BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0405.0002.p (401 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value CAC0933 [KG] COG1940 Transcriptional regulator/sugar kinase 128 1e-29 lin0217 [KG] COG1940 Transcriptional regulator/sugar kinase 122 9e-28 BH1094 [KG] COG1940 Transcriptional regulator/sugar kinase 112 8e-25 SP0668 [KG] COG1940 Transcriptional regulator/sugar kinase 95 2e-19 BH2758 [KG] COG1940 Transcriptional regulator/sugar kinase 91 3e-18 >CAC0933 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 375 Score = 128 bits (322), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 86/357 (24%), Positives = 167/357 (46%), Gaps = 23/357 (6%) Query: 10 NSRTKEMYLSVRKKGTVSKQTLLEESGLTVSTLTRIXXXXXXXXXXXEVGFGESTGGRRP 69 + R+K ++ ++KKG ++K+ +L+ + ++TL R+ E G +S GGR+P Sbjct: 6 DDRSKRIFDLLQKKGAMTKKEILKNIDMKLTTLNRLMRPLEDERLIVEYGIEDSAGGRKP 65 Query: 70 TLYETNPAYGYVFGLEISRTFSRXXXXXXXXXXXDEYSWPMDEQATPERLIESVHAQVLR 129 +Y N Y+ G++ISR F E + + +++ + + + + Sbjct: 66 IMYNVNLCGFYIIGIDISREFINIVFTNIKLELFYEDRINLKGILSEKKIADKIINCINK 125 Query: 130 MLEQASIDISQVIGLGIGAVGPLDRKEGMILQPAGFAAPGWSQVPVKERLEQLLDVPVCL 189 ++ + +D+ + G+G+ +L A F G+++ +K LE+ L V + Sbjct: 126 VVGELRLDVLTLFGVGVS-----------VLNVASFKCSGYTK--LKSELERELSSRVII 172 Query: 190 DNGANTALLGEYWAGGDRRQHQLLYLHAGIGLRSAIMNEGRILYGVIDTEGAVGQMIIEX 249 +NGAN+A L EY G +RR + Y + +RS ++ G++L + + E A M+I Sbjct: 173 ENGANSAALAEYLYGFNRRISNMAYFNCTEIIRSGVVLSGQVLRTINNKEDAFSNMLI-- 230 Query: 250 XXXXXXXXXXXXXAWESYVSIRTLEMQAREEWKRGRPTLLRELAGRPEDIEFAHLVEALQ 309 +YVS ++ + K GR T++++ +I+ L A+ Sbjct: 231 -----INNKNKFDFLGNYVSESSIINNFCSKVKSGRKTIIKK---NLREIKLLDLENAIN 282 Query: 310 AGDSVVNGIFGQAAAYCGIGLANLINILHPEEVILGGPLFSAADFFYREATRTALEK 366 GDS+ + +A GI LAN I +L + ++LGG L + + FY T+ ++ Sbjct: 283 KGDSLSKEVILNSAVIFGIALANYIQVLGLKAIVLGGSLIANSKTFYDVLTKIVFDR 339 >lin0217 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 404 Score = 122 bits (306), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 93/384 (24%), Positives = 162/384 (42%), Gaps = 21/384 (5%) Query: 20 VRKKGTVSKQTLLEESGLTVSTLTRIXXXXXXXXXXXEVGFGESTGGRRPTLYETNPAYG 79 +R+KG +++ + ++ +STLT I E STGGRR L N YG Sbjct: 24 IREKGEITRTEIAKKCDFGMSTLTYILDDLQQEGIILEGAETSSTGGRRAKLVRFNKDYG 83 Query: 80 YVFGLEISRTFSRXXXXXXXXXXXDEYSWPMDEQATPERLIESVHAQVLRMLEQASIDIS 139 +V +++ S P + PE I+ + V +M + D+ Sbjct: 84 FVVSVKVEEEQLLFALTDLNAEIIKNTSIPFSSEKKPEDAIDLIAENVKKMCKNR--DMK 141 Query: 140 QVIGLGIGAVGPLDRKEGMILQPAGFAAPGWSQVPVKERLE-QLLDVPVCLDNGANTALL 198 ++G+GI G ++RK+G +++ GW V ++ L D+PV +D N L Sbjct: 142 NLLGVGIAISGLVNRKKGTVIRSTML---GWENVALEAMLHVHFPDIPVYVDKNINCYTL 198 Query: 199 GEYWAGGDRRQHQLLYLHAGIGLRSAIMNEGRILYGVIDTEGAVGQMIIEXXXXXXXXXX 258 E W G ++ + + G GL +++ +I YG G G I+ Sbjct: 199 AELWLGEGKQSNNFATVSVGAGLGLSVVINRQIYYGAQGGAGEFGHTTIQPG-------- 250 Query: 259 XXXXAWESYVSIR-TLEMQAREEWKRGRPTLLRELAGRPE--DIEFAHLVEALQAGDSVV 315 ++ + + LEM A E + R R ++E E D F ++ ++ +AGD + Sbjct: 251 ----GYKCHCGQKGCLEMYASEFYFRNRGEEIKEAFPTSELNDFHFDNVAKSARAGDEMA 306 Query: 316 NGIFGQAAAYCGIGLANLINILHPEEVILGGPLFSAADFFYREATRTALEKTYYRGQYKV 375 + G+ Y G G+ N+IN +PE+VI+ G D F + A + + ++ Sbjct: 307 IELMGKMGEYLGYGIRNIINTFNPEKVIIVGEGLHHRDLFLTKIDEIASQNFFSGAGFET 366 Query: 376 RFTQGSLSDTXXXXXXXXLILNQL 399 T SL D L+++QL Sbjct: 367 EITTTSLEDPAWLQGAALLVIHQL 390 >BH1094 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 407 Score = 112 bits (281), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 88/349 (25%), Positives = 156/349 (44%), Gaps = 6/349 (1%) Query: 19 SVRKKGTVSKQTLLEESGLTVSTLTRIXXXXXXXXXXXEVGFGESTGGRRPTLYETNPAY 78 ++R G +S+ + +++ LT T+T I E GES GGR+P L + Sbjct: 21 TIRLDGPISRADIAKKTKLTPPTVTNIVAELIEEKLVRESKMGESRGGRKPILLTIDAKN 80 Query: 79 GYVFGLEISRTFSRXXXXXXXXXXXDEYSWPMDEQATPERLIESVHAQVLRMLEQASIDI 138 +V GL++ R + + + T E LI+ + + + EQ + Sbjct: 81 SFVIGLDVGGYRVRAVLTDLNGEVEGQTTVELSSDLTEETLIDQLIKITVTLFEQCKMQK 140 Query: 139 SQVIGLGIGAVGPLDRKEGMILQPAGFAAPGWSQVPVKERLEQLLDVPVCLDNGANTALL 198 +VIG+GIG G +D + GM + F + + +K E DVPV ++N A L Sbjct: 141 DKVIGIGIGMHGIVDHQSGMAVFAPNFQ---LTNIDLKTPFESTFDVPVFVENDARALAL 197 Query: 199 GEYWAGGDRRQHQLLYLHAGIGLRSAIMNEGRILYGVIDTEGAVGQMIIEXXXXXXXXXX 258 GE W G + ++ ++ G+G+ + I+ R+ +GV G +G ++ Sbjct: 198 GETWFGNGQGIDHVICVNVGVGIGAGIVLNKRLFHGVHGIAGEIGHTTVDIHGKKCTCGN 257 Query: 259 XXXXAWESYVSIRTLEMQAREEWKRGRPTLLRE-LAGRPEDIEFAHLVEALQAGDSVVNG 317 ++ L+ +A + GR T+L E + G + I + + AGD + Sbjct: 258 YG--CLQTVAGGNMLKERAIHDIASGRKTMLLEKVEGDMDRISGEIIHQCALAGDPLSIE 315 Query: 318 IFGQAAAYCGIGLANLINILHPEEVILGGPLFSAADFFYREATRTALEK 366 I +A Y GIG+ N+IN ++P+ +I+GG + A DF + E+ Sbjct: 316 ILEEAGKYLGIGITNIINFINPDRIIVGGGVSKAGDFVLEPLRKMVKER 364 >SP0668 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 320 Score = 95.1 bits (235), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 71/296 (23%), Positives = 139/296 (46%), Gaps = 18/296 (6%) Query: 104 DEYSWPMDEQATPERLIESVHAQVLRMLEQASIDISQVIGLGIGAVGPLDRKEGMILQPA 163 DE S +D+ +IES+ + L +L A+ D G+G+G+ G +DR +G ++ Sbjct: 39 DEGSHIVDD------MIESIQHR-LDLLGLAAADFQ---GIGMGSPGVVDRDKGTVI--- 85 Query: 164 GFAAPGWSQV-PVKERLEQLLDVPVCLDNGANTALLGEYWAGGDRRQHQLLYLHAGIGLR 222 G W + P+K+++E+ L +P +DN AN A LGE W G Q ++++ G G+ Sbjct: 86 GAYNLNWKTLQPIKQKIEKALGIPFFIDNDANVAALGERWMGAGDNQPDVVFITLGTGVG 145 Query: 223 SAIMNEGRILYGVIDTEGAVGQMIIEXXX--XXXXXXXXXXXAWESYVSIRTLEMQAREE 280 I+ EG++L+GV G +G + ++ S I L + +E Sbjct: 146 GGIVAEGKLLHGVAGAAGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADE 205 Query: 281 WKRGRPTLLRELAGRPEDIEFAHLVEALQAGDSVVNGIFGQAAAYCGIGLANLINILHPE 340 ++ L+ L E++ + + + GD + ++ + Y GI AN+ +IL+P Sbjct: 206 YE--GDAALKRLIDNGEEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPS 263 Query: 341 EVILGGPLFSAADFFYREATRTALEKTYYRGQYKVRFTQGSLSDTXXXXXXXXLIL 396 +++GG + +A +F + + E ++ + + + +L + L+L Sbjct: 264 TIVIGGGVSAAGEFLLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVL 319 >BH2758 [KG] COG1940 Transcriptional regulator/sugar kinase Length = 386 Score = 91.3 bits (225), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 81/366 (22%), Positives = 156/366 (42%), Gaps = 31/366 (8%) Query: 19 SVRKKGTVSKQTLLEESGLTVSTLTRIXXXXXXXXXXXEVGFGESTGGRRPTLYETNPAY 78 ++RK+ +S+ + +GL T++ + EVG GES+GGRRP + Sbjct: 21 TIRKEAPLSRAQVSLTTGLNKGTVSSLVAELIADNLVYEVGPGESSGGRRPVMLMFQNKA 80 Query: 79 GYVFGLEISRTFSRXXXXXXXXXXXDEYSWPMDEQATPERLIESVHAQVLRMLEQ----A 134 Y G+ I+ + ++ W + + + V+ Q+++++EQ A Sbjct: 81 AYAIGINIAVNYLSGVLTDLVGNVIEKVEWSLHVKE-----FDHVNQQMVQLIEQLMKAA 135 Query: 135 SIDISQVIGLGIGAVGPLDRKEGMILQPAGFAAPGWSQVPVKERLEQLLDVPVCLDNGAN 194 +IG+G+ G ++ + ++ P W ++P+K++LE+ +P+ ++N AN Sbjct: 136 PPSTYGIIGIGVAVPGIVNEEGDVLFAPN----LNWREIPLKKQLEEHFQLPIAVENEAN 191 Query: 195 TALLGEYWAGGDRRQHQLLYLHAGIGLRSAIMNEGRILYGVIDTEGAVGQMIIEXXXXXX 254 LGE G + +++ GIG+ S I+ + G G VG M IE Sbjct: 192 AGALGEKVFGKGKESENMIFFSIGIGIGSGIIFGNSLYRGKGGYSGEVGHMSIE--TNGP 249 Query: 255 XXXXXXXXAWESYVSIRTLEMQAREEWKRGRPTLLRELAGRPEDIEFAHLVEALQAGDSV 314 WE + S + + Q ++ E + L E + G+ Sbjct: 250 LCTCGNKGCWELFASEKAVLQQGEALYE--------------EVLTLDDLFEKARKGEQT 295 Query: 315 VNGIFGQAAAYCGIGLANLINILHPEEVILGGPLFSAADFFYREATRTALEK--TYYRGQ 372 +F + Y G G+AN+IN L+PE V++G + + +T ++ +++ Q Sbjct: 296 AIDLFQKIGVYLGTGIANMINALNPELVVVGNRMVKGRPWLEHSLKQTVADRALSFHAEQ 355 Query: 373 YKVRFT 378 V F+ Sbjct: 356 VLVEFS 361 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.137 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 27,265,150 Number of extensions: 1089623 Number of successful extensions: 1853 Number of sequences better than 1.0e-05: 61 Number of HSP's gapped: 1796 Number of HSP's successfully gapped: 61 Length of query: 401 Length of database: 38,378,431 Length adjustment: 109 Effective length of query: 292 Effective length of database: 25,807,025 Effective search space: 7535651300 Effective search space used: 7535651300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 117 (49.7 bits)