGAMVHP 178 + ++ALNE T+K G V+ V IND+ FE FRGDGLC+STPSG+TAYNKSLGGA++HP Sbjct: 115 EAEYLALNESTVKSSGGPFVVDVVINDLHFERFRGDGLCMSTPSGTTAYNKSLGGALMHP 174 Query: 179 TIEALQIAEIASINNRVFRTMGSPLLLPKHHHCDIFSRKDQRLLLTIDHNNIPVDDLISV 238 +IEA+Q+ E+ASINNRV+RT+GSPL+ PKHH + D+ +++DH +I D+ + Sbjct: 175 SIEAMQLTEMASINNRVYRTIGSPLVFPKHHVVSLQPVNDKDFQISVDHLSILHRDVQEI 234 Query: 239 RCQVSDKKISFARYRPFPFWNRVREAFL 266 R +VS KK+ FAR+R FPFW RV ++F+ Sbjct: 235 RYEVSAKKVHFARFRSFPFWRRVHDSFI 262 >BS_ytdI [G] COG0061 Predicted sugar kinase Length = 267 Score = 140 bits (353), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 86/271 (31%), Positives = 146/271 (53%), Gaps = 20/271 (7%) Query: 4 YVLDRGDELSIKLAEQFHKLAAERNLEL--DAKSPEIVISIGGDGTMLHAFH--TFIDQI 59 Y + D+ + + A +LA E + I+ SIGGDGT L A F D Sbjct: 9 YFFHKQDQETNEQARSLTQLAEEHGFTVVNQHSDANIIASIGGDGTFLQAVRKTNFRD-- 66 Query: 60 PNLAFVGV-HTGHLGFYADWKADELPALADYMCGTGSAEPHKPRIVKYPLLELEIHKKSG 118 + +VG+ G Y D+ +DE + D M + + KYPL+E+ + + Sbjct: 67 -DCLYVGITKKGKAHLYCDFHSDEREKMVDAMTF------EQIEVRKYPLIEVTVDQ--- 116 Query: 119 SSSHIALNEFTLKG-VDGTVVIQVDINDVTFEMFRGDGLCVSTPSGSTAYNKSLGGAMVH 177 +S LNE +++ + T V+ V I+D+ FE FRGDG+ +STP+GSTAYNKS+ GA+V Sbjct: 117 ASPFHCLNEVSIRSSIIKTFVMDVLIDDLHFETFRGDGMIISTPTGSTAYNKSVAGAVVD 176 Query: 178 PTIEALQIAEIASINNRVFRTMGSPLLLPKHHHCDIFSRKD--QRLLLTIDHNNIPVDDL 235 P + +Q++E+AS+NN +RT+GSP +L + +D + ++ +D+ + ++ Sbjct: 177 PLLPCMQVSELASLNNNTYRTLGSPFVLSSDRKLTLRVVQDGNEHPIIGLDNEALSTRNV 236 Query: 236 ISVRCQVSDKKISFARYRPFPFWNRVREAFL 266 ++ ++S+KKI + + FW +V+ FL Sbjct: 237 KTIEIKLSNKKIKTVKLKDNSFWEKVKRTFL 267 >BH3199 [G] COG0061 Predicted sugar kinase Length = 265 Score = 130 bits (326), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 84/263 (31%), Positives = 140/263 (53%), Gaps = 31/263 (11%) Query: 17 AEQFHKLAAERNLEL--DAKSPEIVISIGGDGTMLHAFHTFIDQIPNLAFVGVHTGHLGF 74 E +LA + L + D + I++S+GG+ L A + + +VGV T GF Sbjct: 21 VEPLKQLATSQGLNVVDDVQKANIIVSVGGNNAFLQATRK-TNFRSDCLYVGVSTDREGF 79 Query: 75 YADWKADELPALADYMCGTGSAEPHKPRIVKYPLLELEIHKKSGSSSHIALNEFTLK-GV 133 Y D+ +E+ + + + E + + LE+ I + LNE +++ V Sbjct: 80 YPDFTINEIDKMFE------AFENQNIEVKRLSTLEVTIDDEK---PFYCLNECSIRSNV 130 Query: 134 DGTVVIQVDINDVTFEMFRGDGLCVSTPSGSTAYNKSLGGAMVHPTIEALQIAEIASINN 193 T V++V I+D+ FE FRGDG+ VSTP+GSTAYNKS+ GA+V P + +LQ++EIAS+NN Sbjct: 131 IKTFVLEVFIDDMHFETFRGDGMIVSTPTGSTAYNKSVRGAVVDPRLPSLQVSEIASLNN 190 Query: 194 RVFRTMGSPLLLPKHHHCDIFSRKDQRLLLTIDHNNIPV----DDLISVR------CQVS 243 +RT+G+ LL RL + D N+ P+ ++ +S+R ++S Sbjct: 191 NTYRTLGTSFLLSGDRTL--------RLKVVQDGNDFPIIGADNEALSIRHAEDIKIRLS 242 Query: 244 DKKISFARYRPFPFWNRVREAFL 266 DK++ + + FW++V+ FL Sbjct: 243 DKQVKVLKLKDNTFWHKVQRNFL 265 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.139 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,827,006 Number of extensions: 806236 Number of successful extensions: 1503 Number of sequences better than 1.0e-05: 17 Number of HSP's gapped: 1486 Number of HSP's successfully gapped: 17 Length of query: 267 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 162 Effective length of database: 26,268,361 Effective search space: 4255474482 Effective search space used: 4255474482 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 115 (48.9 bits)