BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0402.0046.p (327 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin1805 [P] COG0053 Predicted Co/Zn/Cd cation transporters 261 2e-69 mll4860 [P] COG0053 Predicted Co/Zn/Cd cation transporters 84 3e-16 MTH1172_1 [P] COG0053 Predicted Co/Zn/Cd cation transporters 65 1e-10 MTH1893 [P] COG0053 Predicted Co/Zn/Cd cation transporters 64 3e-10 L67760 [P] COG0053 Predicted Co/Zn/Cd cation transporters 62 1e-09 >lin1805 [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 321 Score = 261 bits (666), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 140/317 (44%), Positives = 210/317 (66%), Gaps = 3/317 (0%) Query: 6 ESLGSLIRKGNKSSGSAAIGNTCIAVVKAVAFALTGSGSMFATMMHSIADAVNQLFVFTG 65 E LG L+++GNKS+ +AA+ NT ++++K V + TG+ +MFA +HS+ DA NQ FVF G Sbjct: 3 ELLG-LLKEGNKSALTAALVNTIVSIIKGVTYFFTGNIAMFAETLHSLGDAANQFFVFIG 61 Query: 66 SVLAEKRPTRRFPDGFGRVINLFCMVAVIVVTIMAYETMLEGFHLLKHPAEESGGYWINV 125 S L++KRPT+RFP GFGR++NL + AVIVV IMA+ET+ EGF + P S G+ IN+ Sbjct: 62 SALSKKRPTKRFPHGFGRMVNLVLLGAVIVVGIMAFETIREGFAHIIDPT-SSTGFLINL 120 Query: 126 IVLLLSIGADGFVWVKAMNEVLHESRIEAKGLRKFTASLKNVKRAAPPTRLVFYEDLVAT 185 +VLLL + V VKAM+E+ H+ +E+KGL F S+ N+ +A T+LVF ED VAT Sbjct: 121 VVLLLCTILEFSVLVKAMHEIAHDVGLESKGLSLFKDSILNLGKAKAATKLVFLEDSVAT 180 Query: 186 SGGALAMLAVLVTAFTSFKLLDGVSSILIAFMMIGVAFRVGYDNMVGLIGVAAPPEIEER 245 GG LAM+AV+++ +T F +G++S+LI MM V +V DN G+IG + + Sbjct: 181 GGGLLAMIAVIISHYTPFHQAEGIASMLIGVMMFIVVGKVFLDNAAGVIG-ESDQSMHLT 239 Query: 246 VARIIFDEPQVTDIYQMRILQEGRYYHVEGLIELKKGMTLADADDIKFKVRDKLLADPHI 305 V +++ +P V DI + +L+EG +HV+ +EL +TLA+ DDIK ++ + + + Sbjct: 240 VGQLVMSDPDVRDIQVLTVLKEGDVFHVDVEVELDPMLTLAEVDDIKDRLEENIGKLRGV 299 Query: 306 SDVTLGILEDNGIRNWE 322 +DV + ED+ IRNWE Sbjct: 300 ADVLISFDEDDAIRNWE 316 >mll4860 [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 317 Score = 84.0 bits (206), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 87/314 (27%), Positives = 144/314 (45%), Gaps = 15/314 (4%) Query: 15 GNKSSGSAAI-GNTCIAVVKAVAFALTGSGSMFATMMHSIADAVNQLFVFTGSVLAEKRP 73 G+K+ AA+ GN IA+ K A TGS +M + +HS+ D N + G A + Sbjct: 6 GSKTVIYAALAGNLAIALTKFAAAFFTGSSAMLSEGVHSLVDTGNGGLLLYGMHRAARPA 65 Query: 74 TRRFPDGFGRVINLFC-MVAVIVVTIMAYETMLEGFHLLKHPAEESGGYWINVIVLLLSI 132 R P G GR + + +VA++V + A ++ EG + P E +N IVL LS Sbjct: 66 DRTHPLGHGRELYFWSFIVALLVFALGAGVSLYEGIIHIIAP-EPVANVKVNYIVLGLSF 124 Query: 133 GADGFVWVKAMNEVLHESRIEAKGLRKFTASLKNVKRAAPPTRLVFYEDLVATSGGALAM 192 +G W+ A+ E E KG + + +L++ K P V +ED A G +A Sbjct: 125 LFEGSSWLVALKEFRRE-----KGRQSWLQALQSSKD--PSVYTVLFEDSAALLGLIVAF 177 Query: 193 LAVLVTAFTSFKLLDGVSSILIAFMMIGVAFRVGYDNMVGLIGVAAPPEIEERVARIIFD 252 +L LDG +SI IAF++ A + ++ LIG A PE++++V I+ Sbjct: 178 AGILAAELLEMPELDGAASIGIAFILGATAIFLARESKGLLIGEPASPEVQKKVLAIVQQ 237 Query: 253 EPQVTDIYQMRILQEGRYYHVEGL-IELKKGMTLADADDIKFKVRDKLLAD-PHISDVTL 310 + V + + G V GL IE + +T + + ++ +L + P IS + Sbjct: 238 DSAVQRANGVLTVHMGPREIVAGLSIEFEDHLTAPEIEACVERLEARLKKEMPEISRL-- 295 Query: 311 GILEDNGIRNWESQ 324 ++ WE + Sbjct: 296 -FVKPQTTGTWEQR 308 >MTH1172_1 [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 297 Score = 65.1 bits (157), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 66/294 (22%), Positives = 139/294 (47%), Gaps = 21/294 (7%) Query: 6 ESLGSLIRKGNKSSGSAAIGNTCIAVVKAVAFALTGSGSMFATMMHSIADAVNQLFVFTG 65 E+ G+ +++G +S + I N + V+K L+G+ ++ A +HS AD + VF G Sbjct: 3 ENDGAPLKEGETASKYSVIINMILVVIKGFTGYLSGNIALIADAIHSFADVFSSAAVFIG 62 Query: 66 SVLAEKRPTRRFPDGFGRVINLFCMVAVIVVTIMAYETMLEGFHLLKHPAEESGGYWINV 125 L++++P FP G+ R+ L ++ +++ I E + + +PA E ++ Sbjct: 63 LKLSQRKPDELFPYGYHRIETLVSLLVSVLIIITGLEITWDSIIYILNPASEVSMATASL 122 Query: 126 IVLLLSIGADGFVWVKAMNEVLHESRIEAKGLRKFTASLKNVKRAAPPTRLVFYEDLVAT 185 V L+SIG + A +V RI + TA L + K + Y D++++ Sbjct: 123 AVSLISIGVS---YAIAFYKVRVGRRIGS------TALLNDGKHS--------YVDVISS 165 Query: 186 SGGALAMLAVLVTAFTSFKLLDGVSSILIAFMMIGVAFRVGYDNMVGLIGVAAPPEIEER 245 L L +L + G++ ++I+ ++I + R+ +++ L+ + E Sbjct: 166 ---ILVFLGIL-GEYMGLHGFQGIAGVIISVVIIYIGIRLAKYDVLTLLDACIDRDSIEV 221 Query: 246 VARIIFDEPQVTDIYQMRILQEGRYYHVEGLIELKKGMTLADADDIKFKVRDKL 299 + + + P V ++ +RI + G Y E IEL++G+ + + I ++ ++ Sbjct: 222 IKKTVLSVPGVEGVHDIRIRRSGPYLFGELHIELERGLPVDKIEGIISQINSRV 275 >MTH1893 [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 268 Score = 64.3 bits (155), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 0/298 (0%), Positives = 0/298 (0%), Gaps = 37/298 (12%) Query: 12 IRKGNKSSGSAAIGNTCIAVVKAVAFALTGSGSMFATMMHSIADAVNQLFVFTGSVLAEK 71 S + + + +++ F+ + Sbjct: 7 SIFIPDSLTAETGDLKIKTYKVVLIARAASIFGVKRIVIYHDDADGEARFIRDILTYMDT 66 Query: 72 RPTRRFPDGFGRVINLFCMVAVIVVTIMAYETMLEGFHLL-------KHPAEESGGYWIN 124 R + + T Sbjct: 67 PQYLRRKVFPIMRELKHVGILPPLRTPHHPTGKPVTGEYRQGLTVKRVKKGTLVDIGADK 126 Query: 125 VIVLLLSIGADGFVWVKAMNEVLHESRIEAKGLRKFTASLKNVKRAAPPTRLVFYEDLVA 184 + + R+ + ++ + Sbjct: 127 LALCREK-------LTVNRIMSFRVVRLGKE------ILIEPDE--------PE------ 159 Query: 185 TSGGALAMLAVLVTAFTSFKLLDGVSSILIAFMMIGVAFRVGYDNMVGLIGVAAPPEIEE 244 VL T + L V + ++ + + V A Sbjct: 160 ---DRYWGYEVLDTRRNLAESLKTVGADVVVATSRNASPITSILDEVKTRMRGAREAAIL 216 Query: 245 RVARIIFDEPQVTDIYQMRILQEGRYYHVEGLIELKKGMTLADADDIKFKVRDKLLAD 302 Sbjct: 217 274 >L67760 [P] COG0053 Predicted Co/Zn/Cd cation transporters Length = 314 Score = 62.0 bits (149), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 76/300 (25%), Positives = 131/300 (43%), Gaps = 27/300 (9%) Query: 16 NKSSGS----AAIG-NTCIAVVKAVAFALTGSGSMFATMMHSIADAVNQLFVFTGSVLAE 70 KSSG AA+G N +A+ K + F L+GS +M +HS+ D NQ+ + G + Sbjct: 4 KKSSGMFAVFAALGANVLVAISKFIGFGLSGSAAMLNESIHSVVDCGNQILLLFGDRRSR 63 Query: 71 KRPTRRFPDGFGRVINLFCMVAVIVVTIMAYE----TMLEGFHLLKHPAEESGGYWINVI 126 + T G R F M IV T + + ++E + L HPA I + Sbjct: 64 QASTNLHQFGEARAKYFFSM---IVATFLFFGGGVIGVMEAYDKLVHPAHSVENAGIVIG 120 Query: 127 VLLLSIGADGFVWVKAMNEVLHESRIEAKGLRKFTASLKNVKRAAPPTRLVFYEDLVATS 186 +LL+ + +G A E+ E E + KF ++ ++F EDL A Sbjct: 121 ILLIGMAIEGSSLRVAFKEI-SELNTEKLPIFKFLHESRH-----SEILVIFAEDLCAII 174 Query: 187 GGALAMLAVLVTAFTSFKLLDGVSSILIAFMMIGVAFRVGYDNMVGLIGVAAPPEIEERV 246 G LA+L ++T T + D +S +LI F+++ A + + ++G E++ Sbjct: 175 GLLLALLGTVLTMVTGNPIFDAISGLLIGFLLMAAAIFLTREFYSLIVGERVTDSDLEKI 234 Query: 247 ARIIFDEPQVTDIYQMRILQEG-RYYHVEGLIELKKGMTLADAD-------DIKFKVRDK 298 FD P+V + ++ + G + I+L + L D + +I+ K+ DK Sbjct: 235 LT-AFDRPEVERVINVKTVHLGPTEILIAAKIDLVSKLVLEDYEIINQIEAEIRKKISDK 293 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.323 0.138 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,623,711 Number of extensions: 1017132 Number of successful extensions: 2366 Number of sequences better than 1.0e-05: 13 Number of HSP's gapped: 2356 Number of HSP's successfully gapped: 14 Length of query: 327 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 220 Effective length of database: 26,037,693 Effective search space: 5728292460 Effective search space used: 5728292460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 116 (49.3 bits)