BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0417.0006.p (501 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DR2626 [I] COG2272 Carboxylesterase type B 170 6e-42 DR0415 [I] COG2272 Carboxylesterase type B 157 5e-38 Rv2045c [I] COG2272 Carboxylesterase type B 142 2e-33 Rv1104 [I] COG2272 Carboxylesterase type B 116 7e-26 Cgl1109 [I] COG2272 Carboxylesterase type B 111 2e-24 >DR2626 [I] COG2272 Carboxylesterase type B Length = 540 Score = 170 bits (430), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 155/484 (32%), Positives = 216/484 (44%), Gaps = 62/484 (12%) Query: 16 ITSFKGIPFAAPPTGQNRWRAPQ--PASDWEGVLQAYEFAPIAMQVRQELDDNNIYTREW 73 + S+ GIP+A PP G RW+ P+ PAS E A + +D Sbjct: 61 VRSWLGIPYAQPPVGDARWQPPRALPASQAERTTTQPGAACVQTLSVPGMDKQ------- 113 Query: 74 AVEPDIAMSEDCLYLNVWTPAKRIDEKLPVYVWYFGGGLQVGHPAEMEFDGERIAR-RGI 132 + +EDCL+LNV+ P +K PV VW GG Q+G A ++D +AR +G+ Sbjct: 114 -----VRGAEDCLFLNVYAPTNA--QKAPVMVWIHGGSFQMG--AGSDYDLSVLAREQGV 164 Query: 133 VVVTINYRLNVFGFLCHPEITAEAPDAPANFGNLDQQAATRWVKRNIAAFGGDPDNITIG 192 V V++NYRL GFL P + A N G LDQQ A +WV+ NIAAFGGD N+T+ Sbjct: 165 VAVSLNYRLGALGFLATPALDTAQGTA-GNLGLLDQQLALKWVRDNIAAFGGDAQNVTVF 223 Query: 193 GQSAGGGSVMSQLTSPQNEGLFQRAIVESGIFTKLYPGTLMPALRSHLAEAEQDGIAFFN 252 G+SAGG S+ +QL SP GLF +AI++SG T P + AEA G F Sbjct: 224 GESAGGMSICAQLASPGAAGLFDKAIIQSGPCTA-------PGIMQSRAEAYNLGSRFAA 276 Query: 253 ELGVSSLAEARQLDAVYLRDKAV----EYGGFWGTVT-DQVF---SVGNSFDLFLQNKRW 304 +G A+A L AV DK V F G V V+ +V + L+ + Sbjct: 277 AVGCDP-ADAACLRAVPA-DKLVLTRSPNAAFPGAVPFPPVYGDSTVPRAPAEVLRQGQN 334 Query: 305 KVPVMFGHTSSE------FFSVPDAE-TFDDFKKMAVGMFGGDAAAFLELCGVRADNMEE 357 VP++ G +E F P + +F + + G +A L R Sbjct: 335 AVPLLIGTNLNEGTLFAAFVGDPRRDLNAAEFLGLNAVLNGPNAPRALLAYNSRTSGTRT 394 Query: 358 ARKRASVSGIEYAIRIAAQANAGTGGETPLYYYNFDAEIP----------GWDNPGTFHS 407 AS + +A + A TP+Y Y F P G + G FH Sbjct: 395 QAAAASATDSLFACPSSNLAR-DISRFTPVYSYEFRDPNPPRPAQLRPTVGVPSFGAFHG 453 Query: 408 VDLWFFFETLAKCWRP--FVGKHYDLARQMCNYWANFIRSGDPNGQDSTGEELPRWEPYT 465 ++ T + P F +L+R M YWANF R+G+PNG LP+W +T Sbjct: 454 SEIVSVTGTPSGLGNPSDFTPAQAELSRTMQTYWANFARTGNPNGAG-----LPQWAAFT 508 Query: 466 PEAP 469 E P Sbjct: 509 TEQP 512 >DR0415 [I] COG2272 Carboxylesterase type B Length = 506 Score = 157 bits (396), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 150/499 (30%), Positives = 200/499 (40%), Gaps = 69/499 (13%) Query: 1 VENGTVKGLPAADPRITSFKGIPFAAPPTGQNRWRAPQPASDWEGVLQAYEFAPIAMQVR 60 VE GT+ G A + F G+PFAAPP G RW+ PQP W G A +F P Q Sbjct: 25 VEQGTLNG--KAVNSVQQFLGVPFAAPPVGPLRWQPPQPPV-WSGQRDATQFGPACAQ-- 79 Query: 61 QELDDNNIYTREWAVEPDIAMSEDCLYLNVWTPAKRIDEKLPVYVWYFGGGLQVGHPAEM 120 D SEDCL LN++ P + P+ VW GG G + Sbjct: 80 ---GDGG--------------SEDCLTLNIYRPPN--AKNAPLLVWVHGGFFTGGDASL- 119 Query: 121 EFDGERIAR-RGIVVVTINYRLNVFGFLCHPEITAEAPDAPANFGNLDQQAATRWVKRNI 179 FDG +AR RG+VV T+NYRL ++GFL P +T N G D A RW+K+N Sbjct: 120 -FDGSALARERGVVVATVNYRLGLYGFLAVPGVTD------GNAGLYDILGAVRWLKQNA 172 Query: 180 AAFGGDPDNITIGGQSAGGGSVMSQLTSPQNEGLFQRAIVESGIFTKLYPGTLMPALRSH 239 A+ G DP NIT+ GQSAG ++ + L +P GLFQ+AI++SG P ++ Sbjct: 173 ASIGADPGNITLAGQSAGAAAICTLLAAPSAAGLFQKAILQSG-------SCAAPIFQTP 225 Query: 240 LAEAEQDGIAFFNELGVSSLAEAR--------QLDAVYLRDKAVEYGGFWGTVTDQVFSV 291 L +A + G F L A +LD V L + V Sbjct: 226 LGDARRKGELFSRALRCDRGETATCLRGKSRAELDGVRLTGRGPVDPVPLPPVYGDALIP 285 Query: 292 GNSFDLFLQNKRWKVPVMFGHTSSE--FFSVPDAETFDDFKKM-AVGMFGGDAAAFLELC 348 D F Q + KV VM G E F + + + G+ + L Sbjct: 286 LRPQDAFAQGQSLKVAVMLGINREEGQIFEPYLPDLLRGSRTLYRAGLTLINPLQAWALG 345 Query: 349 GVRADNMEEARKRASVSGIEYAIRIAAQA--NAGTGGETPLYYYNFDAEIPGWD------ 400 G +E RA + + + G P Y+Y F P Sbjct: 346 GRYPLRSDEQPIRAVTRLLTDQVFACPTSWLAGKLGAGLPTYFYEFSDPRPPLQLAGKVN 405 Query: 401 --NPGTFHSVDLWFFFET-LAKCWRP--FVGKHYDLARQMCNYWANFIRSGDPNGQDSTG 455 N G +H +L + +T + P F LA QM YW NF RSG P S G Sbjct: 406 VPNLGAYHGAELAYVLDTPVVGVSDPAQFSSAQAKLAAQMGEYWTNFARSGQPA---SLG 462 Query: 456 EELPRWEPYTPEAPYGMLF 474 L W + P P M Sbjct: 463 --LTVWPTFNPAFPQVMTL 479 >Rv2045c [I] COG2272 Carboxylesterase type B Length = 511 Score = 142 bits (357), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 86/208 (41%), Positives = 109/208 (52%), Gaps = 12/208 (5%) Query: 16 ITSFKGIPFAAPPTGQNRWRAPQPASDWEGVLQAYEFAPIAMQVRQ--ELDDNNIYTREW 73 + ++ IP+A P G R+RAPQPA W GV + FA A Q R+ + TR Sbjct: 33 VHRWRSIPYARAPVGSLRFRAPQPAQPWPGVRHCHTFANCAPQQRRYTVMGIGRYQTR-- 90 Query: 74 AVEPDIAMSEDCLYLNVWTPAKRIDEKLPVYVWYFGGGLQVGHPAEMEFDGERIARRGIV 133 SEDCL LNV TP + + LPV V+ GGG +G A +DG +ARRG V Sbjct: 91 --------SEDCLTLNVVTPEEPATQPLPVMVFIHGGGYILGSSATPIYDGAALARRGCV 142 Query: 134 VVTINYRLNVFGFLCHPEITAEAPDAPANFGNLDQQAATRWVKRNIAAFGGDPDNITIGG 193 V++NYRL G L ++ +N D A RWV NIA FGGDP N+TI G Sbjct: 143 YVSVNYRLGALGCLDLSSLSTPQITLDSNVYLRDLVLALRWVHDNIAEFGGDPGNVTIFG 202 Query: 194 QSAGGGSVMSQLTSPQNEGLFQRAIVES 221 +SAG + L P +GLF RAI ES Sbjct: 203 ESAGAHITATLLAVPAAKGLFARAISES 230 >Rv1104 [I] COG2272 Carboxylesterase type B Length = 229 Score = 116 bits (291), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 74/195 (37%), Positives = 98/195 (50%), Gaps = 13/195 (6%) Query: 4 GTVKGLPAADPRITSFKGIPFAAPPTGQNRWRAPQPASDWEGVLQAYEFAPIAMQVRQEL 63 G V+G A D R+ +KGI +AAPP G R+R P+P W V A F P Q Sbjct: 13 GPVRG--ADDGRVKVWKGIRYAAPPLGDLRFRTPEPPERWTEVADATTFGPACPQP---- 66 Query: 64 DDNNIYTREWAVEPDIAMSEDCLYLNVWTPA-KRIDEKLPVYVWYFGGGLQVGHPAEMEF 122 ++ + SEDC LN+W PA + PV VW GG +G ++ + Sbjct: 67 -----AIPNMPLDLGASQSEDCWSLNIWAPADTEPGDGKPVMVWLHGGAYILGSGSQPLY 121 Query: 123 DGERIARRG-IVVVTINYRLNVFGFLCHPEITAEAPDAPANFGNLDQQAATRWVKRNIAA 181 +G R+A G +VVVT+NYRL GFL +N G D A RWV NIA Sbjct: 122 NGRRLAASGDVVVVTVNYRLGALGFLDLSSFNTSRRRFDSNIGLRDVLAVLRWVADNIAV 181 Query: 182 FGGDPDNITIGGQSA 196 FGGDP+ +T+ G+SA Sbjct: 182 FGGDPEKVTLFGESA 196 >Cgl1109 [I] COG2272 Carboxylesterase type B Length = 481 Score = 111 bits (278), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 74/243 (30%), Positives = 116/243 (47%), Gaps = 11/243 (4%) Query: 79 IAMSEDCLYLNVWTPAKRIDEKLPVYVWYFGGGLQVGHPAEMEFDG-ERIARRGIVVVTI 137 I SEDCL L+V P +EKLPV V+ GG +G +E G + +V V++ Sbjct: 17 IRGSEDCLNLDVVRPDS--EEKLPVVVYLHGGSFIMGSSSEKALRGYNLVTNMNVVYVSV 74 Query: 138 NYRLNVFGFLCHPEITAEAPDAPANFGNLDQQAATRWVKRNIAAFGGDPDNITIGGQSAG 197 N+RL G+L ++ + D AN DQ A +WV RNI AFGGDPDN+T+ G+SAG Sbjct: 75 NFRLGALGYL---DLRSVGEDCVANPALHDQLLALQWVSRNIEAFGGDPDNVTLMGESAG 131 Query: 198 GGSVMSQLTSPQNEGLFQRAIVESGIFTKLYPGTLMP----ALRSHLAEAEQDGIAFFNE 253 +V++ + P GLF RAI +S ++ T L +A + + + Sbjct: 132 AAAVVALMCVPAAGGLFHRAIAQSAPVISVHSSTQAKFWARELIYRMALPRETTLDELRQ 191 Query: 254 LGVSSLAEARQLDAVYLRDKAVEYGGFWGTVTDQVFSVGNSFDLFLQNKRWKVPVMFGHT 313 L A Q ++ + ++ +G D + +F Q ++ ++P M G Sbjct: 192 ESADDLVRAGQ-SMMWRSGELLQLNSCYGPTVDGSLLPEHPLTMFEQGRQHRIPFMIGTN 250 Query: 314 SSE 316 + E Sbjct: 251 NGE 253 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.137 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 40,128,931 Number of extensions: 1732683 Number of successful extensions: 2520 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 2514 Number of HSP's successfully gapped: 11 Length of query: 501 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 390 Effective length of database: 25,576,357 Effective search space: 9974779230 Effective search space used: 9974779230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 118 (50.1 bits)