BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0407.0006.p (268 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value potB [E] COG1176 ABC-type spermidine/putrescine transport system... 145 9e-35 ZpotB [E] COG1176 ABC-type spermidine/putrescine transport syste... 145 9e-35 ECs1570 [E] COG1176 ABC-type spermidine/putrescine transport sys... 144 1e-34 STM1225 [E] COG1176 ABC-type spermidine/putrescine transport sys... 143 3e-34 >potB [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 275 Score = 145 bits (365), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 84/259 (32%), Positives = 138/259 (53%), Gaps = 10/259 (3%) Query: 16 WIALFVIAPVLLVVYYSLFDLDGH------LTLDNYVNFFTPVYMKMMLNSFWYAFLITL 69 W+ LFV P L+++ S D TLDNY P+Y +++L+S A + TL Sbjct: 8 WLVLFVFLPNLMIIGTSFLTRDDASFVKMVFTLDNYTRLLDPLYFEVLLHSLNMALIATL 67 Query: 70 FSLLVAYPAAYLLTRTKHK--QXXXXXXXXXXXXXXXXKTYAFIGIFGTFGPVNHFFGLL 127 L++ YP A+ L + HK + Y T G +N F L Sbjct: 68 ACLVLGYPFAWFLAKLPHKVRPLLLFLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWL 127 Query: 128 GLGEQ--QILFTGXXXXXXXXYIFIPFMILPIFSALEELNLSLVDAARDLGASGWTTFRR 185 G+ + +I+FT YI +PFM++P++S++E+L+ L++AARDLGAS TF R Sbjct: 128 GVIDTPIRIMFTPSAVIIGLVYILLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIR 187 Query: 186 VIFPLTISGVRSGCMAVFIPALSLFMITRLIAGNRVITLGTAIEQHFLVTQDWGMGSTVA 245 +I PLT+ G+ +GC+ V +PA+ LF ++ L+ G + + +G I+ FL +DW G+ + Sbjct: 188 IIIPLTMPGIIAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATS 247 Query: 246 VFLIVIMALFMLMTGSSRR 264 + L ++M L +L+ + R Sbjct: 248 ITLTIVMGLMLLVYWRASR 266 >ZpotB [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 275 Score = 145 bits (365), Expect = 9e-35, Method: Compositional matrix adjust. Identities = 84/259 (32%), Positives = 138/259 (53%), Gaps = 10/259 (3%) Query: 16 WIALFVIAPVLLVVYYSLFDLDGH------LTLDNYVNFFTPVYMKMMLNSFWYAFLITL 69 W+ LFV P L+++ S D TLDNY P+Y +++L+S A + TL Sbjct: 8 WLVLFVFLPNLMIIGTSFLTRDDASFVKMVFTLDNYTRLLDPLYFEVLLHSLNMALIATL 67 Query: 70 FSLLVAYPAAYLLTRTKHK--QXXXXXXXXXXXXXXXXKTYAFIGIFGTFGPVNHFFGLL 127 L++ YP A+ L + HK + Y T G +N F L Sbjct: 68 ACLVLGYPFAWFLAKLPHKVRPLLLFLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWL 127 Query: 128 GLGEQ--QILFTGXXXXXXXXYIFIPFMILPIFSALEELNLSLVDAARDLGASGWTTFRR 185 G+ + +I+FT YI +PFM++P++S++E+L+ L++AARDLGAS TF R Sbjct: 128 GVIDTPIRIMFTPSAVIIGLVYILLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIR 187 Query: 186 VIFPLTISGVRSGCMAVFIPALSLFMITRLIAGNRVITLGTAIEQHFLVTQDWGMGSTVA 245 +I PLT+ G+ +GC+ V +PA+ LF ++ L+ G + + +G I+ FL +DW G+ + Sbjct: 188 IIIPLTMPGIIAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATS 247 Query: 246 VFLIVIMALFMLMTGSSRR 264 + L ++M L +L+ + R Sbjct: 248 ITLTIVMGLMLLVYWRASR 266 Score = 144 bits (364), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 0/276 (0%), Positives = 0/276 (0%), Gaps = 12/276 (4%) Query: 1 MKNKGK--AYFLIPYYLWIALFVIAPVLLVVYYSLFDLDGH------LTLDNYVNFFTPV 52 M + + + + L V + Sbjct: 1 MIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFVKMVFTLDNYTRLLDPLYFEVLLHSLN 60 Query: 53 YMKMMLNSFWYAFLITLFSLLVAYPAAYLLTRTKHK--QXXXXXXXXXXXXXXXXKTYAF 110 + + + L L Sbjct: 61 MALIATLACLVLGYPFAWFLAKLPHKVRPLLLFLLIVPFWTNSLIRIYGLKIFLSTKGYL 120 Query: 111 IGIFGTFGPVNHFFGLLGLGEQ--QILFTGXXXXXXXXYIFIPFMILPIFSALEELNLSL 168 G ++ ++ L + + Sbjct: 121 NEFLLWLGVIDTPIRIMFTPSAVIIGLVYILLPFMVMPLYSSIEKLDKPLLEAARDLGAS 180 Query: 169 VDAARDLGASGWTTFRRVIFPLTISGVRSGCMAVFIPALSLFMITRLIAGNRVITLGTAI 228 T + L + G V + Sbjct: 181 KLQTFIRIIIPLTMPGIIAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDW 240 Query: 229 EQHFLVTQDWGMGSTVAVFLIVIMALFMLMTGSSRR 264 Sbjct: 241 276 >ECs1570 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 287 Score = 144 bits (364), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 88/276 (31%), Positives = 144/276 (52%), Gaps = 12/276 (4%) Query: 1 MKNKGK--AYFLIPYYLWIALFVIAPVLLVVYYSLFDLDGH------LTLDNYVNFFTPV 52 MKN K ++ W+ LFV P L+++ S D TLDNY P+ Sbjct: 1 MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLARDDASFVKMVFTLDNYTRLLDPL 60 Query: 53 YMKMMLNSFWYAFLITLFSLLVAYPAAYLLTRTKHK--QXXXXXXXXXXXXXXXXKTYAF 110 Y +++L+S A + TL L++ YP A+ L + HK + Y Sbjct: 61 YFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLLFLLIVPFWTNSLIRIYGL 120 Query: 111 IGIFGTFGPVNHFFGLLGLGEQ--QILFTGXXXXXXXXYIFIPFMILPIFSALEELNLSL 168 T G +N F LG+ + +I+FT YI +PFM++P++S++E+L+ L Sbjct: 121 KIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYILLPFMVMPLYSSIEKLDKPL 180 Query: 169 VDAARDLGASGWTTFRRVIFPLTISGVRSGCMAVFIPALSLFMITRLIAGNRVITLGTAI 228 ++AARDLGAS TF R+I PLT+ G+ +GC+ V +PA+ LF ++ L+ G + + +G I Sbjct: 181 LEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVI 240 Query: 229 EQHFLVTQDWGMGSTVAVFLIVIMALFMLMTGSSRR 264 + FL +DW G+ ++ L ++M L +L+ + R Sbjct: 241 KVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR 276 >STM1225 [E] COG1176 ABC-type spermidine/putrescine transport system, permease component I Length = 287 Score = 143 bits (360), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 87/276 (31%), Positives = 144/276 (52%), Gaps = 12/276 (4%) Query: 1 MKNKGK--AYFLIPYYLWIALFVIAPVLLVVYYSLFDLDGH------LTLDNYVNFFTPV 52 MKN K ++ W+ LFV P L+++ S D TLDNY P+ Sbjct: 1 MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFVKMVFTLDNYARLLDPL 60 Query: 53 YMKMMLNSFWYAFLITLFSLLVAYPAAYLLTR--TKHKQXXXXXXXXXXXXXXXXKTYAF 110 Y +++L+S A + TL L++ YP A+ L + K + + Y Sbjct: 61 YFEVLLHSLNMALIATLSCLVLGYPFAWFLAKLPEKIRPLLLFLLIVPFWTNSLIRIYGL 120 Query: 111 IGIFGTFGPVNHFFGLLGLGEQ--QILFTGXXXXXXXXYIFIPFMILPIFSALEELNLSL 168 T G +N F LG+ + +I+FT YI +PFM++P++S++E+L+ L Sbjct: 121 KIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYILLPFMVMPLYSSIEKLDKPL 180 Query: 169 VDAARDLGASGWTTFRRVIFPLTISGVRSGCMAVFIPALSLFMITRLIAGNRVITLGTAI 228 ++AARDLGAS TF R+I PLT+ G+ +GC+ V +PA+ LF ++ L+ G + + +G I Sbjct: 181 LEAARDLGASKMQTFIRIIIPLTMPGIVAGCLLVMLPAMGLFYVSDLMGGAKNLLIGNVI 240 Query: 229 EQHFLVTQDWGMGSTVAVFLIVIMALFMLMTGSSRR 264 + FL +DW G+ ++ L ++M L +L+ + R Sbjct: 241 KVQFLNIRDWPFGAATSITLTIVMGLMLLIYWRASR 276 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.333 0.146 0.454 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 16,742,116 Number of extensions: 572699 Number of successful extensions: 2895 Number of sequences better than 1.0e-05: 86 Number of HSP's gapped: 2843 Number of HSP's successfully gapped: 87 Length of query: 268 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 163 Effective length of database: 26,268,361 Effective search space: 4281742843 Effective search space used: 4281742843 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.5 bits) S2: 115 (48.9 bits)