BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0407.0005.p (275 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PA0324 [E] COG1177 ABC-type spermidine/putrescine transport syst... 129 5e-30 PA3609 [E] COG1177 ABC-type spermidine/putrescine transport syst... 124 2e-28 STM1378 [G] COG0469 Pyruvate kinase 123 3e-28 STM1223 [E] COG1177 ABC-type spermidine/putrescine transport sys... 122 8e-28 potC [E] COG1177 ABC-type spermidine/putrescine transport system... 120 3e-27 >PA0324 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 262 Score = 129 bits (324), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 75/236 (31%), Positives = 121/236 (51%), Gaps = 1/236 (0%) Query: 22 PILYLMYYSFNSGGTMHKFESFTLDYYREVLGDTRLMIIVINXXXXXXXXXXXXXXXXXX 81 PIL L+ SFNSG + +ESF+L +Y V D ++ N Sbjct: 27 PILVLVVLSFNSGQSATLWESFSLKWYAVVANDPEILRAAKNSLLVATLATLASTVLATL 86 Query: 82 XXXXXQRVRSRRAKNTLLSLNNVLIVSPDVIIGASFLILFTIVGIKLGFTSVLLSHVAFS 141 R R+ R + + + + ++ P+++ + L+ F+ +G+KL ++L++H+ F Sbjct: 87 AALG-MRGRAFRGQTLMNGVLGLPLLVPEIVTAVATLMFFSFIGLKLSLFTILIAHIVFC 145 Query: 142 VPIAVLMILPHLQEMSPTLTDAARDLGASRRDVLTKVVLPIIKPGIFSGFFMALTYSLDD 201 +P A L I L+ + P L +AA DL AS K+ LP++ PG+ SG +A S+DD Sbjct: 146 IPFAYLPIRARLEGLDPRLAEAAADLYASPWKAFWKITLPLLMPGVLSGAMLAFIISMDD 205 Query: 202 FAVTFFVTGNGYSTLSVEIYSRARQGVSLSINALSXXXXXXXXXXVIGYYFLNRRK 257 F +T+FV G G +TL V I+S R G+S INA+S V Y+L +R+ Sbjct: 206 FVITYFVAGAGATTLPVYIFSSIRMGISPKINAISSIILMISIAFVALSYYLGQRR 261 >PA3609 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 256 Score = 124 bits (311), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 72/215 (33%), Positives = 113/215 (52%), Gaps = 4/215 (1%) Query: 22 PILYLMYYSFNSGGTMHKFESFTLDYYREVLGDTRLMIIVINXXXXXXXXXXXXXXXXXX 81 P++ ++ YSFN ++ FTLD+YR++ G+ ++ N Sbjct: 21 PLVIVVLYSFNDSRLNAEWVGFTLDWYRKLFGNRDMLQAAANSLVIALVSASVATLLGTL 80 Query: 82 XXXXXQRVRSRRAKNTLLSLNNVLIVSPDVIIGASFLILFTIVGIKLGFTSVLLSHVAFS 141 R + R LL L + I P++++G S +I F + + LG SV L+H+ FS Sbjct: 81 AGVALHRFKLRVLP--LLVLTPIAI--PELLMGVSLVIFFIGLNMSLGMLSVTLAHITFS 136 Query: 142 VPIAVLMILPHLQEMSPTLTDAARDLGASRRDVLTKVVLPIIKPGIFSGFFMALTYSLDD 201 V L++ + M +LT+AARDLGA+ V LP+I PGI +G MA T S+DD Sbjct: 137 VGFVALVVQARMSGMDESLTEAARDLGATPWQSFHLVTLPLIMPGIVAGALMAFTLSIDD 196 Query: 202 FAVTFFVTGNGYSTLSVEIYSRARQGVSLSINALS 236 F +TFF G G++TL +IY+ + V+ +NA+S Sbjct: 197 FVITFFTAGGGFATLPTQIYTMIKIAVTPEVNAVS 231 >STM1378 [G] COG0469 Pyruvate kinase Length = 470 Score = 123 bits (309), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 8/147 (5%), Positives = 24/147 (16%) Query: 90 RSRRAKNTLLSLNNVLIVSPDVIIGASFLILFTIVGIKLGFTSVLLSHVAFSVPIAVLMI 149 T S+ + G + + + + + ++ Sbjct: 88 GQTFTFTTDKSVVGNNEIVAVTYEGFTSDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVL 147 Query: 150 LPHLQEMSPTLTDAARDLGASRRDVLTKVVLPIIKPGIFSGFFMALTYSLDDFAVTFFVT 209 + + + K L + D Sbjct: 148 NNGDLGENKGVNLPGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVVEIREHL 207 Query: 210 GNGYSTLSVEIYSRARQGVSLSINALS 236 I Q + + + Sbjct: 208 KAHGGENIQIISKIENQEGLNNFDEIL 234 >STM1223 [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 182 Score = 122 bits (305), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 0/230 (0%), Positives = 0/230 (0%), Gaps = 1/230 (0%) Query: 30 SFNSGGTMHKFESFTLDYYREVLGDTRLMIIVINXXXXXXXXXXXXXXXXXXXXXXXQRV 89 + + ++ + + Sbjct: 29 TRLHLAFSSWLFNAKGQLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYEL 88 Query: 90 RSRRAKNTLLSLNNVLIVSPDVIIGASFLILFTIVGIKLGFTSVLLSHVAFSVPIAVLMI 149 + + D + + V + + Sbjct: 89 G-VEITPPESIYPDFRYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADV 147 Query: 150 LPHLQEMSPTLTDAARDLGASRRDVLTKVVLPIIKPGIFSGFFMALTYSLDDFAVTFFVT 209 Sbjct: 148 207 Query: 210 GNGYSTLSVEIYSRARQGVSLSINALSXXXXXXXXXXVIGYYFLNRRKNR 259 Sbjct: 208 257 >potC [E] COG1177 ABC-type spermidine/putrescine transport system, permease component II Length = 264 Score = 120 bits (300), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 70/230 (30%), Positives = 117/230 (50%), Gaps = 1/230 (0%) Query: 30 SFNSGGTMHKFESFTLDYYREVLGDTRLMIIVINXXXXXXXXXXXXXXXXXXXXXXXQRV 89 SFNS ++ FT +Y ++ + L+ + R Sbjct: 29 SFNSSRFGINWQGFTTKWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRY 88 Query: 90 RSRRAKNTLLSLNNVLIVSPDVIIGASFLILFTIVGIKLGFTSVLLSHVAFSVPIAVLMI 149 R R K + + V+++SPD+++ S L+LF ++GI+LGF S+L SH+ F +P V+ + Sbjct: 89 R-FRGKPFVSGMLFVVMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTV 147 Query: 150 LPHLQEMSPTLTDAARDLGASRRDVLTKVVLPIIKPGIFSGFFMALTYSLDDFAVTFFVT 209 L+ + +AA+DLGAS +L K++LP+ P + +G+ ++ T S+DD V+ FVT Sbjct: 148 YSRLKGFDVRMLEAAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVT 207 Query: 210 GNGYSTLSVEIYSRARQGVSLSINALSXXXXXXXXXXVIGYYFLNRRKNR 259 G Y L ++IYS + GVS +NAL+ VI + R K + Sbjct: 208 GPSYEILPLKIYSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTK 257 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.325 0.141 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,531,587 Number of extensions: 487138 Number of successful extensions: 2039 Number of sequences better than 1.0e-05: 81 Number of HSP's gapped: 2039 Number of HSP's successfully gapped: 82 Length of query: 275 Length of database: 38,378,431 Length adjustment: 105 Effective length of query: 170 Effective length of database: 26,268,361 Effective search space: 4465621370 Effective search space used: 4465621370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 115 (48.9 bits)