BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0400.0001.p (123 letters) Database: COG 115,334 sequences; 38,378,431 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value L178933 [S] COG0599 Uncharacterized homolog of gamma-carboxymuco... 168 2e-42 Cgl1022 [S] COG0599 Uncharacterized homolog of gamma-carboxymuco... 149 1e-36 YPO1999 [S] COG0599 Uncharacterized homolog of gamma-carboxymuco... 108 2e-24 SMa0559 [S] COG0599 Uncharacterized homolog of gamma-carboxymuco... 107 6e-24 RSp1472 [S] COG0599 Uncharacterized homolog of gamma-carboxymuco... 104 2e-23 >L178933 [S] COG0599 Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit Length = 248 Score = 168 bits (425), Expect = 2e-42, Method: Compositional matrix adjust. Identities = 6/102 (5%), Positives = 20/102 (19%) Query: 19 LEKQTAGRDNLGEFAPKFAELNDDVLFGEVWSREKELSPRDRSMITVATLISEGNFEQLT 78 + L E + ++L+ + + + + Sbjct: 1 MMFLPEQISKLNELETLVFDFIIKSPEEVQQMTIRDLASQAHVSTSTIVRLCTKLGFEGW 60 Query: 79 PHLHKAKANGITQEEIAEIITHLSFYAGWPKAWSAFNIAKQI 120 L N + ++ E + + Sbjct: 61 SDLKFYLKNQLKEKTPEEQHYDNMLEFNMFLRRMNTDNYQAK 102 >Cgl1022 [S] COG0599 Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit Length = 107 Score = 149 bits (376), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 69/104 (66%), Positives = 83/104 (79%), Gaps = 1/104 (0%) Query: 20 EKQTAGRDNLGEFAPKFAELNDDVLFGEVWSREKELSPRDRSMITVATLISEGNFEQ-LT 78 +K TAGRD LGEFAPK AELNDDVLFG+VWSRE ELSPRDRS++ V L++ G + Sbjct: 3 QKVTAGRDILGEFAPKSAELNDDVLFGQVWSRESELSPRDRSIVIVTMLMASGVLDSAFE 62 Query: 79 PHLHKAKANGITQEEIAEIITHLSFYAGWPKAWSAFNIAKQIFN 122 H+ +AK NG+T EEIAEIITH++FYAGWPKAW+AF IAK I+ Sbjct: 63 SHVQRAKDNGVTAEEIAEIITHVAFYAGWPKAWAAFRIAKDIYT 106 >YPO1999 [S] COG0599 Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit Length = 140 Score = 108 bits (271), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 46/95 (48%), Positives = 70/95 (73%), Gaps = 1/95 (1%) Query: 29 LGEFAPKFAELNDDVLFGEVWSREKELSPRDRSMITVATLISEGNFEQLTPHLHKAKANG 88 +G+ APK A+L DDVL+ ++W R +LS RDRS++TV+ L++ +QL HL A+ NG Sbjct: 45 MGDIAPKMAQLTDDVLYADIWERP-QLSKRDRSLVTVSALVAMDRPDQLRSHLRLARQNG 103 Query: 89 ITQEEIAEIITHLSFYAGWPKAWSAFNIAKQIFND 123 +T+EE+ E IT L+FY+GWP A +A +IAK++F + Sbjct: 104 VTEEELVEAITQLAFYSGWPTAVTAISIAKEVFKE 138 >SMa0559 [S] COG0599 Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit Length = 259 Score = 107 bits (266), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 49/102 (48%), Positives = 70/102 (68%), Gaps = 1/102 (0%) Query: 20 EKQTAGRDNLGEFAPKFAELNDDVLFGEVWSREKELSPRDRSMITVATLISEGNFEQLTP 79 ++ T + G AP + D LF ++W R +L+PRDRS++T+A LIS G EQ+T Sbjct: 154 QRATTVGNQFGSVAPGLVQYTTDYLFRDLWLR-PDLAPRDRSLVTIAALISVGQVEQITF 212 Query: 80 HLHKAKANGITQEEIAEIITHLSFYAGWPKAWSAFNIAKQIF 121 HL+KA NG+++E+ AE+ITHL+FYAGWP A SA +AK +F Sbjct: 213 HLNKALDNGLSEEQAAEVITHLAFYAGWPNAMSALPVAKAVF 254 Score = 74.3 bits (181), Expect = 4e-14, Method: Compositional matrix adjust. Identities = 36/95 (37%), Positives = 54/95 (56%), Gaps = 1/95 (1%) Query: 27 DNLGEFAPKFAELNDDVLFGEVWSREKELSPRDRSMITVATLISEGNFEQLTPHLHKAKA 86 D + AP + L+G+VW R L+ RDRS++T+A LI+ G LT + +A Sbjct: 34 DQVRSVAPALEQYTQQRLYGDVWQRPG-LNRRDRSLVTIAALIARGEAPALTYYADQALE 92 Query: 87 NGITQEEIAEIITHLSFYAGWPKAWSAFNIAKQIF 121 NG+ EI+E ITHL++Y+GW KA + + F Sbjct: 93 NGVKPSEISETITHLAYYSGWGKAMATVGPVSEAF 127 >RSp1472 [S] COG0599 Uncharacterized homolog of gamma-carboxymuconolactone decarboxylase subunit Length = 108 Score = 104 bits (260), Expect = 2e-23, Method: Compositional matrix adjust. Identities = 51/100 (51%), Positives = 68/100 (68%), Gaps = 1/100 (1%) Query: 22 QTAGRDNLGEFAPKFAELNDDVLFGEVWSREKELSPRDRSMITVATLISEGNFEQLTPHL 81 T RD G AP A+ D+VLFG+VW R LSPRDRS+ITVA+LI+ +L HL Sbjct: 5 STVARDAFGHIAPALADYTDNVLFGDVWQRPG-LSPRDRSLITVASLIALYRTNELPFHL 63 Query: 82 HKAKANGITQEEIAEIITHLSFYAGWPKAWSAFNIAKQIF 121 KA NG+ ++E+ E ITHL+FY+GWP A +A IA+++F Sbjct: 64 KKALDNGLRRDELIEAITHLAFYSGWPTASTALPIAQRVF 103 Database: COG Posted date: Aug 3, 2010 9:58 AM Number of letters in database: 38,378,431 Number of sequences in database: 115,334 Lambda K H 0.316 0.134 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 115334 Number of Hits to DB: 9,771,594 Number of extensions: 406177 Number of successful extensions: 632 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 628 Number of HSP's successfully gapped: 21 Length of query: 123 Length of database: 38,378,431 Length adjustment: 89 Effective length of query: 34 Effective length of database: 28,113,705 Effective search space: 955865970 Effective search space used: 955865970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 109 (46.6 bits)