BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0402.0039.p (312 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2932 [R] COG0673 Predicted dehydrogenases and related proteins 77 4e-14 TM0414 [R] COG0673 Predicted dehydrogenases and related proteins 70 4e-12 STM4425 [R] COG0673 Predicted dehydrogenases and related proteins 68 2e-11 SP1325 [R] COG0673 Predicted dehydrogenases and related proteins 68 2e-11 STM1133 [R] COG0673 Predicted dehydrogenases and related proteins 66 8e-11 >lin2932 [R] COG0673 Predicted dehydrogenases and related proteins Length = 333 Score = 77.0 bits (188), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 42/195 (21%), Positives = 90/195 (46%), Gaps = 5/195 (2%) Query: 1 MNIGILGTG-FGAYHASLLAKMEGVTRIVIFGRNEAKLGKLKEELGVXXXXXXXXXXXXX 59 + +G++G G G YHA + K+ V + I N+ + ++ E+ Sbjct: 5 IKVGVIGVGQMGQYHAEIYQKLPQVELVAICEFNDERRAEMAEKFQCKAYKDYHELLANT 64 Query: 60 XXXXXXXXXXSHLHSQYAIQALKAGKHVFCETPVCLSRADALALRQAEERYGQRIMVNQF 119 ++H +A+K KH+ E P+ D A+ + + Y + V Sbjct: 65 EIEAVIIVLPDNMHRDCVEEAVKHKKHILLEKPLAKELEDGKAMYELTKDYDKVFTVGFL 124 Query: 120 IKFDHAYTRLYESVNSGQYGKLLHVTLKRETAPLWGD---LGLSKIAANLMIHELDMVTW 176 ++FD + + + +++G+ G ++H+ +R +P+ G +G S ++ ++MIH++D + W Sbjct: 125 LRFDPRFNMIKQRLDNGELGDIIHLYCRR-NSPITGPKRYIGASDLSMHVMIHDIDYINW 183 Query: 177 LLGNPPISSIWGSSS 191 + + P+ I S S Sbjct: 184 YMDSEPVKVIAKSRS 198 >TM0414 [R] COG0673 Predicted dehydrogenases and related proteins Length = 334 Score = 70.1 bits (170), Expect = 4e-12, Method: Compositional matrix adjust. Identities = 58/204 (28%), Positives = 93/204 (45%), Gaps = 12/204 (5%) Query: 1 MNIGILGTG-FGAYHASLLAKMEGVTRIVIFGRNEAKLGKLKEELGVXXXXXXXXXXXXX 59 M IG++G G G HA L ++ I E +L ++KE+LGV Sbjct: 1 MRIGVIGLGRIGTIHAENLKMIDDAILYAISDVREDRLREMKEKLGVEKAYKDPHELIED 60 Query: 60 XXXXXXXXXXS-HLHSQYAIQALKAGKHVFCETPVCLSRADALALRQAEERYGQRIMVNQ 118 S + HS+ I KA KHVFCE P+ L+ AD + + E + I+ Sbjct: 61 PNVDAVLVCSSTNTHSELVIACAKAKKHVFCEKPLSLNLADVDRMIE-ETKKADVILFTG 119 Query: 119 F-IKFDHAYTRLYESVNSGQYGK--LLHVTLKRETAPLWGDLGLS-KIAANLMIHELDMV 174 F +FD + +L E+V +G GK +L +T + P + +S I ++ IH+ DM Sbjct: 120 FNRRFDRNFKKLKEAVENGTIGKPHVLRITSRDPAPPPLDYIRVSGGIFLDMTIHDFDMA 179 Query: 175 TWLLGNPPISSIWGSSSKDGQALV 198 +++G + ++ DG LV Sbjct: 180 RYIMGE-EVEEVFA----DGSVLV 198 >STM4425 [R] COG0673 Predicted dehydrogenases and related proteins Length = 336 Score = 68.2 bits (165), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 58/231 (25%), Positives = 101/231 (43%), Gaps = 17/231 (7%) Query: 1 MNIGILGTGF-GAYHASLLAK-MEGVTRI----VIFGRNEAKLGKLKEELGVXXXXXXXX 54 + GI+G G G+ H LA + GV + ++ GR +A L K E Sbjct: 3 LKAGIVGIGMIGSDHLRRLANTVSGVEVVAVCDIVAGRAQAALDKYAIE--AKDYNDYHD 60 Query: 55 XXXXXXXXXXXXXXXSHLHSQYAIQALKAGKHVFCETPVCLSRADALALRQAEERYGQRI 114 + H+ A+ AL A K+VFCE P+ ++ AD + +AE++ G+R+ Sbjct: 61 LINDKDVEVVIITASNEAHADVAVAALNANKYVFCEKPLAVTAADCQRVIEAEQKNGKRM 120 Query: 115 MVNQFI-KFDHAYTRLYESVNSGQYGKLLHVTLKRETAPLWGDLGLSKIAANLMIHELDM 173 + F+ ++D Y +L ++SG+ G+ L V + A + + +IHE+D+ Sbjct: 121 VQIGFMRRYDKGYVQLKNIIDSGEIGQPLMVHGRHYNASTVPEYKTPQAIYETLIHEIDV 180 Query: 174 VTWLLGNP--------PISSIWGSSSKDGQALVHADFDTPQFSAEILVSSQ 216 + WLL P S ++ +D Q +V E+ V+ Q Sbjct: 181 MHWLLNEDYKTVKVYFPRQSSLVTTLRDPQLVVMETTSGINIVVEVFVNCQ 231 >SP1325 [R] COG0673 Predicted dehydrogenases and related proteins Length = 379 Score = 67.8 bits (164), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 54/191 (28%), Positives = 84/191 (43%), Gaps = 12/191 (6%) Query: 1 MNIGILGTG-FGAYHASLLAKMEGVTRIVIFGRNEAKLGKLKEELGVXXXXXXXXXXXXX 59 +N GI+G G FGA A + K+E + +F N + ++ +ELG Sbjct: 12 VNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGE--EVAQELGSDVCASLDELVARE 69 Query: 60 XXXXXXXXXXSHLHSQYAIQALKAGKHVFCETPVCLSRADALALRQAEERYGQRIMVNQF 119 S+LH + ++A + GKHVFCE P+ LS D A+ A + M Sbjct: 70 DIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKAMVDACKENNVIFMAGHI 129 Query: 120 IKFDHAYTRLYESVNSGQYGKLLHVTLKR----ETAPL--WGDLGLSKIAANLM--IHEL 171 + F + E + G+ GK+L+ R E P W L S+ +L IHEL Sbjct: 130 MNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSWKKL-RSQSGGHLYHHIHEL 188 Query: 172 DMVTWLLGNPP 182 D + +++G P Sbjct: 189 DCIQFIMGGLP 199 >STM1133 [R] COG0673 Predicted dehydrogenases and related proteins Length = 368 Score = 65.9 bits (159), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 61/242 (25%), Positives = 98/242 (40%), Gaps = 21/242 (8%) Query: 4 GILGTG-FGAYHASLLAKMEGVTRIVIFGRNEAKLGKLKEELGVXXXXXXXXXXXXXXXX 62 G++GTG FGA A ++K+EG I+ + + +EL Sbjct: 6 GVVGTGYFGAELARFMSKVEGAKITAIY--DPVNAAPIAKELNCVATATMEALCSHPDVD 63 Query: 63 XXXXXXXSHLHSQYAIQALKAGKHVFCETPVCLSRADALALRQAEERYGQRIMVNQFIKF 122 ++LH I A KAGKHVFCE P+ L+ D + A + G M + F Sbjct: 64 CVIIASPNYLHKAPVIAAAKAGKHVFCEKPIALNYQDCKDMVDACKEAGVTFMAGHVMNF 123 Query: 123 DHAYTRLYESVNSGQYGKLLHVTLKRET-APLWGDLGLSKIAANL------MIHELDMVT 175 H + +G+ G++ V KR + ++ KI A IHELD Sbjct: 124 FHGVRHAKALIKAGEIGEVTQVHTKRNGFEDVQDEISWKKIRAKSGGHLYHHIHELDCTL 183 Query: 176 WLLGNPP--ISSIWGSSSKDGQ--------ALVHADFDTPQFSAEILVSS-QMPEAYPFT 224 +++ P +S G+ + G+ L+ +F++ +F+ SS PE Y Sbjct: 184 FIMDETPSLVSMAAGNVAHKGEKFGDEDDVVLITLEFESGRFATLQWGSSFHYPEHYVLI 243 Query: 225 VG 226 G Sbjct: 244 EG 245 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 20,949,943 Number of extensions: 785042 Number of successful extensions: 1640 Number of sequences better than 1.0e-05: 31 Number of HSP's gapped: 1629 Number of HSP's successfully gapped: 31 Length of query: 312 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 206 Effective length of database: 26,153,027 Effective search space: 5387523562 Effective search space used: 5387523562 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 116 (49.3 bits)