BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0420.0006.p (329 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BH2870 [J] COG0180 Tryptophanyl-tRNA synthetase 382 e-106 STM3481 [J] COG0180 Tryptophanyl-tRNA synthetase 317 2e-86 SPAC3G9.13c [J] COG0180 Tryptophanyl-tRNA synthetase 234 1e-61 DR0558 [J] COG0180 Tryptophanyl-tRNA synthetase 222 8e-58 YDR268w [J] COG0180 Tryptophanyl-tRNA synthetase 207 1e-53 >BH2870 [J] COG0180 Tryptophanyl-tRNA synthetase Length = 346 Score = 382 bits (982), Expect = e-106, Method: Compositional matrix adjust. Identities = 11/325 (3%), Positives = 44/325 (13%) Query: 1 MAKKVLSGIQPSGSLTLGNYIGAIKNFVKLQEEHECYFMVVDLHAITVSQDPAALRENSE 60 M + S + + + + + V + + + E Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 61 SVAALYLAAGIDPKISNVYMQSHVQQHAELGWIMTTLTAMGELERMTQFKDKSSGKDSVG 120 + G + + + + ++ Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 121 AGLFVYPSLMAADILVYNADLVPVGEDQKQHLELTRDLAGRFNHRFGDFFTVPEPYIPEV 180 +G L Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSN 180 Query: 181 GARIMSLDDGTKKMSKSNPNPGSYIALLDPPDVIRKKISRATTDSGREVIFDPANKPEIS 240 + P + + + + + + + Sbjct: 181 ETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGT 240 Query: 241 NLMSIYAECSGMTLQQIADRYEGQMYGGFKKDLGEVLVAALEPLQQKYREIRSSGALSDI 300 + + G + V ++ + Sbjct: 241 LTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQP 300 Query: 301 LXXXXXXXXXXXXQTLAGVKEKMGF 325 Q ++ Sbjct: 301 FVASTLLGATTMDQLKTNIESLHLE 325 >STM3481 [J] COG0180 Tryptophanyl-tRNA synthetase Length = 346 Score = 317 bits (811), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 22/326 (6%), Positives = 50/326 (15%), Gaps = 4/326 (1%) Query: 5 VLSGIQPSGSLTLGNYIGAIKNFVKLQEEHECYFMVVDLHAITVSQDPAALRENSESVAA 64 + +L LG +N Y + ++ I V++ Sbjct: 6 IPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLT 65 Query: 65 LYLAAGIDPKISNVYMQSHVQQHAELGWIMTTLTAMGELERMTQFKDKSSG-KDSVGAGL 123 K + + + + ++ + Sbjct: 66 ETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDY 125 Query: 124 FVYPSLMAADILVYNADLVPVGEDQKQHLELTRDLAGRFNHRFGDFFTVPEPYIPEVGAR 183 + + D E Sbjct: 126 LDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFG 185 Query: 184 IMSLDDGTKKMSKSNPNPGSYIALLDPPDVIRKKISRATTDSGRE--VIFDPANKPEISN 241 +M K L + + Sbjct: 186 VMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKY 245 Query: 242 LMSIYAECSGMTLQQIADRYEGQMYGGFKKDLGEVLVAALEPLQQKYREIRSSGA-LSDI 300 L + TL RY G+ ++ Q ++ Sbjct: 246 LNGAKPAGARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVAST 305 Query: 301 LXXXXXXXXXXXXQTLAGVKEKMGFL 326 L ++ L Sbjct: 306 LLGATTMDQLKTNIESLHLELSEDVL 331 >SPAC3G9.13c [J] COG0180 Tryptophanyl-tRNA synthetase Length = 346 Score = 234 bits (597), Expect = 1e-61, Method: Compositional matrix adjust. Identities = 21/316 (6%), Positives = 50/316 (15%), Gaps = 23/316 (7%) Query: 4 KVLSGIQPSGSLTLGNYIGAIKNFVKLQEE------HECYFMVVDLHAITVSQDPAALRE 57 + G G A ++ Q Sbjct: 15 TLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLA 74 Query: 58 NSESVAALYLAAGIDPKISNVYMQSHVQQHAELGWIMTTLTAMGELERMTQFKDK----- 112 S L +A+ + N Q + I L + + Sbjct: 75 KHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWP 134 Query: 113 ----------SSGKDSVGAGLFVYPSLMAADILVYNADLVPVGEDQKQHLELTRDLAGRF 162 + + +L A + +G + + R L Sbjct: 135 QRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLAD 194 Query: 163 NHRFGD-FFTVPEPYIPEVGARIMSLDDGTKKMSKSNPNPGSYIALLDPPDVIRKKISRA 221 H Y + + L + ++ + Sbjct: 195 KHDLPRIVTIQNP-YSLLNRSFEVGLAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAG 253 Query: 222 TTDSGREVIFDPANKPEISNLMSIYAECSGMTLQQIADRYEGQMYGGFKKDLGEVLVAAL 281 ++ + + + + L F Sbjct: 254 ARNTLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMD 313 Query: 282 EPLQQKYREIRSSGAL 297 + Sbjct: 314 QLKTNIESLHLELSED 329 >DR0558 [J] COG0180 Tryptophanyl-tRNA synthetase Length = 346 Score = 222 bits (565), Expect = 8e-58, Method: Compositional matrix adjust. Identities = 24/337 (7%), Positives = 51/337 (15%), Gaps = 21/337 (6%) Query: 4 KVLSGIQPSGSLTLGNYIGAIKNFVKLQEEH--ECYFMVVDLHAITV--SQDPAALRENS 59 TLG + H Y + ++ I V E Sbjct: 3 YHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQ 62 Query: 60 ESVAALYLAAGIDPKISNVYMQSHVQQHAELGWIMTTLTAMGELERMTQFKDKSSGKDSV 119 + + + + S K Sbjct: 63 GLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQ 122 Query: 120 GAGLFVYPSLMAADILVYNADLVPVGEDQKQHLELTRDLAGRFNHRFGDFFTVPEPYIPE 179 L +Y L D + L L ++ Sbjct: 123 TDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNET 182 Query: 180 VGARIMSLDDGTKKMSKSNPNPGSYIALLDPPDVIRKKISRATTDSGREVIFDPA--NKP 237 + L K I ++ A V Sbjct: 183 AFGVMRYL-HLADKHDLPR---IVTIQNPYSLLNRSFEVGLAEVSQYEGVELLAYSCLGF 238 Query: 238 EISNLMSIYAECSGMTLQQIADRYEGQMYGGF-----KKDLGEVLVAALEPLQQKYREIR 292 + + + + +K + + A + Sbjct: 239 GTLTGKYLNGAKP--AGARN---TLFSRFTRYSGEQTQKAVAAYVDIARRHGLDPAQMAL 293 Query: 293 SS-GALSDILXXXXXXXXXXXXQTLAGVKEKMGFLPL 328 + + +T + Sbjct: 294 AFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDV 330 >YDR268w [J] COG0180 Tryptophanyl-tRNA synthetase Length = 346 Score = 207 bits (528), Expect = 1e-53, Method: Compositional matrix adjust. Identities = 0/347 (0%), Positives = 0/347 (0%), Gaps = 30/347 (8%) Query: 5 VLSGIQPSGSLTLGNYIGAIKNFVKL----QEEHECYFMVVDLHAITVSQ-DPAALRENS 59 + Q + + + ++ R N Sbjct: 37 DYAVAQGINLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNND 96 Query: 60 ESVAALYLAAGIDPKISNVYMQSHVQQHAELGWIMTTLTAMGELERMTQFKDKSSGKDS- 118 + + + + + +Q + + + S Sbjct: 97 KGIRPDQALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVS 156 Query: 119 -------------VGAGLFVYPSLMAADILVYNADLVPVGEDQKQHLELTRDLAGRFNHR 165 G ++ S A ++ L + + + Sbjct: 157 LLDTLDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFE 216 Query: 166 F-GDFFTVPEPYIPEVGARIMSLDDGTKKMSKSNPNPGSYIALLDPPDVIRKKISRATTD 224 + E + T K G+ L + ++ Sbjct: 217 VGLAEVSQYEGVELLA-YSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVA 275 Query: 225 S-GREVIFDPANKPEISNLMSIYAECSGMTLQQIADRYEG----QMYGGFKKDLGEVLVA 279 + + +++ + +L E ++A Sbjct: 276 AYVDIARRHGLDPAQMALAFVR---RQPFVASTLLGATTMDQLKTNIESLHLELSEDVLA 332 Query: 280 ALE-PLQQKYREIRSSGALSDILXXXXXXXXXXXXQTLAGVKEKMGF 325 +E Sbjct: 333 EIEA 379 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,493,817 Number of extensions: 1039509 Number of successful extensions: 1718 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 1702 Number of HSP's successfully gapped: 10 Length of query: 329 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 222 Effective length of database: 26,037,693 Effective search space: 5780367846 Effective search space used: 5780367846 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)