BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0402.0025.p (315 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value PAE3466 [H] COG0303 Molybdopterin biosynthesis enzyme 168 1e-41 AF0930 [H] COG0303 Molybdopterin biosynthesis enzyme 160 2e-39 PA3028 [H] COG0303 Molybdopterin biosynthesis enzyme 157 2e-38 RSc1037 [H] COG0303 Molybdopterin biosynthesis enzyme 152 8e-37 PAE3465_1 [H] COG0303 Molybdopterin biosynthesis enzyme 144 1e-34 >PAE3466 [H] COG0303 Molybdopterin biosynthesis enzyme Length = 211 Score = 168 bits (425), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 0/309 (0%), Positives = 0/309 (0%), Gaps = 6/309 (1%) Query: 2 TGAQVPAGADTVVMLEATESREEGGRLQVGIRKPQAAGKNVTPRGFELKEGELVLPAGRM 61 + A V + A ++ + Sbjct: 105 PEDRAALEALPGVGRKTANVVLN----TAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE 160 Query: 62 IKAGDIAVLAAFGIPLVQVIRRPRVGIFATGTELLDVHE-PLVPGKIRNSNSPMLEALVR 120 K + +I Sbjct: 161 EKLLKVVPAEFKVDCHHWLILH-GRYTCIARKPRCGSCII 219 Query: 121 ECGGEPVMLGAIVDDIEQARSKVQIALESYDVVITTGGVSVGDYDIMGDLIRENSGDMLF 180 Sbjct: 220 279 Query: 181 NKVTMRPGSVTTAAVRGGTLLLALSGNPGACFVGFHLFARPAILQMQGADQPFLPEWTAV 240 Sbjct: 280 339 Query: 241 LGADYNKVNNYTRFVRARLEIHEGQLHAYPAVIDESSVMVTIKDSDCLIVIPPEERGVSA 300 Sbjct: 340 399 Query: 301 GNSAKVLKL 309 Sbjct: 400 408 >AF0930 [H] COG0303 Molybdopterin biosynthesis enzyme Length = 414 Score = 160 bits (406), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 107/295 (36%), Positives = 167/295 (56%), Gaps = 8/295 (2%) Query: 1 MTGAQVPAGADTVVMLEATESREEGGRLQVGIRKPQAAGKNVTPRGFELKEGELVLPAGR 60 MTGA +P G + VVMLE T R G ++V K KNV+ G ++ GE+V G Sbjct: 106 MTGAMMPEGTNAVVMLEHT--RLNGNFVEV--LKSVTPMKNVSRVGEDIAAGEVVFRKGE 161 Query: 61 MIKAGDIAVLAAFGIPLVQVIRRPRVGIFATGTELLDVHEPLVPGKIRNSNSPMLEALVR 120 +++ D V+A+ GI V+V R+PRV + TG EL +V EPL KI NSN+PM+ + Sbjct: 162 ILQPQDAGVIASLGIESVRVYRKPRVAVITTGNELAEVGEPLEGAKIYNSNNPMICNALL 221 Query: 121 ECGGEPVMLGAIVDDIEQARSKVQIALESYDVVITTGGVSVGDYDIMGDLIRENSGDMLF 180 E G E + LG DD E+ R+K+Q ALE +D + TGG SVG D++ D++ E G+++F Sbjct: 222 EFGFEAISLGIARDDEEELRNKLQKALE-FDAAVITGGTSVGAKDLVPDVVGE-FGEIVF 279 Query: 181 NKVTMRPGSVTTAAVRGGTLLLALSGNPGACFVGFHLFARPAILQMQGADQPFLPEWTAV 240 + V+M+PG T AAV G + L G+P A +GF+ FA PA+ +M + +W+ Sbjct: 280 HGVSMKPGMPTGAAVVEGKPVFMLPGSPAAALLGFYTFAIPALYRMMNV-RIIARKWSRQ 338 Query: 241 LGADYNKVNNYTRFVRARLEIHEGQLHAYPAVIDESSVMVTIKDSDCLIVIPPEE 295 G ++ + VR+ + + YP I S ++ + ++ L+V+P ++ Sbjct: 339 KGVLQGRIPSEIG-VRSNVRVLWEDGKVYPIRISGSGILSSFVRANALLVVPEDK 392 >PA3028 [H] COG0303 Molybdopterin biosynthesis enzyme Length = 405 Score = 157 bits (397), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 96/272 (35%), Positives = 158/272 (58%), Gaps = 11/272 (4%) Query: 1 MTGAQVPAGADTVVMLEATESREEGGRLQVGIRKPQAAGKNVTPRGFELKEGELVLPAGR 60 TGA +P GAD V M E E+ ++G +V +P G++V PRG E+++G+LVLPAG Sbjct: 102 FTGAPLPEGADLVEMQENAEALDDG---RVRFTQPLRVGQHVRPRGQEIRQGDLVLPAGT 158 Query: 61 MIKAGDIAVLAAFGIPLVQVIRRPRVGIFATGTELLDVHEPLVPGKIRNSNSPMLEALVR 120 +++ ++ + A+ G ++V R+PRV + +TG EL++ +PL G+I NSN P+L+ ++ Sbjct: 159 RLRSIELGLAASLGYAALEVRRKPRVALLSTGDELVEAGQPLQAGQIYNSNRPLLKDWLQ 218 Query: 121 ECGGEPVMLGAIVDDIEQARSKVQIALESYDVVITTGGVSVGDYDIMGDLIRENSGDMLF 180 G + + G + DD+ + R ++ L D++++TGGVSVG+ D +G +RE +G++ Sbjct: 219 RLGCDVLDAGILPDDLARTRERL-AGLAGVDLILSTGGVSVGEADFLGMALRE-AGELTL 276 Query: 181 NKVTMRPGSVTTAAVRGGTLLLALSGNPGACFVGFHLFARPAILQMQGADQ--PFLPEWT 238 K+ ++PG T G ++ L GNP + V F L ARP +L+ G Q P E Sbjct: 277 WKLAIKPGKPLTVGQFQGIPVIGLPGNPASTLVTFALLARPYLLRRLGVAQVAPLRVEVP 336 Query: 239 AVLGADYNKVNNYTRFVRARLEIHEGQLHAYP 270 A G + K ++R RLE +G+ YP Sbjct: 337 A--GFAWTKAGQRREYLRGRLE--QGRAVLYP 364 >RSc1037 [H] COG0303 Molybdopterin biosynthesis enzyme Length = 429 Score = 152 bits (383), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 109/318 (34%), Positives = 164/318 (51%), Gaps = 9/318 (2%) Query: 1 MTGAQVPAGADTVVMLEATESREEGGRLQVGIRKPQAAGKNVTPRGFELKEGELVLPAGR 60 MTGA +PAG +TV+ E T+ R R AG N RG +L +G L AGR Sbjct: 116 MTGAVMPAGCNTVIPQEFTQGDAATVRFA---RDAVRAGDNRRLRGEDLAKGSAALTAGR 172 Query: 61 MIKAGDIAVLAAFGIPLVQVIRRPRVGIFATGTELLDVHEPLVPGKIRNSNSPMLEALVR 120 +++ DI +LA+ G+ V V RR RV F+TG EL + EPL G + +SN L ++ Sbjct: 173 VLRPADIGLLASLGVAEVPVRRRLRVAFFSTGDELRSIGEPLDAGCVYDSNRYTLHGMLS 232 Query: 121 ECGGEPVMLGAIVDDIEQARSKVQIALESYDVVITTGGVSVGDYDIMGDLIRENSGDMLF 180 G E + +G + DD + + A E+ D +IT+GGVSVG+ D ++ + GD+ F Sbjct: 233 HLGVELIDMGVVRDDPGAMEATFRSAAENADAIITSGGVSVGEADFTKQMMAQ-LGDVTF 291 Query: 181 NKVTMRPG---SVTTAAVRG-GTLLLALSGNPGACFVGFHLFARPAILQMQGADQPFLPE 236 K+ MRPG + A G +L L GNP A V F+ F R A+L+M GA + P Sbjct: 292 WKIAMRPGRPMAFGRIASNGRSAVLFGLPGNPVAVMVTFYHFVRGALLRMMGAAETGAPL 351 Query: 237 WTAVLGADYNKVNNYTRFVRARLEIH-EGQLHAYPAVIDESSVMVTIKDSDCLIVIPPEE 295 A A K T + R ++ GQL S V+ ++ +++C +V+ E+ Sbjct: 352 VPATSAAPIRKRPGRTEYQRGIAALNGSGQLEVRLTGQQGSGVLRSMSEANCFVVLAHEQ 411 Query: 296 RGVSAGNSAKVLKLPGEI 313 V+AG++ ++L G I Sbjct: 412 GPVNAGDTVQLLLFDGLI 429 >PAE3465_1 [H] COG0303 Molybdopterin biosynthesis enzyme Length = 415 Score = 144 bits (364), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 109/309 (35%), Positives = 158/309 (51%), Gaps = 14/309 (4%) Query: 2 TGAQVPAGADTVVMLEATESREEGGRLQVGIRKPQAAGKNVTPRGFELKEGELVLPAGRM 61 TGA +P GA+ VVM+E T++R G L V I + A G+NV G ++ GE+VL Sbjct: 107 TGAPLPRGANAVVMVEFTQTR---GDL-VKIFRAVAPGENVMSAGSDISAGEVVLRHCTK 162 Query: 62 IKAGDIAVLAAFGIPLVQVIRRPRVGIFATGTELLDVHEPLVPGKIRNSNSPMLEALVRE 121 + A +I VLAA G+ V V++RP VGI +TG EL E L PG + + NS L A V+E Sbjct: 163 LTAREIGVLAALGLRKVPVMKRPIVGIISTGNELAAPGERLGPGMLYDVNSYSLAAAVQE 222 Query: 122 CGGEPVMLGAIVDDIEQARSKVQIALESYDVVITTGGVSVG----DYDIMGDLIRENSGD 177 GG PV+ G + D+ +S + AL DVV+ +GG S G Y ++G+L GD Sbjct: 223 AGGLPVIYGIVRDEESSYKSALLKALSESDVVLISGGTSAGVADLTYRVLGEL-----GD 277 Query: 178 MLFNKVTMRPGSVTTAAVRGGTLLLALSGNPGACFVGFHLFARPAILQMQGADQPFLPEW 237 +LF+ V +RPG T AAV G +++ L G P + + FH RP +L++Q + + Sbjct: 278 VLFHGVMVRPGKPTLAAVINGKIVVGLPGYPSSALMIFHTVVRPFLLKLQCVEPSPPAVY 337 Query: 238 TAVLGADYNKVNNYTRFVRARLEIHEGQLHAYPAVIDESSVMVTIKDSDCLIVIPPEERG 297 A L L + AYP + ES + + +D IVIP Sbjct: 338 KAKLAYGVEGAKGRRALYPVVLIARKDGYKAYP-LYAESGAISVLARADGYIVIPENVEF 396 Query: 298 VSAGNSAKV 306 ++ G V Sbjct: 397 MTEGEEVDV 405 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.138 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 24,097,535 Number of extensions: 1048941 Number of successful extensions: 3260 Number of sequences better than 1.0e-05: 46 Number of HSP's gapped: 3152 Number of HSP's successfully gapped: 46 Length of query: 315 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 209 Effective length of database: 26,153,027 Effective search space: 5465982643 Effective search space used: 5465982643 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)