BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0400.0004.p (205 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value lin0293 [G] COG0406 Fructose-2,6-bisphosphatase 122 3e-28 ECs0676 [G] COG0406 Fructose-2,6-bisphosphatase 107 9e-24 ZphpB [G] COG0406 Fructose-2,6-bisphosphatase 105 6e-23 phpB [G] COG0406 Fructose-2,6-bisphosphatase 105 6e-23 >lin0293 [G] COG0406 Fructose-2,6-bisphosphatase Length = 346 Score = 122 bits (307), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 15/184 (8%), Positives = 37/184 (20%), Gaps = 3/184 (1%) Query: 5 LYLTRHGETEWNVVHRMQGFEDSPLTALGVRQAESLKTALDAVPLDIVYTSPSPRAIRTA 64 + + ++ A +D + V P + + Sbjct: 8 HSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPETQGLTET 67 Query: 65 KLICGDRNIPLETSEALMEMGFGIWEGRVHADVEAQFPEQWNLFWNDPEKFA--ITDSET 122 + + G Q ++ N+ + TD Sbjct: 68 YVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKRLQTDYLD 127 Query: 123 YTQVHDRAIGFLNEIVQKHPGKSVLIVTHTIVVKMLMAHFAGSELKQLWK-SPEILPTSL 181 QVH + + +A + + + S E + Sbjct: 128 LYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQRAGKIRYIGVSNETAFGVM 187 Query: 182 SRID 185 + Sbjct: 188 RYLH 191 >ECs0676 [G] COG0406 Fructose-2,6-bisphosphatase Length = 346 Score = 107 bits (268), Expect = 9e-24, Method: Compositional matrix adjust. Identities = 11/169 (6%), Positives = 33/169 (19%) Query: 3 MKLYLTRHGETEWNVVHRMQGFEDSPLTALGVRQAESLKTALDAVPLDIVYTSPSPRAIR 62 M+ + H E + + + A +D+ P P Sbjct: 1 MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGINLIDVAEMYPVPPRPE 60 Query: 63 TAKLICGDRNIPLETSEALMEMGFGIWEGRVHADVEAQFPEQWNLFWNDPEKFAITDSET 122 T L L + ++ + + L + + + Sbjct: 61 TQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPDQALDRKNIREALHDSLKR 120 Query: 123 YTQVHDRAIGFLNEIVQKHPGKSVLIVTHTIVVKMLMAHFAGSELKQLW 171 + + + + + + + Sbjct: 121 LQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDTLDALAEYQR 169 >ZphpB [G] COG0406 Fructose-2,6-bisphosphatase Length = 292 Score = 105 bits (261), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 9/169 (5%), Positives = 35/169 (20%) Query: 3 MKLYLTRHGETEWNVVHRMQGFEDSPLTALGVRQAESLKTALDAVPLDIVYTSPSPRAIR 62 M T + ++ + GF+++ L + + R + Sbjct: 1 MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLMTRGVRNVIEMTNRYMG 60 Query: 63 TAKLICGDRNIPLETSEALMEMGFGIWEGRVHADVEAQFPEQWNLFWNDPEKFAITDSET 122 D + + H + + + T+ + Sbjct: 61 RNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELAQEMVDEIEQGIDGTELKA 120 Query: 123 YTQVHDRAIGFLNEIVQKHPGKSVLIVTHTIVVKMLMAHFAGSELKQLW 171 +++ + + + + + + Sbjct: 121 GIIAEIGTSEGKITPLEEKVFIAAALAHNQTGRPISTHTSFSTMGLEQL 169 Score = 105 bits (261), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 9/191 (4%), Positives = 35/191 (18%), Gaps = 1/191 (0%) Query: 3 MKLYLTRHGETEWNVVHRMQGFEDSPLTALGVRQAESLKTALDAVPLDIVYTSPSPRAIR 62 M T + ++ + GF+++ L + + R + Sbjct: 1 MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLMTRGVRNVIEMTNRYMG 60 Query: 63 TAKLICGDRNIPLETSEALMEMGFGIWEGRVHADVEAQFPEQWNLFWNDPEKFAITDSET 122 D + + H + + + T+ + Sbjct: 61 RNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELAQEMVDEIEQGIDGTELKA 120 Query: 123 YTQVHDRAIGFLNEIVQKHPGKSVLIVTHTIVVKMLMAHFAGSELKQLWKSPEILPTSLS 182 +++ + + + + + + Sbjct: 121 GIIAEIGTSEGKITPLEEKVFIAAALAHNQTGRPISTHTSFSTMGLEQLAL-LQAHGVDL 179 Query: 183 RIDITDDAAEI 193 Sbjct: 180 SRVTVGHCDLK 190 >phpB [G] COG0406 Fructose-2,6-bisphosphatase Length = 292 Score = 105 bits (261), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 9/191 (4%), Positives = 35/191 (18%), Gaps = 1/191 (0%) Query: 3 MKLYLTRHGETEWNVVHRMQGFEDSPLTALGVRQAESLKTALDAVPLDIVYTSPSPRAIR 62 M T + ++ + GF+++ L + + R + Sbjct: 1 MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLMTRGVRNVIEMTNRYMG 60 Query: 63 TAKLICGDRNIPLETSEALMEMGFGIWEGRVHADVEAQFPEQWNLFWNDPEKFAITDSET 122 D + + H + + + T+ + Sbjct: 61 RNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELAQEMVDEIEQGIDGTELKA 120 Query: 123 YTQVHDRAIGFLNEIVQKHPGKSVLIVTHTIVVKMLMAHFAGSELKQLWKSPEILPTSLS 182 +++ + + + + + + Sbjct: 121 GIIAEIGTSEGKITPLEEKVFIAAALAHNQTGRPISTHTSFSTMGLEQLAL-LQAHGVDL 179 Query: 183 RIDITDDAAEI 193 Sbjct: 180 SRVTVGHCDLK 190 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.319 0.134 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 15,479,421 Number of extensions: 577220 Number of successful extensions: 1315 Number of sequences better than 1.0e-05: 83 Number of HSP's gapped: 1257 Number of HSP's successfully gapped: 84 Length of query: 205 Length of database: 38,378,431 Length adjustment: 101 Effective length of query: 104 Effective length of database: 26,729,697 Effective search space: 2779888488 Effective search space used: 2779888488 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 113 (48.1 bits)