BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0405.0004.p (297 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value STM1028 [R] COG3772 Phage-related lysozyme (muraminidase) 206 5e-53 TM1028 [V] COG1131 ABC-type multidrug transport system, ATPase c... 206 5e-53 Rv1218c [V] COG1131 ABC-type multidrug transport system, ATPase ... 194 2e-49 MT1256 [V] COG1131 ABC-type multidrug transport system, ATPase c... 191 1e-48 L192192 [V] COG1131 ABC-type multidrug transport system, ATPase ... 190 2e-48 >STM1028 [R] COG3772 Phage-related lysozyme (muraminidase) Length = 150 Score = 206 bits (523), Expect = 5e-53, Method: Compositional matrix adjust. Identities = 0/293 (0%), Positives = 0/293 (0%), Gaps = 2/293 (0%) Query: 1 MLKVEGLSKRFSNGRGIDNVSFTVHQGEVFGFLGPNGAGKSTTIRHIMGFMQPDRGSVTI 60 + +G++ G +G G M + + + Sbjct: 3 RISEKGITLIKEFEGCSLTAYPDPGTGGDPWTIGYGWTHSVDGKPVKPGMMIDEATAERL 62 Query: 61 GGLDAWKGQGTVQKLTGYLPGEINFIDGMTGTSFLNFMTSMQGMKDTSKRDRLIQRLQFD 120 + V +L + F ++ L T + Sbjct: 63 LNTGLVGYENDVSRLVKVKLTQGQFDALVSFAYNLGARTLSSSTLLRKLNAGDYAGAADE 122 Query: 121 AATPIRKMSKGMKQKVGIVAAFMHSPKLIILDEPTSGLDPLMQRVFIELVLEEKAAGTTF 180 Sbjct: 123 182 Query: 181 LMSSHSFQEIERTCDRAAIIKDGRIVTVKNIHELQSMQRKLFXXXXXXXXXXXXXXXSGL 240 Sbjct: 183 -- 240 Query: 241 QVESHEGNRVRIAIQGDYAAFTQELAHYAVRSIDISTQNLEDIFMNYYDRDRE 293 Sbjct: 241 293 >TM1028 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 293 Score = 206 bits (523), Expect = 5e-53, Method: Compositional matrix adjust. Identities = 108/293 (36%), Positives = 158/293 (53%), Gaps = 2/293 (0%) Query: 1 MLKVEGLSKRFSNGRGIDNVSFTVHQGEVFGFLGPNGAGKSTTIRHIMGFMQPDRGSVTI 60 +++V L+K + RG++ V+F+V +GE+FGF+GPNGAGK+TTIR ++G + PD G I Sbjct: 3 VVEVRDLTKYYGKSRGVEGVTFSVEEGEIFGFIGPNGAGKTTTIRLLLGLIFPDSGLAEI 62 Query: 61 GGLDAWKGQGTVQKLTGYLPGEINFIDGMTGTSFLNFMTSMQGMKDTSKRDRLIQRLQFD 120 G D K ++K GY+PGE++F +T FL + S D L D Sbjct: 63 FGKDVLKEGKEIRKNVGYIPGEVSFYPEVTVEEFLRYSASFYEKVDWDYVKELCGVFSLD 122 Query: 121 AATPIRKMSKGMKQKVGIVAAFMHSPKLIILDEPTSGLDPLMQRVFIELVLEEKAAGTTF 180 I+++S G K+KV IV A MH PKL+ILDEPT+GLDP++Q F E++ EK GTT Sbjct: 123 TKKRIKELSMGNKKKVAIVQALMHRPKLLILDEPTNGLDPIVQNTFFEILKNEKEKGTTV 182 Query: 181 LMSSHSFQEIERTCDRAAIIKDGRIVTVKNIHELQSMQRKLFXXXXXXXXXXXXXXXSGL 240 SSH E+ER CDR A+IKDGRI+ V+ + L+ + K+ + + Sbjct: 183 FFSSHILSEVERLCDRVAMIKDGRIIRVEKVENLKGERYKV--VRVKGENLEKLKDLAEV 240 Query: 241 QVESHEGNRVRIAIQGDYAAFTQELAHYAVRSIDISTQNLEDIFMNYYDRDRE 293 E G+ + L + + +LE+IFM+YY E Sbjct: 241 NRLKFENGTAEFLFSGNVERLLEILRSLKLSDFWVEEPSLEEIFMSYYREGEE 293 >Rv1218c [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 311 Score = 194 bits (492), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 104/297 (35%), Positives = 160/297 (53%), Gaps = 2/297 (0%) Query: 2 LKVEGLSKRFSNGRGIDNVSFTVHQGEVFGFLGPNGAGKSTTIRHIMGFMQPDRGSVTIG 61 +++ GL+K F + R +D + TV +GEV GFLGPNGAGKSTT+R ++G ++ D GSV + Sbjct: 10 IEIRGLTKHFGSVRALDGLDLTVREGEVHGFLGPNGAGKSTTLRILLGLVKADGGSVRLL 69 Query: 62 GLDAWKGQGTVQKLTGYLPGEINFIDGMTGTSFLNFMTSMQGMKDTSKRDRLIQRLQFDA 121 G D W + + Y+PG++ +TG ++ + M+G D ++R LI+R D Sbjct: 70 GGDPWTDAVDLHRHIAYVPGDVTLWPSLTGGETIDLLARMRGGIDNARRAELIERFGLDP 129 Query: 122 ATPIRKMSKGMKQKVGIVAAFMHSPKLIILDEPTSGLDPLMQRVFIELVLEEKAAGTTFL 181 R SKG +QKV +++A L++LDEP+SGLDPLM+ VF + + E + G T L Sbjct: 130 TKKARTYSKGNRQKVSLISALSSHATLLLLDEPSSGLDPLMENVFQQCIGEARQRGVTVL 189 Query: 182 MSSHSFQEIERTCDRAAIIKDGRIVTVKNIHELQSMQRKLFXXXXXXXXXXXXXXXSGLQ 241 +SSH E E C++ II+ G+ V ++ L+ + R G++ Sbjct: 190 LSSHILAETEALCEKVTIIRAGKTVESGSLDALRHLSRTSI-KAEMIGDPGDLSQIKGVE 248 Query: 242 VESHEGNRVRIAIQGD-YAAFTQELAHYAVRSIDISTQNLEDIFMNYYDRDREVTAE 297 S EG VR + + Q L H VRS+ LE++F+ +Y EV AE Sbjct: 249 DISIEGTTVRAQVDSESLRELIQVLGHAGVRSLVSQPPTLEELFLRHYSLGPEVAAE 305 >MT1256 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 311 Score = 191 bits (485), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 103/297 (34%), Positives = 159/297 (53%), Gaps = 2/297 (0%) Query: 2 LKVEGLSKRFSNGRGIDNVSFTVHQGEVFGFLGPNGAGKSTTIRHIMGFMQPDRGSVTIG 61 +++ GL+K F + R +D + TV +GEV GFLGPNGAGKSTT+R ++G ++ D GSV + Sbjct: 10 IEIRGLTKHFGSVRALDGLDLTVREGEVHGFLGPNGAGKSTTLRILLGLVKADGGSVRLL 69 Query: 62 GLDAWKGQGTVQKLTGYLPGEINFIDGMTGTSFLNFMTSMQGMKDTSKRDRLIQRLQFDA 121 G D W + + Y+PG++ +TG ++ + M+G D ++R LI+R D Sbjct: 70 GGDPWTDAVDLHRHIAYVPGDVTLWPSLTGGETIDLLARMRGGIDNARRAELIERFGLDP 129 Query: 122 ATPIRKMSKGMKQKVGIVAAFMHSPKLIILDEPTSGLDPLMQRVFIELVLEEKAAGTTFL 181 R SKG +QKV +++A L++LDEP+SGLDPLM+ VF + + E + G T L Sbjct: 130 TKKARTYSKGNRQKVSLISALSSHATLLLLDEPSSGLDPLMENVFQQCIGEARQRGVTVL 189 Query: 182 MSSHSFQEIERTCDRAAIIKDGRIVTVKNIHELQSMQRKLFXXXXXXXXXXXXXXXSGLQ 241 +SSH E E C++ II+ G+ ++ L+ + R G++ Sbjct: 190 LSSHILAETEALCEKVTIIRAGKTGESGSLDALRHLSRTSI-KAEMIGDPGDLSRIKGVE 248 Query: 242 VESHEGNRVRIAIQGD-YAAFTQELAHYAVRSIDISTQNLEDIFMNYYDRDREVTAE 297 S EG VR + + Q L H VRS+ LE++F+ +Y EV AE Sbjct: 249 DISIEGTTVRAQVDSESLRELIQVLGHAGVRSLVSQPPTLEELFLRHYSLGPEVAAE 305 >L192192 [V] COG1131 ABC-type multidrug transport system, ATPase component Length = 292 Score = 190 bits (483), Expect = 2e-48, Method: Compositional matrix adjust. Identities = 100/293 (34%), Positives = 158/293 (53%), Gaps = 10/293 (3%) Query: 1 MLKVEGLSKRFSNGRGIDNVSFTVHQGEVFGFLGPNGAGKSTTIRHIMGFMQPDRGSVTI 60 +++V+GL K F + + +VSF V+ GEV G++GPNGAGKS TIR ++G ++ +G V I Sbjct: 4 LVEVKGLQKNFGQFQALKDVSFEVNAGEVLGYIGPNGAGKSATIRILLGIIRATQGKVQI 63 Query: 61 GGLDAWKGQGTVQKLTGYLPGEINFIDGMTGTSFLNFMTSMQGMKDTSKRDRLIQRLQFD 120 G D W+ + K Y+P ++ ++G ++ + G + KRD+LI++ + Sbjct: 64 FGKDVWQDSIEIHKKIAYVPRDVYLWPNLSGGETIDLFLKLHGASNQEKRDQLIKKFDLN 123 Query: 121 AATPIRKMSKGMKQKVGIVAAFMHSPKLIILDEPTSGLDPLMQRVFIELVLEEKAAGTTF 180 +R SKG +QK+ ++AA +L I DEPTSGLDPLM+ VF E V KAAG Sbjct: 124 PKKKVRSYSKGNRQKIALIAALATEAELYIFDEPTSGLDPLMEAVFQEEVKALKAAGKAI 183 Query: 181 LMSSHSFQEIERTCDRAAIIKDGRIVTVKNIHELQSMQRKLFXXXXXXXXXXXXXXXS-- 238 ++SSH E+E+ DR A+I+ G IV + +L+ + R + S Sbjct: 184 ILSSHILSEVEKLADRVAVIRKGEIVETGTLDDLRHLTRYQYKVETEQEAVGLKELSSVH 243 Query: 239 GLQVESHEGNRVRIAIQGDYAAFTQ---ELAHYAVRSIDISTQNLEDIFMNYY 288 LQ++ +E Q D A + L Y V+ ++ + LED+FM +Y Sbjct: 244 DLQIKENEAT-----FQADSDAIDEILNTLLLYGVKKLEATPPTLEDLFMRHY 291 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.321 0.136 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 19,716,187 Number of extensions: 755968 Number of successful extensions: 8847 Number of sequences better than 1.0e-05: 1774 Number of HSP's gapped: 7096 Number of HSP's successfully gapped: 1945 Length of query: 297 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 191 Effective length of database: 26,153,027 Effective search space: 4995228157 Effective search space used: 4995228157 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 115 (48.9 bits)