BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0412.0012.p (296 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SP1896 [G] COG1175 ABC-type sugar transport systems, permease co... 174 2e-43 Cgl2407 [G] COG1175 ABC-type sugar transport systems, permease c... 170 3e-42 BS_yurN [G] COG1175 ABC-type sugar transport systems, permease c... 169 4e-42 PH1215 [G] COG1175 ABC-type sugar transport systems, permease co... 155 7e-38 YPO1722 [G] COG1175 ABC-type sugar transport systems, permease c... 148 1e-35 >SP1896 [G] COG1175 ABC-type sugar transport systems, permease components Length = 296 Score = 174 bits (440), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 101/281 (35%), Positives = 159/281 (56%), Gaps = 4/281 (1%) Query: 14 AFVAPAFIIYLLFLLIPTIGGMYYSFTDWNGLNPNYSFTGLGNFIEALREDPDFVNSLLF 73 AFV ++ +F +P G +YSFT+W GL NY F GL NF + L DP F+N++ F Sbjct: 20 AFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLTYNYKFVGLNNF-KLLFMDPKFMNAIGF 78 Query: 74 TLKYVLFMIVLQNVFALVLAVFIETKTRTKGFFRTIFFMPNMISTIIGAFMWTFVFSQVL 133 T + M+V + + +A + +K + + FFR FF P ++S + A ++ VF+ L Sbjct: 79 TAIIAIAMVVGEIALGIFIARVLNSKIKGQTFFRAWFFFPAVLSGLTVALIFKQVFNYGL 138 Query: 134 PQIAEKTAMSFLDQLWIGDPKVSFYSIIIVSLWHGVGYMMIIYLAALQGVPQSLKEAAVI 193 P I + F +G + ++ + V LW GV +II+LA LQ +P + EAA I Sbjct: 139 PAIGNALHIEFFQTSLLGTKWGAIFAAVFVLLWQGVAMPIIIFLAGLQSIPTEITEAARI 198 Query: 194 DGASPFQTFRKVTLPMITHAVTICFFLTLNGTFKVFEAVYGLTGGGPGRSTQVITMNIYE 253 DGA+ Q F + LP + +V++ F L L G F+ V+ +TGGGP +T + + +Y Sbjct: 199 DGATSKQVFWNIELPYLLPSVSMVFILALKGGLTAFDQVFAMTGGGPNNATTSLGLLVYN 258 Query: 254 EAFSNNFRYGYASAKSVILFVIILXXXXTFIQIGVMKRKEV 294 AF NN ++GYA+A +VILF +I+ + IQ+ V K+ E+ Sbjct: 259 YAFKNN-QFGYANAIAVILFFLIV--VISIIQLRVSKKFEI 296 >Cgl2407 [G] COG1175 ABC-type sugar transport systems, permease components Length = 281 Score = 170 bits (430), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 96/282 (34%), Positives = 158/282 (56%), Gaps = 12/282 (4%) Query: 15 FVAPAFIIYLLFLLIPTIGGMYYSFTDWNGLNPNYSFTGLGNFIEALREDPDFVNSLLFT 74 FV P + +++ L+P I G + SFT + + N + G+ N+++A + F+++ FT Sbjct: 12 FVLPTLLAFMIAFLVPFIVGFFLSFTKFTTIT-NAKWVGIDNYVKAFSQREGFISAFGFT 70 Query: 75 LKYVLFMIVLQNVFALVLAVFIETKTRTKGFFRTIFFMPNMISTIIGAFMWTFVFSQVLP 134 + V+ ++ N+FA +LA + K R FFRT+FFMPN+I I+ + W + + VL Sbjct: 71 VLVVIVSVITVNIFAFLLAWLLTRKLRGTNFFRTVFFMPNLIGGIVLGYTWQTMINAVLS 130 Query: 135 QIAEKTAMSFLDQLWIGDPKVSFYSIIIVSLWHGVGYMMIIYLAALQGVPQSLKEAAVID 194 A + D K + +I++ W +GYMMIIY+A LQ VP L EAA +D Sbjct: 131 HYATTIS---------ADWKFGYAGLIMLLNWQLIGYMMIIYIAGLQNVPPELIEAAELD 181 Query: 195 GASPFQTFRKVTLPMITHAVTICFFLTLNGTFKVFEAVYGLTGGGPGRSTQVITMNIYEE 254 G + ++ R VT+PM+ ++TIC FLTL+ +FK+F+ LT G PG T+++ +NI Sbjct: 182 GVNKWEMLRHVTIPMVMPSITICLFLTLSNSFKLFDQNLALTNGAPGGQTEMVALNIINT 241 Query: 255 AFSNNFRYGYASAKSVILFVIILXXXXTFIQIGVMKRKEVEA 296 F+ G AK+VI +++ + Q+ + KE+EA Sbjct: 242 LFNRMNVEGVGQAKAVI--FVVVVVVIAYFQLRATRSKEIEA 281 >BS_yurN [G] COG1175 ABC-type sugar transport systems, permease components Length = 292 Score = 169 bits (429), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 95/268 (35%), Positives = 155/268 (57%), Gaps = 8/268 (2%) Query: 15 FVAPAFIIYLLFLLIPTIGGMYYSFTDWNGLNPNYSFTGLGNFIEALREDPDFVNSLLFT 74 F+ PA +++LLF+ IP ++ S W+ +P +F GL N++E L DP F +L Sbjct: 12 FLVPA-LVFLLFVYIPIFENVFLSLFQWSSFSPEKTFIGLKNYVE-LFHDPVFYQALTNN 69 Query: 75 LKYVLFMIVLQNVFALVLAVFIETKTRTK--GFFRTIFFMPNMISTIIGAFMWTFVFSQ- 131 + Y + IV Q L+LA +E K K FFRT+FF+P +IS + A ++ F+++ Sbjct: 70 VLYAVISIVCQVFGGLILAAVLEDKLVRKWSPFFRTVFFLPVVISMTVIALLFDFIYNPE 129 Query: 132 --VLPQIAEKTAMSFLDQLWIGDPKVSFYSIIIVSLWHGVGYMMIIYLAALQGVPQSLKE 189 +L Q+ + + L + W+GD + S+I VS W VGY+ ++Y+ ++Q +P L E Sbjct: 130 TGLLNQLLQAIGLDQLTRAWLGDDSTAMLSVIFVSQWQSVGYIAMLYIVSIQKIPDELYE 189 Query: 190 AAVIDGASPFQTFRKVTLPMITHAVTICFFLTLNGTFKVFEAVYGLTGGGPGRSTQVITM 249 AA +DGA Q F +T+P + +TL G F VF Y LTGGGPG++++V++ Sbjct: 190 AARLDGAGKIQQFFHITVPQTKEMSFVAVVMTLTGAFTVFNEPYILTGGGPGKASEVLST 249 Query: 250 NIYEEAFSNNFRYGYASAKSVILFVIIL 277 +Y+ AF+ + GYASA + ++ +I L Sbjct: 250 FLYKSAFTKDMM-GYASAIATVVLIITL 276 >PH1215 [G] COG1175 ABC-type sugar transport systems, permease components Length = 292 Score = 155 bits (392), Expect = 7e-38, Method: Compositional matrix adjust. Identities = 95/260 (36%), Positives = 149/260 (57%), Gaps = 5/260 (1%) Query: 12 LLAFVAPAFIIYLLFLLIPTIGGMYYSFTDWNGLNPNYSFTGLGNFIEALREDPDFVNSL 71 ++ F+ PA I+ +F+ + +Y SFTDW GL P+Y F GL + + L DP F SL Sbjct: 10 IILFLIPALILIGIFVYFAVVWNIYISFTDWRGLIPSYHFVGLAQY-KQLIHDPIFWTSL 68 Query: 72 LFTLKYVLFMIVLQNVFALVLAVFIETKTRTKGFFRTIFFMPNMISTIIGAFMWTFVF-- 129 L +L + + L LA+ ++ K R + FRTI+ +P +S ++ A +W +++ Sbjct: 69 RNNLLLILLFVPGSLLLGLFLAILLDMKVRFESGFRTIYVLPFALSFVVTATLWAWMYDP 128 Query: 130 -SQVLPQIAEKTAMSFLDQLWIGDPKVSFYSIIIVSLWHGVGYMMIIYLAALQGVPQSLK 188 S VL + +K + FL WI DPK++ Y III +W GY MIIYLA ++ +P Sbjct: 129 SSGVLNVLFDKLGLDFLKSGWITDPKIAMYCIIIALIWQFSGYTMIIYLAGIRSIPIEQY 188 Query: 189 EAAVIDGASPFQTFRKVTLPMITHAVTICFFLTLNGTFKVFEAVYGLTGGGPGRSTQVIT 248 E A+IDGAS +Q +R + +P +T F + + + K F+ ++ LT GGPG ST ++ Sbjct: 189 EGALIDGASTWQLYRYIVIPQLTKPTLSAFVVLMVFSLKAFDFIWVLTRGGPGTSTFILA 248 Query: 249 MNIYEEAFSN-NFRYGYASA 267 + +Y+E F+ NF YG A A Sbjct: 249 IEMYKETFAKTNFAYGAAIA 268 >YPO1722 [G] COG1175 ABC-type sugar transport systems, permease components Length = 296 Score = 148 bits (373), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 95/271 (35%), Positives = 155/271 (57%), Gaps = 4/271 (1%) Query: 7 KNVLSLLAFVAPAFIIYLLFLLIPTIGGMYYSFTDWNGLNPNYSFTGLGNFIEALREDPD 66 +N + LA+++P I ++F P + + SFT+++ ++P + G+ N+ L ED Sbjct: 3 ENRMLGLAYISPYIIGLIIFTAFPFVSSFFLSFTEYDLMSPP-VYNGIENYRYMLTEDDL 61 Query: 67 FVNSLLFTLKYVLFMIVLQNVFALVLAVFIETKTRTKGFFRTIFFMPNMI-STIIGAFMW 125 F S+ T YV I L+ FAL +A + K R GFFRT F++P+++ S++ A +W Sbjct: 62 FWKSMGVTFAYVFLTIPLKLAFALGIAFVLNFKLRGIGFFRTAFYIPSILGSSVAIAVLW 121 Query: 126 TFVFSQVLPQIAEKTAMSFLDQLWIGDPKVSFYSIIIVSLWHGVGYMMIIYLAALQGVPQ 185 +F+ + F W+G+P ++ S+ ++ +W G M+I+LAALQ VPQ Sbjct: 122 RALFAIDGLLNSFIGVFGFEAINWLGEPSLALTSVTLLRVWQ-FGSAMVIFLAALQNVPQ 180 Query: 186 SLKEAAVIDGASPFQTFRKVTLPMITHAVTICFFLTLNGTFKVFEAVYGLTGGGPGRSTQ 245 S EAA+IDGAS +Q F KVT+P+IT + F + F+ F A Y +TGGGP T Sbjct: 181 SQYEAAMIDGASKWQMFMKVTVPLITPVIFFNFIMQTTQAFQEFTAPYVITGGGPTHYTY 240 Query: 246 VITMNIYEEAFSNNFRYGYASAKSVILFVII 276 + ++ IY+ AF F GY +A + +LF+++ Sbjct: 241 LFSLYIYDTAF-KYFDMGYGAALAWVLFLVV 270 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.330 0.144 0.433 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,869,628 Number of extensions: 797918 Number of successful extensions: 2954 Number of sequences better than 1.0e-05: 184 Number of HSP's gapped: 2669 Number of HSP's successfully gapped: 186 Length of query: 296 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 190 Effective length of database: 26,153,027 Effective search space: 4969075130 Effective search space used: 4969075130 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 115 (48.9 bits)