BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0401.0003.p (325 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0653 [S] COG1511 Predicted membrane protein 297 1e-80 L118462 [S] COG1511 Predicted membrane protein 225 8e-59 SA2436 [S] COG1511 Predicted membrane protein 222 8e-58 CAC2582 [S] COG1511 Predicted membrane protein 153 4e-37 CAC2583 [S] COG1511 Predicted membrane protein 140 2e-33 >lin0653 [S] COG1511 Predicted membrane protein Length = 896 Score = 297 bits (761), Expect = 1e-80, Method: Compositional matrix adjust. Identities = 152/318 (47%), Positives = 211/318 (66%), Gaps = 3/318 (0%) Query: 9 QNGLPAAGEHIHAAAEFARKDLPGAEEKFRRASEWITAGLPRADRGVNRAAELVRNDLPA 68 QN P +I AA F R DLPG E++ +AS I +P ++ V AA L R +LP Sbjct: 579 QNDYPNVKANIKKAANFIRDDLPGLEKEINQASNLIQEKMPEFEKAVKIAANLSREELPE 638 Query: 69 LEHAVRRAADTVRKVKREVNLEEIAQLLGGDIKGTSDFLASPVVLKEKALYPIPNYGSAM 128 E A+ AA+ + + +L+ I ++L D S F+A+PV LKE + YPIPNYGSA Sbjct: 639 FEKAINNAANKITDFDKNYDLQSIIKMLRNDADKDSSFIANPVNLKETSYYPIPNYGSAS 698 Query: 129 TPFYVVLSLWVGGTLLISLLRIGVDT-GGIPYXXXXYFGRLLTFLTVGIFQALIAVLGNM 187 +PFY L LWVG LLISLLR+ V+ GI YFGRLLTFL++G+ QALI LGN+ Sbjct: 699 SPFYTALCLWVGALLLISLLRVDVEVPAGIFSHYHRYFGRLLTFLSIGLMQALIVTLGNI 758 Query: 188 YLLKCYVADKLWFVLFAVLISIVFVTIVFTLVYVFGNLGKGIAIVFMVLQFSSSGGTFPI 247 +LL +A+ L VLF++ IS+VF+TIV+TLV +F N+GKGIAI+ +VLQ S +GG FPI Sbjct: 759 FLLGVSIAEPLLHVLFSMFISVVFMTIVYTLVSLFNNVGKGIAIILLVLQISGAGGNFPI 818 Query: 248 STTGRFFQILNPFMPFTYAISLLREAVGGI-LPEVAVRDALYLVLFGMLALVLALTLQKP 306 + FFQ + PF+PFTYA+SL+RE+VGG+ +P V + D L F +L + L L L+KP Sbjct: 819 QVSPPFFQAIYPFLPFTYAVSLIRESVGGLYMPTVWI-DMSVLAGFAILFIALGLLLKKP 877 Query: 307 LERFIRRAAEQAEASRLI 324 L++ + + +E+A+ S+LI Sbjct: 878 LDKVVPKLSEKAKRSKLI 895 >L118462 [S] COG1511 Predicted membrane protein Length = 901 Score = 225 bits (573), Expect = 8e-59, Method: Compositional matrix adjust. Identities = 113/273 (41%), Positives = 166/273 (60%), Gaps = 4/273 (1%) Query: 9 QNGLPAAGEHIHAAAEFARKDLPGAEEKFRRASEWITAGLPRADRGVNRAAELVRNDLPA 68 +N P + A++F + D G + +P + +N+A +L++ND P Sbjct: 581 KNEFPTLKSKLGTASDFFKNDYAGVRKDLTDTLTMANDKMPDVESALNQANDLIQNDWPE 640 Query: 69 LEHAVRRAADTVRKVKREVNLEEIAQLLGGDIKGTSDFLASPVVLKEKALYPIPNYGSAM 128 ++ +++AA ++K ++EV+L EI +LL D SDFL PV ++E +YPI N GSA Sbjct: 641 IKSGIQKAAAAIQKGEKEVDLGEIVKLLKLDATKESDFLTQPVEVQENQIYPIANNGSAS 700 Query: 129 TPFYVVLSLWVGGTLLISLLRIGV---DTGGIPYXX-XXYFGRLLTFLTVGIFQALIAVL 184 TPFY L LWVG LL SL V D Y +F R+ +F+ +G+ QALI L Sbjct: 701 TPFYTALCLWVGAVLLSSLATTEVHLSDKDKKRYSKREQFFARMGSFVAIGLAQALIVTL 760 Query: 185 GNMYLLKCYVADKLWFVLFAVLISIVFVTIVFTLVYVFGNLGKGIAIVFMVLQFSSSGGT 244 GN + L V D + VLFA+LISI F+ +V+ LV +FGN+GKGIAI+ +VL S GG Sbjct: 761 GNYFGLGVDVRDPFYSVLFALLISIAFMIMVYVLVALFGNVGKGIAIIILVLSISGGGGN 820 Query: 245 FPISTTGRFFQILNPFMPFTYAISLLREAVGGI 277 +PI +G+FFQ +NP++PFT+A++LLRE+ GGI Sbjct: 821 YPIQVSGKFFQAINPYLPFTHAVNLLRESAGGI 853 >SA2436 [S] COG1511 Predicted membrane protein Length = 993 Score = 222 bits (565), Expect = 8e-58, Method: Composition-based stats. Identities = 114/336 (33%), Positives = 187/336 (55%), Gaps = 21/336 (6%) Query: 9 QNGLPAAGEHIHAAAEFARKDLPGAEEKFRRASEWITAGLP------------------R 50 Q P + AA F R DLP E++ A+ + LP + Sbjct: 656 QANFPKVKSDVAKAANFVRNDLPQLEQRLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQ 715 Query: 51 ADRGVNRAAELVRNDLPALEHAVRRAADTVRKVKREVNLEEIAQLLGGDIKGTSDFLASP 110 A + ++ A+ +N LP +E +++A +K+ ++ ++++ L D+K + +A+P Sbjct: 716 AKKALSDLADFSQNKLPDVEKDLKKANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANP 775 Query: 111 VVLKEKALYPIPNYGSAMTPFYVVLSLWVGGTLLISLLRIGVDTGGIPYXXX---XYFGR 167 + K ++P+ +YGS MTPFY LS+WVG L++SLL + + + G+ Sbjct: 776 INKKTVDVFPVKDYGSGMTPFYTALSVWVGALLMVSLLTVDNKHKSLESVLTTRQVFLGK 835 Query: 168 LLTFLTVGIFQALIAVLGNMYLLKCYVADKLWFVLFAVLISIVFVTIVFTLVYVFGNLGK 227 F+ +G+ QALI +G++ +LK V + FVL + SI+F +IV+T V + GN GK Sbjct: 836 AGFFIMLGMLQALIVSVGDLLILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGK 895 Query: 228 GIAIVFMVLQFSSSGGTFPISTTGRFFQILNPFMPFTYAISLLREAVGGILPEVAVRDAL 287 IAIV +VLQ + GGTFPI TT +FFQ ++P++PFTYAI LRE VGGI+PE+ + + Sbjct: 896 AIAIVLLVLQIAGGGGTFPIQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLI 955 Query: 288 YLVLFGMLALVLALTLQKPLERFIRRAAEQAEASRL 323 L LFG+ V+ L L+ + ++R +E+ + S + Sbjct: 956 ILTLFGIGFFVVGLILKPVTDPLMKRVSEKVDQSNV 991 >CAC2582 [S] COG1511 Predicted membrane protein Length = 722 Score = 153 bits (386), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 88/237 (37%), Positives = 141/237 (59%), Gaps = 10/237 (4%) Query: 85 REVNLEEIAQLL-----GGDIKGTSDFLASPVVLKEKALYPIPNYGSAMTPFYVVLSLWV 139 +E N ++ Q++ D G +F+++P +K++++Y IPNYGS M P Y VL++WV Sbjct: 476 QETNNNDLIQIISILQSNPDFMG--NFISTPFSIKDESIYSIPNYGSGMAPVYTVLAIWV 533 Query: 140 GGTLLISLLRIGV-DTGGIPYXX--XXYFGRLLTFLTVGIFQALIAVLGNMYLLKCYVAD 196 G LL+SLL+ V D+ G+ +FG++LTF+T+ + Q+ I LG++ LLK Y + Sbjct: 534 GTLLLVSLLKTRVPDSQGLHILTLREKHFGKMLTFITLSLIQSFIVSLGDILLLKIYTVN 593 Query: 197 KLWFVLFAVLISIVFVTIVFTLVYVFGNLGKGIAIVFMVLQFSSSGGTFPISTTGRFFQI 256 + FA++ F IVFTLV GN+GK I+I+F+++Q + SG T+PI FF+I Sbjct: 594 PALLIAFALMSGFTFCVIVFTLVSTLGNIGKAISIIFLIIQIAGSGSTYPIQVDPLFFRI 653 Query: 257 LNPFMPFTYAISLLREAVGGILPEVAVRDALYLVLFGMLALVLALTLQKPLERFIRR 313 L P +PFTY++ REA+ G L + D L+L ++ + + PL R I + Sbjct: 654 LQPMLPFTYSVGGFREAIAGPLISSVMLDFTALILISVVFIFFGFFFKIPLHRPISK 710 >CAC2583 [S] COG1511 Predicted membrane protein Length = 709 Score = 140 bits (354), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 87/273 (31%), Positives = 145/273 (53%), Gaps = 6/273 (2%) Query: 54 GVNRAAELVRNDLPALEHAVRRAADTVRKVKREVNLEEIAQLLGGDIKGTSDFLASPVVL 113 +N+A + + + L +L + R + + + + +L +I +LL + + F++SP+ Sbjct: 440 SINQAND-INSKLSSLSGELNRLSGKMNSL-SDSDLNDIIKLLSMNTNELASFISSPLTT 497 Query: 114 KEKALYPIPNYGSAMTPFYVVLSLWVGGTLLISLLRIGVDT-GGIPYXX--XXYFGRLLT 170 +E +Y +G +TPFY VL++WVG LL S+L G + +FG++L Sbjct: 498 REVDIYGEGVFGVGLTPFYSVLAIWVGVLLLSSMLTTEFKNLSGREHLNIWQEHFGKMLL 557 Query: 171 FLTVGIFQALIAVLGNMYLLKCYVADKLWFVLFAVLISIVFVTIVFTLVYVFGNLGKGIA 230 FL + + Q I +LG+ Y+L + L + F + S+ F I+FTLV +FGN+GK IA Sbjct: 558 FLVLSLIQTTIIILGDKYILHVNPQNMLLLMCFGWVCSVTFTFIIFTLVAIFGNVGKAIA 617 Query: 231 IVFMVLQFSSSGGTFPISTTGRFFQILNPFMPFTYAISLLREAVGGILPEVAVRDALYLV 290 +V MV Q + SGG +PI T + F IL P PFTY I+ REA+ G + + L Sbjct: 618 VVMMVFQIAGSGGIYPIQTNPKIFGILEPLWPFTYGINGFREAISGPIWNNVYSNLRNLG 677 Query: 291 LFGMLALVLALTLQKPLERFIRRAAEQAEASRL 323 F + L + L++P + R + + S+L Sbjct: 678 FFIIFFFFLTI-LKRPFHKLTRAMEHKFKESQL 709 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.327 0.143 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 23,018,306 Number of extensions: 975148 Number of successful extensions: 2764 Number of sequences better than 1.0e-05: 11 Number of HSP's gapped: 2757 Number of HSP's successfully gapped: 11 Length of query: 325 Length of database: 38,378,431 Length adjustment: 107 Effective length of query: 218 Effective length of database: 26,037,693 Effective search space: 5676217074 Effective search space used: 5676217074 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 116 (49.3 bits)