BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0407.0007.p (358 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0797 [E] COG3842 ABC-type spermidine/putrescine transport sys... 533 e-151 SP1389 [E] COG3842 ABC-type spermidine/putrescine transport syst... 474 e-133 potA [E] COG3842 ABC-type spermidine/putrescine transport system... 265 8e-71 ZpotA [E] COG3842 ABC-type spermidine/putrescine transport syste... 265 8e-71 >lin0797 [E] COG3842 ABC-type spermidine/putrescine transport systems, ATPase components Length = 182 Score = 533 bits (1372), Expect = e-151, Method: Compositional matrix adjust. Identities = 0/354 (0%), Positives = 0/354 (0%) Query: 1 VIKQYDDEEPVLKGVSFEIERGKFYTLLGPSGCGKTTILRLIAGFAEPTKGSIYLNGQII 60 + + ++ + G+ + R Sbjct: 11 AQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKGQLLVTRRALSKKAWPGVWTNSVCGHP 70 Query: 61 NHIPANERQVNTVFQDYALFPHLNVFENVAFGLRIKKLKKDVITHKVQEALRFVNLVGYE 120 +NE V + ++ ++V + Sbjct: 71 QLGESNEDAVIRRCRYELGVEITPPESIYPDFRYRATDPSGIVENEVCPVFAARTTSALQ 130 Query: 121 QRAINEMSGGQRQRVAIARAIVNEPQVXXXXXXXXXXXXXXRTEMQYILREMQQRLGITF 180 Sbjct: 131 190 Query: 181 IFVTHDQEEALAMSDWIFVMNGGRIEQSGTPNDIYDEPINRFVADFIGESNIVPGVMIED 240 Sbjct: 191 250 Query: 241 YLVEFNGHRFECVDAGLRRNEAVEIVIRPEDLEIAALEQGKLRVRVDSQLFRGVHYEISC 300 Sbjct: 251 310 Query: 301 YDESGHEWLVHSTRKAEVGSEIGLYFDPEAIHVMRFGETEEEFDKRLEAYGEVE 354 Sbjct: 311 364 >SP1389 [E] COG3842 ABC-type spermidine/putrescine transport systems, ATPase components Length = 385 Score = 474 bits (1220), Expect = e-133, Method: Compositional matrix adjust. Identities = 229/342 (66%), Positives = 268/342 (78%) Query: 6 DDEEPVLKGVSFEIERGKFYTLLGPSGCGKTTILRLIAGFAEPTKGSIYLNGQIINHIPA 65 D VLK ++FE+E GKFYTLLG SG GK+TIL +IAG + T G I L+G IN IP Sbjct: 17 DSNTKVLKDINFELEEGKFYTLLGASGSGKSTILNIIAGLLDATTGDIMLDGVRINDIPT 76 Query: 66 NERQVNTVFQDYALFPHLNVFENVAFGLRIKKLKKDVITHKVQEALRFVNLVGYEQRAIN 125 N+R V+TVFQ YALFPH+NVFENVAF LR++K+ K I +V E L+ V L GYE+R+I Sbjct: 77 NKRDVHTVFQSYALFPHMNVFENVAFPLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIR 136 Query: 126 EMSGGQRQRVAIARAIVNEPQVXXXXXXXXXXXXXXRTEMQYILREMQQRLGITFIFVTH 185 ++SGGQRQRVAIARAI+N+P+V RT+MQY LRE+QQRLGITF+FVTH Sbjct: 137 KLSGGQRQRVAIARAIINQPRVVLLDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTH 196 Query: 186 DQEEALAMSDWIFVMNGGRIEQSGTPNDIYDEPINRFVADFIGESNIVPGVMIEDYLVEF 245 DQEEALAMSDWIFVMN G I QSGTP DIYDEPIN FVA FIGESNI+PG MIEDYLVEF Sbjct: 197 DQEEALAMSDWIFVMNDGEIVQSGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEF 256 Query: 246 NGHRFECVDAGLRRNEAVEIVIRPEDLEIAALEQGKLRVRVDSQLFRGVHYEISCYDESG 305 NG RFE VD G++ NE VE+VIRPEDL I E+GKL+V+VD+QLFRGVHYEI YDE G Sbjct: 257 NGKRFEAVDGGMKPNEPVEVVIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELG 316 Query: 306 HEWLVHSTRKAEVGSEIGLYFDPEAIHVMRFGETEEEFDKRL 347 +EW++HSTRKA VG EIGL F+PE IH+MR ETEEEFD R+ Sbjct: 317 NEWMIHSTRKAIVGEEIGLDFEPEDIHIMRLNETEEEFDARI 358 >potA [E] COG3842 ABC-type spermidine/putrescine transport systems, ATPase components Length = 378 Score = 265 bits (677), Expect = 8e-71, Method: Compositional matrix adjust. Identities = 140/279 (50%), Positives = 182/279 (65%), Gaps = 6/279 (2%) Query: 2 IKQYDDEEPVLKGVSFEIERGKFYTLLGPSGCGKTTILRLIAGFAEPTKGSIYLNGQIIN 61 I++ D + V+ + I G+F TLLGPSGCGKTT+LRLIAG G I L+ + I Sbjct: 23 IRKCFDGKEVIPQLDLTINNGEFLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDIT 82 Query: 62 HIPANERQVNTVFQDYALFPHLNVFENVAFGLRIKKLKKDVITHKVQEALRFVNLVGYEQ 121 H+PA R VNTVFQ YALFPH+ VFENVAFGLR++K IT +V EALR V L + Q Sbjct: 83 HVPAENRYVNTVFQSYALFPHMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQ 142 Query: 122 RAINEMSGGQRQRVAIARAIVNEPQVXXXXXXXXXXXXXXRTEMQYILREMQQRLGITFI 181 R +++SGGQ+QRVAIARA+VN+P++ R +MQ L+ +Q++LGITF+ Sbjct: 143 RKPHQLSGGQQQRVAIARAVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFV 202 Query: 182 FVTHDQEEALAMSDWIFVMNGGRIEQSGTPNDIYDEPINRFVADFIGESNIVPGVMIE-- 239 FVTHDQEEAL MSD I VM GRIEQ GTP +IY+EP N FVA FIGE N+ +IE Sbjct: 203 FVTHDQEEALTMSDRIVVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERL 262 Query: 240 -DYLVEFNGHRFEC---VDAGLRRNEAVEIVIRPEDLEI 274 + V N EC V+ + + + +++RPEDL + Sbjct: 263 DEQRVRANVEGRECNIYVNFAVEPGQKLHVLLRPEDLRV 301 >ZpotA [E] COG3842 ABC-type spermidine/putrescine transport systems, ATPase components Length = 378 Score = 265 bits (677), Expect = 8e-71, Method: Compositional matrix adjust. Identities = 140/279 (50%), Positives = 182/279 (65%), Gaps = 6/279 (2%) Query: 2 IKQYDDEEPVLKGVSFEIERGKFYTLLGPSGCGKTTILRLIAGFAEPTKGSIYLNGQIIN 61 I++ D + V+ + I G+F TLLGPSGCGKTT+LRLIAG G I L+ + I Sbjct: 23 IRKCFDGKEVIPQLDLTINNGEFLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDIT 82 Query: 62 HIPANERQVNTVFQDYALFPHLNVFENVAFGLRIKKLKKDVITHKVQEALRFVNLVGYEQ 121 H+PA R VNTVFQ YALFPH+ VFENVAFGLR++K IT +V EALR V L + Q Sbjct: 83 HVPAENRYVNTVFQSYALFPHMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQ 142 Query: 122 RAINEMSGGQRQRVAIARAIVNEPQVXXXXXXXXXXXXXXRTEMQYILREMQQRLGITFI 181 R +++SGGQ+QRVAIARA+VN+P++ R +MQ L+ +Q++LGITF+ Sbjct: 143 RKPHQLSGGQQQRVAIARAVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFV 202 Query: 182 FVTHDQEEALAMSDWIFVMNGGRIEQSGTPNDIYDEPINRFVADFIGESNIVPGVMIE-- 239 FVTHDQEEAL MSD I VM GRIEQ GTP +IY+EP N FVA FIGE N+ +IE Sbjct: 203 FVTHDQEEALTMSDRIVVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERL 262 Query: 240 -DYLVEFNGHRFEC---VDAGLRRNEAVEIVIRPEDLEI 274 + V N EC V+ + + + +++RPEDL + Sbjct: 263 DEQRVRANVEGRECNIYVNFAVEPGQKLHVLLRPEDLRV 301 Score = 265 bits (677), Expect = 8e-71, Method: Compositional matrix adjust. Identities = 140/279 (50%), Positives = 182/279 (65%), Gaps = 6/279 (2%) Query: 2 IKQYDDEEPVLKGVSFEIERGKFYTLLGPSGCGKTTILRLIAGFAEPTKGSIYLNGQIIN 61 I++ D + V+ + I G+F TLLGPSGCGKTT+LRLIAG G I L+ + I Sbjct: 23 IRKCFDGKEVIPQLDLTINNGEFLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDIT 82 Query: 62 HIPANERQVNTVFQDYALFPHLNVFENVAFGLRIKKLKKDVITHKVQEALRFVNLVGYEQ 121 H+PA R VNTVFQ YALFPH+ VFENVAFGLR++K IT +V EALR V L + Q Sbjct: 83 HVPAENRYVNTVFQSYALFPHMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQ 142 Query: 122 RAINEMSGGQRQRVAIARAIVNEPQVXXXXXXXXXXXXXXRTEMQYILREMQQRLGITFI 181 R +++SGGQ+QRVAIARA+VN+P++ R +MQ L+ +Q++LGITF+ Sbjct: 143 RKPHQLSGGQQQRVAIARAVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFV 202 Query: 182 FVTHDQEEALAMSDWIFVMNGGRIEQSGTPNDIYDEPINRFVADFIGESNIVPGVMIE-- 239 FVTHDQEEAL MSD I VM GRIEQ GTP +IY+EP N FVA FIGE N+ +IE Sbjct: 203 FVTHDQEEALTMSDRIVVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERL 262 Query: 240 -DYLVEFNGHRFEC---VDAGLRRNEAVEIVIRPEDLEI 274 + V N EC V+ + + + +++RPEDL + Sbjct: 263 DEQRVRANVEGRECNIYVNFAVEPGQKLHVLLRPEDLRV 301 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.139 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 26,588,760 Number of extensions: 1139898 Number of successful extensions: 9032 Number of sequences better than 1.0e-05: 2045 Number of HSP's gapped: 6230 Number of HSP's successfully gapped: 2203 Length of query: 358 Length of database: 38,378,431 Length adjustment: 108 Effective length of query: 250 Effective length of database: 25,922,359 Effective search space: 6480589750 Effective search space used: 6480589750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)