BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0412.0004.p (282 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PH0820 [V] COG1131 ABC-type multidrug transport system, ATPase c... 182 4e-46 Rv2688c [V] COG1131 ABC-type multidrug transport system, ATPase ... 177 2e-44 MA1596 [V] COG1131 ABC-type multidrug transport system, ATPase c... 174 2e-43 MT2762 [V] COG1131 ABC-type multidrug transport system, ATPase c... 171 1e-42 BS_yfiL [V] COG1131 ABC-type multidrug transport system, ATPase ... 169 4e-42 >PH0820 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 312 Score = 182 bits (463), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 108/307 (35%), Positives = 169/307 (55%), Gaps = 37/307 (12%) Query: 4 AIEAVGMRKAFGDKTVIKNLSFKVKQGEIFGFLGPSGSGKTTTIKMLTGELAPTDGNLEV 63 IEA+ + K +G+ ++ ++F V +GE+FGFLGP+G+GKTTTI+MLTG L PT G + V Sbjct: 7 VIEAINLTKYYGNFPAVRGVTFSVNEGEVFGFLGPNGAGKTTTIRMLTGVLKPTSGQVRV 66 Query: 64 LGM----EPAKFGTADFKSEIGILSDNSSLYERLTVYDNLKLFGKLYGAPLNRIEV---- 115 LG EP K + +GI+ + ++ Y LT NLKL G+LYG P IE Sbjct: 67 LGYDMTREPEKLKA---RQRMGIVPEMANPYIDLTAMQNLKLMGELYGLPKWEIEKRSIE 123 Query: 116 ILREVQLQDELAKTVSKLSRGMKQRVLLAKALIHKPKLLFLDEPTSALDPGNMAHIHRGL 175 +L+ +L ++ V S+GM+QR++LA A+I P+L FLDEPTS LD + I + Sbjct: 124 LLKLFELYEKRNIKVRGFSKGMRQRLVLAMAMIADPELYFLDEPTSGLDVISARIIKDII 183 Query: 176 KKLNEAGTTIFLTTHNMEEATALCDRVAFLHHGELQDLDNPAALRYKYSTH--------- 226 + +AG TIFLTTHNM +A LC+R+ + G+L +D P L+ H Sbjct: 184 RDEKKAGKTIFLTTHNMNDANELCERIGIIRKGKLVAIDTPEGLKRLTKEHLSVEVSLKP 243 Query: 227 ----------AFHIETFAGQRLVIENEPEKADQVSDLIARG-----QVKAMHTDNPTLGQ 271 A +E + + N+P+K V +++ ++ ++ T +P+L Sbjct: 244 MMFDPSVLSSALRVERLGDKVKIYTNDPDKT--VKEIVEYAKERKLKIVSIRTLSPSLED 301 Query: 272 IFLKVTG 278 +F+++ G Sbjct: 302 VFVEIIG 308 >Rv2688c [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 301 Score = 177 bits (448), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 103/268 (38%), Positives = 150/268 (55%), Gaps = 8/268 (2%) Query: 20 IKNLSFKVKQGEIFGFLGPSGSGKTTTIKMLTGELAPTDGNLEVLGMEPAKFGTADFKSE 79 ++ + F V +GEIFG LGPSG+GK+TT K+L G L G V EPA++G D+ Sbjct: 35 VRGMEFTVGRGEIFGLLGPSGAGKSTTQKLLIGLLRDHGGQATVWDKEPAEWG-PDYYER 93 Query: 80 IGILSDNSSLYERLTVYDNLKLFGKLYGAPLNRIEVILREVQLQDELAKTVSKLSRGMKQ 139 IG+ + + Y++LT Y+NL+ F LY +L V L D+ V K S+GM+ Sbjct: 94 IGVSFELPNHYQKLTGYENLRFFASLYAGATADPMQLLAAVGLADDAHTLVGKYSKGMQM 153 Query: 140 RVLLAKALIHKPKLLFLDEPTSALDPGNMAHIHRGLKKLNEAGTTIFLTTHNMEEATALC 199 R+ A++LI+ P+LLFLDEPTS LDP N I + L G TIFLTTH+M A LC Sbjct: 154 RLPFARSLINDPELLFLDEPTSGLDPVNARKIKDIIVDLKARGRTIFLTTHDMATADELC 213 Query: 200 DRVAFLHHGELQDLDNPAALRYKYSTHAFHIETFAGQRLVIENEPEKADQVSD------L 253 DRVAF+ G + LD+P L+ S +E + G +E D ++D + Sbjct: 214 DRVAFVVDGRIVALDSPTELKIARSRRRVRVE-YRGDGGGLETAEFGMDGLADDPAFHSV 272 Query: 254 IARGQVKAMHTDNPTLGQIFLKVTGNEL 281 + V+ +H+ +L +F++VTG +L Sbjct: 273 LRNHHVETIHSREASLDDVFVEVTGRQL 300 >MA1596 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 318 Score = 174 bits (440), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 108/307 (35%), Positives = 167/307 (54%), Gaps = 35/307 (11%) Query: 4 AIEAVGMRKAFGDKTVIKNLSFKVKQGEIFGFLGPSGSGKTTTIKMLTGELAPTDGNLEV 63 AIE + K FG T + ++SF V +GE FGFLGP+G+GKTTTI++LTG PT G + Sbjct: 3 AIEVSHLIKKFGSLTALNDISFSVGEGETFGFLGPNGAGKTTTIRILTGVSRPTSGTATI 62 Query: 64 LGMEPAKFGTADFKSEIGILSDNSSLYERLTVYDNLKLFGKLYGAPLNRIEV----ILRE 119 G + K T + +GI+S+NS++Y+ LT + NL +LY E +LR+ Sbjct: 63 FGHD-VKHDTIAARQSVGIVSENSNVYDDLTAWQNLMFSAELYHVGRKEREKKANELLRK 121 Query: 120 VQLQDELAKTVSKLSRGMKQRVLLAKALIHKPKLLFLDEPTSALDPGNMAHIHRGLKKLN 179 +L + V S+GMK+R+ LA +++ P+L+FLDEPTS LD + I + L Sbjct: 122 FELYERRNDKVQGFSKGMKRRLTLAMGMVNSPRLIFLDEPTSGLDVQSNLIIREVISGLV 181 Query: 180 EAGTTIFLTTHNMEEATALCDRVAFLHHGELQDLDNPAALRYKYST-----HAF------ 228 G T+FLTTHN+EEA +CDRVA ++ G + +D P +L+ + AF Sbjct: 182 REGVTVFLTTHNIEEANVMCDRVAIINKGNIVAVDTPESLKRTIRSVQSIEVAFDNNSID 241 Query: 229 HIETFAGQRLVIENEPEKA------------DQVSDLIARG-----QVKAMHTDNPTLGQ 271 H+E + RL + NE KA + + L+ ++ ++ T P+L Sbjct: 242 HLEELS--RLPMINEVHKAGDKFKIFTELPSEVIDALVVYAHSYNLKILSIATPGPSLED 299 Query: 272 IFLKVTG 278 +F+++TG Sbjct: 300 VFVRLTG 306 >MT2762 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 317 Score = 171 bits (433), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 102/261 (39%), Positives = 146/261 (55%), Gaps = 8/261 (3%) Query: 27 VKQGEIFGFLGPSGSGKTTTIKMLTGELAPTDGNLEVLGMEPAKFGTADFKSEIGILSDN 86 V +GEIFG LGPSG+GK+TT K+L G L G V EPA++G D+ IG+ + Sbjct: 58 VGRGEIFGLLGPSGAGKSTTQKLLIGLLRDHGGQATVWDKEPAEWG-PDYYERIGVSFEL 116 Query: 87 SSLYERLTVYDNLKLFGKLYGAPLNRIEVILREVQLQDELAKTVSKLSRGMKQRVLLAKA 146 + Y++LT Y+NL+ F LY +L V L D+ V K S+GM+ R+ A++ Sbjct: 117 PNHYQKLTGYENLRFFASLYAGATADPMQLLAAVGLADDAHTLVGKYSKGMQMRLTFARS 176 Query: 147 LIHKPKLLFLDEPTSALDPGNMAHIHRGLKKLNEAGTTIFLTTHNMEEATALCDRVAFLH 206 LI+ P+LLFLDEPTS LDP N I + L G TIFLTTH+M A LCDRVAF+ Sbjct: 177 LINDPELLFLDEPTSGLDPVNARKIKDIIVDLKARGRTIFLTTHDMATADELCDRVAFVV 236 Query: 207 HGELQDLDNPAALRYKYSTHAFHIETFAGQRLVIENEPEKADQVSD------LIARGQVK 260 G + LD+P L+ S +E + G +E D ++D ++ V+ Sbjct: 237 DGRIVALDSPTELKIARSRRRVRVE-YRGDGGGLETAEFGMDGLADDPAFHSVLRNHHVE 295 Query: 261 AMHTDNPTLGQIFLKVTGNEL 281 +H+ +L +F++VTG +L Sbjct: 296 TIHSREASLDDVFVEVTGRQL 316 >BS_yfiL [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 134 Score = 169 bits (428), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 0/210 (0%), Positives = 0/210 (0%), Gaps = 5/210 (2%) Query: 5 IEAVGMRKAFGDKTVIKNLSFKVKQGEIFGFLGPSGSGKTTTIKMLTGELAPTDGNLEVL 64 K + + + T+ + + Sbjct: 2 KTIRYALKKEKEMMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISGSAIK 61 Query: 65 GMEPAKFGTADFKSEIGILSDNSSLYERLTVYDNLKLFGKLYGAPLN----RIEVILREV 120 + + + T + + + Sbjct: 62 DDDAFS-DSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQLHEVW 120 Query: 121 QLQDELAKTVSKLSRGMKQRVLLAKALIHKPKLLFLDEPTSALDPGNMAHIHRGLKKLNE 180 Sbjct: 121 180 Query: 181 AGTTIFLTTHNMEEATALCDRVAFLHHGEL 210 Sbjct: 181 210 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.317 0.136 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,554,676 Number of extensions: 830004 Number of successful extensions: 10081 Number of sequences better than 1.0e-05: 2182 Number of HSP's gapped: 6860 Number of HSP's successfully gapped: 2452 Length of query: 282 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 177 Effective length of database: 26,268,361 Effective search space: 4649499897 Effective search space used: 4649499897 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 115 (48.9 bits)