BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0402.0017.p (310 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value L182555 [F] COG0167 Dihydroorotate dehydrogenase 271 1e-72 SP0964 [F] COG0167 Dihydroorotate dehydrogenase 232 5e-61 STM2187_1 [F] COG0167 Dihydroorotate dehydrogenase 100 6e-21 ZyeiA_1 [F] COG0167 Dihydroorotate dehydrogenase 95 1e-19 >L182555 [F] COG0167 Dihydroorotate dehydrogenase Length = 311 Score = 271 bits (692), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 140/305 (45%), Positives = 195/305 (63%), Gaps = 11/305 (3%) Query: 4 MTANIAGVHFKNPVIMASGTFGFGREYSKLYDVSLLGGISGKGLTLHPKAGNPGVRVYET 63 ++ + G+ KNP+I ASG FGFG EY+K YD++ LG I K TLHP+ GNP RV ET Sbjct: 7 LSVKLPGLDLKNPIIPASGCFGFGEEYAKYYDLNKLGSIMVKATTLHPRFGNPTPRVAET 66 Query: 64 ASGMLNSVGLENPGVEAFLAKELPYWE--MLDTARLVNLGGNTLPDYVLGAELIQRDADA 121 ASGMLN++GL+NPG+E +A++LP+ D + N+ G+ DYV + + DA Sbjct: 67 ASGMLNAIGLQNPGLEVIMAEKLPWLNENFPDLPIIANVAGSEEDDYV---AVCAKIGDA 123 Query: 122 RSAAGKPAVDMIELNISCPNVKEGGIAFGVKTPAAQEXXXXXXXXTKLPLAVKLSPNAED 181 P V +IELNISCPNVK GG AFG A +K+PL VKLSPN D Sbjct: 124 ------PNVKVIELNISCPNVKHGGQAFGTDPDVAAALVKACKAVSKVPLYVKLSPNVTD 177 Query: 182 IAEMAVMCQEEGADAISLINTISGMKIDVRRRRSVFNNLYAGLSGPAIKPVALRMVHQVA 241 I +A + GAD +++INT+ G++ D++ R+ V N+ GLSGPAIKPVAL+++HQVA Sbjct: 178 IVPIAKAVEAAGADGLTMINTLMGVRFDLKTRKPVLANITGGLSGPAIKPVALKLIHQVA 237 Query: 242 KRVSIPVIGMGGITSATDIIEFIMAGATVIQVGTYNFMNLRAGSTLVDELQQFMIEENIS 301 + V IP+IGMGG+ SA D++E MAGA+ + VGT NF + ++++L + M + I Sbjct: 238 QVVDIPIIGMGGVESAQDVLEMYMAGASAVAVGTANFADPFVCPKIIEKLPEVMDQYGID 297 Query: 302 SLEEI 306 SLE + Sbjct: 298 SLENL 302 >SP0964 [F] COG0167 Dihydroorotate dehydrogenase Length = 312 Score = 232 bits (592), Expect = 5e-61, Method: Compositional matrix adjust. Identities = 123/308 (39%), Positives = 188/308 (61%), Gaps = 13/308 (4%) Query: 4 MTANIAGVHFKNPVIMASGTFGFGREYSKLYDVSLLGGISGKGLTLHPKAGNPGVRVYET 63 + ++ G+ KNP+I ASG FGFG+EY+K YD+ LLG I K TL P+ GNP RV ET Sbjct: 6 LQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSIMIKATTLEPRFGNPTPRVAET 65 Query: 64 ASGMLNSVGLENPGVEAFLAKELPYWE--MLDTARLVNLGGNTLPDYVLGAELIQRDADA 121 +GMLN++GL+NPG+E LA++LP+ E + + N+ G + +Y + I + + Sbjct: 66 PAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNV 125 Query: 122 RSAAGKPAVDMIELNISCPNVKE--GGIAFGVKTPAAQEXXXXXXXXTKLPLAVKLSPNA 179 ++ IELNISCPNV G+ G A + +++P+ VKL+P+ Sbjct: 126 KA---------IELNISCPNVDHCNHGLLIGQDPDLAYDVVKAAVEASEVPVYVKLTPSV 176 Query: 180 EDIAEMAVMCQEEGADAISLINTISGMKIDVRRRRSVFNNLYAGLSGPAIKPVALRMVHQ 239 DI +A ++ GA +++INT+ GM+ D++ R+ + N G+SGPA+ PVAL+++ Q Sbjct: 177 TDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQ 236 Query: 240 VAKRVSIPVIGMGGITSATDIIEFIMAGATVIQVGTYNFMNLRAGSTLVDELQQFMIEEN 299 VA+ +P+IGMGG+ SA +E +AGA+ I VGT NF N A +++ L + M + Sbjct: 237 VAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYG 296 Query: 300 ISSLEEIR 307 ISSLEE+R Sbjct: 297 ISSLEELR 304 >STM2187_1 [F] COG0167 Dihydroorotate dehydrogenase Length = 326 Score = 99.8 bits (247), Expect = 6e-21, Method: Compositional matrix adjust. Identities = 91/330 (27%), Positives = 150/330 (45%), Gaps = 39/330 (11%) Query: 3 NMTANIAGVHFKNPVIMASGTFGFGRE-YSKLYDVSLLGGISGKGLTLHPKAGNPGVRVY 61 +++ GV F NP ++S G E +K YD GGI K + G + Sbjct: 5 DLSVTFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGW-GGIVFKTI---------GFFIA 54 Query: 62 ETASGMLNSVGLENPGVEAF----LAKELPYWEMLDTARLVNLGGNTLPDYVLGAELI-- 115 S + + E+ G F E P E L R + PD VL A ++ Sbjct: 55 NEVSPRFDHLTKEDTGFIGFKNMEQIAEHPLEENLAAIRRLK---QDYPDKVLIASIMGE 111 Query: 116 --QRDADARSAAGKPAVDMIELNISCPNVKEGGIAFGV-KTPA-AQEXXXXXXXXTKLPL 171 Q+ + + DMIE N SCP + + V ++P ++ + LP+ Sbjct: 112 NEQQWQELARLVEEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYCRAVKRGSSLPM 171 Query: 172 AVKLSPNAEDIAEMAVMCQEEGADAISLINTISGM-KIDVRRR--RSVFN--NLYAGLSG 226 K++PN D+ E+A+ + GAD I+ INT+ + ID+ R+ V N + +G SG Sbjct: 172 LAKMTPNIGDMCEVALAAKRGGADGIATINTVKSITNIDLNRKIGMPVVNGKSSISGYSG 231 Query: 227 PAIKPVALRMVHQVAKRVSI---PVIGMGGITSATDIIEFIMAGATVIQVGT----YNFM 279 A+KP+ALR + Q+ + P+ G+GGI + D EF++ GA +QV T Y + Sbjct: 232 KAVKPIALRFIQQLRMHPELRDFPISGIGGIETWEDAAEFLLLGAATLQVTTGIMQYGY- 290 Query: 280 NLRAGSTLVDELQQFMIEENISSLEEIRGI 309 R + L ++ ++ +SL+E+ G+ Sbjct: 291 --RIVEDMASGLSHYLADQGFASLQEMIGL 318 >ZyeiA_1 [F] COG0167 Dihydroorotate dehydrogenase Length = 328 Score = 95.1 bits (235), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 88/330 (26%), Positives = 149/330 (45%), Gaps = 39/330 (11%) Query: 3 NMTANIAGVHFKNPVIMASGTFGFGRE-YSKLYDVSLLGGISGKGLTLHPKAGNPGVRVY 61 +++ GV F NP ++S G E +K YD GG+ K + G + Sbjct: 7 DLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGW-GGVVFKTI---------GFFIA 56 Query: 62 ETASGMLNSVGLENPGVEAF----LAKELPYWEMLDTARLVNLGGNTLPDYVLGAELI-- 115 S + + E+ G F E P E L R + PD VL A ++ Sbjct: 57 NEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLK---EDYPDKVLIASIMGE 113 Query: 116 --QRDADARSAAGKPAVDMIELNISCPNVKEGGIAFGV-KTPA-AQEXXXXXXXXTKLPL 171 Q+ + + DMIE N SCP + + V ++P ++ + LP+ Sbjct: 114 NEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYCRAVKRGSTLPM 173 Query: 172 AVKLSPNAEDIAEMAVMCQEEGADAISLINTISGM-KIDVRRR--RSVFN--NLYAGLSG 226 K++PN D+ E+A+ + GAD I+ INT+ + ID+ ++ + N + +G SG Sbjct: 174 LAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMPIVNGKSSISGYSG 233 Query: 227 PAIKPVALRMVHQVAKRVSI---PVIGMGGITSATDIIEFIMAGATVIQVGT----YNFM 279 A+KP+ALR + Q+ + P+ G+GGI + D EF++ GA +QV T Y + Sbjct: 234 KAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGAATLQVTTGIMQYGY- 292 Query: 280 NLRAGSTLVDELQQFMIEENISSLEEIRGI 309 R + L ++ ++ SL+E+ G+ Sbjct: 293 --RIVEDMASGLSHYLADQGFDSLQEMVGL 320 Score = 95.1 bits (235), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 88/330 (26%), Positives = 149/330 (45%), Gaps = 39/330 (11%) Query: 3 NMTANIAGVHFKNPVIMASGTFGFGRE-YSKLYDVSLLGGISGKGLTLHPKAGNPGVRVY 61 +++ GV F NP ++S G E +K YD GG+ K + G + Sbjct: 7 DLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGW-GGVVFKTI---------GFFIA 56 Query: 62 ETASGMLNSVGLENPGVEAF----LAKELPYWEMLDTARLVNLGGNTLPDYVLGAELI-- 115 S + + E+ G F E P E L R + PD VL A ++ Sbjct: 57 NEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLK---EDYPDKVLIASIMGE 113 Query: 116 --QRDADARSAAGKPAVDMIELNISCPNVKEGGIAFGV-KTPA-AQEXXXXXXXXTKLPL 171 Q+ + + DMIE N SCP + + V ++P ++ + LP+ Sbjct: 114 NEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYCRAVKRGSTLPM 173 Query: 172 AVKLSPNAEDIAEMAVMCQEEGADAISLINTISGM-KIDVRRR--RSVFN--NLYAGLSG 226 K++PN D+ E+A+ + GAD I+ INT+ + ID+ ++ + N + +G SG Sbjct: 174 LAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMPIVNGKSSISGYSG 233 Query: 227 PAIKPVALRMVHQVAKRVSI---PVIGMGGITSATDIIEFIMAGATVIQVGT----YNFM 279 A+KP+ALR + Q+ + P+ G+GGI + D EF++ GA +QV T Y + Sbjct: 234 KAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGAATLQVTTGIMQYGY- 292 Query: 280 NLRAGSTLVDELQQFMIEENISSLEEIRGI 309 R + L ++ ++ SL+E+ G+ Sbjct: 293 --RIVEDMASGLSHYLADQGFDSLQEMVGL 320 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.318 0.136 0.383 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 21,983,211 Number of extensions: 956594 Number of successful extensions: 3057 Number of sequences better than 1.0e-05: 16 Number of HSP's gapped: 3022 Number of HSP's successfully gapped: 16 Length of query: 310 Length of database: 38,378,431 Length adjustment: 106 Effective length of query: 204 Effective length of database: 26,153,027 Effective search space: 5335217508 Effective search space used: 5335217508 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 116 (49.3 bits)