BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0405.0010.p (543 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value DR0258 [GEPR] COG0477 Permeases of the major facilitator superfa... 285 9e-77 lin0980 [GEPR] COG0477 Permeases of the major facilitator superf... 248 2e-65 PA0246 [GEPR] COG0477 Permeases of the major facilitator superfa... 219 1e-56 BS_mdr [GEPR] COG0477 Permeases of the major facilitator superfa... 196 6e-50 VC1597 [GEPR] COG0477 Permeases of the major facilitator superfa... 190 5e-48 >DR0258 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 544 Score = 285 bits (730), Expect = 9e-77, Method: Compositional matrix adjust. Identities = 178/543 (32%), Positives = 278/543 (51%), Gaps = 25/543 (4%) Query: 4 LTQKKKVTXXXXXXXXXXXXXXNQTIVGTAMPRIIAMLGGIEHYTWVITIYMLTSTIATV 63 L++++K+ N T+VG+AMPR+I+ LGG Y W T Y LT+T+ Sbjct: 15 LSEREKLLAFAGILTVLFLSSLNMTVVGSAMPRVISDLGGFHLYAWAFTAYSLTTTVTIP 74 Query: 64 LVGKLSDIYGRKPFLLAGIIIFMIGAFLSGLSANIFQMIAYRGIQGVGGGILMSATVTAV 123 +VG LSD YGR+P +L GI++F +G+ G ++ Q+IA R IQG+GGG LM+ + TA+ Sbjct: 75 IVGTLSDRYGRRPLILLGIVVFALGSMGLGFVQSMEQLIALRAIQGIGGGTLMAMSFTAI 134 Query: 124 GDLFAPRERAKWTGIMMAMFGFSSVIGPTLGGFMIDHMDWKWIFWIFLPLGLVAFVMIWR 183 DLF P ER ++ G A++G SSV+GP +GGF+ DH W+ +F++ LP L+A IWR Sbjct: 135 ADLFTPIERGRYQGYTGAVWGISSVVGPLVGGFLTDHFGWRSVFFLNLPFALLALFFIWR 194 Query: 184 MF---PKTTRAASESIDYPGSVFLSLSIVALLLGFSWAGTQYDWGSPQXXXXXXXXXXXX 240 F T + D G+ L+ ++ L L SW G Y W SP+ Sbjct: 195 FFRLPAPTPGSVKGGFDALGATLLAGAVTTLTLAVSWGGGTYAWDSPRILGLLGATLALG 254 Query: 241 XXXXXXERKAKSPVLPLSLFKNSIISLSMVIGFLMNAGMMGAMIYLPFFVQGVEGVSPTN 300 + + +L L L ++ I L+ + GFL++AGM A++YLP ++QGV G S + Sbjct: 255 AWYAAHSARQERAILDLGLLRDRGILLASLAGFLVSAGMYAAILYLPLYMQGVRGSSASG 314 Query: 301 SGFINMPMSLMMIVLSTLVGRWISKSGKYKRYALIG---MPFMVAAMLIMAFMNSIAMAV 357 SG P+ MI+ STL G+ +S++G+YKR + G + ++ + +AV Sbjct: 315 SGLALAPLMGGMILTSTLSGQLVSRTGRYKRLIVGGALVASLALVLSSTLSLSTPLWLAV 374 Query: 358 ASMIIFGIGLGLAMPVFTLTVQNAVAPAMLGVATATSTLFRNLGGTIGIAIMGTVMNTTL 417 MI+ G+GLG TL VQNA LG AT + FR +GGT+ +++ G ++N L Sbjct: 375 TMMILLGLGLGPVNSQLTLAVQNAAPRQKLGSATGGNQFFRQIGGTLAVSLFGALVNAHL 434 Query: 418 THKLENAVHSGQGPDMSKLDAESARQLSAFM-----NPQMLLDQPKLKELHATLPEQ-IQ 471 +H L +KL A + R+L A M NP +L + L L + Sbjct: 435 SHDLS-----------AKLPA-AVRRLPAPMQDAIANPNLLTSERATHLLGWALKKMGHH 482 Query: 472 PLFNQLIEMLRSALSDSLTVVFLSGTXXXXXXXXXXXXXKEIPLRSSEARTEAKTGEKGV 531 + +++ LR + ++ VFL E PL+ A++ + E+ V Sbjct: 483 EMLAPIMQALRGVMVGAIDQVFLVSAGLVGLAFVLTLALPERPLQ-GRAQSRPQVAEEAV 541 Query: 532 DSK 534 + Sbjct: 542 ATD 544 >lin0980 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 491 Score = 248 bits (633), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 153/443 (34%), Positives = 251/443 (56%), Gaps = 10/443 (2%) Query: 28 TIVGTAMPRIIAMLGGIEHYTWVITIYMLTSTIATVLVGKLSDIYGRKPFLLAGIIIFMI 87 TIV TAMP I++ L GIE W+ ++Y+LTS + + GKLSD+YGRK + IIF+I Sbjct: 25 TIVSTAMPTIVSQLDGIELMNWIFSVYLLTSAVTVPIYGKLSDLYGRKNIFVIATIIFII 84 Query: 88 GAFLSGLSANIFQMIAYRGIQGVGGGILMSATVTAVGDLFAPRERAKWTGIMMAMFGFSS 147 G+ L G + N+ Q+I +R IQG+G G ++ +T+T + D++ +RAK G M + +G + Sbjct: 85 GSSLCGFAQNMEQLIIFRAIQGIGAGGILPSTMTIIADVYPFEKRAKVLGFMGSAWGIAG 144 Query: 148 VIGPTLGGFMIDHMDWKWIFWIFLPLGLVAFVMIWRMFPKTTRAASESIDYPGSVFLSLS 207 V GP +GGF++D + W WIF+I +P+GL+ ++I + IDY G+ +++ Sbjct: 145 VFGPLVGGFLVDQLSWHWIFFINVPIGLLTILLILLYLREEVERVKLPIDYLGASLFTVA 204 Query: 208 IVALLLGFSWAGTQYDWGSPQXXXXXXXXXXXXXXXXXXERKAKSPVLPLSLFKNSIISL 267 ++ LL AG +W P E++AK P++P LFKN ++ + Sbjct: 205 LLGLLFALQRAGESLNWTEPLVVILFAVSILLFVAFYFVEKRAKDPIMPFVLFKNPVVLI 264 Query: 268 SMVIGFLMNAGMMGAMIYLPFFVQGVEGVSPTNSGFINMPMSLMMIVLSTLVGRWISKSG 327 +IGFL++A ++G +Y+P + QG+ G T +GF+ P+S+ I+ S + G+ + G Sbjct: 265 GNLIGFLISAFLIGINVYIPMWAQGMLGHGATIAGFMLAPLSVTWIIGSFIGGKLLMTLG 324 Query: 328 KYKRYALIGMPFMVAAMLIMAFM----NSIAMAVASMIIFGIGLGLAMPVFTLTVQNAVA 383 + IG+ A+ LI+A N I + S + G G G+ T+TVQ+AV Sbjct: 325 N-RHTVGIGVLITAASGLILALFPASTNDIFFYLNSAFM-GFGFGIIFTTTTVTVQDAVP 382 Query: 384 PAMLGVATATSTLFRNLGGTIGIAIMGTVMNTTLTHKLENAVHSGQGPDMSKLDAESARQ 443 G+ATA++TLFR +G TIG+A+ GT+ N+ LT + A +G + S L+ + Q Sbjct: 383 RFQTGIATASNTLFRTVGQTIGVAVFGTIFNSVLTTQFRAA--AGSEVNRSNLNQLISPQ 440 Query: 444 LSAFMNPQMLLDQPKLKELHATL 466 S +NP ++ +P L++ L Sbjct: 441 TSGNVNPDLV--EPLRDILYSGL 461 >PA0246 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 501 Score = 219 bits (557), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 128/424 (30%), Positives = 221/424 (52%), Gaps = 12/424 (2%) Query: 26 NQTIVGTAMPRIIAMLGGIEHYTWVITIYMLTSTIATVLVGKLSDIYGRKPFLLAGIIIF 85 +QTIV ++P I A ++ WVI+ YM+ T++ + GKL D+YGR+ +L I++F Sbjct: 28 DQTIVAVSLPAISADYADLDLLAWVISAYMVAMTVSMPIYGKLGDLYGRRRLMLFAIVVF 87 Query: 86 MIGAFLSGLSANIFQMIAYRGIQGVGGGILMSATVTAVGDLFAPRERAKWTGIMMAMFGF 145 + L GL+ ++ Q++ R +QG+G G LM+ + +GD+ PRER ++ G +M+ Sbjct: 88 TAASLLCGLAQSMGQLVLARVLQGIGAGGLMAVSQAIIGDIVPPRERGRYQGYFSSMYAI 147 Query: 146 SSVIGPTLGGFMIDHMDWKWIFWIFLPLGLVAFVMIWRM---FPKTTRAASESIDYPGSV 202 +SV GP LGG + +++ W W+FWI LP+GL+A + R P R IDY G V Sbjct: 148 ASVAGPVLGGLLTEYLSWHWVFWINLPIGLLALAISRRTLVGLPVPRR--QPVIDYLGIV 205 Query: 203 FLSLSIVALLLGFSWAGTQYDWGSPQXXXXXXXXXXXXXXXXXXERKAKSPVLPLSLFKN 262 + + + ALLL + G W P ER+ + P++P+ LF Sbjct: 206 LMVVGLTALLLAITQIGQGAAWSDPALLGLFALAALALLLFVAQERRFREPLVPMQLFAV 265 Query: 263 SIISLSMVIGFLMNAGMMGAMIYLPFFVQGVEGVSPTNSGFINMPMSLMMIVLSTLVGRW 322 +LS ++ F + + + +P Q V G ++ +P+ + + + + GR Sbjct: 266 RAATLSWLVAFFASFQAISLTVLVPLRYQSVTGAGADSAALHLLPLVMGLPLGAYCCGRL 325 Query: 323 ISKSGKYKRYALIG---MPFMVAAMLIMAFMNSIAMAVASMIIFGIGLGLAMPVFTLTVQ 379 + G+YK L+G +P +A + ++ + ++ M++ GI +G P + Q Sbjct: 326 TASLGRYKPLILLGAVLLPCGIAGLALVPVGDHW-LSGLCMLLTGIAIGTQFPTSLVAAQ 384 Query: 380 NAVAPAMLGVATATSTLFRNLGGTIGIAIMGTVMNTTLTH-KLENAVHSG--QGPDMSKL 436 NAV +GVAT+ +TLFR+LGG +G+A+M ++ L H ++ + G + +S L Sbjct: 385 NAVEQRHIGVATSNATLFRSLGGAVGVALMSAILLALLKHLRIGGGLDQGFSESALLSSL 444 Query: 437 DAES 440 DA S Sbjct: 445 DAAS 448 >BS_mdr [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 426 Score = 196 bits (499), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 118/385 (30%), Positives = 193/385 (50%), Gaps = 5/385 (1%) Query: 26 NQTIVGTAMPRIIAMLGGIEHYTWVITIYMLTSTIATVLVGKLSDIYGRKPFLLAGIIIF 85 + TIV TAM I+A LG + + WV YM+ + GKLSD+YGRK F + Sbjct: 28 DNTIVATAMGNIVADLGSFDKFAWVTASYMVAVMAGMPIYGKLSDMYGRKRFSCLDLFFS 87 Query: 86 MIGAFLSGLSANIFQMIAYRGIQGVGGGILMSATVTAVGDLFAPRERAKWTGIMMAMFGF 145 +IG+ L G++ + Q+I +R IQG+GGG L+ T + DLF P +R K +G+ A+FG Sbjct: 88 LIGSALCGIAQTMNQLIIFRAIQGIGGGALLPIAFTIIFDLFPPEKRGKMSGMFGAVFGL 147 Query: 146 SSVIGPTLGGFMIDHMDWKWIFWIFLPLGLVAFVMIWRMFPKTTRAASESIDYPGSVFLS 205 SSV+GP LG + D + W W+F+I +P+G ++ I R + ++ + ID+ G++ L Sbjct: 148 SSVLGPLLGAIITDSISWHWVFYINVPIGALSLFFIIRYYKESLEHRKQKIDWGGAITLV 207 Query: 206 LSIVALLLGFSWAGTQYDWGSPQXXXXXXXXXXXXXXXXXXERKAKSPV-LPLSLFKNSI 264 +SIV L+ G YDW S Q E+KA+ P + + Sbjct: 208 VSIVCLMFALELGGKTYDWNSIQIIGLFIVFAVFFIAFFIVEKKAEDPARYSARSSRMAC 267 Query: 265 ISLSMVIGFLMNAGMMGAMIYLPFFVQGVEGVSPTNSGFINMPMSLMMIVLSTLVGRWIS 324 + ++ FL + +++P FVQ V S ++GFI PM ++ V+ +++G Sbjct: 268 LRQRQILAFLYEGIFIILAVFIPTFVQPVYASSAASAGFILTPM-MIGSVIRSMIGGIFQ 326 Query: 325 KSGKYKRYALIGMPFMVAAMLIMAFMNSIAMAV---ASMIIFGIGLGLAMPVFTLTVQNA 381 ++ LI + ML+++ M V M+I G G+G + + N Sbjct: 327 TKASFRNLMLISVIAFFIGMLLLSNMTPDTARVWLTVFMMISGFGVGFNFSLLSAASMND 386 Query: 382 VAPAMLGVATATSTLFRNLGGTIGI 406 + P G A +T++ R+ G T+G Sbjct: 387 LEPRFRGTANSTNSFLRSFGMTLGC 411 >VC1597 [GEPR] COG0477 Permeases of the major facilitator superfamily Length = 477 Score = 190 bits (483), Expect = 5e-48, Method: Compositional matrix adjust. Identities = 122/395 (30%), Positives = 206/395 (52%), Gaps = 4/395 (1%) Query: 26 NQTIVGTAMPRIIAMLGGIEHYTWVITIYMLTSTIATVLVGKLSDIYGRKPFLLAGIIIF 85 +QT++ TA P I+ LG ++ +W+ YML S + + G L D YGRKP L+ + IF Sbjct: 27 DQTLLATATPAIVRDLGNLQLSSWIAIGYMLASAASVPVYGWLGDQYGRKPMLMIALSIF 86 Query: 86 MIGAFLSGLSANIFQMIAYRGIQGVGGGILMSATVTAVGDLFAPRERAKWTGIMMAMFGF 145 +G+ + + N+ ++A R +QG+G G LMS + +G+L PRERA++ G ++F Sbjct: 87 ALGSVICAFAMNMPWLVAGRIVQGLGSGGLMSLSQALIGELVPPRERARFQGYFSSLFAV 146 Query: 146 SSVIGPTLGGFMIDHMDWKWIFWIFLPLGLVAFVMIWRM-FPKTTRAASESIDYPGSVFL 204 +S+ GP LGG ++ ++ W+W+FW LPL +A + R F +++R + SID G + Sbjct: 147 ASIGGPVLGGVLVTYLSWQWLFWFNLPLVGIALYRLARFQFVQSSRKSEASIDVAGLILF 206 Query: 205 SLSIVALLLGFSWAGTQYDWGSPQXXXXXXXXXXXXXXXXXXERKAKSPVLPLSLFKNSI 264 + ++ S G + W S +++ + LPL L Sbjct: 207 PSMMTLIIYWLSAGGHYFPWFSVTSISLVSAFVLTSLLFIAQQKRVTASFLPLRLLARRE 266 Query: 265 ISLSMVIGFLMNAGMMGAMIYLPFFVQGVEGVSPTNSGFINMPMSLMMIVLSTLVGRWIS 324 I + ++ F+ A + + +LP F+Q V+ SG + +P+S +I + G+ I+ Sbjct: 267 IHIPLLSTFIFAACLFALIFFLPIFLQLGLRVNAAQSGLLLIPLSCGIIGGAYTTGKVIA 326 Query: 325 KSGKYKRYALIGMPFMVAAMLIMAFMNSIAMAVASMI--IFGIGLGLAMPVFTLTVQNAV 382 K+G K + GM A ++A M S++ V S I + G+GLG MP + VQ Sbjct: 327 KTGVPKFLPVFGMSLSSCAFAVLA-MFSLSAPVVSAIAALCGLGLGTVMPSTQVLVQTLA 385 Query: 383 APAMLGVATATSTLFRNLGGTIGIAIMGTVMNTTL 417 A LG TA ++L R+LG ++G A GT++ + L Sbjct: 386 GKANLGRITAMASLSRSLGASVGTAFFGTLIYSLL 420 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.324 0.137 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 33,901,411 Number of extensions: 1251807 Number of successful extensions: 5108 Number of sequences better than 1.0e-05: 134 Number of HSP's gapped: 4991 Number of HSP's successfully gapped: 139 Length of query: 543 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 432 Effective length of database: 25,576,357 Effective search space: 11048986224 Effective search space used: 11048986224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 118 (50.1 bits)