BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0402.0035.p (511 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2515 [T] COG0642 Signal transduction histidine kinase 126 1e-28 BH1154_2 [T] COG0642 Signal transduction histidine kinase 124 3e-28 lin2727 [T] COG0642 Signal transduction histidine kinase 120 5e-27 baeS [T] COG0642 Signal transduction histidine kinase 114 3e-25 ZbaeS [T] COG0642 Signal transduction histidine kinase 114 3e-25 >lin2515 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 126 bits (316), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 0/223 (0%), Positives = 0/223 (0%), Gaps = 7/223 (3%) Query: 285 DFLLNISHDLRTPLTSIMGFLSLLKEKSYDSEPEKERYIEVLEERTGYLKKLIDEFFEIS 344 Sbjct: 162 221 Query: 345 QLKWKPAVLNKSRVNMQELLRQVVEGYHPQSRDRRIETVLLLPDQSVHVEIDADLFVRVL 404 Sbjct: 222 281 Query: 405 ENLLSNAFKYSRADTELTIRLREPVAENRRQCLLRLEMWSTPAQAMENGELNRLFDRFYK 464 Sbjct: 282 ------- 334 Query: 465 GDSSRTGEGAGLGLSIAAEIIRLHGGRLDAQIEGERLGMMIEL 507 Sbjct: 335 377 >BH1154_2 [T] COG0642 Signal transduction histidine kinase Length = 274 Score = 124 bits (312), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 83/265 (31%), Positives = 144/265 (54%), Gaps = 21/265 (7%) Query: 254 IPLKYNNELSTMAAALNDMA--------EQIVVSRSKEKDFLLNISHDLRTPLTSIMGFL 305 +P++ + L+ +A +N M EQ R K + + N+SHDLRTPLTSI+ + Sbjct: 20 LPVRGRSALALLAQNINTMKKGMKQSEREQAKSERLK-TELITNVSHDLRTPLTSIITYT 78 Query: 306 SLLKEKSYDSEPEKERYIEVLEERTGYLKKLIDEFFEISQLKWKPAVLNKSRVNMQELLR 365 LLK+ E E+ YIE+++ + LK LID+ FE S++ L V++ +LL+ Sbjct: 79 ELLKDAD---EQERADYIEIIDRKARRLKILIDDLFEASKMSSGNIELTLETVDVLQLLQ 135 Query: 366 QVVEGYHPQSRDRRIETVLLLPDQSVHVEIDADLFVRVLENLLSNAFKYSRADTELTIRL 425 Q + Y ++ + +P++ ++++ D RV +NL+ N KYS T + + + Sbjct: 136 QALAEYGEALEQSSLQLRISVPEEPLYIKADGQKIWRVFDNLIQNILKYSLEHTRVYLSV 195 Query: 426 REPVAENRRQCLLRLEMWSTPAQAMENGE-LNRLFDRFYKGDSSRTGEGAGLGLSIAAEI 484 +E +++ +E+ E G+ ++ L +RF +GD SR EG+GLGL+IA I Sbjct: 196 KE-----KKET---VEIIIKNVAKYELGDNVDELLERFKRGDQSRNTEGSGLGLAIAKSI 247 Query: 485 IRLHGGRLDAQIEGERLGMMIELFM 509 + LH G LD ++G+ ++I+L M Sbjct: 248 VDLHEGYLDIDVDGDLFKVIIQLPM 272 >lin2727 [T] COG0642 Signal transduction histidine kinase Length = 182 Score = 120 bits (302), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 0/260 (0%), Positives = 0/260 (0%), Gaps = 12/260 (4%) Query: 238 LEQGTHTLYATDFEARIPLKYNNELSTMAAALNDMAEQIVVSRSKEKDFLLNISHDLRTP 297 Sbjct: 194 253 Query: 298 LTSIMGFLSLLKEKSYDSEPEKERYIEVLEERTGYLKKLIDEFFEISQLKWKPAVLNKSR 357 Sbjct: 254 - 312 Query: 358 VNMQELLRQVVEGYHPQSRDRRIETVLLLPDQSVHVEIDADLFVRVLENLLSNAFKYSRA 417 Sbjct: 313 -- 370 Query: 418 DTELTIRLREPVAENRRQCLLRLEMWSTPAQAMENGELNRLFDRFYKGDSSRTGE--GAG 475 Sbjct: 371 ------- 423 Query: 476 LGLSIAAEIIRLHGGRLDAQ 495 Sbjct: 424 443 >baeS [T] COG0642 Signal transduction histidine kinase Length = 467 Score = 114 bits (286), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 77/262 (29%), Positives = 131/262 (50%), Gaps = 13/262 (4%) Query: 235 MRTLEQGTHTLYATDFEARIPLKYNNELSTMAAALNDMAEQIVVSRSKEKDFLLNISHDL 294 ++ L GTH L A DF R+ +EL +A N +A + ++ +DF+ +ISH+L Sbjct: 193 VKRLVDGTHKLAAGDFTTRVTPTSEDELGKLAQDFNQLASTLEKNQQMRRDFMADISHEL 252 Query: 295 RTPLTSIMGFLSLLKEKSYDSEPEKERYIEVLEERTGYLKKLIDEFFEISQLKWKPAVLN 354 RTPL + G L +++ PE + L+ G L KL+D+ ++S Sbjct: 253 RTPLAVLRGELEAIQDGVRKFTPET---VASLQAEVGTLTKLVDDLHQLSMSDEGALAYQ 309 Query: 355 KSRVNMQELLRQVVEGYHPQSRDRRIETVLLLPDQSVHVEIDADLFVRVLENLLSNAFKY 414 K+ V++ LL + + R ++ LPD S+ V D D +++ NLL N+ +Y Sbjct: 310 KAPVDLIPLLEVAGGAFRERFASRGLKLQFSLPD-SITVFGDRDRLMQLFNNLLENSLRY 368 Query: 415 SRADTELTIRLREPVAENRRQCLLRLEMWSTPAQAMENGELNRLFDRFYK--GDSSRTGE 472 + + L I + +R +RL ++ A + + +L +LF+RFY+ G +R Sbjct: 369 TDSGGSLQI------SAGQRDKTVRL-TFADSAPGVSDDQLQKLFERFYRTEGSRNRASG 421 Query: 473 GAGLGLSIAAEIIRLHGGRLDA 494 G+GLGL+I I+ H GR+ A Sbjct: 422 GSGLGLAICLNIVEAHNGRIIA 443 >ZbaeS [T] COG0642 Signal transduction histidine kinase Length = 467 Score = 114 bits (286), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 76/262 (29%), Positives = 130/262 (49%), Gaps = 13/262 (4%) Query: 235 MRTLEQGTHTLYATDFEARIPLKYNNELSTMAAALNDMAEQIVVSRSKEKDFLLNISHDL 294 ++ L GTH L A DF R+ +EL +A N +A + ++ +DF+ +ISH+L Sbjct: 193 VKRLVDGTHKLAAGDFTTRVTPTSEDELGKLAQDFNQLASTLEKNQQMRRDFMADISHEL 252 Query: 295 RTPLTSIMGFLSLLKEKSYDSEPEKERYIEVLEERTGYLKKLIDEFFEISQLKWKPAVLN 354 RTPL + G L +++ PE + L+ G L KL+D+ ++S Sbjct: 253 RTPLAVLRGELEAIQDGVRKFTPET---VASLQAEVGTLTKLVDDLHQLSMSDEGALAYQ 309 Query: 355 KSRVNMQELLRQVVEGYHPQSRDRRIETVLLLPDQSVHVEIDADLFVRVLENLLSNAFKY 414 K+ V++ LL + + R ++ LPD S+ V D D +++ NLL N+ +Y Sbjct: 310 KAPVDLIPLLEVAGGAFRERFASRGLKLQFSLPD-SITVFGDRDRLMQLFNNLLENSLRY 368 Query: 415 SRADTELTIRLREPVAENRRQCLLRLEMWSTPAQAMENGELNRLFDRFYK--GDSSRTGE 472 + + L I + + +RL ++ A + + +L +LF+RFY+ G +R Sbjct: 369 TDSGGSLKI------SAEQHDKTVRL-TFADSAPGVSDDQLQKLFERFYRTEGSRNRASG 421 Query: 473 GAGLGLSIAAEIIRLHGGRLDA 494 G+GLGL+I I+ H GR+ A Sbjct: 422 GSGLGLAICLNIVEAHNGRIIA 443 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.135 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 35,133,078 Number of extensions: 1439199 Number of successful extensions: 3126 Number of sequences better than 1.0e-05: 196 Number of HSP's gapped: 2813 Number of HSP's successfully gapped: 196 Length of query: 511 Length of database: 38,378,431 Length adjustment: 111 Effective length of query: 400 Effective length of database: 25,576,357 Effective search space: 10230542800 Effective search space used: 10230542800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 118 (50.1 bits)