BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0412.0014.p (223 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1195 [IQR] COG1028 Dehydrogenases with different specificitie... 120 1e-27 TM1195 [G] COG1874 Beta-galactosidase 120 1e-27 TM0310 [G] COG1874 Beta-galactosidase 110 2e-24 STM0310 [G] COG0279 Phosphoheptose isomerase 110 2e-24 BH3701 [G] COG1874 Beta-galactosidase 103 2e-22 >STM1195 [IQR] COG1028 Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases) Length = 244 Score = 120 bits (302), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 10/177 (5%), Positives = 22/177 (12%), Gaps = 5/177 (2%) Query: 7 GVAYYDEYMPYERLDEDIAMMKEAGINTVRIAESTWSTHEPQNGVFDFTSVDRVLDAMHA 66 R I +T + + ++ + Sbjct: 3 FEGKIALVTGASRGIGRAIAETLVARGAKVIGTATSENGAKNISDYLGANGKGLMLNVTD 62 Query: 67 AGIAVIVGTPTYAVPAWLVKEHPDVLAVTPKGPGK-YGARQIMDITNPAYLFHAERIIRK 125 V A + + + I Sbjct: 63 PASIESVLENIRAEFGEVDILVNNAGITRDNLLMRMKDDEWNDIIETNLSSVFRLSKAVM 122 Query: 126 LISRVCKHPAVIGYQTDNETKHYDTA---GENVQLLFVKYMRETF-GTLEEINRRFG 178 + +I + T L I Sbjct: 123 RAMMKKRCGRIITIGSVVGTMGNAGQANYAAAKAGLIGFSKSLAREVASRGITVNVV 179 >TM1195 [G] COG1874 Beta-galactosidase Length = 649 Score = 120 bits (302), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 64/177 (36%), Positives = 101/177 (57%), Gaps = 5/177 (2%) Query: 7 GVAYYDEYMPYERLDEDIAMMKEAGINTVRIAESTWSTHEPQNGVFDFTSVDRVLDAMHA 66 GV YY E+ E+++ED MKE GI VRI E WS E + G F++ +D+ L+ Sbjct: 3 GVCYYPEHWGTEKVEEDFRRMKELGIEYVRIGEFAWSRIESERGKFNWDWLDKTLELAEK 62 Query: 67 AGIAVIVGTPTYAVPAWLVKEHPDVLAVTPKGPGK-YGARQIMDITNPAYLFHAERIIRK 125 G+ +++GTPT P WL+ EHP++L V G K +G+R+ ++P Y +RI+ Sbjct: 63 MGLKIVLGTPTATPPKWLIDEHPEILPVDKDGRVKNFGSRRHYCFSSPVYREEVKRIVTI 122 Query: 126 LISRVCKHPAVIGYQTDNETKHYDTA---GENVQLLFVKYMRETF-GTLEEINRRFG 178 ++ R KHPAV G+QTDNE +DT + F K++ + G ++++N +G Sbjct: 123 IVKRYGKHPAVAGWQTDNEYGCHDTVRCYCPRCKKAFQKWLERKYEGDIKKLNEAWG 179 >TM0310 [G] COG1874 Beta-galactosidase Length = 672 Score = 110 bits (275), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 59/179 (32%), Positives = 95/179 (53%), Gaps = 2/179 (1%) Query: 1 MSTLRYGVAYYDEYMPYERLDEDIAMMKEAGINTVRIAESTWSTHEPQNGVFDFTSVDRV 60 + + YG YY E E + DI M KEAGIN V I +WS +P +DF+ ++V Sbjct: 6 LPVIWYGGDYYPEQWDEETFERDIRMFKEAGINVVTIGVFSWSLIQPDENTYDFSFFEKV 65 Query: 61 LDAMHAAGIAVIVGTPTYAVPAWLVKEHPDVLAVTPKGPGK-YGARQIMDITNPAYLFHA 119 +D ++ GI V + TPT A P W+ +++P++L G + G RQ + Y + A Sbjct: 66 MDRLYKEGIYVCLATPTSAPPHWMTQKYPEILFTDVNGVKREKGGRQNFCPNSEKYRYFA 125 Query: 120 ERIIRKLISRVCKHPAVIGYQTDNETKHYDTAGENVQLLFVKYMRETFGTLEEINRRFG 178 I KL HPA++ + +NE +Y + + F +++E +GTL+E+NRR+ Sbjct: 126 RNIAEKLAEHFKDHPALVLWHVNNEYLNY-CYCDICRGKFQNWLKEKYGTLDELNRRWN 183 >STM0310 [G] COG0279 Phosphoheptose isomerase Length = 192 Score = 110 bits (275), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 12/179 (6%), Positives = 29/179 (16%), Gaps = 2/179 (1%) Query: 1 MSTLRYGVAYYDEYMPYERLDEDIAMMKEAGINTVRIAESTWSTHEPQNGVFDFTSVDRV 60 + A + + + A + D Sbjct: 6 IRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAGGKVLSCGNGGSHCDAMHFAEE 65 Query: 61 LDAMHAAGIAVIVGTPTYAVPAWLVKEHPDVLAVTPKGPGK-YGARQIMDITNPAYLFHA 119 L + V + + G + + Sbjct: 66 LTGRYRENRPGYPAIAISDVSHISCVSNDFGYDYIFSRYVEAVGREGDVLLGISTSGNSG 125 Query: 120 ERIIRKLISRVCKHPAVIGYQTDNETKHYDTAGENVQLLFVKYMRETFGTLEEINRRFG 178 I +R + D TA +++ Y ++ Sbjct: 126 NVIKAIAAAREKGMKVITLTGKDGGKMAG-TADIEIRVPHFGYADRIQEIHIKVIHILI 183 >BH3701 [G] COG1874 Beta-galactosidase Length = 672 Score = 103 bits (257), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 58/171 (33%), Positives = 91/171 (53%), Gaps = 2/171 (1%) Query: 6 YGVAYYDEYMPYERLDEDIAMMKEAGINTVRIAESTWSTHEPQNGVFDFTSVDRVLDAMH 65 YG Y E E DEDI M K AGI+ + +W+ ++P +DFT +D +D ++ Sbjct: 11 YGGDYNPEQWEKEVWDEDIRMFKLAGIDVATLNVFSWALNQPDEETYDFTWLDEQIDRLY 70 Query: 66 AAGIAVIVGTPTYAVPAWLVKEHPDVLAVTPKGPGK-YGARQIMDITNPAYLFHAERIIR 124 GI + T T A PAW+ K++PDVL V +G + +G R +P Y +AER+ Sbjct: 71 ENGIYTCLATSTAAHPAWMAKKYPDVLRVDYQGRKRAFGGRHNSCPNSPTYRKYAERMAD 130 Query: 125 KLISRVCKHPAVIGYQTDNETKHYDTAGENVQLLFVKYMRETFGTLEEINR 175 +L R HP V+ + NE Y +N F K++++ +GTL+ +N+ Sbjct: 131 RLGERYKDHPGVLIWHVSNEYGGY-CYCDNCAASFRKWLQQKYGTLQNVNK 180 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.137 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,842,636 Number of extensions: 656179 Number of successful extensions: 2453 Number of sequences better than 1.0e-05: 8 Number of HSP's gapped: 2443 Number of HSP's successfully gapped: 8 Length of query: 223 Length of database: 38,378,431 Length adjustment: 102 Effective length of query: 121 Effective length of database: 26,614,363 Effective search space: 3220337923 Effective search space used: 3220337923 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 114 (48.5 bits)