BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0416.0004.p (250 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value RSc0729 [E] COG0112 Glycine/serine hydroxymethyltransferase 277 1e-74 RSp0055 [E] COG0112 Glycine/serine hydroxymethyltransferase 276 3e-74 mlr8400 [E] COG0112 Glycine/serine hydroxymethyltransferase 273 2e-73 VCA0278 [E] COG0112 Glycine/serine hydroxymethyltransferase 269 3e-72 AGc2156 [E] COG0112 Glycine/serine hydroxymethyltransferase 268 4e-72 >RSc0729 [E] COG0112 Glycine/serine hydroxymethyltransferase Length = 415 Score = 277 bits (709), Expect = 1e-74, Method: Compositional matrix adjust. Identities = 132/245 (53%), Positives = 173/245 (70%) Query: 1 MIVAGASAYPRTIEFEPFAQIAAEVGALFFVDMAHIAGIVAAGFHPSPIPHAHFVTTTTH 60 +I+AGASA+ I+FE A++A VGA F VDMAH AG++AAG +P+P+PHA FVTTTTH Sbjct: 170 LIIAGASAFALRIDFERIAKVAKAVGAYFMVDMAHYAGLIAAGVYPNPVPHADFVTTTTH 229 Query: 61 KTLRGPRGGIIMCRKPWAQAMDKAIFPGVQGGPLMHAIAAKTVAFGEALQPEFKSYIEKV 120 K+LRGPRGG+I+ + +A++ AIFPG+QGGPLMH IA K VAF EA P FK+Y E+V Sbjct: 230 KSLRGPRGGVILMKAEHEKAINSAIFPGIQGGPLMHVIAGKAVAFKEAQSPTFKAYQEQV 289 Query: 121 LVNAKVLAEALLNEGLTVVSGGTDNHITLLDLRPIGLNGKEAESILDEVGITANKNAIPH 180 + NA+ +AE L+ GL +VSG T++H+ L+DLR + GKEAE +L + IT NKNAIP+ Sbjct: 290 VKNARAMAETLMARGLRIVSGRTESHVMLVDLRAKSITGKEAEKVLGDAHITVNKNAIPN 349 Query: 181 DTASPLITSGIRFGTPAMTTRGMGPQEMKEIARLIGLALKKPESSVAKEQLRRSVQEITS 240 D P +TSGIR G+PAMTTRG E ++A LI L P +R V E+T Sbjct: 350 DPEKPFVTSGIRLGSPAMTTRGFKEGEAVKVAHLIADVLDNPHDEANIAAVRAKVAELTK 409 Query: 241 QFPLY 245 QFP+Y Sbjct: 410 QFPVY 414 >RSp0055 [E] COG0112 Glycine/serine hydroxymethyltransferase Length = 424 Score = 276 bits (705), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 137/251 (54%), Positives = 174/251 (69%), Gaps = 6/251 (2%) Query: 1 MIVAGASAYPRTIEFEPFAQIAAEVGALFFVDMAHIAGIVAAGFHPSPIPHAHFVTTTTH 60 +I+AG SAYPR ++F F IA VGA VDMAHIAG++AAG H +P+ HAH VT+TTH Sbjct: 174 LIIAGFSAYPRKLDFARFRAIADSVGAKLMVDMAHIAGVIAAGRHDNPVDHAHVVTSTTH 233 Query: 61 KTLRGPRGGIIMCR-KPWAQAMDKAIFPGVQGGPLMHAIAAKTVAFGEALQPEFKSYIEK 119 KTLRGPRGG ++ + A+ ++ A+FPG+QGGPLMH IA K VAFGEAL+PEFK+YI+ Sbjct: 234 KTLRGPRGGFVLTNDEEIAKKINSAVFPGLQGGPLMHVIAGKAVAFGEALKPEFKTYIDS 293 Query: 120 VLVNAKVLAEALLNEGLTVVSGGTDNHITLLDLRPIGLNGKEAESILDEVGITANKNAIP 179 VL NAK L E L G+ +V+GGTDNH+ L+DLRP GL G + E L+ GIT NKN IP Sbjct: 294 VLANAKALGEVLKAGGVDLVTGGTDNHLLLVDLRPKGLKGTQVEQALERAGITCNKNGIP 353 Query: 180 HDTASPLITSGIRFGTPAMTTRGMGPQEMKEIARLIGLALK----KPESSVAKE-QLRRS 234 DT P ITSGIR GTPA TTRG G E ++I RLI + P+ A E ++R Sbjct: 354 FDTEKPTITSGIRLGTPAATTRGFGVAEFEQIGRLILEVFEALRANPDGDRATEHRVRSE 413 Query: 235 VQEITSQFPLY 245 + + +FP+Y Sbjct: 414 IFALCDRFPIY 424 >mlr8400 [E] COG0112 Glycine/serine hydroxymethyltransferase Length = 301 Score = 273 bits (698), Expect = 2e-73, Method: Compositional matrix adjust. Identities = 0/253 (0%), Positives = 0/253 (0%), Gaps = 8/253 (3%) Query: 1 MIVAGASAYPRTIEFEPFAQIAAEVGALFFVDMAHIAGIVAAGFHPSPIPHAHFVTTTTH 60 R + E + + + Sbjct: 181 PEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEIERFVSNERGGYDINGLILVERE 240 Query: 61 KTLRGPRGGIIMCR-KPWAQAMDKAIFPGVQGGPLMHAIAAKTVAFGEALQPEFKSYIEK 119 + G +A L + K+ + Y++ Sbjct: 241 GQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALRSLGYVDD 300 Query: 120 VLVNAKVLAEALLNEGLTVVSGGTDNHITLLDLRPIGLNGKEAESILDEVGITANKNAIP 179 Sbjct: 301 360 Query: 180 HDTASPLITSGIRFGTPAMTTRGMGPQEMKEIARLIGLALKKPESSVAKE-------QLR 232 Sbjct: 361 420 Query: 233 RSVQEITSQFPLY 245 Sbjct: 421 433 >VCA0278 [E] COG0112 Glycine/serine hydroxymethyltransferase Length = 435 Score = 269 bits (688), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 145/252 (57%), Positives = 179/252 (71%), Gaps = 6/252 (2%) Query: 1 MIVAGASAYPRTIEFEPFAQIAAEVGALFFVDMAHIAGIVAAGFHPSPIPHAHFVTTTTH 60 MI+AG SA PRTI+F F I EVGAL VDMAHIAG+VA G HPSP+PHAH VTTTTH Sbjct: 184 MIIAGGSAIPRTIDFAQFRSIVDEVGALLMVDMAHIAGLVATGAHPSPLPHAHVVTTTTH 243 Query: 61 KTLRGPRGGIIMCR-KPWAQAMDKAIFPGVQGGPLMHAIAAKTVAFGEALQPEFKSYIEK 119 KTLRGPRGG+I+ + + ++ A+FPG+QGGPLMH IAAK VAFGEAL PEF++YI+ Sbjct: 244 KTLRGPRGGMILTNSEEIHKKINSAVFPGLQGGPLMHVIAAKAVAFGEALGPEFRTYIDS 303 Query: 120 VLVNAKVLAEALLNEGLTVVSGGTDNHITLLDLRPIGLNGKEAESILDEVGITANKNAIP 179 V+ NAKVLAE L G +V+GGTD H+ L+DLRP GL G + E L+ GIT NKN IP Sbjct: 304 VIDNAKVLAEVLQTRGCDIVTGGTDTHLMLVDLRPKGLKGNQVEQALERAGITCNKNGIP 363 Query: 180 HDTASPLITSGIRFGTPAMTTRGMGPQEMKEIARLIGLALK----KPESS-VAKEQLRRS 234 D P+ITSGIR GTPA T+RG G +E K I IG L PE + ++Q+R+ Sbjct: 364 FDEEKPMITSGIRLGTPAGTSRGFGREEFKLIGEWIGDVLDGLVASPEGNPDVEQQVRKQ 423 Query: 235 VQEITSQFPLYE 246 V+ + +FPLY+ Sbjct: 424 VKALCQRFPLYQ 435 >AGc2156 [E] COG0112 Glycine/serine hydroxymethyltransferase Length = 301 Score = 268 bits (686), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 0/253 (0%), Positives = 0/253 (0%), Gaps = 8/253 (3%) Query: 1 MIVAGASAYPRTIEFEPFAQIAAEVGALFFVDMAHIAGIVAAGFHPSPIPHAHFVTTTTH 60 ++ + +M + + Sbjct: 236 LVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLELWVKVKSGWADDERALRSL 295 Query: 61 KTLRGPRGGIIMCR-KPWAQAMDKAIFPGVQGGPLMHAIAAKTVAFGEALQPEFKSYIEK 119 Sbjct: 296 355 Query: 120 VLVNAKVLAEALLNEGLTVVSGGTDNHITLLDLRPIGLNGKEAESILDEVGITANKNAIP 179 Sbjct: 356 415 Query: 180 HDTASPLITSGIRFGTPAMTTRGMGPQEMKEIARLI-----GLALKKPESSVAKEQ--LR 232 Sbjct: 416 475 Query: 233 RSVQEITSQFPLY 245 Sbjct: 476 488 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 18,622,932 Number of extensions: 839776 Number of successful extensions: 1584 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 1538 Number of HSP's successfully gapped: 29 Length of query: 250 Length of database: 38,378,431 Length adjustment: 104 Effective length of query: 146 Effective length of database: 26,383,695 Effective search space: 3852019470 Effective search space used: 3852019470 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 114 (48.5 bits)