BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0419.0002.p (356 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DR2076 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 155 1e-37 RSc0281 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 129 5e-30 CC0395 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 125 1e-28 AF0534 [R] COG0491 Zn-dependent hydrolases, including glyoxylases 99 1e-20 VNG0709C [R] COG0491 Zn-dependent hydrolases, including glyoxylases 95 2e-19 >DR2076 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 347 Score = 155 bits (391), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 109/304 (35%), Positives = 151/304 (49%), Gaps = 14/304 (4%) Query: 25 GGIIQFSVPMAPPLRQVNSYILPDRNGQLTVIDPGPHTPEAEAAWEAVLQELDCSWSTIR 84 G + VP+ P++ V I G LT+ID TPEA AA E L +L WS I Sbjct: 33 GSLHALQVPIPYPMKTVTVLIDAPARGPLTLIDCALDTPEARAAIEDGLAQLGRHWSDID 92 Query: 85 DIVVTHHHPDHYGLAGWLQARSGGKVWMSERAHEEARLTWGAEGMLNEALP---LFFLRY 141 ++VTHHHPDHYGLAG ++ RSG +V M + W ML+E LP + Sbjct: 93 RLIVTHHHPDHYGLAGVIEERSGARVQMLDVEIGRGERYW---HMLSEWLPGHRKHMRDH 149 Query: 142 GMPEDWVQGIREHLESFLPQVTPQPEVAYINAAEPFIMGNRKWKPLVTGGHAPGHLSFYH 201 G+PE V+GIR + P+V P V + + + +W+ L GHA GHL ++ Sbjct: 150 GLPEASVEGIR----AENPRVHPASRVEPLREGQSVSLAGHEWEVLWLPGHADGHLGLWN 205 Query: 202 GGXXXGLILCGDAVLPQISPNVSLQPGSDPQPLRTFLEGLRELRSLRVTRAFPGHREP-F 260 L++ GDA+LP+ISPN+ L + P PL +L+ L +L +L RA GH P Sbjct: 206 --EEESLLIAGDAILPRISPNIGLYAYTRPDPLGDYLQTLGKLEALNPRRAVVGHHGPVM 263 Query: 261 TGFTERADSLLRHHEERLEQXXXXXXXXXXXXXXVCEALFRSRVSSAHQMRFAMSEALAH 320 G RA L HH ERL+ + A+F + + RFA++E LAH Sbjct: 264 EGVQARARELRGHHHERLDFIGAEARREGRSAYDLSLAMFPRDLDEGGR-RFALAETLAH 322 Query: 321 LAEL 324 L L Sbjct: 323 LEHL 326 >RSc0281 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 359 Score = 129 bits (325), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 106/337 (31%), Positives = 156/337 (46%), Gaps = 24/337 (7%) Query: 26 GIIQFSVPMAPPLRQVNSYILPDR-NGQ--LTVIDPGPHTPEAEAAWEAVLQELDCSWST 82 GI +P+ L +N ++L DR +GQ T++D G + A WE + + Sbjct: 28 GIYWLRMPLPFALDHINLWLLRDRQDGQDGWTIVDCGINHEAIRAYWETLFADGLDGLPV 87 Query: 83 IRDIVVTHHHPDHYGLAGWL-----QARSGGKVWMSERAHEEARLTWGAEGMLN---EAL 134 +R ++VTH HPDH GL+ WL R ++WMS + AR+ G G N E+ Sbjct: 88 LR-VLVTHCHPDHVGLSRWLCEGGDHQRWNVRLWMSLGDYAFARMLVGGTGASNAGGESA 146 Query: 135 PLFFLRYGMPE-DWVQGIREHLESFLPQVTPQPE-VAYINAAEPFIMGNRKWKPLVTGGH 192 F R+G+ + + V+ IR + + V PE I + +G +W+ + GH Sbjct: 147 ARHFARHGLTDAEAVERIRNRKDYYSTLVPGVPEQYRRIMDGQVIAIGPHRWRVITGFGH 206 Query: 193 APGHLSFYHGGXXXGLILCGDAVLPQISPNVS---LQPGSDPQPLRTFLEGLRELRSL-R 248 AP H++ Y G+++ GD VLP+IS NVS ++P +D PL +L L + +L Sbjct: 207 APEHVALY--SEDAGVLISGDMVLPRISTNVSVFDIEPEAD--PLTLYLTSLDKYEALPE 262 Query: 249 VTRAFPGHREPFTGFTERADSLLRHHEERLEQXXXXXXXXXXXXXXVCEALFRSRVSSAH 308 P H PF G R L HH +RL + + +F+ R H Sbjct: 263 DVLILPSHGRPFRGLHTRIRQLRDHHADRLADTLAACRRAPSSARDIVDVIFK-RQFDVH 321 Query: 309 QMRFAMSEALAHLAELVRRERAEVTGPEPGGVTMFAA 345 QM FAM EALAHL L R V P G+ F A Sbjct: 322 QMTFAMGEALAHLHALWHRGEV-VRAPGADGIIRFHA 357 >CC0395 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 317 Score = 125 bits (313), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 99/326 (30%), Positives = 146/326 (44%), Gaps = 16/326 (4%) Query: 32 VPMAPPLRQVNSYILPDRNGQLTVIDPGPHTPEAEAAWEAVLQELDCSWSTIRDIVVTHH 91 +P+ L+ +N + + D G V+D G T E AW A E I ++VTH Sbjct: 1 MPLGGSLQFINVWAIEDGEG-WAVVDTGVQTRETSQAWRAAFAEA-LGGKPITRVIVTHL 58 Query: 92 HPDHYGLAGWLQARSGGKVWMSERAHEEARLTWGAEGM-LNEALPLFFLRYGMPEDWVQG 150 HPDH GLAGW+ + ++WM+ + + R+ G E FF G ED ++ Sbjct: 59 HPDHIGLAGWMTRKFDCRLWMTRLEYFQCRMLVADTGREAPEDGVRFFRAAGWDEDAIEN 118 Query: 151 IREHLESFLPQVTPQPE-VAYINAAEPFIMGNRKWKPLVTGGHAPGHLSFYHGGXXXGLI 209 + F + P+ ++ E F +G R W+ + GH+P H + L+ Sbjct: 119 YKARFGGFGKAIYALPDSYRRLSDGEDFDIGGRTWRVVTGQGHSPDHACLWC--PELNLL 176 Query: 210 LCGDAVLPQISPNVSLQPGS-DPQPLRTFLEGLRELRSL--RVTRAFPGHREPFTGFTER 266 + GD VLP+IS NVS+ P D PL +L L ++++ P H +PF G R Sbjct: 177 ISGDQVLPRISSNVSVFPTEPDADPLSDWLRSLAKVKATVPDEVIVLPAHNDPFEGLHTR 236 Query: 267 ADSLLRHHEERLEQXXXXXXXXXXXXXXVCEALFRSRVSSAHQMRFAMSEALAHLAELVR 326 D+L+ HE L + ALF +R + A EALAHL L+R Sbjct: 237 IDALISGHERGLARLEKKLSERKRVVDTF-GALF-ARPIGPDLLGMATGEALAHLNCLIR 294 Query: 327 RERA--EVTGPEPGGVTMFAAVTQAA 350 R R EV G GV +AA + A Sbjct: 295 RGRVKREVDGD---GVAWYAANSSEA 317 >AF0534 [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 319 Score = 99.0 bits (245), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 84/312 (26%), Positives = 139/312 (44%), Gaps = 28/312 (8%) Query: 26 GIIQFSVPM-APPLRQVNSYILPDRNGQLTVIDPGPHTPEAEAAWEAVLQELDCSWSTIR 84 GI + VP+ PLR NSY++ D + L +ID G + + + L+EL+ +R Sbjct: 8 GIYRIEVPLKGSPLRYTNSYLVMDGDEAL-LIDTGFNRESCYRSIKGALEELN-----VR 61 Query: 85 D--IVVTHHHPDHYGLAGWLQARSGGKVWMSERAHEEARLTWGAEGMLNEALPLFFLRYG 142 + + TH H DH GLA A+ +V M E + R T E + L F+++ G Sbjct: 62 NFRVFCTHMHADHMGLA----AKLADEVLMGETEAKIVRETMVEEDYWRDLLK-FYIKNG 116 Query: 143 MPEDWVQGIREHLESFLP--QVTPQPEVAYINAAEPFIMGNRKWKPLVTGGHAPGHLSFY 200 P + I S P + +V ++ E +G+ + K + T GH+PGH Y Sbjct: 117 FPAKEAEKIL----SLHPGRNFWVKDDVEFVAVGEVVRVGSFRLKAIETPGHSPGHTCLY 172 Query: 201 HGGXXXGLILCGDAVLPQISPNVSLQPGSDPQPLRTFLEGLRELRSLRVTRAFPGHREPF 260 + GD VL I+PN++ P + L ++L+ L ++ +L V PGHR + Sbjct: 173 --DESRKIFFSGDHVLIDITPNITWWPFLE-DSLSSYLQSLEKVENLDVKLVLPGHRRRW 229 Query: 261 TGFTERADSLLRHHEERLEQXXXXXXXXXXXXX---XVCEALFRSRVSSAH--QMRFAMS 315 R + + +HH +RL + + L ++ + H Q FA+ Sbjct: 230 NDLKTRVEEIKKHHSKRLREALSALNTPKTAWEVAPSITWDLVYTKWADVHVVQKWFAVG 289 Query: 316 EALAHLAELVRR 327 E +AHL L + Sbjct: 290 ETIAHLEHLYHK 301 >VNG0709C [R] COG0491 Zn-dependent hydrolases, including glyoxylases Length = 328 Score = 95.1 bits (235), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 82/302 (27%), Positives = 135/302 (44%), Gaps = 9/302 (2%) Query: 29 QFSVPMAPPLRQVNSYILP--DRNGQLTVIDPGPHTPEAEAAWEAVLQELDCSWSTIRDI 86 QF + A + + Y+L D T++D G TP EA L + S++ I I Sbjct: 12 QFQLTNAAFEGRNSVYLLGVGDDAAPTTLVDTGIATPAVEAELRTALDDRGTSFAAIDRI 71 Query: 87 VVTHHHPDHYGLAGWLQARSGGKVWMSERAHEEARLTWGAEGMLNEAL--PLFFLRYGMP 144 ++TH H DH GLAG +QA V++ E + A + A+ + A + +GMP Sbjct: 72 LLTHWHHDHAGLAGTIQADGDATVYVHEA--DAAMVAQDADAVSGMAATQAAYLDEWGMP 129 Query: 145 EDWVQGIREHLESFLPQVTPQPEVAYINAAEPFIMGNRKWKPLVTGGHAPGHLSFYHGGX 204 D + + L++ +V ++ + +G + + GHA G +++ Sbjct: 130 ADKQAELNDFLDTHDGLQGQPADVTPLSDGDTVQLGGLDAEVVHLPGHAAGLVAYVVERD 189 Query: 205 XXGLILCGDAVLPQISPNVSLQPGSDPQPLRTFLEGLRELRSLRVTRAFPGHREPFTGFT 264 GDA+LP+ +PNV +PL+ + + L+ + +L + RA+PGHR P Sbjct: 190 GRREAFVGDAILPKYTPNVGGADVRVERPLQRYRDSLQRIEALDLDRAWPGHRGPVFAPA 249 Query: 265 ERADSLLRHHEERLEQXXXXXXXXXXXXX-XVCEALFRSRVSSAHQMRFAMSEALAHLAE 323 RA + HH+ER E+ V +ALF + H + EA AHL + Sbjct: 250 ARARDIAAHHDERTERVLDVVRARGPVSAWTVSDALF-GELHDIHVLH-GPGEAYAHLDD 307 Query: 324 LV 325 LV Sbjct: 308 LV 309 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.135 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,335,261 Number of extensions: 1197907 Number of successful extensions: 2090 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 2062 Number of HSP's successfully gapped: 15 Length of query: 356 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 249 Effective length of database: 26,037,693 Effective search space: 6483385557 Effective search space used: 6483385557 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)