BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0407.0021.p (112 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DR0033 [J] COG0251 Putative translation initiation inhibitor, yj... 132 1e-31 Rv2704 [J] COG0251 Putative translation initiation inhibitor, yj... 108 3e-24 MT2777.1 [J] COG0251 Putative translation initiation inhibitor, ... 108 3e-24 mll4450 [J] COG0251 Putative translation initiation inhibitor, y... 59 1e-09 mll4402 [J] COG0251 Putative translation initiation inhibitor, y... 59 2e-09 >DR0033 [J] COG0251 Putative translation initiation inhibitor, yjgF family Length = 141 Score = 132 bits (332), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 0/109 (0%), Positives = 0/109 (0%) Query: 1 VGYCRAIRIGDRIEVAGTTAIKDGKVFGKGSPYEQTRFILQTIEKALGELGAGLDNVVRT 60 G + + K + ++ + V Sbjct: 43 SGQIPVNPKTGEVPADVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNA 102 Query: 61 RMFVTDISKWEEFGKAHGEFFKEIQPVATMVEVKALIDPDLLIEIEVEA 109 Sbjct: 103 151 >Rv2704 [J] COG0251 Putative translation initiation inhibitor, yjgF family Length = 141 Score = 108 bits (269), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 9/109 (8%), Positives = 19/109 (17%), Gaps = 7/109 (6%) Query: 1 VGYCRAIRIGDRIEVAGTTAIKDGKVFGKGSPYEQTRFILQTIEKALGELGAGLDNVVRT 60 + A Q +T E ++ Sbjct: 18 IATENAPAAIGPYVQGVDLGNMIITS-------GQIPVNPKTGEVPADVAAQARQSLDNV 70 Query: 61 RMFVTDISKWEEFGKAHGEFFKEIQPVATMVEVKALIDPDLLIEIEVEA 109 + V F K++ AT+ + + Sbjct: 71 KAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATFPARS 119 >MT2777.1 [J] COG0251 Putative translation initiation inhibitor, yjgF family Length = 141 Score = 108 bits (269), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 9/109 (8%), Positives = 19/109 (17%), Gaps = 7/109 (6%) Query: 1 VGYCRAIRIGDRIEVAGTTAIKDGKVFGKGSPYEQTRFILQTIEKALGELGAGLDNVVRT 60 + A Q +T E ++ Sbjct: 18 IATENAPAAIGPYVQGVDLGNMIITS-------GQIPVNPKTGEVPADVAAQARQSLDNV 70 Query: 61 RMFVTDISKWEEFGKAHGEFFKEIQPVATMVEVKALIDPDLLIEIEVEA 109 + V F K++ AT+ + + Sbjct: 71 KAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATFPARS 119 >mll4450 [J] COG0251 Putative translation initiation inhibitor, yjgF family Length = 141 Score = 59.3 bits (142), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 2/106 (1%), Positives = 11/106 (10%), Gaps = 4/106 (3%) Query: 6 AIRIGDRIEVAGTTAIKDGKVFGKGSPYEQTRFILQTIEKALGELGAGLDNVVRTRMFVT 65 +++ + Q + ++ V Sbjct: 27 IGPYVQGVDLGNMIITSGQIPVNPKTGEVPADVAAQARQSLDNVKAIVEAAGLKVGDIVK 86 Query: 66 DISKWEEFGKAHGEFFKEIQPVATMVEVKALIDPDLLIEIEVEAAA 111 ++ Sbjct: 87 TTVFVKDLNDFATVNATYEAFFTEHNATFPARS----CVEVARLPK 128 >mll4402 [J] COG0251 Putative translation initiation inhibitor, yjgF family Length = 141 Score = 58.9 bits (141), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 5/108 (4%), Positives = 13/108 (12%), Gaps = 1/108 (0%) Query: 2 GYCRAIRIGDRIEVAGTTAIKDGKVFGKGSPYEQTRFILQTIEKALGELGAGLDNVVRTR 61 I + G + + A + + Sbjct: 16 KTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPADVAAQARQSLDNVKAIVE 75 Query: 62 MFVTDISKWEEFGKAHGEFFKEIQPVATMVEVKALIDPDLLIEIEVEA 109 + + + AT Sbjct: 76 AAGLKVGDIVKTTVFVKDLNDFATVNATYE-AFFTEHNATFPARSCVE 122 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.320 0.140 0.400 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 8,309,679 Number of extensions: 339033 Number of successful extensions: 792 Number of sequences better than 1.0e-05: 25 Number of HSP's gapped: 796 Number of HSP's successfully gapped: 25 Length of query: 112 Length of database: 38,378,431 Length adjustment: 79 Effective length of query: 33 Effective length of database: 29,267,045 Effective search space: 965812485 Effective search space used: 965812485 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 109 (46.6 bits)