BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0402.0005.p (583 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin2231 [G] COG0366 Glycosidases 510 e-144 BH2927 [G] COG0366 Glycosidases 506 e-143 BS_yvdF [G] COG0366 Glycosidases 474 e-133 L128694 [G] COG0366 Glycosidases 438 e-122 SPy1304 [G] COG0366 Glycosidases 354 2e-97 >lin2231 [G] COG0366 Glycosidases Length = 591 Score = 510 bits (1313), Expect = e-144, Method: Compositional matrix adjust. Identities = 267/592 (45%), Positives = 357/592 (60%), Gaps = 27/592 (4%) Query: 1 MLLEAMYHVPRDKWAYAYDAKTIHLRVRTKRDDVDCVVALTGDKY-----NWQDTSYDIM 55 M +YH P +AY+YDAKT+H+R+RTKR D+ V + D Y WQ SY Sbjct: 1 MEKAGIYHQPASSYAYSYDAKTLHIRIRTKRLDISKVTLIAADPYLWKNEKWQSESYS-- 58 Query: 56 MDKAASDDKFDYWEAAVRPKYKRLSYTFRVSKGN-ESVYLLDNGI--QTEYP-APPNHYY 111 M K A ++ DYW A+ P+++RL Y F ++ E+ + G TE A ++Y+ Sbjct: 59 MRKIAETEEHDYWFFAITPEHRRLQYGFLLTDTEGETTFYGGRGFFEPTEANLATMDYYF 118 Query: 112 EFPYIHQIDLFQVPNWAKDAVFYQIMTERFANGDPANDPEGTEPWGGT-PKLDNFFGGDL 170 +FP+IH +D F+ P W + ++YQI ERFANG+PA PE PWG P +FFGGDL Sbjct: 119 KFPFIHAVDTFEAPEWVGNTIWYQIFPERFANGNPAISPENALPWGSKDPNTTDFFGGDL 178 Query: 171 QGVLDHLDDLQELGINAIYFTPLFVSPSNHKYDIVDYKRVDPHFGDNELLKTVIEECHLR 230 +G++ HLD L ELGIN +Y TP+F +P+NHKYD +DYK++DPHFGD E + +++E H R Sbjct: 179 EGIIQHLDYLAELGINGVYLTPVFEAPTNHKYDTIDYKKIDPHFGDKETFRKLVQEAHKR 238 Query: 231 GIRVMLDAVFNHCSDKFPPFQDVLEKGEYSVYKDWFHVNSFPAEI-----VGGIPT--YD 283 GIR+MLDAVFNH D +QDV+EK E S Y+DWFH++SFP + G PT YD Sbjct: 239 GIRIMLDAVFNHIGDTSLEWQDVVEKEEKSAYRDWFHIHSFPVRQNENGNIEGEPTLSYD 298 Query: 284 TFGFFGNMPKFNTANHEVKTYLLEVAEYWIREIKADGWRLDVANEVDHHXXXXXXXXXXX 343 TF F +MPK NTAN EV+ YLL++A YWIRE DGWRLDVANEVDH Sbjct: 299 TFAFTTHMPKLNTANPEVQKYLLDIATYWIREFDIDGWRLDVANEVDH--AFWKEFKKAV 356 Query: 344 XXXNPEAYIVGEVWSDSLTWLLGDQFDSVMNYPFSGTVLE-FFNGGMDGITFGHRIGALL 402 + YI+GE+W DS WLLGD+F +VMNYPF+ T++E F + I Sbjct: 357 QAEKEDIYILGEIWHDSWIWLLGDEFHAVMNYPFTQTIIENFIEEKITPEQMVSGINEQY 416 Query: 403 MRYPQQTNEVVFNMLGSHDTPRLLTVVGEDKRRLKLAVVFLFTFMGTPCIYYGDEIGLTG 462 MRYP Q NEV+FNML SHDT R+LT D+ ++K A+ F+F G+PCIYYG EIG+ G Sbjct: 417 MRYPNQVNEVMFNMLDSHDTARILTRANNDEDKVKQALAFMFAHTGSPCIYYGTEIGMDG 476 Query: 463 HEDPDCRKCMEWDEASQDRELYDFYKMMIALRKHNKA-LREGRFRILQACENDPCIVYER 521 DP CRKCMEWDE Q++++ F K +IALRK N+A + G L A + + Sbjct: 477 GNDPGCRKCMEWDETKQNQDMLAFTKQLIALRKDNQAIITSGELNWLGASSETGITAFSK 536 Query: 522 ADDVIHFTVWMNNSPEPRILSHPME---TDDWQD-ALTGDPVAPEDGMMHIS 569 + N + E + + E TD W + A+T + V P G + I Sbjct: 537 ELNGEKLHFLFNQAKEDKDFTISFENTATDIWNNRAVTENLVVPAKGFLVIK 588 >BH2927 [G] COG0366 Glycosidases Length = 578 Score = 506 bits (1302), Expect = e-143, Method: Compositional matrix adjust. Identities = 267/561 (47%), Positives = 343/561 (61%), Gaps = 13/561 (2%) Query: 5 AMYHVPRDKWAYAYDAKTIHLRVRTKRDDVDCVVALTGDKYNWQDTSYDIM---MDKAAS 61 +YH P+ + AYAYD T+H+R+RT ++ V V + GD Y+W + M S Sbjct: 4 TIYHRPKSEDAYAYDKSTMHIRLRTGKNHVRKVNLIYGDPYDWTKNQWVTSKKEMTPTGS 63 Query: 62 DDKFDYWEAAVRPKYKRLSYTFRVSKGNESVYLLDNGIQTEYPAPPNHYYEFPYIHQIDL 121 D + D+W V P Y+RL Y F + E++Y ++G + Y+ FP+++ DL Sbjct: 64 DAQHDFWFVEVTPPYRRLRYGFEIITKTETIYYGESGFLDTPSKDTSDYFCFPFMNPADL 123 Query: 122 FQVPNWAKDAVFYQIMTERFANGDPANDPEGTEPWG-GTPKLDNFFGGDLQGVLDHLDDL 180 F P W KD V+YQI ERFANGDP+ +PEGT PWG P N FGGDLQG++DHLD L Sbjct: 124 FSPPEWVKDTVWYQIFPERFANGDPSRNPEGTLPWGSAAPTPTNHFGGDLQGIIDHLDYL 183 Query: 181 QELGINAIYFTPLFVSPSNHKYDIVDYKRVDPHFGDNELLKTVIEECHLRGIRVMLDAVF 240 QELGIN IY TP+F + SNHKYD +DY +VDPHFGD K +++ECH RGIR+MLDAVF Sbjct: 184 QELGINGIYLTPIFEASSNHKYDTIDYLKVDPHFGDKTTFKRLVDECHKRGIRIMLDAVF 243 Query: 241 NHCSDKFPPFQDVLEKGEYSVYKDWFHVNSFPAEIVGGIPTYDTFGFFGNMPKFNTANHE 300 NH FPPFQDVL+ ++S Y DWFH+ FP P YDTF F MPKFNT N E Sbjct: 244 NHAGFYFPPFQDVLKNQQHSKYSDWFHLRDFPVR-AEEPPNYDTFAFTPFMPKFNTENEE 302 Query: 301 VKTYLLEVAEYWIREIKADGWRLDVANEVDHHXXXXXXXXXXXXXXNPEAYIVGEVWSDS 360 V+ YLL VA YWI+E DGWRLDVANEVDH P+ YI+GEVW +S Sbjct: 303 VREYLLNVATYWIKEFDIDGWRLDVANEVDH--AFWREFRKRVKALKPDLYILGEVWHNS 360 Query: 361 LTWLLGDQFDSVMNYPFSGTVLEFFNGGMDGIT-FGHRIGALLMRYPQQTNEVVFNMLGS 419 L WL GDQFD+VMNYP + +++F G F H I L YP +V FN+LGS Sbjct: 361 LPWLAGDQFDAVMNYPLTNAIIDFAGKQKIGQQRFEHVITQLTHMYPIHVQQVAFNLLGS 420 Query: 420 HDTPRLLTVVGEDKRRLKLAVVFLFTFMGTPCIYYGDEIGLTGHEDPDCRKCMEWDEASQ 479 HDTPR+LT D R+KL + LF+ GTPCIYYGDEIG+TG +DP CRKCM W+E Q Sbjct: 421 HDTPRILTQTDNDVARVKLQFLLLFSQRGTPCIYYGDEIGMTGGQDPGCRKCMVWEEEEQ 480 Query: 480 DRELYDFYKMMIALRKHNKALREGRFRILQACENDPC-IVYERADDVIHFTVWMNNSPEP 538 ++EL F K +I LRK KA G L END ++Y++ + +N++ +P Sbjct: 481 NQELLSFVKRLIQLRKQEKAF--GTEGDLTFVENDSSLLIYQKTYENQQLLFVINSTDQP 538 Query: 539 RILSHPME--TDDWQDALTGD 557 + P+ + D LTG+ Sbjct: 539 DSVQLPVSWTNRNANDLLTGE 559 >BS_yvdF [G] COG0366 Glycosidases Length = 589 Score = 474 bits (1220), Expect = e-133, Method: Compositional matrix adjust. Identities = 236/514 (45%), Positives = 323/514 (62%), Gaps = 15/514 (2%) Query: 1 MLLEAMYHVPRDKWAYAYDAKTIHLRVRTKRDDVDCVVALTGDKYNWQDTSYDIM---MD 57 M A++H P AY+YD +T+H+++RTK+ D D + + GD Y + D + M Sbjct: 2 MEYAAIHHQPFSTDAYSYDGRTVHIKIRTKKGDADHIRFIWGDPYEYNDGKWSANEQPMR 61 Query: 58 KAASDDKFDYWEAAVRPKYKRLSYTFRVSKGNESVYLLDNGI--QTEYPAPPNHYY-EFP 114 K A+ + DYW A V P ++RL Y F V+ +E ++ +G+ E HYY +FP Sbjct: 62 KIAATEMHDYWFAEVVPPFRRLQYAFVVTDDHEDIFFGSSGVCPYNEKTLETIHYYFKFP 121 Query: 115 YIHQIDLFQVPNWAKDAVFYQIMTERFANGDPANDPEGTEPWGGT-PKLDNFFGGDLQGV 173 ++H+ D FQ P W K V+YQI ERFANG P+ PWG P +++FFGGDLQG+ Sbjct: 122 FVHEADTFQAPEWVKSTVWYQIFPERFANGREDLSPKNALPWGSKDPDVNDFFGGDLQGI 181 Query: 174 LDHLDDLQELGINAIYFTPLFVSPSNHKYDIVDYKRVDPHFGDNELLKTVIEECHLRGIR 233 +D LD L++LG+N IY TP+F +PSNHKYD +DY +DPHFGD EL +T++ + H RG+R Sbjct: 182 VDKLDYLEDLGVNGIYLTPIFSAPSNHKYDTLDYFSIDPHFGDPELFRTLVSQLHQRGMR 241 Query: 234 VMLDAVFNHCSDKFPPFQDVLEKGEYSVYKDWFHVNSFPAEIVGGIPTYDTFGFFGNMPK 293 +MLDAVFNH P +QDV++ G+ S YKDWFH++SFP YD F F +MPK Sbjct: 242 IMLDAVFNHIGSASPQWQDVVKNGDQSRYKDWFHIHSFPV----TDDNYDRFAFTADMPK 297 Query: 294 FNTANHEVKTYLLEVAEYWIREIKADGWRLDVANEVDHHXXXXXXXXXXXXXXNPEAYIV 353 NTAN EV+ YLL++A YWIRE DGWRLDVANEVDH P+ YI+ Sbjct: 298 LNTANPEVQKYLLDIALYWIREFDIDGWRLDVANEVDH--VFWKTFRQAVSTEKPDVYIL 355 Query: 354 GEVWSDSLTWLLGDQFDSVMNYPFSGTVLEFF-NGGMDGITFGHRIGALLMRYPQQTNEV 412 GE+W + WL GD+F + MNYPF+ ++E+F + + HR+ A LM +Q NEV Sbjct: 356 GEIWHSAEPWLRGDEFHAAMNYPFTEPMIEYFADQTISASRMAHRVNAHLMNGMKQANEV 415 Query: 413 VFNMLGSHDTPRLLTVVGEDKRRLKLAVVFLFTFMGTPCIYYGDEIGLTGHEDPDCRKCM 472 +FN+L SHDT RLLT D+++ + + F+F G+PCIYYG EIGL G DP CRKCM Sbjct: 416 MFNLLDSHDTKRLLTRCRNDEKKARALLAFMFAQTGSPCIYYGTEIGLNGENDPLCRKCM 475 Query: 473 EWDEASQDRELYDFYKMMIALRKH-NKALREGRF 505 W++ Q++++ F K +IALRK N L EG Sbjct: 476 VWEKEKQNQDMLQFMKRLIALRKQENTLLTEGHL 509 >L128694 [G] COG0366 Glycosidases Length = 584 Score = 438 bits (1127), Expect = e-122, Method: Compositional matrix adjust. Identities = 233/524 (44%), Positives = 311/524 (59%), Gaps = 35/524 (6%) Query: 5 AMYHVPRDKWAYAYDAKTIHLRVRTKRDDVDCVVALTGDKYNWQ-----DTSY----DIM 55 A+YH P ++AY Y +H+R++T++ DV+ V GD Y+W+ +T Y + Sbjct: 5 AIYHRPESEFAYLYAKNLMHVRLQTQKSDVEKVELFYGDPYSWEGLDNPETQYWASQKVE 64 Query: 56 MDKAASDDKFDYWEAAVRPKYKRLSYTFRVSK-GNESVYLLDNG--------IQTEYPAP 106 M K S + DY+EA + KR+ Y F ++ E V D G + +Y A Sbjct: 65 MKKYLSTNISDYFEAEITVATKRVDYLFVITGLDGEKVVYTDQGMFDYDESLVTKKYGA- 123 Query: 107 PNHYYEFPYIHQIDLFQVPNWAKDAVFYQIMTERFANGDPANDPEGTEPWG--GTPKLDN 164 + PY H+ D F+ P W K+ V+YQI ERFANGD +NDPEGT+ W P + Sbjct: 124 ----FRLPYFHESDRFKAPEWVKETVWYQIFPERFANGDTSNDPEGTKAWNPQDVPDRQD 179 Query: 165 FFGGDLQGVLDHLDDLQELGINAIYFTPLFVSPSNHKYDIVDYKRVDPHFGDNELLKTVI 224 F+GGDLQGV+DHL+ L ELG+N IYFTP+F + SNHKYD DY +D FGD EL KT++ Sbjct: 180 FYGGDLQGVIDHLEHLTELGVNGIYFTPIFQAFSNHKYDTEDYMEIDKQFGDKELFKTLV 239 Query: 225 EECHLRGIRVMLDAVFNHCSDKFPPFQDVLEKGEYSVYKDWFHVNSFPAEIV-------G 277 +E H RGI+VMLDAVFNH D +QDVL+ E S + DWFHVNS+PA Sbjct: 240 KEAHARGIKVMLDAVFNHIGDTSAQWQDVLKNQEKSKFADWFHVNSWPATYTPTDDFENA 299 Query: 278 GIPTYDTFGFFGNMPKFNTANHEVKTYLLEVAEYWIREIKADGWRLDVANEVDHHXXXXX 337 TYDTF F +MPK NTAN EV+ YLL+VA+YWI E D WRLDVA+EVDH Sbjct: 300 ENATYDTFAFTPHMPKLNTANPEVEAYLLKVADYWISEFDIDAWRLDVADEVDH--TFWK 357 Query: 338 XXXXXXXXXNPEAYIVGEVWSDSLTWLLGDQFDSVMNYPFSGTVLE-FFNGGMDGITFGH 396 + YI+GEVW + WL+GD+F +VMNY ++ + + + Sbjct: 358 KFRTTCDAAKDDFYILGEVWHSAQPWLVGDEFSAVMNYAYTDAIKDGLITKKISLEQMVS 417 Query: 397 RIGALLMRYPQQTNEVVFNMLGSHDTPRLLTVVGEDKRRLKLAVVFLFTFMGTPCIYYGD 456 I L Y +Q N+++FN+L SHDTPR+LTV +K +KL F F G PCIYYGD Sbjct: 418 NINTQLTLYRKQVNQMMFNVLDSHDTPRILTVAKNNKNLMKLVETFTFMQPGVPCIYYGD 477 Query: 457 EIGLTGHEDPDCRKCMEWDEASQDRELYDFYKMMIALRKHNKAL 500 E +TG DP+CRK M W+E QD E++DF+K +IALRK + + Sbjct: 478 EYAVTGGMDPECRKVMPWEEKDQDLEMFDFFKNLIALRKQYQGI 521 >SPy1304 [G] COG0366 Glycosidases Length = 567 Score = 354 bits (909), Expect = 2e-97, Method: Compositional matrix adjust. Identities = 199/539 (36%), Positives = 298/539 (55%), Gaps = 13/539 (2%) Query: 1 MLLEAMYHVPRDKWAYAYDAKTIHLRVRTKRDDVDCVVALT-GDKYNWQDTSYDIMMDKA 59 M + M H+P ++ +A K + +R+R ++D V L G KY + + ++ M A Sbjct: 1 MNVAGMLHIPDSRYCFAISQKELVIRLRVAKEDRGITVNLVHGPKYGYHEFQEELEMSVA 60 Query: 60 ASDDKFDYWEAAVRPKYKRLSYTFRVSKGNESVYLLDNGIQTEYPAPPNHY--YEFPYIH 117 +D Y+E + RL+Y F++ + ++ Y ++G+ T Y Y ++ PYI+ Sbjct: 61 YTDHTHAYYETTLTLDDVRLAYIFKIEEEGQTYYFSEDGLTTSYDFSNGFYNFFQMPYIN 120 Query: 118 QIDLFQVPNWAKDAVFYQIMTERFANGD-PANDPEGTEPWGGTPKLDNFFGGDLQGVLDH 176 +D+ QV W KDAVFYQI +RF GD D W P +F GGDL+G+ + Sbjct: 121 AVDVHQVIPWTKDAVFYQIFVDRFNRGDWEKGDSYVNMGWLEKPTPKSFAGGDLKGITEK 180 Query: 177 LDDLQELGINAIYFTPLFVSPSNHKYDIVDYKRVDPHFGDNELLKTVIEECHLRGIRVML 236 LD L++LGI IY TP+F S SNHKYDI DY +DP FG L+ +I+ H GI+++L Sbjct: 181 LDYLKDLGITVIYLTPIFQSISNHKYDISDYYAIDPQFGTKYDLQELIDLAHKMGIKIIL 240 Query: 237 DAVFNHCSDKFPPFQDVLEKGEYSVYKDWFHVNSFPAEIVGGIPTYDTFGFFGNMPKFNT 296 DAVFNH S FQDVL G+ S + DWF + + + Y+TF MPK+NT Sbjct: 241 DAVFNHASSDAVEFQDVLRYGKESKFFDWFMTHDEHPSM--DLVNYETFAGCNYMPKWNT 298 Query: 297 ANHEVKTYLLEVAEYWIREIKADGWRLDVANEVDHHXXXXXXXXXXXXXXNPEAYIVGEV 356 +N +V+ YL+E+ YWI+E DGWRLDV++EV H +A ++GE Sbjct: 299 SNRDVQDYLIEIGRYWIKEFCIDGWRLDVSDEVSH--DFWRRFRQAVKAEKADAILIGEN 356 Query: 357 WSDSLTWLLGDQFDSVMNYPFSGTVLEFFN-GGMDGITFGHRIGALLMRYPQQTNEVVFN 415 W D+ +L GDQ+D +MNY F+ L++F M ++ +LMR Q N + N Sbjct: 357 WHDAYPYLAGDQYDGIMNYAFTKACLDYFAFETMTSKQLAEKLSNILMRNTWQVNRMNLN 416 Query: 416 MLGSHDTPRLLTVVGEDKRRLKLAVVFLFTFMGTPCIYYGDEIGLTGHEDPDCRKCMEWD 475 +L SHDT R T V K +L A+ LF FMG PC+YYG E+ + G DPD R+ WD Sbjct: 417 LLDSHDTHRFFTQVKGSKDKLLAALALLFIFMGIPCLYYGTEVAMEGGYDPDSRRGFPWD 476 Query: 476 EASQDRELYDFYKMMIALRKHNKALREGRFRILQACENDPCIVYERADDVIHFTVWMNN 534 +A +D++ + K +IALR+ AL+EG I ++ C++ +R+ ++ +NN Sbjct: 477 DAERDKDFFSKVKAIIALRQQ-PALQEGAISI---DYDEDCLILKRSLGEQSISLVLNN 531 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.322 0.141 0.453 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 47,920,108 Number of extensions: 2195922 Number of successful extensions: 3551 Number of sequences better than 1.0e-05: 104 Number of HSP's gapped: 3326 Number of HSP's successfully gapped: 108 Length of query: 583 Length of database: 38,378,431 Length adjustment: 112 Effective length of query: 471 Effective length of database: 25,461,023 Effective search space: 11992141833 Effective search space used: 11992141833 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 119 (50.4 bits)