BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0417.0002.p (505 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value BS_ydfJ [R] COG2409 Predicted drug exporters of the RND superfamily 362 e-100 CAC3431 [R] COG2409 Predicted drug exporters of the RND superfamily 349 5e-96 Cgl0900 [R] COG2409 Predicted drug exporters of the RND superfamily 307 2e-83 Cgl0232 [R] COG2409 Predicted drug exporters of the RND superfamily 306 6e-83 ML2617 [R] COG2409 Predicted drug exporters of the RND superfamily 190 4e-48 >BS_ydfJ [R] COG2409 Predicted drug exporters of the RND superfamily Length = 427 Score = 362 bits (929), Expect = e-100, Method: Compositional matrix adjust. Identities = 0/494 (0%), Positives = 0/494 (0%), Gaps = 14/494 (2%) Query: 8 LAVMIGLAVGIDYSLFIVNRQRRLILDEKLSAREAASRAVGTAGSAVFFAGLTVIIALCG 67 + G V + + + L + + + + Sbjct: 233 QTFLAGYLVQTLLFNKAIPTDALM-ISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIV 291 Query: 68 MLVIGIGFLSTMXXXXXXXXXXXXXXXXXXXXXXXXXXGER-ICTAKARTKKAASGNNAH 126 + + + + + + Sbjct: 292 LAWPMLSIIVDKSYAPSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISK- 350 Query: 127 HGFSHRWANATVKYRWXXXXXXXXXXGTAAIPVTKMELGIPSGASANLDTPARQSYDIIS 186 + + T + + + S + D Sbjct: 351 ------EIGGLIASGFGPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDL 404 Query: 187 KGFGEGFNGPLLLVAEPKNPSGKISLETLGKLVQGLQMHDNVTLVSPMGVNATGDIAIIS 246 + +V + Sbjct: 405 SALEDAAEDQPRVVRAAQPSRSL- 463 Query: 247 LIPKTGPTDTETRELVQELRDPASSLASGNELTLGVTGFTAINIDMSSKLSDAFPXXXXX 306 Sbjct: 464 ---- 519 Query: 307 XXXXXXXXXXXXXXXXXXPIKATVGFILSVLATFGLTTAVYQWGWLHSLFGFDTGGPLLS 366 Sbjct: 520 579 Query: 367 FMPILVTGILYGLAMDYQVFLVSSMREAYVHGRHGKESVVHGYDLASRVVLAAGIIMVSV 426 Sbjct: 580 639 Query: 427 FAGFIFAPDAMIKQIGFALAFGILIDAFIIRMTLVPAVMAVFGDKAWWLPKWLDRLLPNL 486 Sbjct: 640 699 Query: 487 DVEGDKLIAKLHAE 500 Sbjct: 700 713 >CAC3431 [R] COG2409 Predicted drug exporters of the RND superfamily Length = 729 Score = 349 bits (896), Expect = 5e-96, Method: Compositional matrix adjust. Identities = 194/495 (39%), Positives = 281/495 (56%), Gaps = 7/495 (1%) Query: 8 LAVMIGLAVGIDYSLFIVNRQRRLILDEKLSAREAASRAVGTAGSAVFFAGLTVIIALCG 67 LA MIGLAVGIDY+LFI++R R L E +E+ +RA+ TAGS+V FAG+TVIIAL G Sbjct: 238 LAAMIGLAVGIDYALFIISRYREN-LAEGYDRQESLARAMATAGSSVLFAGVTVIIALSG 296 Query: 68 MLVIGIGFLSTMXXXXXXXXXXXXXXXXXXXXXXXXXXGERICT-AKARTKKAASGNNAH 126 + ++G+ FL TM +RI K + + Sbjct: 297 LSLVGVPFLRTMGLDAAAVVLLAIISSLTAVPAFISIAKDRISIYKKNKIFNIIKSSKYK 356 Query: 127 HGFSHRWANATVKYRWXXXXXXXXXXGTAAIPVTKMELGIPSGASANLDTPARQSYDIIS 186 + +W + K+ + +ELGIP S +++ R+ YDI++ Sbjct: 357 NKEDSKWGSIVSKHPAVIAIAVVIVTALFSYQALNLELGIPDKGSKPIESTERKGYDIMA 416 Query: 187 KGFGEGFNGPLLLVAEPKNPSGK--ISLETLGKLVQGLQMHDNVTLVSPMGVNATGDIAI 244 +GFG+G NGPL+LVA+ G I+L+ + K + L ++T P+G N +A+ Sbjct: 417 EGFGKGVNGPLILVADASKVKGNYMITLQEIEKEIGKLPNIKSITPPIPVGNN---KLAM 473 Query: 245 ISLIPKTGPTDTETRELVQELRDPASSLASGNELTLGVTGFTAINIDMSSKLSDAFPXXX 304 I+L+PK+GP D ET+ELV+ + + + ++ ++ L TG TA+NID LS P Sbjct: 474 INLVPKSGPNDKETKELVKAINEKSENIRKYKKVDLMETGATAVNIDTDVTLSKVMPKFI 533 Query: 305 XXXXXXXXXXXXXXXXXXXXPIKATVGFILSVLATFGLTTAVYQWGWLHSLFGFDTGGPL 364 PIKA +GF++++L+T G V Q G L LF P+ Sbjct: 534 LVIVGLAIILMVLVFRSILVPIKAVLGFLMTILSTLGFGVIVLQNGHLAGLFNVAAREPI 593 Query: 365 LSFMPILVTGILYGLAMDYQVFLVSSMREAYVHGRHGKESVVHGYDLASRVVLAAGIIMV 424 L F+PILVTGIL+GLAMDY+VFLVS MRE+Y ++SV+ G + +VV +A +IM Sbjct: 594 LCFLPILVTGILFGLAMDYEVFLVSRMRESYTQTGDARKSVILGIKNSGKVVTSAALIMT 653 Query: 425 SVFAGFIFAPDAMIKQIGFALAFGILIDAFIIRMTLVPAVMAVFGDKAWWLPKWLDRLLP 484 +VF GF F DA +K++G LAFG+L DAF+IRMT VPAVM + G KAW++PKWLDR+LP Sbjct: 654 AVFLGFAFNVDANVKEMGITLAFGVLFDAFVIRMTFVPAVMILLGKKAWYMPKWLDRILP 713 Query: 485 NLDVEGDKLIAKLHA 499 N+D+EG+ +I + A Sbjct: 714 NIDIEGESIIKEEKA 728 >Cgl0900 [R] COG2409 Predicted drug exporters of the RND superfamily Length = 736 Score = 307 bits (787), Expect = 2e-83, Method: Compositional matrix adjust. Identities = 191/518 (36%), Positives = 261/518 (50%), Gaps = 25/518 (4%) Query: 1 MNDITPILAVMIGLAVGIDYSLFIVNRQRRLILDE----KLSAREAASR----------- 45 +ND+TP LA MIGLAVGIDY+LFIV+R R ++ + L +E A R Sbjct: 209 VNDMTPTLASMIGLAVGIDYALFIVSRFRNELISQTGANDLEPKELAERLRTMPLAARAH 268 Query: 46 ----AVGTAGSAVFFAGLTVIIALCGMLVIGIGFLSTMXXXXXXXXXXXXXXXXXXXXXX 101 AVGTAGSAV FAG TV+IAL + +I I FL+ M Sbjct: 269 AMGMAVGTAGSAVVFAGTTVLIALVALSIINIPFLTVMAIAAAITVAIAVLVALSFLPAL 328 Query: 102 XXXXGERICTAKARTKKAASGNNAHHGFSHRWANATVKYRWXXXXXXXXXXGTAAIPVTK 161 G RI A+ K + +W K G AIP T Sbjct: 329 LGLLGTRIFAARVPGPKVPDPEDEKPTMGLKWVRLVRKMPVAYLLVGVVLLGAIAIPATN 388 Query: 162 MELGIPSGASANLDTPARQSYDIISKGFGEGFNGPLLLVAEPKNPSGKISLETLGKLVQG 221 M L +P+ ++ L T R +YD+ + FG G N P++ + + + + G+ V+ Sbjct: 389 MRLAMPTDGTSTLGTAPRTAYDMTADAFGPGRNAPMIALIDATDVPEEERPLVFGQAVEQ 448 Query: 222 LQMHDNVTLVSPMGVNATGDIAIISLIPKTGPTDTETRELVQELRDPASSLASGNELTLG 281 D V D A I + P+ D T E + LR A + A T G Sbjct: 449 FLNTDGVKNAQITQTTENFDTAQILITPEFDAIDERTSETLATLRADAETFADDTGATYG 508 Query: 282 VTGFTAINIDMSSKLSDAFPXXXXXXXXXXXXXXXXXXXXXXXPIKATVGFILSVLATFG 341 +TG T I D+S++L D P+ A +GF LSVLATFG Sbjct: 509 ITGVTPIYDDISARLGDVLVPYVLIVLVLAFLVLLLVFRSIWVPLIAALGFGLSVLATFG 568 Query: 342 LTTAVYQWGWLHSLFGF-DTGGPLLSFMPILVTGILYGLAMDYQVFLVSSMREAYVHGRH 400 T A++Q G FG D PLLSF+PI++ G+++GLAMDYQ+FLV+ MRE + G+ Sbjct: 569 ATVAIFQEGA----FGIIDDPQPLLSFLPIMLIGLVFGLAMDYQIFLVTRMREGFTKGKT 624 Query: 401 GKESVVHGYDLASRVVLAAGIIMVSVFAGFIFAPDAMIKQIGFALAFGILIDAFIIRMTL 460 + +G+ +RVV AA +IMVSVFA FI A IK +GFALA + DAF++RM + Sbjct: 625 AGNATSNGFKHGARVVTAAALIMVSVFAAFIAQDMAFIKTMGFALAVAVFFDAFVVRMMI 684 Query: 461 VPAVMAVFGDKAWWLPKWLDRLLPNLDVEGDKLIAKLH 498 +PA M + DKAWWLPKWLD++LPN+DVEG+ L ++LH Sbjct: 685 IPATMFLLDDKAWWLPKWLDKILPNVDVEGEGL-SELH 721 >Cgl0232 [R] COG2409 Predicted drug exporters of the RND superfamily Length = 801 Score = 306 bits (784), Expect = 6e-83, Method: Compositional matrix adjust. Identities = 198/534 (37%), Positives = 268/534 (50%), Gaps = 47/534 (8%) Query: 1 MNDITPILAVMIGLAVGIDYSLFIVNRQRRLILDEKLSAREAASRAVGTAGSAVFFAGLT 60 +N++TP+LAVMIGLAVGIDY+LFI++R R +++ +AA AVGTAGSAV FAG T Sbjct: 263 LNNVTPVLAVMIGLAVGIDYALFILSRYRAEY--KRMPRADAAGMAVGTAGSAVVFAGAT 320 Query: 61 VIIALCGMLVIGIGFLSTMXXXXXXXXXXXXXXXXXXXXXXXXXXGERICTAK------- 113 VIIAL +++ IGFL+ M G K Sbjct: 321 VIIALVALIIADIGFLTAMGISAAFTVFVAVLIALTFIPALLGVFGGHAFKGKIPGIGGN 380 Query: 114 ARTKKAASGNNAHHGFSHRWANATVKYRWXXXXXXXXXXGTAAIPVTKMELGIPSGASAN 173 K+ W K G IP ++L +PS +++N Sbjct: 381 PTPKQTWEQALNRRSKGRSWVKLVQKAPGLVVAVVVLGLGALTIPAMNLQLSLPSDSTSN 440 Query: 174 LDTPARQSYDIISKGFGEGFNGPLLLVA----------------EPKNPS-GKISLETLG 216 +DT RQS D++++GFG G N P L++ E + P G+ E Sbjct: 441 IDTTQRQSADLMAEGFGAGVNAPFLVIVDTHEVNADSTALQPLIEAQEPEEGEFDREQAA 500 Query: 217 K------LVQGLQMHDNVTLVSPMGVNATGDIAIISLIPKTGPTDTETRELVQELRDPAS 270 + + Q + +V + VN A I + P TGP D ET EL+ LR + Sbjct: 501 RFATYMYVTQTYNSNIDVKNAQIISVNDDFTAAQILVTPYTGPADKETPELMHVLRAQEA 560 Query: 271 SLASGNELTLGVTGFTAINIDMSSKLSDAFPXXXXXXXXXXXXXXXXXXXXXXXPIKATV 330 + LG TGFTA+ +D++ +L DA P P+ A + Sbjct: 561 QIEDVTGTELGTTGFTAVQLDITEQLEDAMPVYLAVVVGLAIFLLILVFRSLLVPLVAGL 620 Query: 331 GFILSVLATFGLTTAVYQWGWLHSLFGF-DTGGPLLSFMPILVTGILYGLAMDYQVFLVS 389 GF+LSV A FG T V+Q G+ GF +T GPL+SFMPI + G+ +GLAMDYQVFLV+ Sbjct: 621 GFLLSVGAAFGATVLVWQEGF----GGFVNTPGPLISFMPIFLIGVTFGLAMDYQVFLVT 676 Query: 390 SMREAYVH----GRHG------KESVVHGYDLASRVVLAAGIIMVSVFAGFIFAPDAMIK 439 MRE Y H G+ G ++SV+ G+ SRVV AA +IM++VF FI P IK Sbjct: 677 RMREHYTHHNGKGQPGSKYTPVEQSVIEGFTQGSRVVTAAALIMIAVFVAFIDQPLPFIK 736 Query: 440 QIGFALAFGILIDAFIIRMTLVPAVMAVFGDKAWWLPKWLDRLLPNLDVEGDKL 493 GFAL G+ DAF IRM LVPA M + G WW+PKWLDR+LP+LD+EG L Sbjct: 737 IFGFALGAGVFFDAFFIRMGLVPASMFLMGKATWWMPKWLDRILPSLDIEGTAL 790 >ML2617 [R] COG2409 Predicted drug exporters of the RND superfamily Length = 311 Score = 190 bits (483), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 0/484 (0%), Positives = 0/484 (0%), Gaps = 9/484 (1%) Query: 11 MIGLAVGIDYSLFIVNRQRRLILDEKLSAREAASRAVGTAGSAVFFAGLTVIIALCGMLV 70 +G IV RL + + + A A + ++ + ++ Sbjct: 239 AWVGLLGSIIPGLIVAWAYRLAFVSL-TPSLQSQKLAMFAPKEFIPAIVGLMAVIGILIG 297 Query: 71 IGIGFLSTMXXXXXXXXXXXXXXXXXXXXXXXXXXGERICTAKARTKKAASGNNAHHGFS 130 Sbjct: 298 357 Query: 131 HRWANATVKYRWXXXXXXXXXXGTAAIPVTKMELGIPSGASANLDTPARQSYDIISKGFG 190 Sbjct: 358 417 Query: 191 EGFNGPL-LLVAEPKNPSGKIS----LETLGKLVQGLQMHDNVTLVSPMGVNATGDIAII 245 Sbjct: 418 477 Query: 246 SLIPKTGPTDTETRELVQELRDPASSLASGNELTLGVTGFTAINIDMSSKLSDAFPXXXX 305 Sbjct: 478 -- 535 Query: 306 XXXXXXXXXXXXXXXXXXXPIKATVGFILSVLATFGLTTAVYQWGWLHSLFGFDTGGPLL 365 Sbjct: 536 - 594 Query: 366 SFMPILVTGILYGLAMDYQVFLVSSMREAYVHGRHGKESVVHGYDLASRVVLAAGIIMVS 425 Sbjct: 595 654 Query: 426 VFAGFIFAPDAMIKQIGFALAFGILIDAFIIRMTLVPAVMAVFGDKAWWLPKWLDRLLPN 485 Sbjct: 655 714 Query: 486 LDVE 489 Sbjct: 715 718 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.138 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 32,497,100 Number of extensions: 1211350 Number of successful extensions: 3012 Number of sequences better than 1.0e-05: 26 Number of HSP's gapped: 2975 Number of HSP's successfully gapped: 35 Length of query: 505 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 394 Effective length of database: 25,576,357 Effective search space: 10077084658 Effective search space used: 10077084658 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 118 (50.1 bits)